Nanopotassium, Nanosilicon, and Biochar Applications Improve Potato Salt Tolerance by Modulating Photosynthesis, Water Status, and Biochemical Constituents

Salinity is one of the main environmental stresses, and it affects potato growth and productivity in arid and semiarid regions by disturbing physiological process, such as the photosynthesis rate, the absorption of essential nutrients and water, plant hormonal functions, and vital metabolic pathways. Few studies are available on the application of combined nanomaterials to mitigate salinity stress on potato plants (Solanum tuberosum L. cv. Diamont). In order to assess the effects of the sole or combined application of silicon (Si) and potassium (K) nanoparticles and biochar (Bc) on the agro-physiological properties and biochemical constituents of potato plants grown in saline soil, two open-field experiments were executed on a randomized complete block design (RCBD), with five replicates. The results show that the biochar application and nanoelements (n-K and n-Si) significantly improved the plant heights, the fresh and dry plant biomasses, the numbers of stems/plant, the leaf relative water content, the leaf chlorophyll content, the photosynthetic rate (Pn), the leaf stomatal conductance (Gc), and the tuber yields, compared to the untreated potato plants (CT). Moreover, the nanoelements and biochar improved the content of the endogenous elements of the plant tissues (N, P, K, Mg, Fe, Mn, and B), the leaf proline, and the leaf gibberellic acid (GA3), in addition to reducing the leaf abscisic acid content (ABA), the activity of catalase (CAT), and the peroxidase (POD) and polyphenol oxidase (PPO) in the leaves of salt-stressed potato plants. The combined treatment achieved maximum plant growth parameters, physiological parameters, and nutrient concentrations, and minimum transpiration rates (Tr), leaf abscisic acid content (ABA), and activities of the leaf antioxidant enzymes (CAT, POD, and PPO). Furthermore, the combined treatment also showed the highest tuber yield and tuber quality, including the contents of carbohydrates, proteins, and the endogenous nutrients of the tuber tissues (N, P, and K), and the lowest starch content. Moreover, Pearson’s correlation showed that the plant growth and the tuber yields of potato plants significantly and positively correlated with the photosynthesis rate, the internal CO2 concentration, the relative water content, the proline, the chlorophyll content, and the GA3, and that they were negatively correlated with the leaf Na content, PPO, CAT, ABA, MDA, and Tr. It might be concluded that nanoelement (n-K and n-Si) and biochar applications are a promising method to enhance the plant growth and crop productivity of potato plants grown under salinity conditions.

Influence of drought stress on growth, biochemical changes and leaf gas exchange of strawberry (Fragaria × ananassa Duch.) in Indonesia

<abstract> <p>Drought stress is one of the challenges that can affect the growth and the quality of strawberry. The study aims to determine the growth, biochemical changes and leaf gas exchange of three strawberry cultivars under drought stress. This study was conducted in a glasshouse at Indonesian Citrus and Subtropical Fruits Research Institute, Indonesia, from July-November 2018. The experiment was arranged in a factorial randomized completely block design (RCBD) with three replications and four water deficit (WD) levels [100% field capacity (FC)/well-watered), 75% of FC (mild WD), 50% of FC (moderate WD), and 25% of FC (severe WD)] for three strawberry cultivars (Earlibrite, California and Sweet Charlie). The results showed that total chlorophyll and anthocyanin contents (p ≤ 0.05) were influenced by the interaction effects of cultivars and water deficit. Whereas other parameters such as plant growth, transpiration rate (<italic>E</italic>), net photosynthesis (<italic>A</italic>), stomatal conductance (<italic>gs</italic>), leaf relative water content (LRWC), flowers and fruits numbers, proline content, length, diameter, weight and total soluble solid (TSS) of fruit were affected by water deficit. <italic>A</italic> had positive significant correlation with plant height (r = 0.808), leaf area (r = 0.777), fruit length (r = 0.906), fruit diameter (r = 0.889) and fruit weight (r = 0.891). Based on the results, cultivars affected LRWC, and also number of flowers and fruits of the strawberry. This study showed that water deficit decreased plant growth, chlorophyll content, leaf gas exchange, leaf relative water content, length, diameter and weight of fruit but enhanced TSS, anthocyanin, MDA, and proline contents. Increased anthocyanin and proline contents are mechanisms for protecting plants against the effects of water stress. California strawberry had the highest numbers of flowers and fruits, and also anthocyanin content. Hence, this cultivar is recommended to be planted under drought stress conditions. Among all water stress treatments, 75% of FC had the best results to optimize water utilization on the strawberry plants.</p> </abstract>

Application of Cyanobacteria (Roholtiella sp.) Liquid Extract for the Alleviation of Salt Stress in Bell Pepper (Capsicum annuum L.) Plants Grown in a Soilless System

Salinity is one of the abiotic stresses that affect crop growth and productivity in arid and semi-arid regions. Unfortunately, there are few known methods to mitigate the deleterious impacts of salt stress on the development and yield of vegetable crops. Blue-green algae (cyanobacteria) are endowed with the potential to curb the negative impacts of salt stress as they are characterized by biostimulant properties. The present work aimed to investigate the effects of Roholtiella sp. as a foliar extract on the growth characteristics, physiological and biochemical responses of bell pepper (Capsicum annuum L.) plants under varying levels of salinity conditions. A soilless water experiment was carried out in a greenhouse where bell pepper seedlings were grown under five salt concentrations (0, 50, 200, 150, and 200 mM of NaCl). Growth characteristics, pigments content, relative water content, and antioxidant activity (CAT) were determined. Our results showed that growth parameters, relative water content (RWC), chlorophyll a & b concentrations under salinity conditions were negatively affected at the highest concentration (200 mM). Interestingly, the application of Roholtiella sp. foliar extract enhanced the plant growth characteristics as shoot length increased by 17.014%, fresh weight by 39.15%, dry and weight by 31.02%, at various salt treatments. Moreover, chlorophyll a and b increased significantly compared with seedlings sprayed with water. Similarly, RWC exhibited a significant increase (92.05%) compared with plants sprayed with water. In addition, antioxidants activities and accumulation of proline were improved in Roholtella sp. extract foliar sprayed seedlings compared to the plants foliar sprayed with water. Conclusively, at the expiration of our study, the Rohotiella sp. extract-treated plants were found to be more efficient in mitigating the deleterious effects caused by the salinity conditions which is an indication of an enhancement potential of tolerating salt-stressed plants when compared to the control group.

AIR POLLUTION TOLERANCE INDEX OF WHEAT AND RICE IN THE PROXIMITY OF GAS BASED POWER PLANT

The study aimed to identify the tolerance level of rice and wheat due to air pollutants around the gas-based power plant. Ten sites were selected around 10km radius of gas based power plant. Major air pollutants like NOx, SOx, ozone, and PM10 were monitored in ten sites during the growth of rice and wheat. The Air Quality Index (AQI) of villages falls mostly in the category of moderately polluted sites. Air Pollution Tolerance Index (APTI) is a tool applied for categorizing sensitive or tolerant plants towards air pollution and is calculated by using four biochemical parameters like total chlorophyll content, ascorbic acid, pH, and relative water content of rice and wheat. Results indicated that the pH of cell sap of both the crops was acidic to neutral pH (3.5-6.9) at polluted sites while neutral to slightly alkaline (7.0-7.9) at less polluted sites. Ascorbic acid content was high at polluted sites since ascorbic acid shows a defense mechanism against air pollution. Chlorophyll content (up to 0.61mg/g) and relative water content have shown a significant decrease at most polluted sites. As per APTI values (APTI<11), both the crops were sensitive to air pollution in the selected area. APTI might be beneficial in the selection of crop species in the polluted area which shows that a higher concentration of air pollutants can damage crops severely. APTI was found to be positively correlated with pH, total chlorophyll content, and relative water content and negatively correlated with ascorbic acid.

Transcriptome analysis of Kentucky bluegrass subject to drought and ethephon treatment

Kentucky bluegrass (Poa pratensis L.) is an excellent cool-season turfgrass utilized widely in Northern China. However, turf quality of Kentucky bluegrass declines significantly due to drought. Ethephon seeds-soaking treatment has been proved to effectively improve the drought tolerance of Kentucky bluegrass seedlings. In order to investigate the effect of ethephon leaf-spraying method on drought tolerance of Kentucky bluegrass and understand the underlying mechanism, Kentucky bluegrass plants sprayed with and without ethephon are subjected to either drought or well watered treatments. The relative water content and malondialdehyde conent were measured. Meanwhile, samples were sequenced through Illumina. Results showed that ethephon could improve the drought tolerance of Kentucky bluegrass by elevating relative water content and decreasing malondialdehyde content under drought. Transcriptome analysis showed that 58.43% transcripts (254,331 out of 435,250) were detected as unigenes. A total of 9.69% (24,643 out of 254,331) unigenes were identified as differentially expressed genes in one or more of the pairwise comparisons. Differentially expressed genes due to drought stress with or without ethephon pre-treatment showed that ethephon application affected genes associated with plant hormone, signal transduction pathway and plant defense, protein degradation and stabilization, transportation and osmosis, antioxidant system and the glyoxalase pathway, cell wall and cuticular wax, fatty acid unsaturation and photosynthesis. This study provides a theoretical basis for revealing the mechanism for how ethephon regulates drought response and improves drought tolerance of Kentucky bluegrass.

Mitigation of Drought Damages by Exogenous Chitosan and Yeast Extract with Modulating the Photosynthetic Pigments, Antioxidant Defense System and Improving the Productivity of Garlic Plants

Garlic is an important vegetable in terms of its economic value and also as a medicinal plant. In this study, chitosan (300 mM) and yeast extract (8 g/L) were used individually or in combination to improve the yields of garlic plants under drought conditions (i.e., 75% and 50% of the water they would normally receive from irrigation) for two seasons. Significant decreases in numbers of leaves per plant and plant height, plant dry weight, relative water content, and chlorophyll a and b concentrations were found in stressed garlic plants in both seasons. The greatest reductions in these characters were recorded in plants that received only 50% of the normal irrigation in both seasons. Levels of hydrogen peroxide, products of lipid peroxidation such as malondialdehyde, and superoxide, as well as percentages of electrolyte leakage, were elevated considerably and were signals of oxidative damage. The application of the yeast extract (8 g/L) or chitosan (300 mM) individually or in combination led to a remarkable increase in the most studied characters of the stressed garlic plants. The combination of yeast extract (8 g/L) plus chitosan (300 mM) led to increase plant height (44%), ascorbic acid levels (30.2%), and relative water content (36.8%), as well as the chlorophyll a (50.7%) and b concentrations (79%), regulated the proline content and levels of antioxidant enzymes in stressed garlic plants that received 75% of the normal irrigation, and this decreased the signs of oxidative stress (i.e., percentage of electrolyte leakage and levels of malondialdehyde, hydrogen peroxide, and superoxide).

Effect of weather variability on growth and yield of wheat crop under semi-arid region of India

For generating different weather conditions during various phenological stages, experiments were conducted on two varieties of wheat (HD-2967 and HD-3086) sown on three different dates at the research farm of IARI, New Delhi during rabi 2015-16 and 2016-17. Soil temperature, soil moisture, leaf area index, biomass, chlorophyll content, radiation interceptions were measured during different crop growth stages. Number of days taken for each phenological stage was observed and thermal time for different phenological stages were calculated. Results showed that first sown crop had higher value of crop growth parameters and yield as compared to second and third sown crop.HD-3086 had higher value of LAI, biomass and yield than HD-2967. Grain yield had significant positive correlation with growing degree days during grain filling stage. Soil temperature measured at 2.21 PMat 5, 10, 15, 20 cm depth had 1-5°C lower value than the air temperature. Soil moisture measured at 0-15, 15-30, 30-45 and 45-60 cm depths had slightly higher soil moisture for HD-3086 as compared to corresponding value in HD-2967 during emergence, flowering and grain filling stages. Percentage relative water content in HD-2967 was found to be higher in first sown crop followed by second and third sown crop. However, in HD-3086, percentage relative water content was found to be higher in first sown crop followed by third and second sown crop. Grain yield had significant positive correlation with relative water content during different phenological stages. HD-3086hadhigherradiation use efficiency as compared to HD-2967 in all weather conditions.

The effect of biological fertilizer application on interest amount and relative water content in rehabilitation effort of old cacao

Purpose This study was to examine the effect of the application of Trichoderma asperellum and Azotobacter chroococcum on the condition of cacao leaves (RWC and LMA) and their relationship to the interest amount formed, after treatment Inarching grafting in the rehabilitation of old cacao plants from side grafting. The method used in this study was Split Plot Design, with 2 factors, namely the use of T. asperellum and A. chroococcum which were repeated 3 times each, and using Duncan’s New Multiple Range Test (DNMRT). The best results obtained were the relative water content of the leaves 98.43%; LMA 225.05 mg.mm−2, which produced an average number of 62 flowers every tree, with bacteria A.chroococcum and fungus T.asperellum applied twice each using the inarching grafting method which was carried out at the time of the appearance of the flush.