scholarly journals Detection of Toxocara canis larvae by PCR in the liver of experimentally infected Mongolian gerbils (Meriones unguiculatus)

2008 ◽  
Vol 45 (3) ◽  
pp. 147-149 ◽  
Author(s):  
A. Borecka ◽  
J. Gawor ◽  
M. Niedworok ◽  
B. Sordyl
Keyword(s):  
Clinical Manifestations ◽  
Toxocara Canis ◽  
Meriones Unguiculatus ◽  
Mongolian Gerbils ◽  
Igg Antibodies ◽  
Chain Reaction ◽  
Specific Igg ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Specific Igg Antibodies

AbstractToxocariasis is a common human zoonosis, which induces a clinically unapparent course of infection. Diagnosis is difficult and relies upon serological testing (searching of specific IgG antibodies by ELISA), laboratory abnormalities and clinical manifestations. The polymerase chain reaction (PCR) technique was adapted for the detection of Toxocara canis larvae in a host tissue. Mongolian gerbils (Meriones unguiculatus) were used as an animal model for human toxocariasis. 8 animals were inoculated with 1000 T. canis eggs, four uninfected were used as control. At 3, 5, 7, and 14 days post-infection, 2 infected and 1 control gerbil were killed and their livers were used for molecular analysis. Specific primer in the PCR reaction allowed identification of T. canis larvae, with the parasite gDNA found in the liver of all infected gerbils. The results indicate that the PCR method has a potential as a supporting technique for the diagnosis of human toxocariasis.

scholarly journals Characteristics of Ebola Virus Disease Survivor Blood and Semen in Liberia: Serology and Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2020 ◽  
Author(s):  
Aaron Kofman ◽  
Susanne Linderman ◽  
Kaihong Su ◽  
Lawrence J Purpura ◽  
Elizabeth Ervin ◽  
...  
Keyword(s):  
Ebola Virus ◽  
Virus Disease ◽  
Igg Antibodies ◽  
Chain Reaction ◽  
Male Survivors ◽  
Qrt Pcr ◽  
Specific Igg ◽  
Polymerase Chain ◽  
Study Participants ◽  
Specific Igg Antibodies

Abstract Introduction Ebola virus (EBOV), species Zaire ebolavirus, may persist in the semen of male survivors of Ebola virus disease (EVD). We conducted a study of male survivors of the 2014–2016 EVD outbreak in Liberia and evaluated their immune responses to EBOV. We report here findings from the serologic testing of blood for EBOV-specific antibodies, molecular testing for EBOV in blood and semen, and serologic testing of peripheral blood mononuclear cells (PBMCs) in a subset of study participants. Methods We tested for EBOV RNA in blood by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and for anti-EBOV-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies by enzyme-linked immunosorbent assay (ELISA) for 126 study participants. We performed PBMC analysis on a subgroup of 26 IgG-negative participants. Results All 126 participants tested negative for EBOV RNA in blood by qRT-PCR. The blood of 26 participants tested negative for EBOV-specific IgG antibodies by ELISA. PBMCs were collected from 23/26 EBOV IgG-negative participants. Of these, 1/23 participants had PBMCs that produced anti-EBOV-specific IgG antibodies upon stimulation with EBOV-specific glycoprotein (GP) and nucleoprotein (NP) antigens. Conclusions The blood of EVD survivors, collected when they did not have symptoms meeting the case definition for acute or relapsed EVD, is unlikely to pose a risk for EBOV transmission. We identified 1 IgM/IgG negative participant who had PBMCs that produced anti-EBOV-specific antibodies upon stimulation. Immunogenicity following acute EBOV infection may exist along a spectrum, and absence of antibody response should not be exclusionary in determining an individual’s status as a survivor of EVD.

scholarly journals Total serum IgE and parasite: specific IgG and IgA antibodies in human strongyloidiasis

1993 ◽  
Vol 35 (4) ◽  
pp. 361-365 ◽  
Author(s):  
Cláudio L. Rossi ◽  
Emilia E. H. Takahashi ◽  
Cláudia D. Partel ◽  
Lívia G.V.L. Teodoro ◽  
Luiz J. da Silva
Keyword(s):  
Elevated Serum ◽  
Clinical Manifestations ◽  
Case Series ◽  
Total Serum ◽  
Igg Antibodies ◽  
Serum Ige ◽  
Iga Antibodies ◽  
Specific Igg ◽  
Mean Concentration ◽  
Specific Igg Antibodies

Total serum IgE, and Strongyloides - specific IgG and IgA antibodies were studied in 27 patients with parasitologically proven strongyloidiasis. Clinical manifestations in this case series were investigated by a restrospective study of the patient's records. Total serum IgE levels were elevated (greater than 250 IU/ml) in 59% of the patients (mean concentration = 1364 IU/ml). Parasite - specific IgG and IgA antibodies were detected by ELISA in the serum of 23 (85.2%) and 21 (77.8%) patients, respectively. Elevated serum IgE and clinical manifestations were not useful indexes of the presence of strongyloidiasis. On the other hand, our results support the view that serologic tests, particularly ELISA for detecting Strongyloides - specific IgG antibodies, can be usefully exploited for diagnostic purposes in strongyloidiasis.

scholarly journals Contribution to canine babesiosis in the Czech Republic

2012 ◽  
Vol 81 (2) ◽  
pp. 91-95 ◽  
Author(s):  
Jarmila Konvalinová ◽  
Ivo Rudolf ◽  
Silvie Šikutová ◽  
Zdeněk Hubálek ◽  
Vlasta Svobodová ◽  
...  
Keyword(s):  
Czech Republic ◽  
Dermacentor Reticulatus ◽  
Igg Antibodies ◽  
Canine Babesiosis ◽  
The Czech Republic ◽  
Negative Results ◽  
Working Dogs ◽  
Specific Igg ◽  
Polymerase Chain ◽  
Specific Igg Antibodies

From March to November 2010, a total of 68 samples of blood from 41 hunting and working dogs that never left the Czech Republic were examined. Some dogs were sampled repeatedly. Blood samples were examined by polymerase chain reaction for the presence of DNA of piroplasms with negative results. Specific IgG antibodies against Babesia canis were detected by indirect immunofluorescence test, and five dogs (12.21%) were seropositive. Titres ranged from 50 to 200. One dog was positive in two samplings within 3 months. The highest number of positive samples was taken in June. The results of this study suggest a likely contact of the examined dogs with the parasite; although in 2005, a total of 340 adult unfed Dermacentor reticulatus ticks in 34 pools screened by PCR for babesiae were negative.

scholarly journals ToxocaraSeroprevalence in Patients with Idiopathic Parkinson's Disease: Chance Association or Coincidence?

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Tuncay Çelik ◽  
Yüksel Kaplan ◽  
Eser Ataş ◽  
Derya Öztuna ◽  
Said Berilgen
Keyword(s):  
Toxoplasma Gondii ◽  
Parkinson Disease ◽  
Causal Relation ◽  
Clinical History ◽  
Toxocara Canis ◽  
Igg Antibodies ◽  
History Of ◽  
Specific Igg ◽  
Genetic And Environmental Factors ◽  
Specific Igg Antibodies

Most cases of idiopathic Parkinson disease (IPD) are believed to be due to a combination of genetic and environmental factors. The purpose of this study is to investigate the relationship between toxocariasis and Parkinson disease (PD). Patients were selected from people who were admitted to the Movement Disorders Branch, Neurology Department of Elazığ University Faculty of Medicine Elazığ, Turkey. We studied specific IgG antibodies againstToxocara canis(T. canis) in 50 patients with idiopathic Parkinson and 50 healthy volunteers. We investigated the clinical history of three patients infected withT. canis. We also studied specific IgG antibodies againstToxoplasma gondiiin these groups. Antibodies anti-Toxocara caniswere found in 3 idiopathic PD (6%) (P=0.121) and antibody titer was not found in control. A patient had history of the presence of dog in current dog ownership. We did not detect any statistically significant association betweenT. canisand IPD. But, we believe that further comprehensive studies are required for understanding whether there is a causal relation between toxocariasis and PD. We didn’t find possible association betweenToxoplasma gondiiand IPD (P=0.617).

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