Ultrastructural and Molecular Identification of the sarcocysts of Sarcocystis tenella and Sarcocystis arieticanis Infecting Domestic Sheep (Ovis aries) from Egypt

2019 ◽  
Vol 64 (3) ◽  
pp. 501-513
Author(s):  
Ahmed El-Morsey ◽  
Walied Abdo ◽  
Khaled Sultan ◽  
Nagwa M. Elhawary ◽  
Attia Abdallah AbouZaid
Keyword(s):  
Molecular Identification ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Sarcocystis Tenella

​Re-sequencing of Whole Genome Jordanian Awassi Ewes using Hiseq Sequencing Technology: Major Step Towards Sheep Genomic Selection

2021 ◽  
Author(s):  
Hussein Migdadi ◽  
Nizar Haddad ◽  
Ruba AlOmari ◽  
Mohammad Brake ◽  
Mustafa AlShdaifat ◽  
...  
Keyword(s):  
Production Systems ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Major Step ◽  
Illumina Hiseq ◽  
Structure Variation ◽  
Awassi Sheep ◽  
Wide Range ◽  
Number Variation ◽  
Paired End Sequencing

Background: Jordanian Awassi sheep (Ovis aries) is the dominant fat tail sheep breed that appeals to customers because of its various production systems, including fiber, meat and milk. This report is the first whole ewe genome sequence (WGS) of O. aries submitted in the NCBI database from Jordan. Methods: 64 Paired-end sequencing libraries were constructed and subjected to Illumina Hiseq 2500 sequencing system. High-quality reads were aligned against the reference sheep genome and detecting comprehensive sources (SNPs, InDels, SV, CNVs) of genetic variations. We have deposited data sequences at the NCBI under SRA (sequence reads archives) under the accession numbers SRR11128863, PRJNA574879. Result: Genome resequencing of Jordanian Awassi ewe was carried out with approximately 93.88 Gb with a mapping rate and effective mapping depths were 99.28% and 36.32. Around 19 million SNPs, 3,6 million InDels, 35,180 Structure variation and 13,524 copy number variation among the Jordanian ewe genome were detected. This wide range of genetic variation provides a framework for further genetic studies that will help understand the molecular basis underlying phenotypic variation of economically important traits in sheep and improve intrinsic defects in domestic sheep breeds.

Field metabolic rate and water turnover of red kangaroos and sheep in an arid rangeland: an empirically derived dry-sheep-equivalent for kangaroos

2009 ◽  
Vol 57 (1) ◽  
pp. 23 ◽  
Author(s):  
A. J. Munn ◽  
T. J. Dawson ◽  
S. R. McLeod ◽  
D. B. Croft ◽  
M. B. Thompson ◽  
...  
Keyword(s):  
Metabolic Rate ◽  
Grazing Pressure ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Detailed Knowledge ◽  
Water Turnover ◽  
Field Metabolic Rate ◽  
Red Kangaroo ◽  
Resource Requirements ◽  
Comparative Measure

Sustainable management of pastures requires detailed knowledge of total grazing pressure, but this information is critically lacking in Australia’s rangelands where livestock co-occur with large herbivorous marsupials. We present the first comparative measure of the field metabolic rate (an index of food requirement) of Australia’s largest marsupial, the red kangaroo (Macropus rufus), with that of domestic sheep (Ovis aries; merino breed). We tested the assumption that the grazing pressure of red kangaroos is equivalent to 0.7 sheep, and show this to be a two-fold overestimation of their contribution to total grazing. Moreover, kangaroos had extraordinarily lower rates of water turnover, being only 13% that of sheep. Consequently, our data support arguments that the removal of kangaroos may not markedly improve rangeland capacity for domestic stock. Furthermore, given the low resource requirements of kangaroos, their use in consumptive and non-consumptive enterprises can provide additional benefits for Australia’s rangelands than may occur under traditional rangeland practices.

Description of Diploplastron dehorityi sp. nov. (Entodiniomorphida, Ophryoscolecidae), a new rumen ciliate from Brazilian sheep (Ovis aries)

Zootaxa ◽  
2017 ◽  
Vol 4258 (6) ◽  
pp. 581 ◽  
Author(s):  
FRANCIANE CEDROLA ◽  
ROBERTO JÚNIO PEDROSO DIAS ◽  
ISABEL MARTINELE ◽  
MARTA D’AGOSTO
Keyword(s):  
New Species ◽  
Body Shape ◽  
The Body ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Diagnostic Features ◽  
Ciliate Community ◽  
The Right ◽  
A New Species

To date the genus Diploplastron comprised only one species of ophryoscolecid ciliate, Diploplastron affine, which is characterized by having two retractable ciliary zones in the anterior end of the body, two slender and juxtaposed skeletal plates on the right side, a rod shape macronucleus, and two contractile vacuoles. During study on the characterization of rumen ciliate community composition in Brazilian domestic sheep, we observed ciliates with atypical morphology but with diagnostic features of genus Diploplastron. This study describes Diploplastron dehorityi, a new species of ophryoscolecid ciliate, that differs from D. affine, primarily, in the morphology of skeletal plates, morphology of nuclear apparatus and body shape. In addition to the similarities between the new species and congener species, D. dehorityi has some morphological similarities to species of genus Eremoplastron.

scholarly journals Differences in leukocyte differentiation molecule abundances on domestic sheep (Ovis aries) and bighorn sheep (Ovis canadensis) neutrophils identified by flow cytometry

2016 ◽  
Vol 46 ◽  
pp. 40-46 ◽  
Author(s):  
Margaret A. Highland ◽  
David A. Schneider ◽  
Stephen N. White ◽  
Sally A. Madsen-Bouterse ◽  
Donald P. Knowles ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Bighorn Sheep ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Domestic Sheep

scholarly journals Comparison of Passively Transferred Antibodies in Bighorn and Domestic Lambs Reveals One Factor in Differential Susceptibility of These Species to Mannheimia haemolytica-Induced Pneumonia

2011 ◽  
Vol 18 (7) ◽  
pp. 1133-1138 ◽  
Author(s):  
Caroline N. Herndon ◽  
Sudarvili Shanthalingam ◽  
Donald P. Knowles ◽  
Douglas R. Call ◽  
Subramaniam Srikumaran
Keyword(s):  
Differential Susceptibility ◽  
Critical Factor ◽  
Surface Antigens ◽  
Ovis Canadensis ◽  
Ovis Aries ◽  
Mannheimia Haemolytica ◽  
Domestic Sheep ◽  
Experimental Conditions ◽  
Content Type ◽  
Impaired Ability

ABSTRACTMannheimia haemolyticaconsistently causes fatal bronchopneumonia in bighorn sheep (BHS;Ovis canadensis) under natural and experimental conditions. Leukotoxin is the primary virulence factor of this organism. BHS are more susceptible to developing fatal pneumonia than the related speciesOvis aries(domestic sheep [DS]). In BHS herds affected by pneumonia, lamb recruitment is severely impaired for years subsequent to an outbreak. We hypothesized that a lack of maternally derived antibodies (Abs) againstM. haemolyticaprovides an immunologic basis for enhanced susceptibility of BH lambs to population-limiting pneumonia. Therefore, the objective of this study was to determine the titers of Abs directed againstM. haemolyticain the sera of BH and domestic lambs at birth through 12 weeks of age. Results revealed that BH lambs had approximately 18-fold lower titers of Ab against surface antigens ofM. haemolyticaand approximately 20-fold lower titers of leukotoxin-neutralizing Abs than domestic lambs. The titers of leukotoxin-neutralizing Abs in the serum and colostrum samples of BH ewes were approximately 157- and 50-fold lower than those for domestic ewes, respectively. Comparatively, the higher titers of parainfluenza 3 virus-neutralizing Abs in the BH lambs ruled out the possibility that these BHS had an impaired ability to passively transfer Abs to their lambs. These results suggest that lower levels of leukotoxin-neutralizing Abs in the sera of BH ewes, and resultant low Ab titers in their lambs, may be a critical factor in the poor lamb recruitment in herds affected by pneumonia.

scholarly journals Impact Protection Potential of Mammalian Hair: Testing the Pugilism Hypothesis for the Evolution of Human Facial Hair

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
E A Beseris ◽  
S E Naleway ◽  
D R Carrier
Keyword(s):  
Interpersonal Violence ◽  
Epoxy Composite ◽  
Homo Sapiens ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Contest Competition ◽  
Time Data ◽  
Facial Skeleton ◽  
Tissue Samples ◽  
Facial Hair

Abstract Because facial hair is one of the most sexually dimorphic features of humans (Homo sapiens) and is often perceived as an indicator of masculinity and social dominance, human facial hair has been suggested to play a role in male contest competition. Some authors have proposed that the beard may function similar to the long hair of a lion’s mane, serving to protect vital areas like the throat and jaw from lethal attacks. This is consistent with the observation that the mandible, which is superficially covered by the beard, is one of the most commonly fractured facial bones in interpersonal violence. We hypothesized that beards protect the skin and bones of the face when human males fight by absorbing and dispersing the energy of a blunt impact. We tested this hypothesis by measuring impact force and energy absorbed by a fiber epoxy composite, which served as a bone analog, when it was covered with skin that had thick hair (referred to here as “furred”) versus skin with no hair (referred to here as “sheared” and “plucked”). We covered the epoxy composite with segments of skin dissected from domestic sheep (Ovis aries), and used a drop weight impact tester affixed with a load cell to collect force versus time data. Tissue samples were prepared in three conditions: furred (n = 20), plucked (n = 20), and sheared (n = 20). We found that fully furred samples were capable of absorbing more energy than plucked and sheared samples. For example, peak force was 16% greater and total energy absorbed was 37% greater in the furred compared to the plucked samples. These differences were due in part to a longer time frame of force delivery in the furred samples. These data support the hypothesis that human beards protect vulnerable regions of the facial skeleton from damaging strikes.

69 Invitro production of ovine embryos using either fresh or frozen-thawed semen

2021 ◽  
Vol 33 (2) ◽  
pp. 141
Author(s):  
G. Márquez-Márquez ◽  
A. Velázquez-Roque ◽  
F. Villaseñor-González ◽  
M. Kjelland ◽  
H. Álvarez-Gallardo ◽  
...  
Keyword(s):  
Semen Quality ◽  
Small Ruminants ◽  
Physiological Saline ◽  
Ovis Aries ◽  
Penicillin G ◽  
Domestic Sheep ◽  
Assisted Reproductive Technique ◽  
Physiological Saline Solution ◽  
Α Level ◽  
Fresh Semen

Invitro embryo production (IVP) is an important tool for genetic improvement in small ruminants. Semen quality is one of the most important aspects to consider for the success of this assisted reproductive technique. With ovine IVP, it is a common practice to use fresh semen for IVF, but this could be a problem because the differences between ejaculates from the same animal are well documented and a source of variation in IVP results. The objective of this research was to compare the effect of fresh and frozen–thawed domestic sheep (Ovis aries) semen on IVF for ovine IVP. The research was carried out in the reproduction laboratory at the Palominos Ranch (Jalisco, México). The IVP was performed with a continuous invitro culture system. Ovaries (n=186) were collected from a slaughterhouse (León, México) and transported to the laboratory within 2h in physiological saline solution (0.9% NaCl) supplemented with penicillin G (100IU mL−1) and streptomycin sulphate (100µg mL−1). For IVP, IVF-Bioscience™ media were used for IVM, IVF, and invitro culture (IVC). For IVM, the cumulus–oocyte complexes (COCs) were selected (only grades 1 and 2) and matured for 24h at 38.5°C in 5% CO2 in air and 100% humidity. Matured oocytes (n=1000) were invitro fertilized using either fresh or frozen–thawed semen (Triladyl™; Minitube) from the same sheep, at a concentration of 2×106 sperm mL−1, for 18h in 38.5°C, 5% CO2 in air, and 100% humidity. The presumptive zygotes were denuded by pipetting and set in IVC until Day 7 at 38.5°C, 5% CO2, 5% O2, and 90% N2 at 100% humidity. The percentages of cleavage, embryos with more than 6 cells, and blastocysts on Day 7 of culture were evaluated, based on the initial number of oocytes entering into IVM. Statistical analyses were carried out with the GLM procedure of SAS software (version 9.3; SAS Institute Inc.) to evaluate the results of fresh versus frozen–thawed (α level=0.05). Rates of cleavage, embryos with more than 6 cells, and blastocysts on Day 7 were similar (P>0.05): fresh 52.3±3.0%, 43.6±2.6%, and 34.3±2.9%, respectively; frozen–thawed: 53.3%±3.0, 41.1±2.6%, and 33.3±2.9%, respectively. In conclusion, under the conditions of this research, the use of fresh and frozen–thawed semen had similar results for ovine IVP.

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