scholarly journals Studies on potential of Withania somnifera root extract against diabetic foot infection pathogens

2017 ◽  
Vol 7 (5) ◽  
pp. 11
Author(s):  
Abdul Kapur Mohamed Mydeen ◽  
Ahmed John Syed Basha
Keyword(s):  
Antibacterial Activity ◽  
Diabetic Foot ◽  
Chemical Constituents ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
Active Fraction ◽  
Antibacterial Study ◽  
Diabetic Foot Infections ◽  
High Degree

Withaniasomnifera phytochemical analysis of root extract showed the presence of alkaloids, flavonoids, tannins saponins, carbohydrates, quinines and phenol compounds. Studies on the prevalence of diabetic foot infections showed a total of 88 isolates belongs to seven different genera. The standard antibacterial study reveals that E.coli and S.aureus were only isolated pathogens showed the high degree of resistant pattern against all tested antibiotics.  S.aureus showed 100 percent resistant to penicillin, ampicillin and amoxicillin. Out of 40 S.aureus isolates, 23 were found to be methicillin resistant and 12 were vancomycin resistant. Similarly out of 22 E.coli, 18 were resistant to penicillin; ampicillin and nine isolates were resistant to amoxicillin. The antibacterial activity of W.somnifera root extract showed potent antibacterial activity at 5mg/mL against E.coli and S.aureus. Bio assay analysis of extracted compounds reveals that the Rf value of the active fraction is 0.38. Gas Chromatography Mass Spectrum of active fraction shows the presence of 7 different chemical constituents concludes further purification of active compound is necessary. The haemolytic study confirms that the extract is safe to use since there is no haemolysis of human RBCs. Further analysis of purification and characterization of an active fraction is required for structural elucidation.

scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF SWIETENIA MAHAGONI – A HIGHLY MEDICINAL PLANT IN INDIA

2016 ◽  
Vol 9 (6) ◽  
pp. 62
Author(s):  
Dhanapaul Vinoth Kumar
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Leaf Extract ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Volatile Oils ◽  
Medicinal Uses ◽  
Antibacterial Study ◽  
Active Constituents ◽  
Swietenia Mahagoni

The present study was designed to evaluate the phytochemical analysis and antibacterial activity of Swietenia mahagoni leaf extract. The leaf extract was subjected to a variety of phytochemical analysis. The qualitative phytochemical tests exhibited the presence of common phytocompounds like alkaloids, tannins, saponins, phlobatannins, flavanoids, terpenoids and volatile oils as major active constituents. The plant (Swietenia mahagoni) leaf has valuable medicinal uses, hence petroleum ether (18.2g) was found to be a good solvent for the phytochemical and antibacterial study. Similarly, the Methanolic leaf extract of Swietenia mahagoni has highest zone of inhibition on Bacillus subtilis.

scholarly journals Microbial profile, antimicrobial resistance, and molecular characterization of diabetic foot infections in a university hospital

GERMS ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 39-51
Author(s):  
Azza A Ismail ◽  
Marwa A Meheissen ◽  
Talaat A Abd Elaaty ◽  
Nermine E Abd-Allatif ◽  
Heba S Kassab
Keyword(s):  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Diabetic Foot ◽  
University Hospital ◽  
Microbial Profile ◽  
Diabetic Foot Infections

Characterization of bacterial isolates from diabetic foot infections in Ile-Ife, Southwestern Nigeria

The Foot ◽  
2006 ◽  
Vol 16 (3) ◽  
pp. 158-164 ◽  
Author(s):  
A.K. Ako-Nai ◽  
I.C. Ikem ◽  
O.O. Akinloye ◽  
A.O. Aboderin ◽  
R.T. Ikem ◽  
...  
Keyword(s):  
Diabetic Foot ◽  
Bacterial Isolates ◽  
Southwestern Nigeria ◽  
Diabetic Foot Infections

Antibiotic Tissue Penetration in Diabetic Foot Infections

2015 ◽  
Vol 105 (6) ◽  
pp. 520-531 ◽  
Author(s):  
Amanda Ray ◽  
Danielle Malin ◽  
David P. Nicolau ◽  
Dora E. Wiskirchen
Keyword(s):  
Diabetic Foot ◽  
Antimicrobial Agents ◽  
Arterial Disease ◽  
Time Profile ◽  
Tissue Homogenate ◽  
Tissue Penetration ◽  
Diabetic Foot Infections ◽  
Pharmacodynamic Profile

Although many antimicrobial agents display good in vitro activity against the pathogens frequently implicated in diabetic foot infections, effective treatment can be complicated by reduced tissue penetration in this population secondary to peripheral arterial disease and emerging antimicrobial resistance, which can result in clinical failure. Improved characterization of antibiotic tissue pharmacokinetics and penetration ratios in diabetic foot infections is needed. Microdialysis offers advantages over the skin blister and tissue homogenate studies historically used to define antibiotic penetration in skin and soft-tissue infections by defining antibiotic penetration into the interstitial fluid over the entire concentration versus time profile. However, only a select number of agents currently recommended for treating diabetic foot infections have been evaluated using these methods, which are described herein. Better characterization of the tissue penetration of antibiotic agents is needed for the development of methods for maximizing the pharmacodynamic profile of these agents to ultimately improve treatment outcomes for patients with diabetic foot infections.

Antibacterial Activity of Halophilic Bacteria Against Drug-Resistant Microbes Associated with Diabetic Foot Infections

2020 ◽  
Vol 77 (11) ◽  
pp. 3711-3723
Author(s):  
Santhaseelan Henciya ◽  
Thasu Dinakaran Vengateshwaran ◽  
Murugaiah Santhosh Gokul ◽  
Hans-Uwe Dahms ◽  
Rathinam Arthur James
Keyword(s):  
Antibacterial Activity ◽  
Diabetic Foot ◽  
Halophilic Bacteria ◽  
Drug Resistant ◽  
Diabetic Foot Infections

scholarly journals Green Synthesis and Characterization of Silver Nanoparticles using Crude Extract of Crotalaria burhia

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Khalil Ahmad ◽  
Raeesa Noor ◽  
Muhammad Younus ◽  
Akram Chohan ◽  
Ume Habiba ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Zeta Potential ◽  
Green Synthesis ◽  
Crude Extract ◽  
Bacterial Infections ◽  
Synthesis And Characterization ◽  
Phytochemical Analysis ◽  
E Coli

Background: Appearance of antibiotic resistance has raised the demand to find alternative therapies and modified drug delivery system of medicinal plants to treat bacterial infections. Objective: The aim of this study is the green synthesis and characterization of silver nanoparticles by using crude extract of Crotalaria burhia and to evaluate their antibacterial potential. Methods: The roots and stems of plant were used to prepare the crude extract. The phytochemical analysis of different compounds in extract was performed. 1mM AgNO3 and different concentrations of plant extract were used for the green synthesis of silver nanoparticles. The particles size and zeta potential were measured by zeta sizer while surface morphology of silver nanoparticles was observed with Scanning Electron Microscope (SEM). The antibacterial activity of silver nanoparticles was performed by 96 well microdilution plate method. Results: The particle size and zeta potential of optimized formulation was 92 nm and -24.8 mV. The SEM analysis showed that silver nanoparticles are irregular and spherical shape. The antibacterial activity showed that MIC value of silver nanoparticles was lower for E. coli than S. aureus. Conclusion: Silver nanoparticles possess potent bactericidal activity against E. coli and moderate activity against S. aureus. It had been concluded that these nanoparticles can be used against multi-drug resistant bacterial infections.

Antibacterial Activity of Anthocliesta vogelii (planch) and Tinospora cordifolia/ on Esherichia coli and Pseudomonas aeruginosa

2020 ◽  
Vol 15 (3) ◽  
pp. 50-55
Author(s):  
S.B Adeyemi ◽  
A. I Afonja ◽  
M.B Odebisi-Omokanye ◽  
T.P Okor ◽  
A.A Lateef
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Stem Bark ◽  
Tinospora Cordifolia ◽  
Phytochemical Analysis ◽  
Root Extract ◽  
E Coli ◽  
Esherichia Coli ◽  
Test Organisms

An ethnobotanical investigation has revealed that the roots of Anthocliesta vogelii and stem bark of Tinospora cordifolia are used by indigenous people in the treatment of pile. This present study evaluated the antibacterial activity of the root and stem bark of Anthocliesta vogelii and Tinospora cordifolia respectively against the human pathogenic gram-negative bacteria Pseudomonas aeruginosa and Escherichia coli. The phytochemical composition of these two plants was also assayed. Graded concentrations of 25mg/ml, 50mg/ml, 100mg/ml and 200mg/ml of the respective crude extract were applied against the test organisms. The percentage yield of the ethanolic extract of T. cordifolia stem and A. vogelii root were 40.978% and 21.872% respectively with their phytochemical analysis revealing the presence of saponins, alkaloids, tannins, flavonoids, and glycosides, with steroids present in A. vogelii but absent in T. cordifolia. The antimicrobial effect of the ethanolic extracts of both plants on the two test bacteria was significant (P<0.05). However, the ethanolic root extract of A. vogelii showed higher inhibition against P. aeruginosa and E. coli than the ethanolic stem extract of T. cordifolia at concentrations of 50mg/ml, 100mg/ml and 200mg/ml, while both extracts did not have any inhibition on the test organisms at a concentration of 25mg/ml. The Minimum Inhibitory Concentrations (MICs) of the ethanolic extract of A. vogelii root on P. aeruginosa and E. coli were 50mg/ml and 100mg/ml respectively, while the MICs for the ethanolic extract of T. cordifolia stem on P. aeruginosa and E. coli was 50mg/ml respectively. Both plant extracts could not kill the test bacteria; hence, they are only bacteriostatic and not bactericidal against the test bacteria. Keywords: Anthocleista vogelii, Tinospora cordifolia, Bacterial, Bacteriostatic, Bactericidal

scholarly journals Histochemical and Phytochemical Analysis of Medicinally Important Plants

2020 ◽  
pp. 1-13
Author(s):  
B. K. Sushma ◽  
K. S. Ashalatha ◽  
Preetam Ray ◽  
H. R. Raveesha
Keyword(s):  
Phytochemical Analysis ◽  
Root Extract ◽  
Vascular Bundles ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Isolation And Characterization ◽  
Phytochemical Studies ◽  
Maximum Content

The present study was aimed to investigate the histochemical and phytochemical studies of four important medicinal plants. Leaf, stem and root sections were examined for alkaloid, protein and phenolic tests. Methanolic extracts of leaf, stem and root were analysed for the phytochemical screening of major bioactive constituents. Histochemical analysis reveals the presence of alkaloids, phenols and proteins in epidermis, cortex and vascular bundles of root, stem and leaf. Preliminary phytochemical analysis showed the presence of alkaloids, tannins, phenols, flavonoids, steroids, saponins, terpenoids, coumarins, quinone and proteins. The root extract of Catharanthus roseus recorded maximum content of phenols compared to other extracts. Costus pictus root and leaf extracts exhibited a significant amount of flavonoids and tannins respectively. Further studies were focused on isolation and characterization of each plant compounds.

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