Comparison of Intrathecal Synthesis of Treponema pallidum-specific IgG Antibodies and Polymerase Chain Reaction for the Diagnosis of Neurosyphilis

1996 ◽  
Vol 283 (3) ◽  
pp. 295-305 ◽  
Author(s):  
Matthäus Moskophidis ◽  
Stefan Peters
Keyword(s):  
Polymerase Chain Reaction ◽  
Treponema Pallidum ◽  
Igg Antibodies ◽  
Chain Reaction ◽  
Intrathecal Synthesis ◽  
Specific Igg ◽  
Polymerase Chain ◽  
Specific Igg Antibodies

scholarly journals Characteristics of Ebola Virus Disease Survivor Blood and Semen in Liberia: Serology and Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2020 ◽  
Author(s):  
Aaron Kofman ◽  
Susanne Linderman ◽  
Kaihong Su ◽  
Lawrence J Purpura ◽  
Elizabeth Ervin ◽  
...  
Keyword(s):  
Ebola Virus ◽  
Virus Disease ◽  
Igg Antibodies ◽  
Chain Reaction ◽  
Male Survivors ◽  
Qrt Pcr ◽  
Specific Igg ◽  
Polymerase Chain ◽  
Study Participants ◽  
Specific Igg Antibodies

Abstract Introduction Ebola virus (EBOV), species Zaire ebolavirus, may persist in the semen of male survivors of Ebola virus disease (EVD). We conducted a study of male survivors of the 2014–2016 EVD outbreak in Liberia and evaluated their immune responses to EBOV. We report here findings from the serologic testing of blood for EBOV-specific antibodies, molecular testing for EBOV in blood and semen, and serologic testing of peripheral blood mononuclear cells (PBMCs) in a subset of study participants. Methods We tested for EBOV RNA in blood by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and for anti-EBOV-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies by enzyme-linked immunosorbent assay (ELISA) for 126 study participants. We performed PBMC analysis on a subgroup of 26 IgG-negative participants. Results All 126 participants tested negative for EBOV RNA in blood by qRT-PCR. The blood of 26 participants tested negative for EBOV-specific IgG antibodies by ELISA. PBMCs were collected from 23/26 EBOV IgG-negative participants. Of these, 1/23 participants had PBMCs that produced anti-EBOV-specific IgG antibodies upon stimulation with EBOV-specific glycoprotein (GP) and nucleoprotein (NP) antigens. Conclusions The blood of EVD survivors, collected when they did not have symptoms meeting the case definition for acute or relapsed EVD, is unlikely to pose a risk for EBOV transmission. We identified 1 IgM/IgG negative participant who had PBMCs that produced anti-EBOV-specific antibodies upon stimulation. Immunogenicity following acute EBOV infection may exist along a spectrum, and absence of antibody response should not be exclusionary in determining an individual’s status as a survivor of EVD.

scholarly journals Detection of Toxocara canis larvae by PCR in the liver of experimentally infected Mongolian gerbils (Meriones unguiculatus)

2008 ◽  
Vol 45 (3) ◽  
pp. 147-149 ◽  
Author(s):  
A. Borecka ◽  
J. Gawor ◽  
M. Niedworok ◽  
B. Sordyl
Keyword(s):  
Clinical Manifestations ◽  
Toxocara Canis ◽  
Meriones Unguiculatus ◽  
Mongolian Gerbils ◽  
Igg Antibodies ◽  
Chain Reaction ◽  
Specific Igg ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Specific Igg Antibodies

AbstractToxocariasis is a common human zoonosis, which induces a clinically unapparent course of infection. Diagnosis is difficult and relies upon serological testing (searching of specific IgG antibodies by ELISA), laboratory abnormalities and clinical manifestations. The polymerase chain reaction (PCR) technique was adapted for the detection of Toxocara canis larvae in a host tissue. Mongolian gerbils (Meriones unguiculatus) were used as an animal model for human toxocariasis. 8 animals were inoculated with 1000 T. canis eggs, four uninfected were used as control. At 3, 5, 7, and 14 days post-infection, 2 infected and 1 control gerbil were killed and their livers were used for molecular analysis. Specific primer in the PCR reaction allowed identification of T. canis larvae, with the parasite gDNA found in the liver of all infected gerbils. The results indicate that the PCR method has a potential as a supporting technique for the diagnosis of human toxocariasis.

scholarly journals Contribution to canine babesiosis in the Czech Republic

2012 ◽  
Vol 81 (2) ◽  
pp. 91-95 ◽  
Author(s):  
Jarmila Konvalinová ◽  
Ivo Rudolf ◽  
Silvie Šikutová ◽  
Zdeněk Hubálek ◽  
Vlasta Svobodová ◽  
...  
Keyword(s):  
Czech Republic ◽  
Dermacentor Reticulatus ◽  
Igg Antibodies ◽  
Canine Babesiosis ◽  
The Czech Republic ◽  
Negative Results ◽  
Working Dogs ◽  
Specific Igg ◽  
Polymerase Chain ◽  
Specific Igg Antibodies

From March to November 2010, a total of 68 samples of blood from 41 hunting and working dogs that never left the Czech Republic were examined. Some dogs were sampled repeatedly. Blood samples were examined by polymerase chain reaction for the presence of DNA of piroplasms with negative results. Specific IgG antibodies against Babesia canis were detected by indirect immunofluorescence test, and five dogs (12.21%) were seropositive. Titres ranged from 50 to 200. One dog was positive in two samplings within 3 months. The highest number of positive samples was taken in June. The results of this study suggest a likely contact of the examined dogs with the parasite; although in 2005, a total of 340 adult unfed Dermacentor reticulatus ticks in 34 pools screened by PCR for babesiae were negative.

Molecular, serological and epidemiological observations after a suspected outbreak of plague in Nyimba, eastern Zambia

2016 ◽  
Vol 47 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Stanley S Nyirenda ◽  
Bernard M Hang’ombe ◽  
Bukheti S Kilonzo ◽  
Mathews N Kabeta ◽  
Mundia Cornellius ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Yersinia Pestis ◽  
Plasminogen Activator ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Recent Past ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Pcr Techniques ◽  
Suspected Outbreak

Plague is a re-emerging zoonotic disease caused by the bacterium Yersinia pestis. The disease has caused periodic global devastation since the first outbreak in the 6th century. Two months after a suspected plague outbreak in Nyimba district, samples were collected from 94 livestock (goats and pigs), 25 rodents, 6 shrews and 33 fleas. Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) techniques were used to investigate the presence of Y. pestis, which showed that 16.0% (4/25) of rodents, 16.7% (1/6) of shrews ( Crocidura spp) and 6.0% (5/83) of goats were positive for IgG antibodies against Fraction 1 antigen of Y. pestis. Plasminogen activator (Pla) gene (DNA) of Y. pestis was detected in five pools containing 36.4% (12/33) fleas collected from pigs (n = 4), goats (n = 5) and rodents (n = 3). The detection of Pla gene in fleas and IgG antibodies against Fraction1 antigen in rodents, shrews and goats suggest that Y. pestis had been present in the study area in the recent past.

scholarly journals Previous Syphilis Alters the Course of Subsequent Episodes of Syphilis

2019 ◽  
Vol 71 (5) ◽  
pp. 1243-1247 ◽  
Author(s):  
Christina M Marra ◽  
Clare L Maxwell ◽  
Sharon K Sahi ◽  
Lauren C Tantalo ◽  
Shelia B Dunaway ◽  
...  
Keyword(s):  
Public Health ◽  
Polymerase Chain Reaction ◽  
Cerebrospinal Fluid ◽  
Logistic Regression ◽  
Treponema Pallidum ◽  
Chain Reaction ◽  
Serological Tests ◽  
Health Records ◽  
Polymerase Chain ◽  
Diagnosis Date

Abstract Background Individuals with previous syphilis may be more likely to be asymptomatic when they are reinfected with Treponema pallidum. Methods Individuals enrolled in a study of cerebrospinal fluid (CSF) abnormalities in syphilis were allowed to enroll in the study again with subsequent syphilis. For each participant, the index episode was defined as the most recent syphilis episode for which the study entry visit was performed within 30 days of the syphilis diagnosis date. Venipuncture and lumbar puncture were performed. The total number of syphilis episodes was determined by review of medical and public health records. Treponema pallidum DNA in blood and rRNA in CSF were detected using polymerase chain reaction (PCR) and reverse transcriptase PCR. Odds ratios (ORs) with 95% confidence intervals (CIs) were determined using logistic regression. Results 701 individuals had 1 (n = 478), 2 (n = 155), or ≥3 (n = 68) episodes of syphilis. The proportion of individuals whose index episode was asymptomatic significantly increased with increased number of syphilis episodes (P < .001). This difference was not explained by frequency of serological tests. Adjusted ORs (aORs) of detection of T. pallidum DNA in blood or rRNA in CSF at the index episode were significantly lower in those with previous syphilis (0.13; 95% CI, .08–.23, and 0.06, 95% CI, .02–.17). The aOR of neurosyphilis at the index episode was also significantly lower in individuals with previous syphilis (0.43; 95% CI, .27–.68). Conclusions Previous syphilis attenuates clinical and laboratory manifestations of infection with T. pallidum.

Management of patients with systemic autoimmune diseases with the active phase of chronic herpes simplex infection

2020 ◽  
Vol 26 (2-3) ◽  
pp. 21-27
Author(s):  
I.G. Gaiduchok ◽  
◽  
Kh.O. Lishchuk-Yakymovych ◽  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex ◽  
Autoimmune Diseases ◽  
Virus Replication ◽  
Active Phase ◽  
Alpha Interferon ◽  
Igg Antibodies ◽  
Chain Reaction ◽  
Systemic Autoimmune Diseases ◽  
Polymerase Chain

Aim. To study the effectiveness of acyclovir in patients with systemic autoimmune diseases caused by active chronic herpes simplex 1/2 infection. Material and Methods. Among 380 patients with systemic autoimmune diseases (systemic lupus erythematosus, systemic vasculitis, rheumatoid arthritis, psoriasis) in 45 (11.8%) patients was diagnosed active phase of chronic HSV1/2 infection with help of viral DNA identification by polymerase chain reaction. These patients received acyclovir in three courses with a one-month interval between them. The effectiveness of treatment was monitored before and after treatment with help of virus DNA results in three bioenvironments (blood, saliva and swab from the lesion), of general and specific IgM, IgG antibodies concentration; levels of IgE, cryoglobulins, circulating immune complexes, alpha-interferon, C3-component of complement, the number of lymphocytes populations/subpopulations and of activated cells. Results and Discussion. After the treatment, it was fixed as significant decrease of specific IgM, IgG antibodies concentration as of the level of total IgE, cryoglobulins and cryofibrinogen. In addition it was observed as significant decreased level of alpha-interferon in the serum and saliva, as of the natural killers and number of lymphocytes, expressing the low-affinity receptor IL2 (CD25+) and lymphocytes with suppressive activity (CD4+25+). After the treatment it was observed by polymerase chain reaction a decrease of virus replication in 66.7% of cases. Conclusions. The results of the study indicate, that the use of acyclovir for the treatment of active phase of chronic HSV 1/2-infection might contribute as to the decrease in the virus replication, reducing the viral load, as to the suppression of aggressive autoimmune reactions, reducing the risk of allergopathology development. Key words: systemic autoimmune diseases, herpes simplex viruses, antiviral therapy

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