scholarly journals Screening of Antimicrobial Activity of Various Extracts of the Stem Justicia gendarussa

2019 ◽  
pp. 1-7
Author(s):  
Dhanapal Venkatachalam ◽  
Akhib Rahman ◽  
Basil Sunny ◽  
Jensy Jacob ◽  
Nikhil Kuriyan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Aqueous Extract ◽  
Antimicrobial Agents ◽  
Pathogenic Fungus ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Standard Drug ◽  
Antibacterial And Antifungal

Objective: The present study was designed to check in-vitro efficacy of Antibacterial and antifungal activity of aqueous and hexane extracts of stem of Justicia gendarussa against selected bacterial and fungal strains. Justicia gendarussa, belonging to the family Acanthaceae, commonly known as ‘‘vatham kolli’ was used in treatment of bronchitis, inflammation, eye diseases, ear ache, vaginal discharges, rheumatism, dysentery, eczema and jaundice. Methodology: Hexane and aqueous extracts of Justicia gendarussa was used for antimicrobial screening. Antibacterial activity was tested against pathogenic bacterial strains Eschericha coli and Staphylococcus aureus. Also antifungal activity was tested against one human pathogenic fungal strain i.e. Candida albicans. Antibacterial and antifungal activities of Justicia gendarussa extract was carried out by using disc diffusion method. Results: Aqueous extract of Justicia gendarussa showed the largest zone of inhibition (5mm) against Eschericha coli, at 50µgm/ml and 3mmzone of inhibition against S. aures, at 50µgm/ml. Hexane extract does not   showing activity against to human pathogenic E. coli and S. aures, at 50µgm/ml. Aqueous extract has shown more potent antibacterial activity against Eschericha coli. Aqueous extract was found to be more effective against human pathogenic fungus Candida albicuns than hexane extract and standard drug. The zone of inhibition of Aqueous extract was 11mm and the zone of inhibition of hexane extract was7mm and standard only 2mm.The phytochemical screening demonstrated the presence of different types of compounds like terpenoids, tannins, and flavonoids which may contribute for the anti-microbial action of this plant. Conclusions: These findings provide scientific evidence of traditional use of Justicia gendarussa and also indicate the potential of this plant for the development of antimicrobial agents.

scholarly journals Evaluation of Antimicrobial Activity of Sida cordifolia Leaf Extract

2019 ◽  
pp. 1-7 ◽  
Author(s):  
Dhanapal Venkatachalam ◽  
Samuel Thavamani ◽  
Aneesh C. Sebastian ◽  
V. B. Anju ◽  
Christy Mathew ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Pathogenic Fungus ◽  
Chloroform Extract ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Antifungal Activities ◽  
Antibacterial And Antifungal Activities ◽  
Sida Cordifolia ◽  
Antibacterial And Antifungal

Objective: The objective of the study was to evaluate in-vitro efficacy of antibacterial, and antifungal activities of chloroform extract of leaf of Sida cordifolia L. against some human pathogenic bacterial and fungal strains. S. cordifolia (Malvaceae) is used as anti-rheumatic, analgesic, antipyretic, anti-asthmatic, nasal decongestant, antiviral, laxative, diuretic, aphrodisiac, hypoglycemic, hepatoprotective and in the treatment of Parkinson disease. Methodology: Chloroform extract of S.cordifolia was used for antimicrobial screening. Antibacterial activity was tested against pathogenic bacterial strains Eschericha coli and Staphylococcus aureus. Also antifungal activity was tested against one human pathogenic fungal strain i.e. Candida albicans. Antibacterial and antifungal activities of S. cordifolia extract was carried out by using disc diffusion method. Results: Chloroform extract of S. cordifolia showed the largest zone of inhibition (10mm) against S. aureus at 50µgm/mL and no zone of inhibition against E.coli at 50µgm/ml. Chloroform extract has shown more potent antibacterial activity against S.aureus. Chloroform extract was found to be more effective against human pathogenic fungus C. albicuns. The zone of inhibition of chloroform extract was 10mm.The phytochemical screening demonstrated the presence of different types of compounds like alkaloids, tannins and phenolic compounds which may contribute for the anti-microbial action of this plant.  Conclusions: The results concludes that chloroform extract of S. cordifolia leaf possess antimicrobial activity.

scholarly journals An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4383
Author(s):  
Barbara Lapinska ◽  
Aleksandra Szram ◽  
Beata Zarzycka ◽  
Janina Grzegorczyk ◽  
Louis Hardan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Resin Composite ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

scholarly journals ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF SILVER NANOPARTICLES AGAINST NEISSERIA GONORRHOEAE AND CANDIDA ALBICANS

2020 ◽  
Vol 8 (6) ◽  
pp. 179-187
Author(s):  
Titik Taufikurohmah ◽  
Tasha Anandya Tantyani
Keyword(s):  
Antibacterial Activity ◽  
Candida Albicans ◽  
Antifungal Activity ◽  
Neisseria Gonorrhoeae ◽  
Diffusion Method ◽  
Antibacterial And Antifungal Activity ◽  
Disk Diffusion Method ◽  
Fungal Inhibition ◽  
Inhibition Response ◽  
Antibacterial And Antifungal

This Research on the antibacterial and antifungal activity of nanosilver against Neisseria gonorrhoeae and Candida albincas fungi has been carried out. The purpose of this study was to determine antibacterial activity of nanosilver against Neisseria gonorrhoeae and antifungal activity against Candida albincas. Synthesis Nanosilver uses bottom up method and characterized using UV-Vis Spectrophotometer. Nanosliver concentrations used were 30, 40, 50, and 60 ppm. Antibacterial and antifungal activity tests using disk diffusion method. Observations obtained in form of the presence or absence of clear zones formed around paper discs indicate the inhibition of nanosilver on microbial growth. The results of testing the antifungal activity of Candida albicans on nanosilver with concentrations of 30, 40, 50 and 60 ppm resulted in clear zones of 9.73 nm, 11.46 nm, 11.93 nm, and 13 nm with fungal inhibition response categories is medium and strong. The results antibacterial activity test of Neisseria gonorrhoeae on nanosilver with concentrations of 30, 40, 50 and 60 ppm did not show any clear zone around the disc, it showed that nanosilver in this study did not have antibacterial activity against Neisseria gonorrhoeae.

SYNTHESIS AND EVALUATION OF SOME SUBSTITUTED 2-AMINOTHIAZOLE DERIVATIVES FOR THEIR ANTITUBERCULAR, ANTIMICROBIAL & ANTIFUNGAL ACTIVITY

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (01) ◽  
pp. 24-32
Author(s):  
S. R. Pattan ◽  
◽  
S. H Kale ◽  
R. A. Mali ◽  
S. S. Dengale ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Antifungal Agents ◽  
Antitubercular Activity ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Standard Drug ◽  
E Coli ◽  
H Nmr ◽  
Antimicrobial And Antifungal Activity ◽  
Micro Organisms

Millions of people are affected by infectious diseases caused by micro-organisms. Further the widespread microbial resistance had renewed the interest in quest for new antitubercular, antimicrobial & antifungal agents. The present study deals with synthesis & evaluation of some substituted 2-aminothiazole derivatives for their antitubercular, antimicrobial and antifungal activity. 2-aminothiazole derivatives were synthesized by treating substituted acetophenones with thiourea in presence of bromine to give 2-amino 4-substituted phenylthizole and then further treated with chloracetyl chloride to give 2-chloro-N-(4-substituted phenylthizole-2-yl)-acetamide which on refluxing with primary amine gives 15 derivatives. All the synthesized compounds were characterized by IR, H-NMR and elemental analysis.All the synthesized compounds were screened for their antibacterial activity against S. aureus and E. coli by using cup plate agar diffusion method. The activity was measured in terms of zone of inhibition and compared with standard drug ciprofloxacin, sulfonamide. The aminothiazole derivatives were evaluated for antitubercular activity and their result were compared with standard streptomycin.

scholarly journals Antibacterial and Antifungal Activity of Three Monosaccharide Monomyristate Derivatives

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3692 ◽  
Author(s):  
Jumina ◽  
Mutmainah ◽  
Purwono ◽  
Kurniawan ◽  
Syah
Keyword(s):  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Myristic Acid ◽  
Antimicrobial Agents ◽  
Mass Spectral ◽  
Food Industries ◽  
Gram Positive Bacteria ◽  
Microbial Infections ◽  
Antibacterial And Antifungal ◽  
Weak Antibacterial Activity

Microbial infections remains a serious challenge in food industries due to their resistance to some of the well-known antibacterial and antifungal agents. In this work, a novel monomyristoyl ester (fructosyl monomyristate) and two other derivatives (i.e., glucosyl and galactosyl monomyristates) were successfully synthesized from myristic acid and monosaccharides in two-step reactions. First, the myristic acid was converted to myristoyl chloride, and then the myristoyl chloride was reacted with fructose, glucose and galactose separately to produce the corresponding monosaccharide monomyristate derivatives. The structures of the synthesized products were confirmed by Fourier transform infrared (FTIR), proton and carbon nuclear magnetic resonance (1H- and 13C-NMR), and mass spectral (MS) data. The monomyristates esters were obtained in reaction yields of 45.80%–79.49%. The esters were then evaluated for their antimicrobial activity using the disc diffusion test. It was found that the esters exhibited a medium antibacterial activity against gram-positive bacteria; however, they showed a weak antibacterial activity against gram-negative bacteria. Amongst the esters, galactosyl myristate yielded the highest antibacterial activity against Salmonella typhimurium, Staphylococcus aureus and Bacillus subtilis, while glucosyl monomyristate exhibited the highest antibacterial activity only against Escherichia coli. Additionally, all products showed remarkable antifungal activity against Candida albicans. These findings demonstrate that monosaccharide monomyristate derivatives are promising for use as biocompatible antimicrobial agents in the future.

Antioxidant and Antimicrobial Activity of Canavalia gladiata

2021 ◽  
pp. 6507-6510
Author(s):  
Sai Koteswar Sarma ◽  
D. Umamaheswari ◽  
B. R. Balakrishnan
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Standard Drug ◽  
Canavalia Gladiata ◽  
Unstable Molecules ◽  
Antioxidant And Antimicrobial Activity ◽  
Agar Well Diffusion Method

The present study deals with the antioxidant and antimicrobial activities of Canavalia gladiata. Antioxidant activity by nitric oxide and DPPH methods reveals that methanol extract of Canavalia gladiata shows good results when compared to the aqueous extract. It indicates that methanol extract of Canavalia gladiata shows maximum percentage of inhibition when compared to the standard drug (Ascorbic acid).Antioxidants help to neutralize free radicals, which are unstable molecules that are linked to the development of a number of degenerative diseases such as cancer, cardiovascular disesase, cognitive impairment immune dysfunction, cataract and macular degeneration. Antibacterial activity by Agar well diffusion method reveals that methanol extract of Canavalia gladiata shows better results when compared to the aqueous extract. It indicates that methanol extract of Canavalia gladiata shows maximum zone of inhibition when compared to the the standard drug (chloramphenicol).

scholarly journals ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF HEDYOTIS CORYMBOSA, INULA RACEMOSE, HOLOSTEMMA ADAKODIEN MEDICINAL PLANTS

2019 ◽  
pp. 25-29
Author(s):  
AJIT KUMAR MARISETTI ◽  
VENKATARATHANAMMA V.
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
Microbial Activity ◽  
Positive Control ◽  
Diffusion Method ◽  
Antibacterial And Antifungal Activity ◽  
Zone Of Inhibition ◽  
Activity Screening ◽  
Antibacterial And Antifungal ◽  
Anti Fungal

Objective: To evaluate the antibacterial and antifungal activity of three different medicinal plants invitro. Oil extracts of the plants from three different families (Hedyotis corymbosa, Inula racemose, Holostemma adakodien) are used to find the antibacterial and antifungal activity. Methods: Study of anti-microbial activity using agar cup diffusion method, inoculums preparation, Anti-bacterial activity screening (well diffusion method), Anti-fungal activity screening (well diffusion method) are the different methods which are used to find the anti-bacterial and anti-fungal activities of the oil extracts of the above medicinal plants. The activity of these oil extracts and the selected antibiotics are evaluated against four bacterial strains (E. Coli, S. aureus and P. aeruginosa) and three Candida fungal strains (albicans, parapsilosis and tropicalis.). The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin. Results: The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin. Further studies are needed to explore the novel antibacterial bioactive molecules. Conclusion: The plants Hedyotis corymbosa, Inula racemose and Holostemma adakodien have shown significant anti-bacterial and anti-fungal activity.

scholarly journals Antimicrobial Activities of Mint Lemonade Plant Extracts from Salt and Heavy Metal Stressed Plants

2021 ◽  
pp. 112-122
Author(s):  
Asima Khan ◽  
Qurban Ali ◽  
Muhammad Awais Javeid ◽  
Arif Malik
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Bacillus Cereus ◽  
Plant Extracts ◽  
Aeromonas Hydrophila ◽  
Zone Of Inhibition ◽  
Antifungal Activities ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal

Aim of present research was to evaluate the anti-bacterial, anti-fungal, anti-oxidant and multi-stress activities of a medicinal plant Mint Lemonade plant extract. Fresh mint was purchased from the nearest market. The samples were cleaned with distilled led water and its leaves, stem and roots were separated them all. The material was dried in shade at room temperature (24ºC). The three types of extracts were prepared for each sample of mint plantviz, ethanol, n-hexane and water. The antibacterial and antifungal activities of ethanolic, n-haxene and aqueous mint extract was determined on Escherichia coli, Bacillus cereus, Pseudomonas, Aeromonas hydrophila, Aspergillus niger, Aspergillus flavous and Rhizopus stolonifer respectively. From the results of the antibacterial and antifungal activities it was clear that the aqueous extract showed no inhibitory effect on test these microorganisms. In ethanolic extract of antibacterial activity Escherichia coli showed the maximum zone of inhibition with diameters of 3.90 cm at 50 µl dose. Bacilluscereus showed the zone of inhibition diameter 4.9 cm. Pseudomonas showed the maximum zone of inhibition in diameter 3.86mm.Aeromonas hydrophila expressed the maximum zone of inhibition in diameter 3.86mm. The n-hexane extracts antibacterial activity of E. coli, Bacillus cereus, Pseudomonas and Aeromonas hydrophila showed the zone of inhibition 3.86 cm, 4.5 cm, 7.90 mm and 4.71 cm respectively, while the positive control showed inhibition zone about 0.0 cm. The antifungal activity in n-haxene extract showed the maximum result in Aspergillus flavous 18 cm of the area of inhibition. In ethanol extracts antifungal activity showed the maximum result in Aspergillus flavous 4.8 cm of the zone of inhibition. The inhibitory action of mint plant extracts indicated that the plant may be used as potential antibacterial agent.

scholarly journals Synthesis of some novel 1H-indole derivatives with antibacterial activity and antifungal activity

2020 ◽  
Vol 9 (2) ◽  
pp. 961-967 ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Indole Derivatives ◽  
Standard Drug ◽  
Paper Disk ◽  
Standard Medication ◽  
Tube Dilution

The present research works a series of new 1H-Indole derivatives were synthesized. The title compound was obtained by the reaction of indole with chloroacetylchloride in toluene afforded 2-chloro-1-(indoline-1-yl) ethanone which reacts with 1,4 diamine benzene in chloroform afforded 2-((4-aminophenyl)amino)-1-(1H-indol-1-yl)ethan-1-one, on further reaction with various acetophenone in presence of acetic acid in ethanol gave various final derivatives. After synthesis of compounds, the synthesized compounds were characterized by their IR, 1HNMR spectral data and elemental analysis. These derivatives were screened for their antimicrobial activity (Paper-disk-plate technique (disc diffusion method) and Tube-dilution technique (broth microdilution technique), antifungal activity of all synthesized compounds were evaluated against Aspergillus niger and Candida albicans (ATCC 10231) using Fluconazole as the standard drug using the paper plate method and antibacterial activity against both Gram-positive (Bacillus subtilis) and Gram-negative bacteria (Escherichia coli) using Ampicillin as standard medication at a concentration of 50g/ml, 100g/ml. The collected compounds were evaluated for antibacterial activity and antifungal activity. All compounds exhibit significant antimicrobial activity.

scholarly journals Invitro antibacterial activity of Cassia fistula Linn methanolic leaf extracts

2019 ◽  
Vol 8 (2) ◽  
pp. 270
Author(s):  
Bindhu R. Kamath ◽  
Sabeena Kizhedath
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Cassia Fistula ◽  
Leaf Extracts ◽  
E Coli

Background: Cassia fistula Linn is a plant which is widely grown in India and is used for medicinal purposes. The study was carried out with an objective to demonstrate the antimicrobial activity of leaves of Cassia fistula Linn. The aim of the study is to assess antibacterial and antifungal activity of methanolic leaf extract of Cassia fistula Linn against selected clinical isolates.Methods: The antimicrobial activity of methanolic extract of Cassia fistula was evaluated using agar well diffusion method and to zone of inhibition of extract was determined. Clinical isolates of Staphyloccocus aureus, MRSA, Pseudomonas aeruginosa, E. coli and Proteus were screened.Results: The methanolic extracts exhibited antibacterial activity against Staphylococcus aureus. The extract was not active against E. coli, Proteus, MRSA, Pseudomonas aeruginosa. The extract also failed to demonstrate antifungal activity against Candida albicans and Aspergillus niger.Conclusions: The global emergence of multidrug resistant bacterial strains is increasing, limiting the effectiveness of current drugs and treatment failure of infections. A novel approach to the prevention of antibiotic resistance of pathogenic species is the use of new compounds that are not based on existing synthetic antimicrobial agents.

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