SEM observation of pollen grains and fruits of the Pieniny Mountains Polish endemic species Taraxacum pieninicum (Asteraceae)

Biologia ◽  
2010 ◽  
Vol 65 (2) ◽  
Author(s):  
Leszek Bednorz ◽  
Irmina Maciejewska-Rutkowska
Keyword(s):  
Endemic Species ◽  
Pollen Morphology ◽  
External Surface ◽  
Pollen Grains ◽  
Medium Size ◽  
Tubular Cells ◽  
Sem Observation ◽  
Taraxacum Pieninicum

AbstractTaraxacum pieninicum Pawł. (Asteraceae) is an endemic species, growing only in Pieniny Mts. The aim of the presented work was to investigate its pollen morphology as well as its ultrastructural traits of achene pericarp. Pollen grains of this species were of medium size, echino-lophate and 3-zonocolporate. The external surface of the achene body was of scale sculpture, covered with the spikes. Pappus hairs were not branched, built of a few tubular cells, with acute, protruding ends.

Pollen characterization of ornamental species of Mammillaria Haw. (Cactaceae/Cactoideae)

2019 ◽  
Vol 6 (1) ◽  
pp. 13 ◽  
Author(s):  
Denise M. D. S. Mouga ◽  
Gabriel R. Schroeder ◽  
Nilton P. Vieira Junior ◽  
Enderlei Dec
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Radial Symmetry ◽  
Morphological Data ◽  
Medium Size ◽  
Ornamental Species

The pollen morphology of thirteen species of Cactaceae was studied: M. backebergiana F.G. Buchenau, M. decipiens Scheidw, M. elongata DC, M. gracilis Pfeiff., M. hahniana Werderm., M. marksiana Krainz, M. matudae Bravo, M. nejapensis R.T. Craig & E.Y. Dawson, M. nivosa Link ex Pfeiff., M. plumosa F.A.C. Weber, M. prolifera (Mill.) Haw, M. spinosissima var. “A Peak” Lem. and M. voburnensis Scheer. All analysed pollen grains are monads, with radial symmetry, medium size (M. gracilis, M. marksiana, M. prolifera, large), tricolpates (dimorphs in M. plumosa [3-6 colpus] and M. prolifera [3-6 colpus]), with circular-subcircular amb (quadrangular in M. prolifera and M. plumosa with six colpus). The pollen grains presented differences in relation to the shape and exine thickness. The exine was microechinate and microperforated. The pollen morphological data are unpublished and will aid in studies that use pollen samples. These pollen grains indicate ornamental cacti.

scholarly journals Pollen morphology of the Balkan-Carpathian endemic Campanula lingulata Waldst. & Kit. (Campanulaceae)

2016 ◽  
pp. 75-82
Author(s):  
Marina Macukanovic-Jocic ◽  
Snezana Jaric
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Endemic Species ◽  
Pollen Morphology ◽  
Average Length ◽  
Pollen Grains ◽  
Polar Axis ◽  
First Time ◽  
Scanning Electron

Palynomorphological characteristics of Campanula lingulata, the Balkan-Carpathian endemic species growing in Serbia, have been investigated using light microscopy and scanning electron microscopy for the first time, in order to provide some information helpful for a better understanding of the taxonomic position of this species within the genus, as well as to contribute to the pollen atlas of Serbian apiflora. The pollen grains are radially symmetrical, isopolar, 3-zonoporate and medium-sized monads oblate-sphaeroidal in shape. Mean of the polar axis (P) is 27.6?1.9 ?m, while the average length of the equatorial axis (E) is 28.8?1.6 ?m. The apertures are operculate. The sculpturing pattern of the exine is microre?ticulate-microechinatae. The exine surface is covered with evenly distributed supratectal spinules of variable length and sparse granules. The longest supratectal spinules are 0.64?0.05 ?m in length and the smallest sculptural elements are less than 0.2 ?m high. The microechinae density per sample area of 5 ?m x 5 ?m averages 17.4?2.4.

scholarly journals Pollen morphology of Myrtaceae visited by social bees

2019 ◽  
Vol 6 (2) ◽  
pp. 98-105
Author(s):  
Andreia Santos do Nascimento ◽  
Carlos Alfredo Lopes de Carvalho
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Morphological Characters ◽  
Medium Size ◽  
Morphological Description ◽  
Pollen Spectrum ◽  
Social Bees ◽  
Equatorial Diameter ◽  
Polar Diameter

This study aimed to characterize species of Myrtaceae belonging to genera already identified in the pollen spectrum of beehive products of social bees, describing pollen grains morphology and correlating with taxonomic differences the species reported in the literature. For each plant species, measurements were made with 25 pollen grains in equatorial view and the polar diameter (PD) and equatorial diameter (ED) were measured. The exine thickness was obtained by measuring 10 pollen grains in polar view at the medium height of mesocolpium. The description of pollen morphology showed that pollen grains of the species are triangular amb, 3-colporates, psilate surface, small to medium size and oblate shape with the P/E ratio ranging from 0.52 to 0.72 µm. The morphological description of Myrtaceae species showed similarity between several characteristics common to pollen grains of this species. The description also allowed separation of nine species studied by size and specific morphological characters with the identification key, which can also be used to study the pollen spectrum of hive products.

scholarly journals Pollen morphology of the Polish species of the genus Sorbus L.

2011 ◽  
Vol 74 (4) ◽  
pp. 315-322 ◽  
Author(s):  
Leszek Bednorz ◽  
Irmina Maciejewska ◽  
Dorota Wrońska-Pilarek ◽  
Toshiyuki Fujiki
Keyword(s):  
Native Species ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Medium Size ◽  
Sorbus Aucuparia ◽  
Prolate Spheroidal ◽  
Medium Thickness ◽  
Diagnostic Features ◽  
Systematic Classification ◽  
Close Relationship

This paper presents the results of the study on pollen morphology of five Polish species of the genus <em>Sorbus</em> L. (<em>S. aria</em> (L.) Crantz, <em>S. aucuparia</em> L. Emend. Hedl., <em>S. chamaemespilus</em> (L.) Crantz, <em>S. intermedia</em> (Ehrh.) Pers. and <em>S. torminalis</em> (L.) Crantz). Observations were carried out with light and scanning microscopes. Investigated pollen grains were small or medium size, most often subprolate or prolate spheroidal in outline. Exine of medium thickness, consisted of two layers. Mainly 3-zonocolporate or sometimes 4-zonocolporate (<em>S. chamaemespilus</em> and <em>S. torminalis</em>) grains were noted. Usually striatae type of sculpture was observed, except the grains of <em>S. intermedia</em>, which were characterized by intermediate type of sculpture, with the features both striate and regulate ornamentation. Pollen morphology of the investigated <em>Sorbus</em> species corresponds with their systematic classification. It is relatively easy to separate pollen grains of the following subgenera: <em>Sorbus</em>, <em>Chamaemespilus</em> and <em>Torminaria</em>. Only subgenus <em>Aria</em> includes grains of different morphology and therefore it is impossible to determine the diagnostic features of this subgenus. Pollen morphology does not confirm a close relationship between <em>S. aria</em> and <em>S. intermedia</em>. It is possible to distinguished pollen grains of all the analysed native species. However, there are no important differences in pollen morphology between the subspecies of <em>Sorbus aucuparia</em>. The key for Polish <em>Sorbus</em> species, based on pollen grain characters is given too.

scholarly journals Use of Pollen Morphology Traits for Identifying Species of Centaurea L. Asteraceae in Kurdistan-Iraq

2020 ◽  
Vol 8 (4) ◽  
pp. 131-138
Author(s):  
Rizgar Y. Ismail ◽  
Saleem E. Shahbaz
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Principal Component ◽  
Polar View ◽  
Pollen Grain ◽  
Medium Size ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
Total Variability ◽  
Pollen Shape

The polleniferous material of Centaurea taxa grow in Kurdistan-Iraq was collected. Samples were tested using scanning electron microscopy (SEM) and light microscopy (LM). In the polar view, pollen grain outlines are mostly triangular or triangular-circular. In equatorial view, the elliptic shape is common, while circulars or semicircular shapes are rare. The exine sculpture is scabrate, and the tectum is perforate. Number of sculpture microspinules/ µm², and their heights together with the number of wall perforations provide significant characters for taxa delimitation. Pollen grains of all species are of medium size, very few of some species pollen are larger or smaller in size. Species producing subprolate pollen shape is common, while oblate spheroidal and Prolate spheroidal shapes are less common. The principal component analysis and the discriminant analysis can recognize 65.478% and 87.54% of the total variability respectively; moreover, the discriminant function can correctly classify 125 or 78.125% out of 160 observations for pollen traits. 

scholarly journals SEM observations of pollen grains, fruits and seeds of the Pieniny Mountains (South Poland) endemic species Erysimum pieninicum (Zapał.) Pawł. (Brassicaceae)

2011 ◽  
Vol 76 (2) ◽  
pp. 127-132 ◽  
Author(s):  
Irmina Maciejewska ◽  
Leszek Bednorz ◽  
Toshiyuki Fujiki
Keyword(s):  
Seed Coat ◽  
Endemic Species ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Internal Surface

<em>Erysimum pieninicum</em> (Zapał.) Pawł. (<em>Brassicaceae</em>) is an endemic species, growing only in Pieniny Mts. The aim of the presented work was to investigate its pollen morphology as well as its ultrastructural traits of pericarp and seed-coat. Pollen grains of this species were of small size, 3-zonocolpate with reticulate ornamentation. The external siliqua surface was of rugose sculpture, covered by numerous, stellate hairs. The internal surface of the fruit was naked and characterized by striate sculpture. The seed-coat ornamentation of <em>E. pieninicum</em> was of blister type.

scholarly journals Pollinic characterization of Raulinoa echinata R. S. Cowan (Rutaceae), Dyckia brevifolia Baker and Dyckia ibiramensis Reitz (Bromeliaceae), reophyte and saxicolous endemic species of river Itajaí-Açu, Santa Catarina, Brazil

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Denise Monique Dubet da Silva Mouga ◽  
Bruna Tereza Possamai ◽  
Enderlei Dec ◽  
Sílvio Murilo Cristóvão Da Silva
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Endemic Species ◽  
Pollen Grains ◽  
Bilateral Symmetry ◽  
Radial Symmetry ◽  
Medium Size ◽  
Fresh Pollen ◽  
Scanning Electron

Aiming to contribute with elements about the reproduction of the reophytes Raulinoa echinata, Dyckia brevifolia and Dyckia ibiramensis as well as to support their taxonomic definition,  their pollinic characterization was carried out. Fresh pollen grains were prepared by acetolysis. Observations occurred on a light microscope and a scanning electron microscope. Measures were done of the polar (P) and equatorial diameters (E) and thickness of the exine and are expressed as means in micrometers. R. echinata: monad; radial symmetry; medium size; P=49,07±3,33; E=33,95±3,94; prolate (ratio P / E); ambitus subtriangular; 3-aperturated with long colpi and lalongate endoapertures; exine striated with  thickness 1.54 ± 0,32. Dyckia brevifolia: monad; bilateral symmetry; medium size; P=25,16 ± 3,02; E=43,16±3,50 x 28,12±3,26; ambitus elliptical; monoaperturated with one long sulcus; exine reticulate with thickness 1,66 ± 0,31. D. ibiramensis: monad; bilateral symmetry; medium size; P=29,54±3,17; E=42,36±3,60 x 28,43±3,38; ambitus elliptical; monoaperturated with one long sulcus; exine reticulate with thickness 1,73 ± 0,28. Data obtained can allow to verify hydrophily for the three species as well as suggest Raulinoa proximity with Metrodorea and Esenbeckia (both Pilocarpinae) and confirm D. brevifolia e D. ibiramensis to Pitcairnioideae. 

scholarly journals Pollen morphology of the genus Rubus L. I. introductory studies on the european representatives of the subgenus Rubus L.

2014 ◽  
Vol 64 (2) ◽  
pp. 187-203 ◽  
Author(s):  
Anna Tomlik-Wyremblewska
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Sem Observation ◽  
Internal Part ◽  
Rubus Species ◽  
First Time ◽  
Shape And Size

The results of pollen morphology studies of 9 European Rubus species are presented in this paper (<i>R. apricus</i>, <i>R. armeniacus</i>, <i>R. divaricatus</i>, <i>R. fabrimontanus</i>, <i>R. gracilis</i>, <i>R. hirtus</i>, <i>R. laciniatus</i>, <i>R. nessensis</i> and <i>R. pedemontanus</i>). The examination is made by SEM observation for first time and also by LM. All examined pollen grains are small, isopolar and tricolporate. All grains, except for <i>R. armeniacus</i> and <i>R. gracilis</i> tend to have very long colpi. Equatorial bridges constricted, make the pores unvisible in most of the species. <i>R. apricus</i>, <i>R. armeniacus</i>, <i>R. gracilis</i> and <i>R. pedemontanus</i> have the equatorial bridges stretched therefore the dark whole was only the trace of existing endoaperture. The sculpture of the grain confirms striate pattern and the orientation of the muri indicates the differences between the species; <i>R. pedemontanus</i> and <i>R. fabrimontanus</i> appear to have long, compact muri, parallelly orientated to the colpus; <i>R. nessensis</i> appears muri meandrically orientated, reminiscent of "finger prints" pattern with distinct thickenings in the place of connection and numerous narrow grooves with perforations. <i>R. hirtus</i> appears to have striate-scabrate sculpture because of granular elements (punctae) dispersed between the muri on all over the grain like the internal part of the exine. Shape and size turned out to be a poor criterion for identifying species.

scholarly journals Morfología polínica de especies de la selva mediana subperennifolia en la cuenca del río Candelaria, Campeche

2019 ◽  
Vol 84 ◽  
pp. 83
Author(s):  
Yajaira De los Angeles Sánchez Dzib ◽  
Susana Sosa Nájera ◽  
María Del Socorro Lozano García
Keyword(s):  
Tropical Forest ◽  
River Basin ◽  
Endemic Species ◽  
Pollen Morphology ◽  
Yucatan Peninsula ◽  
Pollen Grains ◽  
La Selva ◽  
Exine Ornamentation ◽  
Del Rio ◽  
Calliandra Houstoniana

Detailed pollen morphology of 81 species belonging to the semi-evergreen tropical forest of the Candelaria River Basin, Campeche is presented. Pollen morphology of some endemic species from the Yucatan peninsula semi-evergree tropical forest are presented: <em>Diospyros cuneata, Croton icche, Acacia gaumeri, Bauhinia erithrocalyx, Calliandra houstoniana, Lonchocarpus longistylus, Trichilia minutifl ora, Coccoloba refl exifl ora, Neomillispaughia emarginata, Randia longiloba, Talisia fl oresii, Thinouia paucidentata and Pouteria amygdalina </em>. Exine ornamentation, structure, apertures, form and size of the pollen grains are included in the description. Details of ornamentation and apertures are illustrated.

scholarly journals Palynomorphological study of primrose (Primula vulgaris Huds.) grown in natural reserve Obedska bara (Serbia)

2010 ◽  
Vol 55 (3) ◽  
pp. 227-234
Author(s):  
Marina Macukanovic-Jocic ◽  
Dragana Rancic ◽  
Zora Dajic-Stevanovic
Keyword(s):  
Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Medium Size ◽  
Morphometric Characteristics ◽  
Primula Vulgaris ◽  
Natural Reserve ◽  
Exine Ornamentation ◽  
Scanning Electron ◽  
Shape And Size

The pollen morphology of primrose (Primula vulgaris, fam. Primulaceae) has been investigated using light and scanning electron microscopy to contribute to melissopalynological studies of honeys originating from the native apiflora. Palynomorphological investigation included the examination of pollen symmetry, polarity, ornamentation, aperturation, shape and size. The pollen grains are isopolar, radially symmetric and shed as monads. The exine ornamentation is reticulate. Analysis of pollen morphometric characteristics revealed that grains are small to medium size and prolate in shape. Given the aperturation, the number of colpi was mostly variable among individuals with a different type of flower ranging from 6 to 9.

Sign in / Sign up

Export Citation Format

Share Document