A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress

AbstractMung bean CYP90A2 is a putative brassinosteroid (BR) synthetic gene that shares 77% identity with the Arabidopsis CPD gene. It was strongly suppressed by chilling stress. This implies that exogenous treatment with BR could allow the plant to recover from the inhibited growth caused by chilling. In this study, we used proteomics to investigate whether the mung bean epicotyl can be regulated by brassinosteroids under conditions of chilling stress. Mung bean epicotyls whose growth was initially suppressed by chilling partly recovered their ability to elongate after treatment with 24-epibrassinolde; 17 proteins down-regulated by this chilling were re-up-regulated. These up-regulated proteins are involved in methionine assimilation, ATP synthesis, cell wall construction and the stress response. This is consistent with the re-up-regulation of methionine synthase and S-adenosyl-L-methionine synthetase, since chilling-inhibited mung bean epicotyl elongation could be partially recovered by exogenous treatment with DL-methionine. This is the first proteome established for the mung bean species. The regulatory relationship between brassinosteroids and chilling conditions was investigated, and possible mechanisms are discussed herein.

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Genome-wide identification and characterization of TALE superfamily genes in cotton reveals their functions in regulating secondary cell wall biosynthesis

BMC Plant Biology ◽

10.1186/s12870-019-2026-1 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 3

Author(s):

Qiang Ma ◽

Nuohan Wang ◽

Pengbo Hao ◽

Huiru Sun ◽

Congcong Wang ◽

...

Keyword(s):

Cell Wall ◽

Cotton Fiber ◽

Secondary Cell Wall ◽

Fiber Strength ◽

Fiber Quality ◽

Expression Patterns ◽

Evolutionary Analysis ◽

Regulatory Relationship ◽

Genome Wide ◽

Regulation Functions

Abstract Background Cotton fiber length and strength are both key traits of fiber quality, and fiber strength (FS) is tightly correlated with secondary cell wall (SCW) biosynthesis. The three-amino-acid-loop-extension (TALE) superclass homeoproteins are involved in regulating diverse biological processes in plants, and some TALE members has been identified to play a key role in regulating SCW formation. However, little is known about the functions of TALE members in cotton (Gossypium spp.). Results In the present study, based on gene homology, 46, 47, 88 and 94 TALE superfamily genes were identified in G. arboreum, G. raimondii, G. barbadense and G. hirsutum, respectively. Phylogenetic and evolutionary analysis showed the evolutionary conservation of two cotton TALE families (including BEL1-like and KNOX families). Gene structure analysis also indicated the conservation of GhTALE members under selection. The analysis of promoter cis-elements and expression patterns suggested potential transcriptional regulation functions in fiber SCW biosynthesis and responses to some phytohormones for GhTALE proteins. Genome-wide analysis of colocalization of TALE transcription factors with SCW-related QTLs revealed that some BEL1-like genes and KNAT7 homologs may participate in the regulation of cotton fiber strength formation. Overexpression of GhKNAT7-A03 and GhBLH6-A13 significantly inhibited the synthesis of lignocellulose in interfascicular fibers of Arabidopsis. Yeast two-hybrid (Y2H) experiments showed extensive heteromeric interactions between GhKNAT7 homologs and some GhBEL1-like proteins. Yeast one-hybrid (Y1H) experiments identified the upstream GhMYB46 binding sites in the promoter region of GhTALE members and defined the downstream genes that can be directly bound and regulated by GhTALE heterodimers. Conclusion We comprehensively identified TALE superfamily genes in cotton. Some GhTALE members are predominantly expressed during the cotton fiber SCW thicking stage, and may genetically correlated with the formation of FS. Class II KNOX member GhKNAT7 can interact with some GhBEL1-like members to form the heterodimers to regulate the downstream targets, and this regulatory relationship is partially conserved with Arabidopsis. In summary, this study provides important clues for further elucidating the functions of TALE genes in regulating cotton growth and development, especially in the fiber SCW biosynthesis network, and it also contributes genetic resources to the improvement of cotton fiber quality.

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Plant cell wall construction crew

Science ◽

10.1126/science.369.6507.1069-b ◽

2020 ◽

Vol 369 (6507) ◽

pp. 1069.2-1069

Author(s):

Michael A. Funk

Keyword(s):

Cell Wall ◽

Plant Cell ◽

Plant Cell Wall ◽

Wall Construction

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Revisiting Old Questions and New Approaches to Investigate the Fungal Cell Wall Construction

Current Topics in Microbiology and Immunology - The Fungal Cell Wall ◽

10.1007/82_2020_209 ◽

2020 ◽

pp. 331-369

Author(s):

Michael Blatzer ◽

Anne Beauvais ◽

Bernard Henrissat ◽

Jean-Paul Latgé

Keyword(s):

Cell Wall ◽

Fungal Cell Wall ◽

Fungal Cell ◽

New Approaches ◽

Wall Construction

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Interactions of proteins with other wall components: a pivotal step in fungal cell wall construction

Canadian Journal of Botany ◽

10.1139/b95-273 ◽

1995 ◽

Vol 73 (S1) ◽

pp. 384-387 ◽

Cited By ~ 3

Author(s):

R. Sentandreu ◽

M. Sentandreu ◽

M. V. Elorza ◽

M. Iranzo ◽

S. Mormeneo

Keyword(s):

Cell Wall ◽

Protein Interactions ◽

Cell Walls ◽

Noncovalent Interactions ◽

Fungal Cell Wall ◽

Fungal Cell ◽

Covalent Bonds ◽

Viscoelastic Composite ◽

Wall Construction ◽

Wall Components

Following synthesis of its individual components, the cell wall of Candida albicans is assembled extracellularly in two steps. First, a viscoelastic composite is formed by noncovalent interactions between mannoproteins and other wall components. Second, the initial network is consolidated by formation of covalent cross-linkages among the wall polymers. In both processes, specific proteins may regulate the final yeast or mycelial morphology. These proteins might carry out part of what could be called a morphogenetic code. Experimental results have shown that some mannoproteins form supramolecular complexes. They are secreted independently, but released together from cell walls by hydrolases. In C. albicans cell walls a transglutaminase activity has been detected that could be responsible for the formation of covalent bonds between structural proteins. Key words: fungal cell wall, construction, morphogenesis, protein interactions, noncovalent linkages, covalent linkages.

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Relationships between cell-wall β-1,3-endoglucanase activity and auxin-induced elongation in mung bean hypocotyl segments

PROTOPLASMA ◽

10.1007/bf02539805 ◽

1997 ◽

Vol 199 (1-2) ◽

pp. 49-56 ◽

Cited By ~ 6

Author(s):

S. Mutaftschiev ◽

R. Prat ◽

M. Pierron ◽

G. Devilliers ◽

R. Goldberg

Keyword(s):

Cell Wall ◽

Mung Bean ◽

Endoglucanase Activity ◽

Bean Hypocotyl ◽

Mung Bean Hypocotyl ◽

Hypocotyl Segments

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Antioxidant Activity of Cell Wall Polysaccharides in Mung Bean Sprout Hypocotyls

Nippon Shokuhin Kagaku Kogaku Kaishi ◽

10.3136/nskkk.54.247 ◽

2007 ◽

Vol 54 (6) ◽

pp. 247-252 ◽

Cited By ~ 3

Author(s):

Isao Hayami ◽

Yoshie Motomura ◽

Takashi Nishizawa

Keyword(s):

Antioxidant Activity ◽

Cell Wall ◽

Mung Bean ◽

Cell Wall Polysaccharides ◽

Bean Sprout

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Relationship between salicylic acid and resistance to mite in strawberry

Folia Horticulturae ◽

10.2478/fhort-2021-0008 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Juliano T. Vilela de Resende ◽

Rafael Matos ◽

Douglas M. Zeffa ◽

Leonel Vinicius Constantino ◽

Silas M. Alves ◽

...

Keyword(s):

Cell Wall ◽

Salicylic Acid ◽

Glandular Trichomes ◽

Parenchyma Cell ◽

Anatomical Changes ◽

Induce Resistance ◽

Cultivated Species ◽

Choice Assay ◽

Two Spotted Spider Mite ◽

Exogenous Treatment

Abstract The two-spotted spider mite (TSSM) Tetranychus urticae is a polyphagous pest which infests several wild and cultivated species of plants worldwide. To date, this mite is the most deleterious pest attacking the strawberry plant under a protected environment. Exogenously applied salicylic acid (SA) has been found to induce resistance to biotic and abiotic stresses. Morpho-anatomical changes in strawberry leaflets and TSSM preference tests were investigated in response to SA treatment. Plants grown in a greenhouse were foliar sprayed with different concentrations of SA (0 mg · L−1, 25 mg · L−1, 50 mg · L−1, 75 mg · L−1 and 100 mg · L−1). After the third application, certain parameters including the number of glandular and non-glandular trichomes, thickness of leaflet, abaxial cell wall, adaxial cell wall, palisade and lacunous parenchyma and mesophyll were measured using scanning electronic microscopy (SEM). Two-choice and non-choice assays were employed to verify the TSSM preference. Exogenous treatment with SA promoted morpho-anatomical changes in the following parameters, namely: thickness of the leaflets, mesophyll, lacunous and palisade parenchyma, cell wall (abaxial and adaxial) and the number of glandular and non-glandular trichomes in strawberry leaflets. In general, TSSM preferred less leaflets treated with SA compared with the control in a two-choice assay. A lesser number of TSSM eggs and live females were also recorded in leaflets treated with SA compared with the control plants in no-choice assays. The values of the number of eggs and live females correlated negatively with those obtained for the morpho-anatomical traits induced by exogenous SA.

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A role for the MAP kinase gene MKC1 in cell wall construction and morphological transitions in Candida albicans

Microbiology ◽

10.1099/00221287-144-2-411 ◽

1998 ◽

Vol 144 (2) ◽

pp. 411-424 ◽

Cited By ~ 102

Author(s):

F. Navarro-Garcia ◽

R. Alonso-Monge ◽

H. Rico ◽

J. Pla ◽

R. Sentandreu ◽

...

Keyword(s):

Cell Wall ◽

Candida Albicans ◽

Map Kinase ◽

Kinase Gene ◽

Morphological Transitions ◽

Wall Construction

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Direct visualization of cross-links in the primary plant cell wall

Journal of Cell Science ◽

10.1242/jcs.96.2.323 ◽

1990 ◽

Vol 96 (2) ◽

pp. 323-334 ◽

Cited By ~ 1

Author(s):

M. C. MCCANN ◽

B. WELLS ◽

K. ROBERTS

Keyword(s):

Cell Wall ◽

Plant Cell Wall ◽

Three Dimensional ◽

Preliminary Evidence ◽

Cellulose Microfibrils ◽

Primary Cell Wall ◽

Positive Role ◽

Extraction Step ◽

Cross Links ◽

Wall Construction

We have investigated the structure of the onion primary cell wall at high resolution, using shadowed replicas of rapidly frozen deep-etched specimens. We have sequentially extracted polymers from the wall and have visualized both these and the remaining structures at each extraction step. By viewing the structures in as near their native state as possible, an accurate three-dimensional picture of wall construction has been assembled, facilitated by viewing stereo pairs of micrographs. Our observations show that the physical links between cellulose microfibrils that we observe in the intact wall are generally shorter (20–40 nm) than the isolated molecules we extract (30->700nm), suggesting that lateral interactions must occur between linking polymers and cellulose in muro. These cross-links are hemicellulosic and we believe them to be xyloglucans: their removal allows increased lateral association of microfibrils. Na2CO3-extractable pectic fractions form a separate coextensive network, the removal of which does not affect basic cellulose/ hemicellulose architecture. Preliminary evidence for a lamellate model of wall construction has been obtained. In addition, we propose a positive role for hemicellulose in maintaining the ordered spacing of cellulose micronbrils, perhaps regulating wall porosity and strength. The basic wall parameters that we derive impose constraints on possible cell wall models.

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