scholarly journals Different Aspergillus niger Strains and Inoculum Levels Affect the Nutritional Composition of Olive Leaves in Solid-state Fermentation

Author(s):  
Aydın Altop ◽  
Emrah Güngör ◽  
Güray Erener

This study was conducted to investigate the effects of Aspergillus niger strain and inoculum level on the nutritional composition of olive leaves. The experiment had a 2×3 factorial arrangement of treatments with two A. niger strains (ATCC 200345 [A] and ATCC 9142 [B]) and three inoculum levels (104, 106, and 108). Olive leaves were milled to 2 mm and fermented in solid-state by two different A. niger strains and analysed for nutritional composition. Crude protein (CP) was increased linearly as the inoculum level increased. Ether extract (EE) and crude fiber (CF) were decreased linearly in A strain or quadratically in B strain with increased inoculum levels. Crude ash and nitrogen-free extract content did not differ among groups. Strain A increased neutral detergent fiber (NDF) at higher inoculum level, whereas NDF was decreased as inoculum level increased in B strain. The lowest acid detergent fiber (ADF) was obtained from the B strain or 106 inoculum level. The results showed that the effect of two strains on CP and ADF changed similarly with increased inoculum levels. However, two strains affected EE, CF, and NDF content differently with increased inoculum levels. The optimal situations were 108 inoculum level for higher CP, A104 or B104 for higher EE, B106 for lower CF, B106 or B108 for lower NDF, 106 inoculum level or B strain for lower ADF. B strain at 106 inoculation level can be preferred to obtain an average CP and EE content and lower CF, NDF, and ADF content.

Author(s):  
Emrah Güngör ◽  
Aydın Altop ◽  
Guray Erener

The study was aimed to improve the nutritional composition of nettle (Utrica dioica L.) having positive effects on animals by solid-state fermentation. Aspergillus niger was used as a inoculant in fermentation of nettle. Before and after fermentation, crude protein (CP), ash, ether extract (EE), nitrogen-free extract (NFE), crude fiber (CF), hemicellulose (HC), neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) contents of nettle were determined. The ash, NFE and HC content of nettle were increased by fermentation. However, A. niger decreased the CF and EE in nettle. The results showed that solid-state fermentation with A. niger can be used to improve the nutritional composition of nettle.


Author(s):  
Aydın Altop

This study aimed to investigate the effect of solid-state fermentation on the nutritional composition of Agaricus bisporus mushroom stalk and cap. Four homogenous test samples were prepared from the fermented mushroom stalk (Fermented MS), unfermented mushroom stalk (MS), fermented mushroom cap (Fermented MC), and unfermented mushroom cap (MC); each was independently analyzed in triplicates, providing a total of 12 independent measurements of all treatments. Nutritional changes in the mushrooms were determined by analyzing crude protein (CP), ether extract (EE), crude ash (CA), crude fiber (CF), hemicellulose, neutral detergent fiber (NDF), acid detergent lignin (ADL), and acid detergent fiber (ADF) contents. The best improvement in nutritional composition was obtained from the MS with increasing the crude protein (35 %). The results related to other parameters are similar among all the treatments, except for CF. To conclude, the fermented MS can be considered as an alternative protein resource in animal nutrition.


Author(s):  
F. Almeida López ◽  
P. Toalombo Vargas ◽  
S. Jiménez Yánez ◽  
M. Chávez Haro

This study aimed to analyze the nutritional characteristics of Medicago sativa (Alfalfa) hay for the feeding of ruminants in the central zone of the Sierra Region. The study was conducted in the Laboratory of the Faculty of Pecaurias Sciences of the Higher Polytechnic School of Chimborazo. Cutting was carried out for 60 days in different areas of the central Sierra region. Data were analyzed using descriptive statistics. Results of the bromatological analysis showed that the average total humidity was 11.12 ± 0.20% in (Rp-00542) while the minimum was 7.79 ± 0.05% in (Rp-00224). Values for dry matter ranged between 91.25 ± 0.03% (Rp336) and 88.53 ± 0.09% (Rp492); dry base ash ranged between 12.51 ± 0.08% (Rp500) and 9.16 ± 0.08% (Rp521); dry organic matter ranged between 90.84 ± 0.13% (Rp521) and 87.49 ± 0.08% (Rp521); and dry crude protein ranged between 22.44 ± 0.26% (Rp492) and 18.73 ± 0.08% (Rp336). The following values were also obtained: 1.94 ± 0.93% dry ethereal extract; 32.06 ± 2.11% dry nitrogen free extract; 46.96 ± 3.78% dry neutral detergent fiber; 40.67 ± 3.36% dry acid detergent fiber; and 8.23 ± 0.64% dry lignin detergent acid. Therefore, it can be determined that forage mixture provides all of the nutrients to the species of zootechnical interest. Keywords: Medicago sativa, alfalfa, sierra centro, rumiants. RESUMEN En el Laboratorio de la Facultad de Ciencias Pecaurias de la Escuela Superior Politécnica de Chimborazo, se realizó la Caracterización Nutricional de henos de Medicago sativa (Alfalfa) para la alimentación de Rumiantes en la zona centro de la Región Sierra; Mediante estadística descriptiva se obtuvo los siguientes resultados del análisis bromatológico a los 60 días de corte en las diferentes zonas de la región Sierra - Centro, se puede observar que el mayor promedio de la humedad total fue 11,12 ± 0,20% en (Rp-00542) mientras que el menos fue 7,79 ± 0,05% en (Rp-00224); para Materia Seca-Total los valores se obtuvieron entre  91,25 ± 0,03% (Rp336) y 88,53 ± 0,09% (Rp492); Cenizas base seca 12,51 ± 0,08% (Rp500) y 9,16 ± 0,08% (Rp521); el mayor valor para % Materia Orgánica Base seca fue de 90,84 ± 0,13% (Rp521) y el menor con 87,49 ± 0,08% (Rp521); para % Proteína cruda base seca la mejor respuesta fue 22,44 ± 0,26 (Rp492) % y el menor  18,73 ± 0,08 (Rp336), Fibra cruda base seca; 1,94 ± 0,93% Extracto etéreo base seca; 32,06 ± 2,11% Extracto Libre de Nitrógeno base seca; 46,96 ± 3,78% Fibra Detergente Neutro base seca; 40,67 ± 3,36% Fibra Detergente Acida base seca; 8,23 ± 0,64% Lignina Detergente Acida base seca. Por lo que se puede determinar que la mezcla forrajera aporta todos los nutrientes a todas las especies de interés zootécnico. Palabras clave: Medicago sativa, alfalfa, sierra centro, rumiantes.


2019 ◽  
Vol 40 (5Supl1) ◽  
pp. 2397
Author(s):  
Clésio dos Santos Costa ◽  
Rosane Cláudia Rodrigues ◽  
Ricardo Alves de Araújo ◽  
Francisco Naysson de Sousa Santos ◽  
Giovanne Oliveira Costa Sousa ◽  
...  

he aim of this study was to evaluate fermentation and chemical characteristics and the in situ degradability of sorghum silages enriched with dried Leucaena. The experiment was conducted as completely randomized design with four treatments (0 - control silage, silage without leucaena; 10.0, 20.0, or 30.0% inclusion of Leucaena) and five replicates, totaling 20 experimental units. The inclusion levels of leucaena influenced (P 0.05), with all silages having values above 90%. Gas loss decreased (P < 0.05) as the level of leucine increased in sorghum silage. A linear increase was observed for dry matter (P ? 0.0001) and crude protein (P = 0.0008) contents in response to Leucaena inclusion. There was a linear (P < 0.05) linear effect on neutral detergent fiber, hemicellulose and acid detergent fiber. As leucaena inclusion levels increased, for each 1% inclusion a reduction of 0.179 was observed; 0.059 and 0.119% for the values of neutral detergent fiber, hemicellulose and acid detergent fiber respectively. The inclusion of leucaena influenced (P < 0.05) the increase of silage lignin levels, probably due to the higher lignin content of the legume. The soluble fraction (a) of the silages increased along with the Leucaena inclusion levels, with highest values observed at the inclusion of 20 and 30% of the legume, respectively. Potential degradation (A) increased linearly with the levels of Leucaena added to the silage; the highest value for this variable was observed at 30% inclusion. Effective degradability (ED) increased up to the inclusion level of 20% (46.77%). Degradation rate (c) decreased markedly with the use of 30% Leucaena. The use of the forage part of Leucaena dried for three hours leads to reduced gas production, increased dry matter and crude protein contents, and decreased fiber contents. The degradability of dry matter increases up to the inclusion level of 20%.


2017 ◽  
Vol 13 (1) ◽  
pp. 23 ◽  
Author(s):  
Crhisterra E. Kusumaningrum ◽  
Arthalia Poetri Yunisa ◽  
Nana Mulyana ◽  
Suharyono Suharyono

Penelitian dilakukan untuk mengetahui pengaruh pemberian Aspergillus niger iradiasi sinar gamma dosis rendah pada jerami padi fermentasi dan potensinya sebagai pakan ternak ruminansia secara in vitro. Proses fermentasi dilakukan dengan teknik Solid State Fermentation (SSF) menggunakan A. niger yang diiradiasi sinar gamma dosis rendah untuk meningkatkan kemampuan memecah ikatan selulosa. Orientasi dosis iradiasi sinar gamma sebesar 0, 125, 250, 375, 500, 625 dan 750 Gy dilakukan pada A. niger untuk mengetahui kondisi yang optimum dalam menghasilkan aktivitas enzim selulase. A. niger iradiasi sinar gamma pada dosis yang optimum digunakan untuk pembuatan jerami padi fermentasi dan dilakukan evaluasi kemampuan jerami padi fermentasi sebagai pakan ternak ruminansia secara in vitro. Perlakuan jerami padi fermentasi yaitu P0. Jerami Padi, P1. Jerami padi fermentasi A. niger 0 Gy dan P2. Jerami padi fermentasi A. niger 500 Gy. Hasil menunjukkan bahwa dosis optimum iradiasi sinar gamma pada A. niger dalam menghasilkan enzim selulase (3,24 U/ml) adalah 500 Gy. Pada jerami padi fermentasi A. niger 500 Gy menunjukkan aktivitas enzim selulase tertinggi yaitu sebesar 48,5384 U/g bahan kering (BK) dan kadar glukosa sebesar 247,33 mg/g BK pada hari ke-14. Proses fermentasi dengan A. niger iradiasi sinar gamma (500 Gy) mampu meningkatkan kandungan proein kasar, acid detergent fiber (ADF), neutral detergent fiber (NDF) dan bahan kering, namun menurunkan kandungan lemak kasar dan bahan organik (BO). Hasil fermentasi dalam cairan rumen dari jerami padi fermentasi dengan dengan A. niger iradiasi sinar gamma (500 Gy) menunjukkan nilai pH sebesar 7,01; konsentrasi ammonia dan volatile fatty acid (VFA) masing-masing sebesar 3,92% dan 4,73 mmol/100.


2019 ◽  
Vol 33 (3) ◽  
pp. 182-194
Author(s):  
Wagner Sousa-Alves ◽  
João-Paulo S Rigueira ◽  
Marielly M Almeida-Moura ◽  
Diego L S De-Jesus ◽  
Flávio P Monção ◽  
...  

Background: Dry matter (DM) loss is high in sugarcane silage due to its high content of soluble carbohydrates which favors yeast growth and ethanol production, thus reducing the nutritional value of the preserved forage. Objective: To determine the effect of adding two types of urea at two concentration levels to sugarcane silage on the nutritional value and fermentative characteristics of the silage. Methods: A completely randomized 2×2+1 factorial design was used, based on two types of urea (conventional and protected), two urea concentration (0.5 and 1.0% of the natural matter), and a control (sugarcane silage with no additives). Six replicates were used. The mixture was placed in PVC silos and opened after 60 days. Results: There was no difference in DM content or effluent losses by the addition of urea. In regards to neutral detergent fiber, acid detergent fiber, and lignin, decreased levels were observed compared to the control, regardless of urea type. Urea type and concentration had an effect on ammoniacal nitrogen content, with higher values for conventional urea, but not affecting the pH. The addition of urea to sugarcane silage reduced gas losses and raised the levels of crude protein, ether extract, and effective degradability. Conclusion: Adding protected or conventional urea at 1% of natural matter at the time of sugarcane ensiling reduces gas losses and improves nutritional composition of the silage.


Author(s):  
Aydın Altop ◽  
Emrah Güngör ◽  
Güray Erener

This study aimed to investigate the effect of solid-state fermentation on the nutritional composition of cottonseed meal (CSM), sunflower meal (SFM) and hazelnut kernel meal (HKM). In this study, each feedstuff was divided into two treatment groups (unfermented and fermented) with five replicates. Nutritional changes in the feedstuffs were determined by analyzing crude protein, ether extract, ash, crude fiber, neutral detergent fiber, acid detergent fiber contents. Solid-state fermentation had effects on the nutritional composition in all feedstuffs. The best improvement in nutritional quality was obtained from the HKM with increasing the crude protein and decreasing the structural carbohydrates content. Fermented HKM can be considered as an alternative protein feed for soybean meal considering the nutritional composition.


2019 ◽  
Vol 41 (1) ◽  
pp. 45708
Author(s):  
Fabiane Hoffmann ◽  
Maria Giovana Binder Pagnoncelli ◽  
Lilian Regina Rothe Mayer ◽  
Ana Carolina Fluck ◽  
Rodrigo Macagnan ◽  
...  

Sugar cane is highly productive (dry matter.hectare-1), but after ensiling process nutritional quality is affected, thus additives are needed to control or minimize losses. This study aimed to evaluate if Lactobacillus plantarum LPBR01 strain used as silage inoculant for sugar cane can control fermentation losses. Sugar cane samples (72) were divided in two treatments with three replicates, control (no Lactobacillus) and treatment silage with Lactobacillus (106 CFU g-1 of silage). Nutritional composition of samples in different periods of fermentation (0, 7, 15, 30 and 45 days) was estimated by determining levels of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM), mineral matter (MM) and acid detergent lignin (ADL). Fermentative profile of the silage was characterized by determining sugars, ammoniacal nitrogen, acidity and pH at 0, 12, 24, 36, 48, 60 and 72 hours. Inoculation of sugar cane silage with Lactobacillus plantarum LPBR01 strain presented no significant results (p ≤ 0, 5) however, interaction between treatment and day (p ≤ 0, 5) could be observed for the levels of ADF. The Lactobacillus plantarum LPBR01 strain was not efficient to control the fermentation losses that occur in the silages of sugar cane at the concentration used in this study.


2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Ángela Mireya Rodríguez-Salgado ◽  
Luis Miguel Borrás-Sandoval ◽  
Carlos Eduardo Rodríguez-Molano

The fermentation indicators and compositional quality of food made with the solid-state fermentation of potatoes and carrots were analyzed in order to make use of foods that do not meet the requirements of consumers. Three treatments were formulated by varying the potato:carrot ratio mixed with wheat bran and corn cabbage. A microbiological analysis was performed at 0, 24 and 96 hours; the pH, crude protein (PC), ash (CZ), ethereal extract (EE), crude fiber (FC), neutral detergent fiber (FDN) and acid detergent fiber (FDA) were monitored at 0, 24, 48, 72 and 96 hours. Acidification was observed between sampling at 0 and 96 hours, with values ranging from 5.68 to 4.46, 5.73 to 4.46 and 5.69 to 4.33 for the treatments with the potato:carrot ratios 20:20, 25:25 and 30:30, respectively. Similarly, a reduction of dry matter (P<0.05) and PC increase (P<0.05) were observed from 20.1 to 26.7%, 21.1 to 27.1% and 25, 4 to 27.9%, respectively for the previous ratios. No significant statistical difference was found in the variables CZ, MS, EE, FDN, FDA or FC; a significant growth of lactic acid bacteria (LAB) counts and an absence of Salmonella were evident. The proportionality in the PC increase with the potato:carrot ratios was viable with the increase of the drying material, such as wheat bran and corn cabbage.


2016 ◽  
Vol 37 (3) ◽  
pp. 1441
Author(s):  
Rodrigo Luis Lemes ◽  
Cecilio Viega Soares Filho ◽  
Manoel Garcia Neto ◽  
Reges Heinrichs

The objective of this study was to evaluate the response of alfalfa (Medicago sativa L.) in the nutritional composition to the application of biofertilizers. The experiment was conducted with increasing doses of biofertilizers in a greenhouse at the Faculty of Veterinary Medicine / UNESP, Araçatuba - Sao Paulo, Brazil, from April to October 2010. The experimental design was completely randomized with six biofertilizer doses from cattle manure (0, 25, 50, 100, 200, and 400 m3 ha-1) and five replications. Cuts were performed, on average, every 27 days, 10 cm above the ground when 10% of the plants were flowering. Biofertilization had a positive significant impact on foliar nitrogen, potassium, calcium, magnesium, sulfur, and shoot iron concentrations. The values of crude protein, acid detergent fiber, and neutral detergent fiber did not differ between doses of biofertilizers. Biofertilization is a viable alternative for nutrition of this species, showing positive results in the nutritional composition of alfalfa. However, but long-term studies are necessary to assess the environmental impact of these fertilizers.


Sign in / Sign up

Export Citation Format

Share Document