scholarly journals Antibiotic Susceptibility Profiles of Listeria monocytogenes Strains Isolated from Food Products and Clinical Samples

2014 ◽  
Vol 22 (2) ◽  
Author(s):  
Marius Eduard Caplan ◽  
Lorena Andreea Mateescu ◽  
Tatiana Vassu Dimov ◽  
Alexandru Rafila ◽  
Alina Maria Borcan
Keyword(s):  
Listeria Monocytogenes ◽  
Antibiotic Susceptibility ◽  
Food Products ◽  
Clinical Samples ◽  
Susceptibility Profiles

Antimicrobial Susceptibility Pattern of Staphylococcus Species from Clinical Samples

2020 ◽  
Author(s):  
Muhammad Ali
Keyword(s):  
Clavulanic Acid ◽  
Antibiotic Susceptibility ◽  
Vaginal Swab ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Susceptibility Pattern ◽  
Mannitol Salt Agar ◽  
Agar Disc ◽  
Microbiological Methods ◽  
Amoxicillin Clavulanic Acid

The research was aimed to evaluate the antibiotic susceptibility pattern of Staphylococcus species from clinical samples obtained from some hospitals in Kano metropolis, Nigeria. The ear swab, high vaginal swab (HVS), wound swab and urine samples from the patients attending the hospitals were collected and inoculated onto the surface of freshly prepared Nutrient agar for bacterial isolation. The bacteria isolated were identified by conventional microbiological methods namely; Gram staining, biochemical test (such as catalase, coagulase, and DNase test), mannitol salt agar and heamolysis test. The isolates were subjected to antibiotic susceptibility testing using the agar disc diffusion method. The result showed that S. aureus was highly susceptible to Ciprofloxacin 105 (68.63%), Gentamicin 102 (66.67%), Levofloxacin 95 (62.08%) and Amikacin 90 (58.82%), S. epidermidis was highly susceptible to Gentamicin 13 (61.90%), Levofloxacin 12 (57.14%) and Nitrofurantoin 11 (52.38%) while S. saprophyticus was highly susceptible to Cefoxitin 7 (77.78%), Gentamicin 6 (66.67%) and Nitrofurantoin 5 (55.56%). On the other hand, S. aureus was highly resistant to Cefuroxime 153 (100%), Ceftazidime 150 (98.04%), Amoxicillin/clavulanic acid 120 (78.43%) and Cloxacillin 111 (72.55%), S. epidermidis was highly resistant to Ceftazidime 20 (95.24%), Cloxacillin 19 (90.48%) then Cefoxitin, Erythromycin and Amoxicillin/clavulanic acid with 15 (71.43%) both. S. saprophyticus was highly resistant to Cefepime 9 (100%), Cloxacillin 8 (88.89%), Ceftazidime 7 (77.78%), Imipenem and Erythromycin with 6 (66.67%) respectively. There is a statistical difference in the sensitivity of the isolates against the antibiotics used at p<0.05. It is concluded that Staphylococcus species develop resistance to some classes of antibiotics.

scholarly journals Prevalence of metallo-β-lactamase genes among Pseudomonas aeruginosa isolated from various clinical samples in China

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Wei Wang ◽  
Xiaoya Wang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Susceptibility ◽  
Ethylenediaminetetraacetic Acid ◽  
Carbapenem Resistance ◽  
Opportunistic Pathogen ◽  
Detection Methods ◽  
Clinical Samples ◽  
Routine Practice ◽  
Carbapenem Resistant ◽  
High Prevalence

AbstractBackgroundPseudomonas aeruginosa is an opportunistic pathogen which is associated with nosocomial infections and causes various diseases including urinary tract infection, pneumonia, soft-tissue infection and sepsis. The emergence of P. aeruginosa-acquired metallo-β-lactamase (MBL) is most worrisome and poses a serious threat during treatment and infection control. The objective of this study was to identify antibiotic susceptibility, phenotypic detection of MBL production and to determine the prevalence of MBL genes in carbapenem-resistant P. aeruginosa isolated from different clinical samples.MethodsA total of 329 non-duplicate P. aeruginosa isolated from various clinical samples from two hospitals in China between September 2017 and March 2019 were included in this study. Phenotypic detection of MBL was performed by the combined detection method using imipenem and imipenem-ethylenediaminetetraacetic acid (EDTA) discs. MBL-encoding genes including blaVIM-1, blaVIM-2, blaIMP-1, blaIMP-2, blaSPM-1, blaSIM, blaNDM-1 and blaGIM were detected by polymerase chain reaction (PCR).ResultsOf the 329 P. aeruginosa, majority of the isolates were resistant to imipenem (77.5%) followed by meropenem (64.7%). Of the 270 P. aeruginosa isolates tested, 149 (55.2%) isolates were found to be positive for MBL detection. Of the different samples, 57.8% (n = 26) of P. aeruginosa isolated from blood were found to be positive for MBL production. Of the various MBL genes, blaIMP-1 (28.2%) was the most predominant gene detected followed by blaVIM-2 (18.8%), blaVIM-1 (16.1%), blaNDM-1 (9.4%), blaIMP-2 (6.7%), blaSIM (6.0%), blaSPM-1 (4.0%) and blaGIM (1.3%) genes.ConclusionsThe high resistance of P. aeruginosa toward imipenem and meropenem and the high prevalence of blaIMP-1 and blaVIM-2 set the alarm on the increasing, perhaps the increased, carbapenem resistance. In addition to routine antibiotic susceptibility testings, our results emphasize the importance of both the phenotypic and genotypic MBL detection methods in routine practice for early detection of carbapenem resistance and to prevent further dissemination of this resistant pathogen.

scholarly journals Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents

2019 ◽  
Vol 79 (4) ◽  
pp. 555-565 ◽  
Author(s):  
A. F. G. Rave ◽  
A. V. Kuss ◽  
G. H. S. Peil ◽  
S. R. Ladeira ◽  
J. P. V. Villarreal ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Klebsiella Oxytoca ◽  
Effluent Treatment ◽  
Automated System ◽  
Resistant Bacteria ◽  
Biochemical Tests ◽  
Biochemical Identification ◽  
Identification Techniques ◽  
Susceptibility Profiles ◽  
The Impact

Abstract Different methodologies have been developed throughout the years to identify environmental microorganisms to improve bioremediation techniques, determine susceptibility profiles of bacteria in contaminated environments, and reduce the impact of microorganisms in ecosystems. Two methods of bacterial biochemical identification are compared and the susceptibility profile of bacteria, isolated from residential and industrial wastewater, is determined. Twenty-four bacteria were retrieved from the bacteria bank of the Environmental Microbiology Laboratory at the Institute of Biology (IB) of the Universidade Federal de Pelotas, Pelotas, Brazil. Bacteria were identified by conventional biochemical tests and by the VITEK ®2 automated system. Further, the susceptibility profile to antibiotics was also determined by the automated system. Six species of bacteria (Raoutella planticola, K. pneumoniae ssp. pneumoniae , Serratia marcescens, Raoutella sp., E. cloacae and Klebsiella oxytoca) were identified by conventional biochemical tests, while three species of bacteria (K. pneumoniae ssp. pneumoniae, S. marcescens and K. oxytoca ) were identified by VITEK®2 automated system. VITEK ®2 indicated agreement in 19 (79.17%) isolates and difference in five (20.83%) isolates when compared to results from conventional biochemical tests. Further, antibiotic susceptibility profile results showed that all isolates (100%) were resistant to at least one out of the 18 antibiotics tested by VITEK®2. Thus, no multi-resistant bacteria that may be used in effluent treatment systems or in bioremediation processes have been reported. Results indicate VITEK ® 2 automated system as a potential methodology in the determination of susceptibility profile and identification of environmental bacteria.

scholarly journals Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco

Food Control ◽  
2018 ◽  
Vol 84 ◽  
pp. 436-441 ◽  
Author(s):  
Nadia Amajoud ◽  
Alexandre Leclercq ◽  
Jose M. Soriano ◽  
Hélène Bracq-Dieye ◽  
Mohammed El Maadoudi ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Products

scholarly journals Distribution and antibiotic susceptibility profiles of Aeromonas spp. from different aquatic environments in north Lebanon

2018 ◽  
Vol 12 (02.1) ◽  
pp. 6S
Author(s):  
Rayane Rafei ◽  
Imad Al Kassaa ◽  
Marwan Osman ◽  
Fouad Dabboussi ◽  
Monzer Hamze
Keyword(s):  
Multiplex Pcr ◽  
Water Samples ◽  
Antibiotic Susceptibility ◽  
Aquatic Environments ◽  
Beta Lactams ◽  
Culture Methods ◽  
Resistant Strains ◽  
Seawater Samples ◽  
Susceptibility Profiles ◽  
Different Sources

Introduction: Bacteria of Aeromonas genus are ubiquitous organisms in aquatic environments. This work aims to explore the presence of four clinically relevant species in Lebanese waters and to study their susceptibility to antibiotics. Methods: One hundred water samples are collected in northern Lebanon from different sources (river, springs, wells, ponds, sea, chlorinated water and sewage) and analyzed by culture methods. The isolates were identified at genus level by a PCR assay targeting gcat gene. The presence of four species (A. hydrophila, A. caviae, A. media and A. veronii) was investigated by a multiplex PCR. The antibiotic susceptibility was also studied. Results: Aeromonas spp. was detected in 28% of investigated samples. In total, 38 isolates were identified as Aeromonas spp (10 out of 7 river water samples, 9 out of 35 spring, 9 among 26 wells, 5 out of 4 sewage samples, 2 out of 5 ponds, 2 out of 8 seawater samples and 1 out of 15 chlorinated water samples). Aeromonas hydrophila was the predominant species (19 isolates; 50%). The 3 other species were found to a lesser extent: A. caviae (3 isolates; 7.9%), A. veronii (2 isolates; 5.3%) and A. media (2 isolates; 5.3%). 12 other isolates (31.5%) remained unidentified by the used multiplex PCR technique. All isolates were resistant to more than one antibiotic. The most common resistance concerned beta-lactams. Conclusion: This study has highlighted the interesting distribution of these species in aquatic biotopes in Lebanon and the threat of potential transmission of these resistant strains to humans.

scholarly journals Prevalence and antibiotic susceptibility pattern of Klebsiella species isolated from various clinical samples in a tertiary care hospital Coimbatore

2021 ◽  
Vol 8 (10) ◽  
pp. 4886
Author(s):  
Vijayashree V. ◽  
Saikeerthana D. ◽  
Prabha P.
Keyword(s):  
Antibiotic Susceptibility ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Clinical Samples ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Klebsiella Species ◽  
Antibiotic Susceptibility Pattern ◽  
Sensitivity Pattern

Background: The genus Klebsiella of Enterobacteriaceae family is ubiquitous in nature. They cause many nosocomial infections like pneumonia, urinary tract infections, wound infections, bacteremia and septicemia. Multidrug resistance is seen in Klebsiella which serves as the most common cause of increased morbidity and mortality. This study reveals the prevalence and antibiotic sensitivity pattern of Klebsiella species from various clinical samples. The primary objectives are as follows: To isolate and characterize Klebsiella species from various clinical samples. To study the antibiotic susceptibility pattern of Klebsiella isolates.Methods: This prospective study was conducted in our tertiary care hospital during the period from August 2019 to October 2019. A total of 3521 samples were tested during this period. The samples include blood, sputum, urine, and pus.Results: Out of the total samples tested,1106 samples were showing the growth of the organisms. Among this,351 were identified as Klebsiella species and the highest rate of isolation of Klebsiella species is from the sputum sample and also the same was reported high in medical wards. The study also shows that the isolation of Klebsiella species shows male preponderance when compared to females. The antibiotic sensitivity pattern was done by Kirby-Bauer's disc diffusion method and the sensitivity was noted to be higher to amikacin and ciprofloxacin.Conclusions: Thus, this study shows the prevalence rate of Klebsiella species and sensitivity pattern of Klebsiella, which may help select appropriate antibiotics and prevent overuse and misuse of antibiotics.

scholarly journals Antibiotic susceptibility profiles of Mycoplasma sp. 1220 strains isolated from geese in Hungary

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Dénes Grózner ◽  
Zsuzsa Kreizinger ◽  
Kinga M. Sulyok ◽  
Zsuzsanna Rónai ◽  
Veronika Hrivnák ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Profiles
Sign in / Sign up

Export Citation Format

Share Document