Influence of exogenously applied paclobutrazol on some physiological traits and growth of Stevia rebaudiana under in vitro drought stress

Biologia ◽  
2013 ◽  
Vol 68 (3) ◽  
Author(s):  
Shokoofeh Hajihashemi ◽  
Ali Ehsanpour

AbstractThis investigation was carried on to find out the changes occurred in Stevia rebaudiana in response to paclobutrazol (PBZ; 0–4 mg L−1) treatment and drought stress. Polyethylene glycol (PEG; 0–6 % w/v) was used to stimulate drought stress. Drought stress reduced fresh and dry weight, water content, chlorophylls, carotenoids, anthocyanins, water soluble carbohydrates, reducing sugar and proline amounts. Electrolyte leakage, MDA, α-tocopherol and glycine betaine contents increased in drought-stressed plants. The activity of P5CS and PDH enzymes and protein content showed no significant changes under drought stress. PBZ (with or without PEG) treatments decreased fresh and dry weight and water content. In PBZ-treated plants, less pigments was damaged by drought stress. PBZ treatment reduced the negative effect of drought stress on lipid peroxidation which resulted in lower electrolyte leakage and MDA content, compared to the same PEG level without PBZ. PBZ (with or without PEG) treatments increased glycine betaine, α-tocopherol, proline and protein contents. The amount of water soluble carbohydrates, reducing sugar and activity of P5CS and PDH were not affected by PBZ treatments. SDS-PAGE analysis revealed that drought stress increased a 25 kD protein with a critical function in plant development under stresses. According to the results, PEG provoked a severe drought stress in S. rebaudiana that could partly be restored by PBZ treatment.

2020 ◽  
Vol 155 ◽  
pp. 262-270 ◽  
Author(s):  
Yuan Liu ◽  
Peipei Zhang ◽  
Mengfei Li ◽  
Lei Chang ◽  
Hongbo Cheng ◽  
...  

2021 ◽  
Author(s):  
Sriramachandrasekharan Malayappa venkataraman ◽  
Gokula Priya Natarajan ◽  
Manivannan Rengarajan ◽  
Prakash Muthu Arjuna Samy

Abstract Purpose Maize is grown under a wide spectrum of soil and climatic conditions. Maize is moderately sensitive to salt stress and response of maize under alkaline stress is scanty. Further, silicon is known to protect crops from abiotic stress. Hence, a pot experiment was conducted to study the mechanism on the effect of silicon on maize grown in alkaline stress Methods Maize CO 8 was grown in soil treated with four levels of alkaline stress (0, 25, 50, 75 mM) created through addition of sodium carbonate and three levels of silicon (0,100 and 150 kg ha− 1) applied to root through sodium meta silicate. The experiment was conducted in factorial CRD with three replications. Results Alkaline stress at graded levels caused reduction in growth (5 to 16%), dry weight (28 to 59%) and relative water content (5 to 23%). But electrolyte leakage (6 to 49%), proline (26 to 62%), phenol (8 to 44%), protein (6 to 19%), anti-oxidant systems viz., peroxidase (30 to 52%), SOD (4 to 16%) and catalase activities (32 to 127%) increased with increase in alkaline stress level. Soil application of silicon at different levels improved growth ( 5 to 10%) and dry weight (17 to 30%) of maize, relative water content (6 to 12%) and antioxidant enzymes ( 25 to 52%), water soluble protein ( 7 to10%), phenol ( 10 to 18%) while reduced electrolyte leakage( 15 to 25%) and proline( 17 to 29%). Conclusions It is evident from the study that root supplementation of silicon improved the growth and dry weight of maize crop grown in alkaline stress soil through its action on antioxidant systems and maximum effect was evident with 150 kg Si/ha


1999 ◽  
Vol 124 (6) ◽  
pp. 581-586 ◽  
Author(s):  
Arthur D. Wall ◽  
Marisa M. Wall ◽  
Joe N. Corgan

Onions (Allium cepa L.) with ≥18% bulb dry weight are dehydrated and used for spices and food ingredients. Bulb weight characteristics and water-soluble carbohydrates (WSC) of two commercial dehydrator cultivars, GS02 and GS04, and a breeding population, NM9335, were studied before and after maturity to observe phenotypic traits that may be useful for selection during breeding programs, and to study dehydrator onion carbohydrate physiology. At maturity, NM9335, GS02, and GS04 bulbs had 11.9 ± 0.33%, 18.6 ± 0.27%, and 19.4 ± 0.40% dry weight, respectively. Mature GS04 plants had 76.5 ± 0.01% of whole plant dry weight in bulbs, which is an extraordinarily high crop harvest index. NM9335 bulbs had higher fresh (hydrated) weight than bulbs of GS04 and GS02, but bulbs in all populations accumulated similar amounts of dry weight. Bulb percent dry weight before maturity did not indicate percent dry weight at maturity in the high-solids commercial onion cultivars. Bulb percent dry weight declined slightly after maturity in all populations. Glucose, fructose, and sucrose were relatively low, and fructans with degree of polymerization ≥6 were relatively high in GS04, but the converse was observed in NM9335. Relative amounts of GSO4 bulb fructan increased sequentially, in order of rank, from DP4 to DP6, but the converse was observed for NM9335.


2022 ◽  
Vol 13 (SPL) ◽  
Author(s):  
Arpit Gaur ◽  
Deepti Sharma ◽  
Sonia Sheoran ◽  
Sulekha Chahal ◽  
Kaveri Chaudhary ◽  
...  

1993 ◽  
Vol 118 (6) ◽  
pp. 766-770 ◽  
Author(s):  
W.M. Randle ◽  
M.L. Bussard

Sixteen short-day onion (Allium cepa L.) cultivars with high (4.0 meq·liter-1) and low S (0.1 meq·liter-1) fertility were evaluated for several characteristics associated with bulb flavor. Sulfur levels interacted with cultivars in influencing bulb pungency and concentrations of S and individual sugars, except for fructose. Enzymatically formed pyruvic acid correlated poorly with bulb S concentration, which suggests differential partitioning of S into flavor and nonflavor compounds among cultivars. Bulb percent dry weight correlated negatively with bulb S concentration. Since poor correlations were found between enzymatically formed pyruvic acid and water-soluble carbohydrates, we postulate that pungency and sweetness function independently in bulbs of fresh-market short-day onion cultivars.


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