scholarly journals Contribution to canine babesiosis in the Czech Republic

2012 ◽  
Vol 81 (2) ◽  
pp. 91-95 ◽  
Author(s):  
Jarmila Konvalinová ◽  
Ivo Rudolf ◽  
Silvie Šikutová ◽  
Zdeněk Hubálek ◽  
Vlasta Svobodová ◽  
...  
Keyword(s):  
Czech Republic ◽  
Dermacentor Reticulatus ◽  
Igg Antibodies ◽  
Canine Babesiosis ◽  
The Czech Republic ◽  
Negative Results ◽  
Working Dogs ◽  
Specific Igg ◽  
Polymerase Chain ◽  
Specific Igg Antibodies

From March to November 2010, a total of 68 samples of blood from 41 hunting and working dogs that never left the Czech Republic were examined. Some dogs were sampled repeatedly. Blood samples were examined by polymerase chain reaction for the presence of DNA of piroplasms with negative results. Specific IgG antibodies against Babesia canis were detected by indirect immunofluorescence test, and five dogs (12.21%) were seropositive. Titres ranged from 50 to 200. One dog was positive in two samplings within 3 months. The highest number of positive samples was taken in June. The results of this study suggest a likely contact of the examined dogs with the parasite; although in 2005, a total of 340 adult unfed Dermacentor reticulatus ticks in 34 pools screened by PCR for babesiae were negative.

scholarly journals Examination of convalescents after West Nile fever for the specific IgM, IgG and neutralizing antibodies

2016 ◽  
Vol 21 (2) ◽  
pp. 82-86
Author(s):  
Alla R. Azaryan ◽  
A. A Kozlova ◽  
A. P Grishanova ◽  
E. I Ivashchenko ◽  
G. L Shendo ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Igg Antibodies ◽  
West Nile Fever ◽  
Serum Samples ◽  
West Nile ◽  
Negative Results ◽  
Igm Elisa ◽  
Control Research ◽  
Specific Igg ◽  
Specific Igg Antibodies

The examination was executed in the Astrakhan region over 2013-2014 with participation of three laboratories. In 26 convalescents after West Nile Fever (WNF) with the serological confirmed diagnosis of WNF blood sera were examined. Neurological form of WNF was observed in 8 cases, and febrile form - in 8 convalescents. Sera have been collected in 243-358 days, on average, in 308 days after the beginning of a disease. For their examination there were used IFA-IgM ELISA (MAC-ELISA), IgG ELISA methods as well as neutralization test in Vero E6 cell culture. The results of the examination in 24 of 26 patients (92, 3%) for IgM antibodies to the virus WNF were negative. In two convalescents according to the laboratories in Astrakhan and Moscow in sera there were observed low titers of IgM (1:400) with minor indices of sera optical density (0.3 to 0.4) and negative results in the Volgograd Plague Control Research Institute. Serum samples of other two convalescents were weakly positive or questionable in testing in Volgograd, but were negative when examined in the Institute of Virology. Specific IgG antibodies were detected in 23 of 26 convalescents (88.5%), neutralizing in 22 of 24 (91.7 %). These data confirm the adequacy of the criteria and tactics for WNF serodiagnosis adopted in Russia based on the application of the MAC -ELISA (IFA - IgM).

scholarly journals SEROPREVALENCE OF ANTI-MEASLES IGG IN THE POPULATION OF KHARKIV REGION IN 2018-2019

2019 ◽  
pp. 22-25
Author(s):  
Igor Yudin ◽  
Irina Kuchma ◽  
Andriy Voliansky
Keyword(s):  
Herd Immunity ◽  
Age Groups ◽  
Igg Antibodies ◽  
Prevention Strategies ◽  
Elisa Method ◽  
Negative Results ◽  
Specific Igg ◽  
Susceptible Populations ◽  
Vaccination Campaigns ◽  
Specific Igg Antibodies

Aim. The aim of this study was to evaluate herd immunity against measles in different age groups of the Kharkiv region of Ukraine’s population. Materials and methods. Seroprevalence of specific IgG antibodies was studied among different age groups of the Kharkiv region population from February 2018 to July 2019 by ELISA method. Results. A number of 6564 sera samples were processed. The overall seropositivity level was 91.3%; negative results were 5.3% and 3.3% equivocal. The highest seropositivity for IgG (> 98%) was in people aged 50 years and older. The lowest seropositivity rates were observed in the cohorts of 20-29 years (87.2%), 30-39 years (86.9%), and 40-49 years (91.1%). Conclusions. Measles-susceptible populations were identified for which new prevention strategies should be applied in the form of personalized vaccination campaigns to increase herd immunity.

scholarly journals Characteristics of Ebola Virus Disease Survivor Blood and Semen in Liberia: Serology and Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2020 ◽  
Author(s):  
Aaron Kofman ◽  
Susanne Linderman ◽  
Kaihong Su ◽  
Lawrence J Purpura ◽  
Elizabeth Ervin ◽  
...  
Keyword(s):  
Ebola Virus ◽  
Virus Disease ◽  
Igg Antibodies ◽  
Chain Reaction ◽  
Male Survivors ◽  
Qrt Pcr ◽  
Specific Igg ◽  
Polymerase Chain ◽  
Study Participants ◽  
Specific Igg Antibodies

Abstract Introduction Ebola virus (EBOV), species Zaire ebolavirus, may persist in the semen of male survivors of Ebola virus disease (EVD). We conducted a study of male survivors of the 2014–2016 EVD outbreak in Liberia and evaluated their immune responses to EBOV. We report here findings from the serologic testing of blood for EBOV-specific antibodies, molecular testing for EBOV in blood and semen, and serologic testing of peripheral blood mononuclear cells (PBMCs) in a subset of study participants. Methods We tested for EBOV RNA in blood by quantitative reverse transcription polymerase chain reaction (qRT-PCR), and for anti-EBOV-specific immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies by enzyme-linked immunosorbent assay (ELISA) for 126 study participants. We performed PBMC analysis on a subgroup of 26 IgG-negative participants. Results All 126 participants tested negative for EBOV RNA in blood by qRT-PCR. The blood of 26 participants tested negative for EBOV-specific IgG antibodies by ELISA. PBMCs were collected from 23/26 EBOV IgG-negative participants. Of these, 1/23 participants had PBMCs that produced anti-EBOV-specific IgG antibodies upon stimulation with EBOV-specific glycoprotein (GP) and nucleoprotein (NP) antigens. Conclusions The blood of EVD survivors, collected when they did not have symptoms meeting the case definition for acute or relapsed EVD, is unlikely to pose a risk for EBOV transmission. We identified 1 IgM/IgG negative participant who had PBMCs that produced anti-EBOV-specific antibodies upon stimulation. Immunogenicity following acute EBOV infection may exist along a spectrum, and absence of antibody response should not be exclusionary in determining an individual’s status as a survivor of EVD.

scholarly journals Detection of Toxocara canis larvae by PCR in the liver of experimentally infected Mongolian gerbils (Meriones unguiculatus)

2008 ◽  
Vol 45 (3) ◽  
pp. 147-149 ◽  
Author(s):  
A. Borecka ◽  
J. Gawor ◽  
M. Niedworok ◽  
B. Sordyl
Keyword(s):  
Clinical Manifestations ◽  
Toxocara Canis ◽  
Meriones Unguiculatus ◽  
Mongolian Gerbils ◽  
Igg Antibodies ◽  
Chain Reaction ◽  
Specific Igg ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Specific Igg Antibodies

AbstractToxocariasis is a common human zoonosis, which induces a clinically unapparent course of infection. Diagnosis is difficult and relies upon serological testing (searching of specific IgG antibodies by ELISA), laboratory abnormalities and clinical manifestations. The polymerase chain reaction (PCR) technique was adapted for the detection of Toxocara canis larvae in a host tissue. Mongolian gerbils (Meriones unguiculatus) were used as an animal model for human toxocariasis. 8 animals were inoculated with 1000 T. canis eggs, four uninfected were used as control. At 3, 5, 7, and 14 days post-infection, 2 infected and 1 control gerbil were killed and their livers were used for molecular analysis. Specific primer in the PCR reaction allowed identification of T. canis larvae, with the parasite gDNA found in the liver of all infected gerbils. The results indicate that the PCR method has a potential as a supporting technique for the diagnosis of human toxocariasis.

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