scholarly journals Analysis of peripheral blood white blood cell parameters in White stork (Ciconia ciconia L.) chicks varies by sex

2018 ◽  
Author(s):  
Monika Grandtke ◽  
Mariusz Kasprzak ◽  
Mateusz Ciepliński ◽  
Ewa Burda ◽  
Ariel Durajski ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Blood Film ◽  
White Stork ◽  
Qualitative And Quantitative Analysis ◽  
Cell Parameters ◽  
White Storks ◽  
Males And Females ◽  
Dark Blue

The aim our study was qualitative and quantitative analysis of white blood morphometric elements of peripheral blood (determining the quantity, blood cells dimension and several hematological values) in White Stork chicks. One of the aims was to indicate whether the sex relevantly influences the variety of the examined white blood indicators. White blood cells parameters of 53 White Stork chicks, with molecularly marked sex, was examined. Blood samples were collected in southern Poland (around Krapkowice town, near Opole city). Lymphocytes of White Storks (mean 37% for females and males) were identified as round cells with dark purple non-lobed, eccentrically positioned nucleus. Among the whole population we differentiated small lymphocytes: with diameters of 5.31 ± 0.65 μm in males and 5.57 ± 0.59 μm in females, and large lymphocytes with a diameter of 8.10 ± 0.66 μm and 8.28 ± 0.74 μm, respectively in females and males. Monocytes (mean 8% for female and males) were the largest leukocytes found in the blood film of White Stork, measuring 13.40 ± 0.97 μm for males and 13.09 ± 1.05 μm for females in diameter. The cytoplasm was abundant and it stained blue-gray, very often contained vacuoles. Heterophils (mean 42.7%) were the largest in granular leukocytes group. They were round and 11.14 ± 0.65 μm for males and 11.01 ± 0.48 μm for females in diameter. The nucleus of heterophils was lobed, usually with two or three lobes. The cytoplasm contained brick-red, elongated granules. Eosinophils (mean 9.44%) were round cells, with a diameter of 10.72 ± 0.49 μm and 10.97 ± 0.53 μm, respectively in males and females. The nucleus was lobed and mostly stained clear blue and contained red-orange, round or rod-shaped granules. Basophils (mean 1.84%) were round and contained dark blue granules, with average of 9.56 ± 0.78 and 9.13 ± 0.84 μm in diameter, for males and females. The nucleus was usually non-lobed. The H/L ratio was 4/4 for both sexes. No significant differences in levels and types of leukocytes between male and female juvenile storks have been observed.

scholarly journals Adult Mice With Targeted Mutation of the Interleukin-11 Receptor (IL11Ra) Display Normal Hematopoiesis

Blood ◽  
1997 ◽  
Vol 90 (6) ◽  
pp. 2148-2159 ◽  
Author(s):  
Harshal H. Nandurkar ◽  
Lorraine Robb ◽  
David Tarlinton ◽  
Louise Barnett ◽  
Frank Köntgen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Bone Marrow Cells ◽  
White Blood Cells ◽  
Null Mutation ◽  
Wild Type ◽  
Normal Numbers ◽  
Interleukin 11 ◽  
Marrow Cells

Abstract Interleukin-11 (IL-11) is a pleiotropic growth factor with a prominent effect on megakaryopoiesis and thrombopoiesis. The receptor for IL-11 is a heterodimer of the signal transduction unit gp130 and a specific receptor component, the α-chain (IL-11Rα). Two genes potentially encode the IL-11Rα: the IL11Ra and IL11Ra2 genes. The IL11Ra gene is widely expressed in hematopoietic and other organs, whereas the IL11Ra2 gene is restricted to only some strains of mice and its expression is confined to testis, lymph node, and thymus. To investigate the essential actions mediated by the IL-11Rα, we have generated mice with a null mutation of IL11Ra (IL11Ra−/−) by gene targeting. Analysis of IL11Ra expression by Northern blot and reverse transcriptase-polymerase chain reaction, as well as the absence of response of IL11Ra−/− bone marrow cells to IL-11 in hematopoietic assays, further confirmed the null mutation. Compensatory expression of the IL11Ra2 in bone marrow cells was not detected. IL11Ra−/− mice were healthy with normal numbers of peripheral blood white blood cells, hematocrit, and platelets. Bone marrow and spleen contained normal numbers of cells of all hematopoietic lineages, including megakaryocytes. Clonal cultures did not identify any perturbation of granulocyte-macrophage (GM), erythroid, or megakaryocyte progenitors. The number of day-12 colony-forming unit-spleen progenitors were similar in wild-type and IL11Ra−/− mice. The kinetics of recovery of peripheral blood white blood cells, platelets, and bone marrow GM progenitors after treatment with 5-flurouracil were the same in IL11Ra−/− and wild-type mice. Acute hemolytic stress was induced by phenylhydrazine and resulted in a 50% decrease in hematocrit. The recovery of hematocrit was comparable in IL11Ra−/− and wild-type mice. These observations indicate that IL-11 receptor signalling is dispensable for adult hematopoiesis.

scholarly journals Bone Marrow and Peripheral Blood Cells Toxicity of a Single 2.0 Gy Cobalt60 Ionizing Radiation: An Animal Model

1970 ◽  
Vol 29 (2) ◽  
Author(s):  
Shittu Akeem ◽  
Olatunbosun Lukman ◽  
Khalil Eltahir ◽  
Olalere Fatai ◽  
Babatunde Abiola ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Marrow Cell ◽  
P Value ◽  
Peripheral Blood Cells ◽  
P Values ◽  
Cell Parameters ◽  
Post Irradiation ◽  
Total Body

BACKGROUND: Bone marrow is extremely vulnerable to damage caused by radiation therapy. Hence, bone marrow suppression is an important side effect of radiotherapy. Effective use of radiotherapy is therefore compromised by radiation-related injuries.MATERIAL AND METHODS: Six Guinea-pigs were recruited for the study of which three were subjected to total body irradiation with Co60 while the other three served as controls. Bone marrow and peripheral blood samples were collected before and at days 9, 14 and 21, post irradiation. Manual and automated counts were performed for bone marrow nucleated cells and peripheral blood cells respectively.RESULTS: Declining bone marrow cellularity was evident immediately post irradiation. Mean ± SD of marrow cell counted per mm3 were 121,924±281, 87,603±772, 121,367±375 and122,750±1000 pre-irradiation and days 9, 14 and 21, postirradiation (p-values 0.10, 0.27 and 0.29 respectively). Significant drops in counts were noticed on day 9 post-irradiation for all red cell parameters (p-values <0.05), for Total White Blood Cell Count and Neutrophil count (p-values <0.05) and also on days 14 and 21 for Lymphocytes (p-values <0.05) and on day 21 for Eosinophil/Basophil/Monocytes (p-value <0.05). A significant drop in platelets counts was also noticed on day 9 (p-value <0.05) which significantly increased above pre-irradiation value on day 21.CONCLUSION: Total body irrradiation with Co60 significantly affects the bone marrow with maximum reductions in marrow nucleated cells and peripheral blood cells counts on day 9 post irradiation. 

scholarly journals The prevalence of leukocyte abnormalities among Sudanese patients with sickle cell disease

2021 ◽  
Vol 9 (1) ◽  
pp. 262-267
Author(s):  
Tarig Osman Khalafallah Ahmed ◽  
Ekhlas Alrasheid Abu Elfadul ◽  
Ahmed A. Agab Eldour ◽  
Omer Ibrahim Abdallah Mohammed
Keyword(s):  
Sickle Cell Disease ◽  
Cell Volume ◽  
Sickle Cell ◽  
Blood Cells ◽  
White Blood Cells ◽  
Blood Film ◽  
Cell Disease ◽  
Mean Cell Volume ◽  
Blood Disorder ◽  
Age Range

Sickle cell disease (SCD) is an inherited blood disorder that affects red blood cells. The study was conducted in Elobied town during the period May 2011 to September 2011. The aim of this study is to detect the abnormalities of leucocytes among sickle cell anemic patients. 40 sickle cell anemic patients; age range between 8 months to 23 years. Blood sample was taken for all patients and the laboratory investigation were performed using automated estimation for: hemoglobin (Hb), Packed cell volume (PCV), red cell count (RBCs), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell concentration (MCHC), and total white blood cells, comment on blood film using manual methods. The conclusion of this study there is increase in total white blood cells with shift to left in neutrophil precursor in sickle cell patients with complications ,the most immature cells are band form, myelocytes and metamyelocytes, and there also lymphocytosis and neutrophilia which has been increases in response to infections.

Development of a robust algorithm for detection of nuclei of white blood cells in peripheral blood smear images

2019 ◽  
Vol 78 (13) ◽  
pp. 17879-17898 ◽  
Author(s):  
Roopa B. Hegde ◽  
Keerthana Prasad ◽  
Harishchandra Hebbar ◽  
Brij Mohan Kumar Singh
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
White Blood Cells ◽  
Peripheral Blood Smear ◽  
Robust Algorithm

ROBUST DISCRIMINATION OF LEUKOCYTES PROTUBERANT TYPES FOR EARLY DIAGNOSIS OF LEUKEMIA

2019 ◽  
Vol 19 (06) ◽  
pp. 1950055 ◽  
Author(s):  
ISRA NAZ ◽  
NAZEER MUHAMMAD ◽  
MUSSARAT YASMIN ◽  
MUHAMMAD SHARIF ◽  
JAMAL HUSSAIN SHAH ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Blood Cells ◽  
Detection System ◽  
White Blood Cells ◽  
Training Model ◽  
Blood Film ◽  
Morphological Examination ◽  
Efficient Detection ◽  
Laboratory Technology

Advanced laboratory technology has made blood testing more automated, robust and tests are being implemented comprehensively. Leukocytes type differentiation is a critical hematological images analysis step of blood film as it delivers valuable information in diagnosis of several diseases. At present, the morphological examination of leukocytes is done manually and this process is very tedious, inefficient and slow. Although many white blood cells detection or classification techniques are presented by different researchers, there is still a need of fully automated and an efficient detection system of blood cells with its particular types for an early diagnosis of leukemia. This paper presents a technique for the classification of protuberant types of leukocytes and early diagnosis of leukemia. The work is divided into the following main stages: (a) image augmentation, (b) wavelet composition and decomposition for attaining high and low frequency bands of the cell image, (c) convolutional neural network (CNN) training model for the classification of leukocytes categories and (d) prediction of leukemia. The main intention behind this study is to develop an automated, robust and efficient classification and detection system of leukocytes for microscopic blood images.

scholarly journals Circulating stem cells in mice treated with cyclophosphamide

Blood ◽  
1992 ◽  
Vol 80 (1) ◽  
pp. 264-269 ◽  
Author(s):  
CF Craddock ◽  
JF Apperley ◽  
EG Wright ◽  
LE Healy ◽  
CA Bennett ◽  
...  
Keyword(s):  
Stem Cells ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Cultured Cells ◽  
Autologous Transplantation ◽  
White Blood Cells ◽  
X Rays ◽  
Donor Cells ◽  
Macrophage Colony

Abstract Chemotherapy has been used clinically to mobilize hematopoietic progenitor cells into the peripheral blood so that they can be harvested for autologous transplantation. In humans, this is demonstrated by the presence of circulating granulocyte-macrophage colony-forming cells (CFU-GM) and CD34-positive cells, but it has not been possible to confirm the presence of marrow-repopulating stem cells. In this study, we treated mice with 200 mg/kg cyclophosphamide (CY) and measured the numbers of white blood cells, day 12 CFU-S (CFU- S12), and CFU-GM in the peripheral blood. There was a peak in the numbers of CFU-S12 and CFU-GM 8 days after treatment with cyclophosphamide. Peripheral blood cells taken at this time rescued lethally irradiated mice and engraftment of donor cells was confirmed after 140 days in sex mismatched recipients using a Y chromosome- specific probe. In vitro culture of the blood cells harvested after cyclophosphamide showed that they proliferated in suspension cultures for at least a year in the presence of interleukin-3. The cultured cells rapidly lost their abilities to rescue irradiated mice and to form colonies in vitro, but they did not become leukemic. Also, CY- treated mice were irradiated with a leukemogenic dose of x-rays to coincide with peak circulating cell numbers but these animals did not develop an excess of leukemias over mice given irradiation alone.

scholarly journals Megaloblastosis Produced by a Cytosine Antagonist

Blood ◽  
1963 ◽  
Vol 21 (3) ◽  
pp. 352-362 ◽  
Author(s):  
R. W. TALLEY ◽  
V. K. VAITKEVICIUS
Keyword(s):  
Bone Marrow ◽  
Dna Synthesis ◽  
Mechanism Of Action ◽  
Cytosine Arabinoside ◽  
Peripheral Blood ◽  
Blood Cells ◽  
White Blood Cells ◽  
Structural Similarity ◽  
Mitotic Abnormalities ◽  
Temporary Decrease

Abstract 1. Cytosine arabinoside induced objective, but temporary, decrease of tumor masses in three patients with lymphosarcoma and slight decrease in some lesions in two out of ten treated patients with disseminated carcinomatosis. 2. In doses of 3 to 50 mg./Kg. given at varying intervals, cytosine arabinoside induced definite megaloblastic changes in the marrow of all patients studied. Mitotic abnormalities similar to those found in other megaloblastic anemias also occurred. 3. Associated with bone marrow changes, depressions of hemoglobin, white blood cells and platelets in the peripheral blood were observed. 4. The exact mechanism of action of cytosine arabinoside has not been elucidated. It is speculated that because of the close structural similarity between cytidylic acid, cytosine arabinoside could interfere with DNA synthesis.

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