scholarly journals Studies on genetic changes in rye samples (Secale cereale L.) maintained in a seed bank

2006 ◽  
Vol 11 (3) ◽  
Author(s):  
Katarzyna Chwedorzewska ◽  
Piotr Bednarek ◽  
Renata Lewandowska ◽  
Paweł Krajewski ◽  
Jerzy Puchalski
Keyword(s):  
Genomic Dna ◽  
Fragment Length Polymorphism ◽  
Gene Bank ◽  
Genetic Changes ◽  
Long Term Storage ◽  
Seed Ageing ◽  
Secale Cereale L ◽  
Principle Coordinate Analysis ◽  
Term Storage

AbstractThe aim of this study was to identify genetic changes in rye seeds induced by natural ageing during long-term storage and consecutive regeneration cycles under gene bank conditions. Genomic DNA from four rye samples varying in their initial viability after one and three cycles of reproduction was analyzed by AFLP (amplified fragment length polymorphism) fingerprinting. Seven EcoRI/MseI primer combinations defined 663 fragments, and seven PstI/MseI primer combinations defined 551 fragments. The variation in the frequency of the seventy-four EcoRI/MseI bands was statistically significant between samples. These changes could be attributed to genetic changes occurring during storage and regeneration. However, the PstI/MseI fragments appeared to be uninfluenced by seed ageing, regeneration and propagation. A combined Principle Coordinate Analysis revealed differences between samples with different initial viability. We showed that materials with low initial viability differ in their response from highly viable ones, and that the changes exhibited in the former case are preserved through regeneration cycles.

scholarly journals Capture and Purification of Plant Genomic DNA on a Readily-available Cellulose Matrix

2017 ◽  
Author(s):  
Megan M. Thompson ◽  
Estelle M. Hrabak
Keyword(s):  
Arabidopsis Thaliana ◽  
Nucleic Acids ◽  
Plant Species ◽  
Efficient Method ◽  
Genomic Dna ◽  
Low Cost ◽  
Long Term Storage ◽  
Cellulose Matrix ◽  
Term Storage

AbstractWhatman FTA ®Cards are a fast and efficient method for capturing and storing nucleic acids but are cost-prohibitive for some researchers. We developed a method that substitutes readily-available cellulose-based paper and homemade washing buffer for commercial FTA ®Cards and FTA ®Purification Reagent. This method is suitable for long-term storage of DNA from many plant species, including Arabidopsis thaliana, prior to purification and PCR.Method SummaryHere we report a low-cost method for long-term storage of plant genomic DNA on a readily available cellulose matrix.

scholarly journals Evaluarea potenţialului de păstrare a seminţelor mostrelor din colecţia de Triticum durum L.

2021 ◽  
Author(s):  
Lolita Melian ◽  
◽  
Liudmila Corlateanu ◽  
Victoria Mihailă ◽  
Doina Cutitaru ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Triticum Durum ◽  
Accelerated Aging ◽  
Gene Bank ◽  
Stress Factors ◽  
Long Term Storage ◽  
Dry Biomass ◽  
Seedling Roots ◽  
Term Storage

The test of accelerated aging of seeds (AAS) was applied on 7 genotypes of durum wheat, and the morphophysiological parameters of seeds and seedlings, such as dynamics of seed germination, length of seedling roots, fresh and dry biomass of seedlings, were investigated. The genotypic features of collection samples of durum wheat were identified after exposure to stress factors (high temperature and humidity). Such a grouping of genotypes according to their potential ability to preserve the viability of seeds is an important complex characteristic of collection samples when they are placed for long-term storage in a plant gene bank.

scholarly journals A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment

2020 ◽  
Vol 50 (4) ◽  
pp. 335-338
Author(s):  
Érica MANGARAVITE ◽  
Vanessa TERRA ◽  
Eric Koiti Okiyama HATTORI ◽  
Thaís Carolina da Silva DAL’SASSO ◽  
Leonardo Lopes BHERING ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Storage Conditions ◽  
Microsatellite Genotyping ◽  
Short Term ◽  
Feasible Method ◽  
Long Term Storage ◽  
Canopy Trees ◽  
Amazonian Forests ◽  
Term Storage

ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapted a feasible methodology for extracting genomic DNA from cambium tissue harvested in the field for the assessment with PCR. We tested three storage conditions (two buffers and a silica gel) and four periods of time after harvest. We used previously described protocols and tested them on three species that occur in Amazonian forests and other biomes: Anadenanthera peregrina var. peregrina, Cedrela fissilis, and Ceiba speciosa. Our protocol obtained suitable PCR-grade genomic DNA for DNA sequencing and microsatellite genotyping. We recommend the use of silica for long-term storage and the buffer with ascorbic acid for short-term storage.

scholarly journals The Integrity and Yield of Genomic DNA Isolated from Whole Blood Following Long-Term Storage at −30°C

2018 ◽  
Vol 16 (2) ◽  
pp. 106-113 ◽  
Author(s):  
Wenlong Carl Chen ◽  
Robyn Kerr ◽  
Andrew May ◽  
Babongile Ndlovu ◽  
Anelisa Sobalisa ◽  
...  
Keyword(s):  
Whole Blood ◽  
Genomic Dna ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Appearance of Heterosis in Onion Hybrids Obtained on Sterile Basis Applicated to Conditions of Azerbaijan

2020 ◽  
pp. 12-18
Author(s):  
Sabir Hasanov ◽  
◽  
Chimnaz Namazova ◽  
Zumrud Abbaszadeh ◽  
◽  
...  
Keyword(s):  
Life Quality ◽  
Source Material ◽  
Gene Bank ◽  
F1 Hybrids ◽  
Early Maturation ◽  
Long Term Storage ◽  
Allium Cepa L ◽  
Almost All ◽  
Term Storage

The main goal of the study was to obtain hybrids having a heterositic effect using sterile lines of onions (Allium cepa L.) applicated to conditions of Azerbaijan. We used to study a valuable source material from the collection of the National Gene Bank of Azerbaijan. As a result of the combining ability evaluation of the sterile line and the pollinator, the most promising combinations (Arzamas S-1 × Hachmaz Mest, Luganskiy S-2 × Dusti, Arzamas S-2 × Ordubad-2, Luganskiy S-1 × Kaba, Arzamas S-2 × Sabir) were revealed the greatest heterosis efficiency of yield and shelf life quality were selected. The yield of hybrids obtained with the participation of varieties of Arzamas S-1 and Arzamas S-2 and paternal varieties, compared with the Strigunovsky variety, is 17.0–78.0 % higher on average for all the years of testing. The study of the keeping capacity of F1 hybrids showed that almost all of them have a higher keeping capacity than the original varieties. The keeping capacity of hybrids especially increases, the initial varieties of which do not have long-term storage sustainability. So, bulbs of varieties Dustu, Peshpazak, Sabir, Khachmaz mestniy, Ordubad-1 for 180-200 days of storage remained only 26-29 %. Hybrids, obtained with the participation of these varieties; by the end of the study period, the keeping capacity remained at 49.8-88.7 %, i.e. 1.9-3.1 times better. Many F1 hybrids perform heterosis and early maturation, the combinations Arzamas S-2 × Strigunovsky, Arzamas S-2 × Dusti, Arzamas S-1 × Strigunovsky, Lugansky S-2 × Vostochny, Lugansky S-2 × Dusti, Arzamas S-1 × Khachmaz mestniy, Luganskiy S-1 × Kabo were more early matured than the standard for 14-18 days.

scholarly journals The Effect of Long-Term Storage on Mycobacterium bovis

2021 ◽  
Vol 70 (3) ◽  
pp. 327-337
Author(s):  
OLEXIY TKACHENKO ◽  
NATALI KOZAK ◽  
MARYNA BILAN ◽  
VOLODYMYR HLEBENIUK ◽  
NATALIA ALEKSEEVA ◽  
...  
Keyword(s):  
Low Temperature ◽  
Mycobacterium Bovis ◽  
Nutrient Medium ◽  
Tween 80 ◽  
Initial Strain ◽  
Genetic Changes ◽  
Long Term Storage ◽  
Solid Nutrient Medium ◽  
Term Storage

It was established that when stored for many years (10–13 years) in low-temperature conditions (3°C), without sub-culture on a nutrient medium, Mycobacterium bovis grew as visible colonies along the line of inoculation. However, due to long-term storage in conditions of low temperature (3°C) morphology of mycobacteria differed significantly from initial cultures formed by rod-shaped bacteria. Some of them became pigment-forming and smooth on the surface. Unlike the initial strain of mycobacteria, a perennial bacteria stored under hard conditions did not cause the death of guinea pigs or their sensitization to a purified protein derivative for mammals. Morphological forms of the perennial mycobacteria had the following changes: pigment forming, L-forms of the vesicular type, non-acid-fast thread-like (filamentous) bacillary forms, and elementary bodies when compared to the initial strain. There were also some genetic changes in the target DNA due to the long-term storage of M. bovis. It may indicate a mutation in the pathogen’s DNA. These mycobacteria had altered biochemical activity during storage. The number of passages on the solid nutrient medium did not affect their fermentative activity. However, the low cultivation temperature increases mycobacterial catalase activity and the ability to hydrolyze Tween-80.

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Sign in / Sign up

Export Citation Format

Share Document