scholarly journals Lactobacillus plantarum LPBR01 as inoculant in sugarcane silage

2019 ◽  
Vol 41 (1) ◽  
pp. 45708
Author(s):  
Fabiane Hoffmann ◽  
Maria Giovana Binder Pagnoncelli ◽  
Lilian Regina Rothe Mayer ◽  
Ana Carolina Fluck ◽  
Rodrigo Macagnan ◽  
...  

Sugar cane is highly productive (dry matter.hectare-1), but after ensiling process nutritional quality is affected, thus additives are needed to control or minimize losses. This study aimed to evaluate if Lactobacillus plantarum LPBR01 strain used as silage inoculant for sugar cane can control fermentation losses. Sugar cane samples (72) were divided in two treatments with three replicates, control (no Lactobacillus) and treatment silage with Lactobacillus (106 CFU g-1 of silage). Nutritional composition of samples in different periods of fermentation (0, 7, 15, 30 and 45 days) was estimated by determining levels of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM), mineral matter (MM) and acid detergent lignin (ADL). Fermentative profile of the silage was characterized by determining sugars, ammoniacal nitrogen, acidity and pH at 0, 12, 24, 36, 48, 60 and 72 hours. Inoculation of sugar cane silage with Lactobacillus plantarum LPBR01 strain presented no significant results (p ≤ 0, 5) however, interaction between treatment and day (p ≤ 0, 5) could be observed for the levels of ADF. The Lactobacillus plantarum LPBR01 strain was not efficient to control the fermentation losses that occur in the silages of sugar cane at the concentration used in this study.

2019 ◽  
Vol 40 (5Supl1) ◽  
pp. 2397
Author(s):  
Clésio dos Santos Costa ◽  
Rosane Cláudia Rodrigues ◽  
Ricardo Alves de Araújo ◽  
Francisco Naysson de Sousa Santos ◽  
Giovanne Oliveira Costa Sousa ◽  
...  

he aim of this study was to evaluate fermentation and chemical characteristics and the in situ degradability of sorghum silages enriched with dried Leucaena. The experiment was conducted as completely randomized design with four treatments (0 - control silage, silage without leucaena; 10.0, 20.0, or 30.0% inclusion of Leucaena) and five replicates, totaling 20 experimental units. The inclusion levels of leucaena influenced (P 0.05), with all silages having values above 90%. Gas loss decreased (P < 0.05) as the level of leucine increased in sorghum silage. A linear increase was observed for dry matter (P ? 0.0001) and crude protein (P = 0.0008) contents in response to Leucaena inclusion. There was a linear (P < 0.05) linear effect on neutral detergent fiber, hemicellulose and acid detergent fiber. As leucaena inclusion levels increased, for each 1% inclusion a reduction of 0.179 was observed; 0.059 and 0.119% for the values of neutral detergent fiber, hemicellulose and acid detergent fiber respectively. The inclusion of leucaena influenced (P < 0.05) the increase of silage lignin levels, probably due to the higher lignin content of the legume. The soluble fraction (a) of the silages increased along with the Leucaena inclusion levels, with highest values observed at the inclusion of 20 and 30% of the legume, respectively. Potential degradation (A) increased linearly with the levels of Leucaena added to the silage; the highest value for this variable was observed at 30% inclusion. Effective degradability (ED) increased up to the inclusion level of 20% (46.77%). Degradation rate (c) decreased markedly with the use of 30% Leucaena. The use of the forage part of Leucaena dried for three hours leads to reduced gas production, increased dry matter and crude protein contents, and decreased fiber contents. The degradability of dry matter increases up to the inclusion level of 20%.


2019 ◽  
Vol 33 (3) ◽  
pp. 182-194
Author(s):  
Wagner Sousa-Alves ◽  
João-Paulo S Rigueira ◽  
Marielly M Almeida-Moura ◽  
Diego L S De-Jesus ◽  
Flávio P Monção ◽  
...  

Background: Dry matter (DM) loss is high in sugarcane silage due to its high content of soluble carbohydrates which favors yeast growth and ethanol production, thus reducing the nutritional value of the preserved forage. Objective: To determine the effect of adding two types of urea at two concentration levels to sugarcane silage on the nutritional value and fermentative characteristics of the silage. Methods: A completely randomized 2×2+1 factorial design was used, based on two types of urea (conventional and protected), two urea concentration (0.5 and 1.0% of the natural matter), and a control (sugarcane silage with no additives). Six replicates were used. The mixture was placed in PVC silos and opened after 60 days. Results: There was no difference in DM content or effluent losses by the addition of urea. In regards to neutral detergent fiber, acid detergent fiber, and lignin, decreased levels were observed compared to the control, regardless of urea type. Urea type and concentration had an effect on ammoniacal nitrogen content, with higher values for conventional urea, but not affecting the pH. The addition of urea to sugarcane silage reduced gas losses and raised the levels of crude protein, ether extract, and effective degradability. Conclusion: Adding protected or conventional urea at 1% of natural matter at the time of sugarcane ensiling reduces gas losses and improves nutritional composition of the silage.


2007 ◽  
Vol 87 (4) ◽  
pp. 623-629 ◽  
Author(s):  
A. F. Mustafa ◽  
J. C. F. García ◽  
P. Seguin ◽  
O. Marois-Mainguy

A study was conducted to determine the effects of forage soybean cultivar on chemical composition, ensiling characteristics, and ruminal degradability of silage. Two cultivars of forage soybean (Kodiak and Mammouth) were field-grown, harvested at the R6 stage, and ensiled in mini-silos (n = 3) for 0, 2, 4, 8, 16 and 45 d. Two ruminally fistulated cows were used to determine in situ ruminal nutrient degradabilities of the 45-d silages. Both cultivars went through slow fermentation as indicated by a gradual decline in pH up to day 45 post-ensiling. Lactic acid concentration increased throughout ensiling and was higher for Mammouth than Kodiak except at day 45 post-ensiling. Analysis of the 45-d silages showed that Mammouth contained higher neutral detergent fiber (NDF, 490 vs. 444 g kg-1), acid detergent fiber (371 vs. 353 g kg-1) and acid detergent lignin (81 vs. 64 g kg-1) than Kodiak. However, crude protein (CP) concentration was higher for Kodiak than Mammouth. Mammouth silage had lower buffer soluble protein and higher neutral and acid detergent insoluble protein concentrations than Kodiak silage. Results of the in situ study indicated that Kodiak silage had greater ruminal dry matter (606 vs. 549 g kg-1), CP (828 vs. 752 g kg -1) and NDF (272 vs. 227 g kg-1) degradabilities than Mammouth. It was concluded that chemical composition and ruminal nutrient degradabilities of forage soybean silage were significantly influenced by cultivar. Key words: Soybean [Glycine max (L.) Merill.], silage, ensiling; forage quality, nutrient degradability


Author(s):  
Emrah Güngör ◽  
Aydın Altop ◽  
Guray Erener

The study was aimed to improve the nutritional composition of nettle (Utrica dioica L.) having positive effects on animals by solid-state fermentation. Aspergillus niger was used as a inoculant in fermentation of nettle. Before and after fermentation, crude protein (CP), ash, ether extract (EE), nitrogen-free extract (NFE), crude fiber (CF), hemicellulose (HC), neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) contents of nettle were determined. The ash, NFE and HC content of nettle were increased by fermentation. However, A. niger decreased the CF and EE in nettle. The results showed that solid-state fermentation with A. niger can be used to improve the nutritional composition of nettle.


2020 ◽  
Vol 18 (1) ◽  
pp. 11-20
Author(s):  
Ana Klinger ◽  
Diuly Falcone ◽  
Luiza Prates ◽  
Geni Toledo ◽  
Leila Silva

In developing countries, animal production is affected by the dependence on conventional ingredients or by its high cost, for which it is important to research agro-industrial by-products. Therefore, the purpose to this study was to evaluate the digestibility of diets for rabbits with sweet potato vines, in substitution to alfalfa hay. A biological essay was conducted to determine sweet potato vines digestibility, which took 12 days (5 for adaptation and 7 for collecting feces). A total of 27 rabbits received three different diets: reference (0SPV) and the ones with substitution of 10% (10SPV) and 15% (15SPV) of alfalfa hay for sweet potato vines. The coefficients of digestibility of dry matter were 51.47% (0SPV), 52.07% (10SPV) and 57.41% (15SPV); coefficients of digestibility of mineral matter, 15.17% (0SPV), 17.06% (10SPV) and 16.47% (15SPV); coefficients of digestibility of crude protein, 87.51% (0SPV), 84% (10SPV) and 83.79% (15SPV); coefficients of digestibility of neutral detergent fiber, 20.25% (0SPV), 19.65% (10SPV) and 24.48% (15SPV); coefficients of digestibility of acid detergent fiber, 16.06% (0SPV), 16.82% (10SPV) and 22.36% (15SPV). The conclusion is that sweet potato vines can replace at least 15% of alfalfa hay in growing meat rabbits diets.


Author(s):  
Aydın Altop

This study aimed to investigate the effect of solid-state fermentation on the nutritional composition of Agaricus bisporus mushroom stalk and cap. Four homogenous test samples were prepared from the fermented mushroom stalk (Fermented MS), unfermented mushroom stalk (MS), fermented mushroom cap (Fermented MC), and unfermented mushroom cap (MC); each was independently analyzed in triplicates, providing a total of 12 independent measurements of all treatments. Nutritional changes in the mushrooms were determined by analyzing crude protein (CP), ether extract (EE), crude ash (CA), crude fiber (CF), hemicellulose, neutral detergent fiber (NDF), acid detergent lignin (ADL), and acid detergent fiber (ADF) contents. The best improvement in nutritional composition was obtained from the MS with increasing the crude protein (35 %). The results related to other parameters are similar among all the treatments, except for CF. To conclude, the fermented MS can be considered as an alternative protein resource in animal nutrition.


2014 ◽  
Vol 38 (5) ◽  
pp. 497-505 ◽  
Author(s):  
Adriana de Souza Martins ◽  
Juliane Ribeiro de Oliveira ◽  
Marili Lopes Lederer ◽  
José Luís Moletta ◽  
Shivelly Los Galetto ◽  
...  

Due to the seasonal cycle of forage, the use of silage to feed animals provides nutrients throughout the year. However, its quality can be improved with the inclusion of additives and other products. Glycerol is a rich source of energy and present a high efficiency of utilization by animals. The purpose of this work was to evaluate the effect of glycerol inclusion on the chemical and fermentation characteristics of corn and sunflower silages. Two silage sources (maize and sunflower) were used and four levels of glycerol inclusion (0, 15, 30 and 45%) based on dry matter were carried out. The experimental design was completely randomized in a 2 x 4 factorial arrangement with five replications. The pH values and chemical composition of corn and sunflower silages were determined. In both silages there was increment of dry matter, non-fiber carbohydrates and total digestible nutrients (TDN) added to a reduction of crude protein, neutral detergent fiber and acid detergent fiber due to the glycerol inclusion. The corn silage required 45% glycerol to achieve the TDN level of the sunflower silage. The glycerol addition contributed to the increase in the nutritional value, offsetting loss of quality in the ensiling process.


2020 ◽  
Vol 9 (11) ◽  
pp. e95991110508
Author(s):  
Hosnerson Renan de Oliveira Santos ◽  
Mauro Pereira de Figueiredo ◽  
Ingrid Thalia Prado de Castro ◽  
Mário Henrique Melo e Lima ◽  
Luiza Maria Gigante Nascimento ◽  
...  

The objective was to evaluate agronomically nine sorghum-sudangrass hybrids (Sorghum bicolor × Sorghum sudanense) and the nutritive value of their respective hays. The experimental design used was randomized blocks in a 3 × 9 factorial scheme, with three replications. Three cuts were made: 42 days after germination, 29 days after regrowth, and 28 days after the second regrowth. All hybrids showed a higher number of plants ha–1 (P < 0.05) in the second cut. For the green matter and dry matter production of the hays, the values ranged from 49.16 to 62.07 t ha–1 and 9.07 to 11.43 t ha–1, respectively. There were no differences (P > 0.05) in dry matter, mineral matter, or ether extract. The hybrids 1013020, BRS 810, 1013016, and 1624F016 showed higher values of crude protein (CP) of 16.49, 16.17, 16.08, and 15.88%, respectively. Differences were observed (P < 0.05) in neutral detergent fiber (NDF) content. The hybrids 1013020, BRS 802, 1013021, 1013016, BRS 810, and 1624F006 presented lower levels of NDF (60.57, 60.74, 62.15, 62.37, 62.58, and 64.70%, respectively). The hybrids BRS 802, 1013016, BRS 810, and 1624F006, stood out above the others, presenting high forage yield and adequate nutritive value (CP content >13% and NDF content <65%).


Author(s):  
Ivone Rodrigues da Silva ◽  
Francirose Shigaki ◽  
Rosane Cláudia Rodrigues ◽  
Ana Paula Ribeiro Jesus ◽  
Clésio dos Santos Costa ◽  
...  

ABSTRACT The objective of this study was to evaluate the nutritive value of sugarcane silage with or without inoculation with P. acidipropionici or L. buchneri, over three fermentation periods. The experimental design was completely randomized in a 3 x 3 inoculant by fermentation period factorial arrangement (without inoculant, inoculant 1, inoculant 2; x three fermentation periods, 10, 60, 90 days). Values of pH, dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM) and lignin were determined and in situ DM degradability profiles were modelled for parameters a, b and c, potential degradation (A) and effective degradability (ED). The 90 day fermentation yielded a lower pH for both inoculants. There was an interaction between inoculant and fermentation period (P < 0.05) for DM content, with a reduction in silage DM without the additive at 90 days. The CP, HEM, ADF and lignin contents of sugarcane were not influenced by the treatments. The addition of P. acidipropionici provided the lowest NDF content at 10 days and presented a higher fraction a, potential degradation and ED. At 60 days, there was no variation in soluble fraction, the control silage showed a higher fraction b, higher potential degradation and ED. At 90 days of fermentation, L. buchneri silages presented a higher fraction a, degradation rate and DE and a higher b value was obtained in the silage without inoculant. Inoculants are effective in maintaining the silage DM content and nutritional value during prolonged fermentation periods.


2018 ◽  
Vol 70 (1) ◽  
pp. 29-36
Author(s):  
F.C.D. Araújo ◽  
M.G. Cruz ◽  
J.C.C. Balieiro ◽  
M.L. Menezes ◽  
C.G. Moreira ◽  
...  

ABSTRACT The aim of this study was to investigate the effect of odontoplasty on apparent digestibility of diet for horses, consumption time, and particle size of feces. Nine horses were used, aged 14.5±3.3 years and weightng 531±38.7kg. The diet consisted of 1.5% of live weight (LW) in Tifton 85 hay and 0.75% of LW in concentrate for both assays. The experiment consisted of two tests of apparent digestibility using the method of total collection, before and after the odontoplasty, where the consumption time of hay and concentrate was also evaluated and the particle size of the feces was analyzed. An effect (P<0.05) of odontoplasty on the apparent digestibility coefficients of dry matter, organic matter, crude protein, neutral detergent fiber and acid detergent fiber was observed. Effect of the consumption time for the concentrate (P<0.05) was observed, but not for the forage (P<0.05). There was more retention (P<0.05) of the fecal particles in the sieves of bigger granulometry. The odontoplasty increases the digestibility of dietary nutrients and feces particle size, without changing feces quality. Animals after the procedure have shorter concentrate consumption time.


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