scholarly journals Analysis of IL-2 Production in Hookworm Infected Hamsters Using Generated Polyclonal Antibodies

2012 ◽  
Vol 56 (1) ◽  
pp. 37-42
Author(s):  
Ewa Długosz ◽  
Jarosław Cendrowski ◽  
Piotr Bąska ◽  
Anna Siwińska ◽  
Halina Wędrychowicz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lymph Nodes ◽  
Cell Cultures ◽  
Polyclonal Antibodies ◽  
Mesenteric Lymph Nodes ◽  
Ancylostoma Ceylanicum ◽  
Specific Antibodies ◽  
Mesenteric Lymph ◽  
Race Pcr

Abstract The aim of the study was cloning and analysis of the entire coding sequence of hamster IL-2 by the method of RACE-PCR, its expression in Escherichia coli cells, and production of IL-2 specific antibodies. These antibodies were used to determine in vitro IL-2 production by cells derived from the spleen and mesenteric lymph nodes of Ancylostoma ceylanicum infected hamsters. The highest concentration of IL-2 was noted in supernatants from cell cultures coming from the oldest, most resistant hamsters.

Inhibition of Escherichia coli Translocation from the Gastrointestinal Tract by Normal Cecal Flora in Gnotobiotic or Antibiotic-Decontaminated Mice

1980 ◽  
Vol 29 (3) ◽  
pp. 1073-1081
Author(s):  
Rodney D. Berg
Keyword(s):  
Escherichia Coli ◽  
Gastrointestinal Tract ◽  
Lymph Nodes ◽  
Bacterial Flora ◽  
Specific Pathogen Free ◽  
Mesenteric Lymph Nodes ◽  
E Coli ◽  
Mesenteric Lymph ◽  
Specific Pathogen ◽  
Gnotobiotic Mice

Escherichia coli C25 maintained population levels of 10 9 to 10 10 per g of cecum and translocated to 100% of the middle mesenteric lymph nodes in gnotobiotic mice monoassociated with E. coli C25. Intragastric inoculation of these mice with the cecal contents from specific-pathogen-free mice reduced the population levels of E. coli C25 to 10 6 per g of cecum and completely inhibited translocation to the mesenteric lymph nodes. Intragastric inoculation with heat-treated, Formalintreated, or filtered cecal contents did not reduce the population levels of E. coli C25 or reduce the incidence of translocation of E. coli C25 to the mesenteric lymph nodes. Thus, viable bacteria apparently are required in the cecal contents inocula to reduce the population levels and the incidence of translocation of E. coli C25. Treatment with streptomycin plus bacitracin decreased the anaerobic bacterial levels in these gnotobiotic mice, allowing increased population levels of E. coli C25 and increased translocation to the mesenteric lymph nodes. E. coli C25 also translocated to the mesenteric lymph nodes of specific-pathogen-free mice treated with streptomycin and bacitracin before colonization with E. coli C25. The high cecal population levels of E. coli C25 in these antibiotic-decontaminated specific-pathogen-free mice apparently overwhelm any barrier to translocation exerted by the immunologically developed lamina propria of the specific-pathogen-free mice. Inoculation of gnotobiotic mice with a cecal flora also reduced the population levels of an indigenous strain of E. coli with a concomitant inhibition of translocation of the indigenous E. coli to the mesenteric lymph nodes. Thus, bacterial antagonism of the gastrointestinal population levels of certain indigenous bacteria, such as E. coli , by other members of the normal bacterial flora appears to be an important defense mechanism confining bacteria to the gastrointestinal tract.

STUDY OF LYMPHOCTYE INTERACTION IN HUMAN MESENTERIC LYMPH NODES IN VITRO

1984 ◽  
Vol 62 (5) ◽  
pp. 531-537
Author(s):  
AW Cripps ◽  
RL Clancy ◽  
H Chipchase ◽  
EJ Hennessy
Keyword(s):  
Lymph Nodes ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph

Detection of Verocytotoxin-Producing Escherichia coli Serogroups O157 and O26 in the Cecal Content and Lymphatic Tissue of Cattle at Slaughter in Italy

2007 ◽  
Vol 70 (6) ◽  
pp. 1493-1497 ◽  
Author(s):  
SILVIA BONARDI ◽  
EMANUELA FONI ◽  
CHIARA CHIAPPONI ◽  
ALESSANDRA SALSI ◽  
FRANCO BRINDANI
Keyword(s):  
Escherichia Coli ◽  
Lymph Nodes ◽  
Foodborne Pathogen ◽  
Immunomagnetic Separation ◽  
Feedlot Cattle ◽  
Cecal Content ◽  
Lymphatic Tissue ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Multiplex Pcr Assay

Verocytotoxin-producing Escherichia coli (VTEC) has emerged as a foodborne pathogen that can cause severe and potentially fatal illnesses, such as hemorrhagic colitis or the hemolytic uremic syndrome. In this study, 182 cattle at slaughter (119 dairy cows and 63 feedlot cattle) were randomly selected and tested for the presence of VTEC serogroups O26, O103, O111, O145, and O157 in their cecal content and lymphatic tissue (tonsils or mesenteric lymph nodes). A total of 364 samples were evaluated with an immunomagnetic separation technique followed by slide agglutination. Presumptive VTEC O26, O103, O111, O145, and O157 isolates were tested by Vero cell assay for verocytotoxin production and by multiplex PCR assay for the detection of vtx1, vtx2, eae, and E-hlyA genes. VTEC O157 was detected in 6 (3.3%) of 182 animals, and VTEC O26 was detected in 1 (0.5%) of 182 animals. No VTEC O103, VTEC O111, or VTEC O145 isolates were found in cattle feces, but one VTEC O91:H− vtx2+, eae−, E-hlyA+ strain nonspecifically cross-reacted with the VTEC O103 type. The prevalence of VTEC O157 in the lymphatic tissue of cattle was 1.1% in both tonsils (1 of 93 samples) and mesenteric lymph nodes (1 of 89 samples). Lymphatic tissue contamination was observed only in VTEC O157 intestinal carriers; two (33.3%) of six fecal carriers were simultaneously VTEC O157 lymphatic carriers. This finding suggests that VTEC O157 contamination of meat does not necessarily come from feces or the environment. No other VTEC serogroups were detected in the lymphatic tissue of slaughtered cattle.

Bulking fibre prevents translocation to mesenteric lymph nodes of an efficiently translocating Escherichia coli strain in rats

1998 ◽  
Vol 17 (4) ◽  
pp. 185-190 ◽  
Author(s):  
C.-G. Nettelbladt ◽  
M. Katouli ◽  
T. Bark ◽  
T. Svenberg ◽  
R. Möllby ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lymph Nodes ◽  
Coli Strain ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph

scholarly journals Endothelium-dependent hyperpolarization-mediated relaxation pathway in bovine mesenteric lymph nodes

2019 ◽  
Vol 484 (5) ◽  
pp. 645-648
Author(s):  
G. I. Lobov ◽  
D. P. Dvoretskii
Keyword(s):  
Lymph Nodes ◽  
Physiological Saline ◽  
Relaxation Mechanism ◽  
Mesenteric Lymph Nodes ◽  
Tetraethylammonium Chloride ◽  
K Channels ◽  
Mesenteric Lymph ◽  
Lymph Node Capsule ◽  
Endothelium Dependent Relaxation

In vitro, endothelium-dependent relaxation mechanisms of smooth muscle cells of the bovine mesenteric lymph node capsule have been studied. The addition of L-NAME and indomethacin to physiological saline inhibited the production of endothelium NO and prostacyclin. In this solution, tetraethylammonium chloride and TRAM-34 increased the tone of the precontracted lymph nodes. Thus, in bovine mesenteric lymph nodes there is an relaxation mechanism mediated by endothelial hyperpolarization, realized by activating Ca2+-dependent K+-channels of large- and intermediate conductance.

Effects of Inhaled Hexachlorobenzene Aerosols on Rat Pulmonary Host Defenses

1989 ◽  
Vol 5 (3) ◽  
pp. 451-461 ◽  
Author(s):  
Robert L. Sherwood ◽  
Peter T. Thomas ◽  
William J. O'shea ◽  
Jeannie N. Bradof ◽  
Helen V. Ratajczak ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
B Cell ◽  
Bactericidal Activity ◽  
Peritoneal Macrophages ◽  
Sprague Dawley ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Sprague Dawley Rats ◽  
Cell Mitogen

Pulmonary bactericidal activity, macrophage phagocytic activity, alveolar macrophage (AM) enzyme activity, and T- and B-cell mitogenesis of lymphocytes from lung associated lymph nodes (LALN) or mesenteric lymph nodes (MESLN) were assessed in Sprague-Dawley rats exposed 4 hr/d, 4 days/wk for 1, 4, or 16 days to hexachlorobenzene (HCB) aerosols. Pulmonary bactericidal activity was depressed after 1 or 4 but not 16 exposures to 35 mg/m3 of HCB. AM phagocytosis of 51Cr-RBC in vitro was increased after 4 but not 1 or 16 exposures to HCB, and no effect was observed in peritoneal macrophages. HCB significantly enhanced mitogenesis in MESLN to the B-cell mitogen Salmonella typhimurium lipopolysaccharide (STM) after 4 exposures; LALN STM mitogenesis and LALN and MESLN mitogenesis to phytohemagglutinin (PHA) were not affected. After 16 exposures, however, the PHA responses in LALN and MESLN were significantly increased and decreased, respectively.

scholarly journals AvrA effector protein of Salmonella enterica serovar Enteritidis is expressed and translocated in mesenteric lymph nodes at late stages of infection in mice

Microbiology ◽  
2014 ◽  
Vol 160 (6) ◽  
pp. 1191-1199 ◽  
Author(s):  
Mónica N. Giacomodonato ◽  
Mariángeles Noto Llana ◽  
María del Rosario Aya Castañeda ◽  
Fernanda R. Buzzola ◽  
Sebastián H. Sarnacki ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Culture Conditions ◽  
Effector Protein ◽  
Post Inoculation ◽  
Mesenteric Lymph Nodes ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Major Health Problem ◽  
Mesenteric Lymph

Salmonellosis is a major health problem worldwide. Salmonella enterica serovar Enteritidis (S. Enteritidis) has been a primary cause of Salmonella outbreaks in many countries. AvrA is an SPI-1 effector protein involved in the enteritis pathway, with critical roles in inhibiting inflammation and apoptosis. In this work, we constructed an AvrA-FLAG-tagged strain of S. Enteritidis to analyse the expression profile of AvrA in vitro, in cell culture and in vivo. AvrA expression and secretion were observed in vitro under culture conditions that mimicked intestinal and intracellular environments. In agreement, bacteria isolated from infected cell monolayers expressed and translocated AvrA for at least 24 h post-inoculation. For in vivo experiments, BALB/c mice were inoculated by the natural route of infection with the AvrA-FLAG strain. Infecting bacteria and infected cells were recovered from mesenteric lymph nodes (MLN). Our results showed that AvrA continues to be synthesized in vivo up to day 8 post-inoculation. Moreover, AvrA translocation was detected in the cytosol of cells isolated from MLN 8 days after infection. Interestingly, we observed that AvrA is secreted by both type three secretion system (T3SS)-1 and T3SS-2. In summary, these findings indicate that AvrA expression is not constrained to the initial host–bacteria encounter in the intestinal environment as defined previously. The AvrA effector may participate also in systemic S. Enteritidis infection.

scholarly journals Ability of intestinal Escherichia coli to survive within mesenteric lymph nodes.

1987 ◽  
Vol 55 (11) ◽  
pp. 2834-2837 ◽  
Author(s):  
C L Wells ◽  
M A Maddaus ◽  
R P Jechorek ◽  
R L Simmons
Keyword(s):  
Escherichia Coli ◽  
Lymph Nodes ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph

scholarly journals Detection of Extended- Spectrum Beta-Lactamase producing Escherichia coli from mesenteric lymph nodes of wild boars (Sus scrofa)

2019 ◽  
Vol 7 (4) ◽  
Author(s):  
Silvia Bonardi ◽  
Clotilde Silvia Cabassi ◽  
Simona Longhi ◽  
Federico Pia ◽  
Margherita Corradi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lymph Nodes ◽  
Sus Scrofa ◽  
Mesenteric Lymph Nodes ◽  
Wild Boars ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Mesenteric Lymph ◽  
Emilia Romagna Region ◽  
Amoxicillin Clavulanic Acid

Wild boars (Sus scrofa) are increasing in several European countries, including Italy. In areas with intensive animal farming, like the Italian Emilia-Romagna region, they are likely to be exposed to antimicrobialresistant (AMR) bacteria of livestock origin. In 2017-2018, 108 mesenteric lymph nodes samples were collected from 108 wild boars hunted in Parma province, Emilia-Romagna region, to be tested for ESBL- and carbapenemase-producing Escherichia coli. One isolate (WB-21L) out of 108 (0.9%) was phenotypically confirmed as ESBLproducing E. coli. The strain WB-21L was tested by PCR for the genes blaSHV, blaCTX-M, blaTEM, blaAmpC, blaKPC, blaNDM, blaVIM, blaIMP, blaOXA-48, blaSPM, blaBIC, blaSIM, blaDIM, blaGIM, blaAIM, resulting positive for TEM β-lactamase. Resistance to ampicillin, amoxicillin/clavulanic acid, streptomycin, sulfasomidine, tetracycline and trimethoprim confirmed the multi-resistance nature of the strain WB-21L. Nine E. coli isolates showed resistance to meropenem by the Kirby Bauer test but none of them showed Meropenem MIC values indicative of resistance. In conclusion, the present study shows the presence of ESBL E. coli in wild boars and the possible risk of transfer to game meat handlers and consumers. Future studies are needed to better evaluate the sources of AMR bacteria in wildlife.

Translocation of Escherichia coli from the gastrointestinal tract to the mesenteric lymph nodes in gnotobiotic mice receiving Escherichia coli vaccines before colonization

1980 ◽  
Vol 30 (3) ◽  
pp. 894-898
Author(s):  
R D Berg ◽  
A W Garlington
Keyword(s):  
Escherichia Coli ◽  
Lymph Nodes ◽  
Serum Antibody ◽  
Immunoglobulin A ◽  
Mesenteric Lymph Nodes ◽  
E Coli ◽  
Specific Serum ◽  
Mesenteric Lymph ◽  
Antibody Titers ◽  
Gnotobiotic Mice

Germfree mice were immunized orally or intraperitoneally for 6 weeks with heat-killed vaccines of indigenous Escherichia coli or nonindigenous E. coli O 127: B8 before colonization with these strains. The mice exhibited increases in specific serum antibodies and intestinal immunoglobulin A reacting with the E coli antigens. Prior immunization did not reduce the gastrointestinal population levels of the E. coli strains attained 3 and 7 days after colonization. Neither oral nor intraperitoneal immunization with the E. coli strains before colonization decreased the incidence of bacterial translocation to the mesenteric lymph nodes or reduced the number of viable E. coli cells per mesenteric lymph node. There also was no relation in individual mice between serum antibody titers and the numbers of viable E. coli cells translocating to the mesenteric lymph nodes. Thus, prior vaccination with E. coli in this study did not decrease the incidence or reduce the numbers of viable E. coli translocating to the mesenteric lymph nodes in gnotobiotic mice monoassociated with E. coli.

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