scholarly journals Endoparasites in European hares (Lepus europaeus) under gamekeeping conditions in the Czech Republic

2012 ◽  
Vol 49 (3) ◽  
pp. 159-163 ◽  
Author(s):  
D. Lukešová ◽  
I. Langrová ◽  
J. Vadlejch ◽  
I. Jankovská ◽  
J. Hlava ◽  
...  
Keyword(s):  
Czech Republic ◽  
Lepus Europaeus ◽  
The Czech Republic ◽  
Faecal Samples ◽  
Eimeria Spp ◽  
Prevalent Species ◽  
Different Parts

AbstractDuring December 2007 and November–April 2008/2009, 128 gastrointestinal tracts and 1680 faecal samples of hares from different parts of the Czech Republic were examined. Evaluation of the faeces samples indicated that the samples could have been infected with any one of the following parasites: Eimeria spp., Trichostrongylus retortaeformis, Trichuris leporis, Graphidium strigosum, Strongyloides sp., Protostrongylus spp. and Anoplocephalidae tape-worms. Trichostrongylus retortaeformis was the most common nematode (75.8 %). 39.8 % of the animals were infected with Trichuris leporis. Only four hares were in-fected with Graphidium strigosum. Eimeria spp. was the most commonly occurring parasite (90.5 %). The follow-ing species were found: Eimeria coquelinae, E. cabareti, E. leporis, E. babatica, E. europea, E. pierrecouderti, E. macrosculpta, E. orbiculata and E. stefanski. Eimeria coquelinae was the most prevalent species (68.4 %). Eimeria cabareti and E. leporis were the next most frequently found species (64.5 % and 57.8 %, respectively).

scholarly journals Eimeriosis Seasonal Dynamics Patterns at an Organic Sheep Farm in the Czech Republic

2017 ◽  
Vol 48 (2) ◽  
pp. 70-75
Author(s):  
I.A. Kyriánová ◽  
J. Vadlejch ◽  
I. Langrová
Keyword(s):  
Czech Republic ◽  
Species Composition ◽  
Eimeria Species ◽  
The Czech Republic ◽  
Sheep Farm ◽  
Faecal Samples ◽  
Flotation Technique ◽  
Prevalent Species ◽  
Genus Eimeria ◽  
Eimeria Infection

Abstract This epidemiological study monitored the occurrence of the coccidia genus Eimeria and their species composition over a oneyear period at an organic sheep farm in the Czech Republic. Individual faecal samples were collected from thirty lambs and thirty ewes once a month throughout the whole survey. As a result, 348 and 333 samples from ewes and lambs respectively were evaluated using the faecal flotation technique. The overall prevalence of eimeriosis was 75.7% and 54.0% for lambs and ewes respectively. Four Eimeria species (E. ovinoidalis, E. crandallis/weybridgensis, E. parva, and E. intricata) were identified in both, lambs and ewes, during this survey. The most prevalent species was E. ovinoidalis, with an overall prevalence of 84% in ewes and 85% in lambs, followed by E. parva and E. crandallis/weybridgensis. The oocysts faecal output was seasonal. The highest oocysts per gram levels were detected in February (139 000) and May (250 000) in ewes and in February (1 949 900), March (326 000), and May (187 700) in lambs. The intensity of Eimeria infection differed significantly (P < 0.0001) between ewes and lambs during the monitored period.

scholarly journals  Parasite load of European brown hares in Austria and the Czech Republic

2012 ◽  
Vol 57 (No. 10) ◽  
pp. 551-558 ◽  
Author(s):  
K. Chroust ◽  
M. Vodnansky ◽  
J. Pikula
Keyword(s):  
Body Weight ◽  
Czech Republic ◽  
Gastrointestinal Nematode ◽  
Parasite Load ◽  
The Body ◽  
Lepus Europaeus ◽  
Parasitic Infections ◽  
Regression Equations ◽  
The Czech Republic ◽  
Eimeria Spp

&nbsp; The parasite load of brown hares (Lepus europaeus) is of great interest to hunting ground managers and veterinarians. We compared the prevalence and intensity of parasitic infections in 362 hares from Austria and the Czech Republic with respect to age and body weight. Samples of the entire gastrointestinal tract, liver and lungs were collected during autumn hunting events in 2007. The parasite spectrum of hares included Protostrongylus pulmonalis, Graphidium strigosum, Trichostrongylus retortaeformis, Trichuris leporis, Eimeria spp. and tapeworms. The most prevalent gastrointestinal nematode was Trichostrongylus retortaeformis, while only individual specimens of tapeworms such as Andrya rhopalocephala, Mosgovoyia pectinata, Cittotaenia denticulata and Ctenotaenia ctenoides were found in subadult hares. A single hare was infected with Cysticercus pisiformis in Austria. Lungworms Protostrongylus pulmonalis and findings of pneumonia were significantly less prevalent in subadult than adult hares (P &lt; 0.01) from both countries and were much less prevalent overall in the Czech Republic (P &lt; 0.01). Graphidium strigosum, Trichostrongylus retortaeformis, Eimeria spp. and enteritis were more prevalent in subadult hares. The nematode Trichuris leporis, on the other hand, prevailed in adults. The body weight of adult hares was negatively correlated with the intensity of infection by Protostrongylus pulmonalis (r = &ndash;0.67) and Trichostrongylus retortaeformis (r = &ndash;0.73) and the parasite loads served as significant weight predictors in multiple regression equations. This study revealed that parasitic infections of the lungs and intestines influences the health and decreases the body weight of hares in Austrian and Czech hunting grounds. &nbsp;

scholarly journals Spiders of the Vine Plants in Southern Moravia

2015 ◽  
Vol 63 (5) ◽  
pp. 1471-1476
Author(s):  
Lucie Havlová ◽  
Vladimír Hula ◽  
Jana Niedobová
Keyword(s):  
Czech Republic ◽  
Soil Management ◽  
Common Species ◽  
Red List ◽  
The Other ◽  
Important Species ◽  
The Czech Republic ◽  
Spider Species ◽  
Southern Moravia ◽  
Different Parts

Araneofauna of vineyards is relatively known in Central Europe but we have a lack of knowledge about araneofauna which occur directly on the vine plants. Our investigation was focused on spiders which live on vine plants, especially on the vine plants trunks. We investigated spiders in six vineyards in southern Moravia (Šatov, Mikulov, Popice, Morkůvky, Nosislav and Blučina). Vineyards were under different soil management, traps were placed on different parts of particular locality (terraced and plain) and all localities were under integrated pest management. We employed two types of cardboard traps for spider collecting during whole vegetation season. Altogether, we collected 21 spider species which belong to seven families. The most important species was Marpissa nivoyi (Lucas, 1836), which is mentioned in the Red List as vulnerable (VU) and Sibianor tantulus (Simon, 1868) which had unknown distribution in the Czech Republic. The other very interesting result is that the most common species is myrmecomorph Synageles venator (Lucas, 1836), which is scarcely recorded in such huge numbers as we documented in our study.

scholarly journals Resistance behaviour of Septoria tritici to some fungicides in the territory of the Czech Republic

2012 ◽  
Vol 58 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Ludvik Tvaružek ◽  
P. Horáková ◽  
L. Ji
Keyword(s):  
Czech Republic ◽  
Septoria Tritici ◽  
Resistance Development ◽  
The Czech Republic ◽  
Colony Diameter ◽  
Dmi Fungicides ◽  
The Mean ◽  
Different Parts ◽  
Pathogen Strains

Reduction of colony diameter using two DMI-fungicides and one strobilurine fungicide was evaluated in the <i>Septoria tritici</i> population. In an <i>in vitro</i> assay, 57 strains of <i>S. tritici</i> originating from different parts of the Czech Republic were grown on agar plates containing different concentrations of fungicides. The mean EC<sub>50</sub> values of strains were analysed for flusilazole (Capitain), metconazole (Caramba) and one strobilurine fungicide: azoxystrobin (Amistar). ED 50 values for metconazole were low. There were highly significant correlation in reaction of pathogen strains to metconazole and flusilazole. Some isolates showed significant higher level of resistance to azoxystrobin. The data will be used in a continuing survey of resistance development in the <i>S. tritici</i> population originating from the territory of the Czech Republic.

scholarly journals Parasites of Myocastor coypus – a Comparison in Farm Animals and Their Feral Counterparts

2018 ◽  
Vol 49 (1) ◽  
pp. 21-25
Author(s):  
S. Nechybová ◽  
I. Langrová ◽  
E. Tůmová
Keyword(s):  
Czech Republic ◽  
Nematode Species ◽  
Farm Animals ◽  
Myocastor Coypus ◽  
Free Living ◽  
The Czech Republic ◽  
Faecal Samples ◽  
Nematode Eggs ◽  
Mcmaster Method ◽  
Feral Animals

Abstract The parasitic status in the coypus (nutrias) Myocastor coypus, both farm-bred or free-living on river banks in the Czech Republic, was determined. Faecal samples were collected from 200 coypus originating from 11 farms (farm-bred animals) and from 20 individuals living at 14 natural localities (feral animals). Faeces were examined for nematode eggs and coccidian oocysts using the McMaster method. The evaluation of faeces from farm-bred coypus indicated infection with the following parasites: Trichuris sp., Strongyloides sp., Trichostrongylus sp., Eimeria seidelli, Eimeria nutriae, Eimeria coypi, and Eimeria myopotami. Free-living feral rodents harboured Eimeria nutriae, E. coypi, Strongyloides sp. and in one case also Trichuris sp. An additional visceral examination of 20 coypus originating from five farms revealed two nematode species in the gastrointestinal tracts, namely Trichuris myocastoris and Strongyloides myopotami. The study indicated that feral coypus are far less parasitized than their captive counterparts.

scholarly journals Physiologic Specialization of Wheat Leaf Rust (Puccinia triticina Eriks.) in theCzech Republic in 2001–2004

2011 ◽  
Vol 42 (No. 4) ◽  
pp. 126-131 ◽  
Author(s):  
A. Hanzalová ◽  
P. Bartoš
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Puccinia Triticina ◽  
Field Trials ◽  
Wheat Leaf Rust ◽  
The Czech Republic ◽  
Wheat Leaf ◽  
Winter Wheat Cultivars ◽  
Physiologic Races ◽  
Different Parts

In 2001&ndash;2004 virulence of the wheat leaf rust population in the Czech Republic was studied on Thatcher near-isogenic lines with Lr1, Lr2a, Lr2b, Lr2c, Lr3a, Lr9, Lr10, Lr11, Lr15, Lr17, Lr19, Lr21, Lr23, Lr24, Lr26 and Lr28. Samples of leaf rust (180 in total) were obtained from different parts of the Czech Republic. Resistance genes Lr9 and Lr19 were effective to all tested isolates like in the previous years. Unlike the previous years virulence on Lr24 and Lr28 was observed. Relatively effective were also Lr1, Lr2a, Lr24, Lr28 and Lr2b. Other Lr genes were defeated by the majority of the tested samples. Our results transformed to the numbers of physiologic races indicate that race 61SaBa prevailed like in the previous years, followed by races 61, 2, 12SaBa, 2SaBa, 14, 77SaBa, 12, 57, 6, 53, 53SaBa, 77, and 14SaBa. Twenty-one winter wheat cultivars registered in 2001&ndash;2004 were tested with 8 leaf rust isolates. Out of them 15 showed resistance at least to one rust isolate. High resistance recorded in field trials for cvs Batis and Ilias, which were susceptible to all tested rust isolates at the seedling stage, demonstrates the importance of field (partial) resistance. &nbsp;

scholarly journals Molecular characterization of a porcine sapovirus strain isolated from a piglet with diarrhoea: a case report

2011 ◽  
Vol 56 (No. 8) ◽  
pp. 409-415 ◽  
Author(s):  
L. Dufkova ◽  
P. Kulich ◽  
J. Prodelalova
Keyword(s):  
Czech Republic ◽  
Rna Polymerase ◽  
The Czech Republic ◽  
Porcine Sapovirus ◽  
Viral Particles ◽  
Faecal Samples ◽  
The Family ◽  
Capsid Gene Sequence ◽  
Negative Staining Electron Microscopy

Porcine sapoviruses, members of the family Caliciviridae, have been considered as an aetiological agent of gastroenteritis in pigs. In this study, we analysed 251 faecal samples obtained from 3 to 90 day-old diarrhoeic pigs in the Czech Republic between January 2005 and June 2010 and tested them by negative staining electron microscopy for the presence of sapoviruses. Only one sample showed the presence of viral particles with characteristic sapovirus morphology. The presence of sapovirus (SaV) was confirmed by an RT-PCR assay with primers specific for the sapoviral RNA polymerase and capsid genes. Phylogenetic analysis based on a partial sequence of the RNA polymerase gene placed the new Czech isolate into the GVII genogroup of porcine sapoviruses; however, analysis of a portion of the capsid gene sequence classified the isolate as GIII of the genus Sapovirus. These contradictory findings indicate that recombinant porcine sapovirus was identified. According to our knowledge this is the first description of porcine sapovirus in domestic pigs in the Czech Republic

scholarly journals The wildlife hosts of Mycobacterium avium subsp. paratuberculosis in the Czech Republic during the years 2002–2007

2008 ◽  
Vol 53 (No. 8) ◽  
pp. 420-426 ◽  
Author(s):  
M. Kopecna ◽  
I. Trcka ◽  
J. Lamka ◽  
M. Moravkova ◽  
P. Koubek ◽  
...  
Keyword(s):  
Czech Republic ◽  
Wild Boar ◽  
Mycobacterium Avium ◽  
Columba Livia ◽  
Mycobacterium Avium Subsp ◽  
Lepus Europaeus ◽  
The Czech Republic ◽  
Terrestrial Mammals ◽  
Rflp Type ◽  
Mycobacterium Avium Subsp Paratuberculosis

The objective of this study was to determine the wildlife hosts of Mycobacterium avium subsp. paratuberculosis (MAP) in the Czech Republic. A total of 8 796 wildlife animals were examined by culture of faecal or tissue samples during the years 2002–2007. MAP was isolated from 12 (0.5%) out of 2 296 red deer (Cervus elaphus), two (0.2%) out of 835 roe deer (Capreolus capreolus), 78 (5.7%) out of 1 381 fallow deer (Dama dama), 28 (3.2%)out of 866 mouflons (Ovis musimon), four (2.5%) out of 162 chamois (Rupicapra rupicapra) and from one (0.1%) out of 805 wild boar (Sus scrofa). MAP was not cultured from 82 badgers (Meles meles), 55 martens (Martes foina), one pine marten (Martes martes), 25 brown hares (Lepus europaeus), five rabbits (Oryctolagus cuniculus), nine European polecats (Mustela putorius), two steppe polecats (Mustela eversmannii), two American minks (Mustela vison), four raccoon dogs (Nyctereutes procyonoides) and four Eurasian otters (Lutra lutra). MAP was isolated from three (2.0%) out of 149 small terrestrial mammals: one (5.9%) out of 17 brown rats (Rattus norvegicus), one (1.7%) out of 59 common voles (Microtus arvalis) and one (2.6%) out of 39 lesser white-toothed shrews (Crocidura suaveolens). Culture examinations of 34 house mice (Mus musculus) and 2 113 pigeons (Columba livia f. domestica) were negative. All 123 in vitro growing MAP isolates from wild ruminants were of IS900 RFLP type B-C1. One mouflon infected with a MAP strain which did not grow on the tested media was after IS1311-PRA-PCR assessed as being infected with a “sheep” strain. The RFLP type of the MAP isolate from the wild boar was of the RFLP type A-C10. Although the detection of MAP in wildlife in the Czech Republic was not very high, their role as a potential risk factor for cattle should be considered.

Seroprevalence of Neospora caninum in cats from the Czech Republic

2014 ◽  
Vol 59 (2) ◽  
Author(s):  
Kamil Sedlák ◽  
Eva Bartova ◽  
Tereza Machacova
Keyword(s):  
Czech Republic ◽  
Competitive Inhibition ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Domestic Cats ◽  
The Czech Republic ◽  
Indirect Fluorescence Antibody ◽  
Different Parts ◽  
Sera Samples

AbstractSera of 414 cats coming from different parts of the Czech Republic were tested for N. caninum antibodies. Sera samples were collected during years 2002–2011. N. caninum antibodies were detected by a commercial competitive-inhibition enzyme-linked immunosorbent assay (cELISA) with cut off ≥30% inhibition. Samples positive in cELISA were confirmed by an indirect fluorescence antibody test (IFAT); titre ≥50 was considered positive. In total, 137 (33%) cats reacted positively in cELISA; N. caninum antibodies in IFAT were detected in 16 (3.86%) cats with titres 50 and 100. In 6 cats, positive for N. caninum antibodies, T. gondii antibodies were also detected by IFAT. It is the first report of N. caninum antibodies in domestic cats from the Czech Republic and third report in Europe.

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