Novel Health Supplement for Recovery of Alcohol Induced Adverse Effects and Associated Disorders: HepG2 Cells Study and Safety Dose Profiling on Rats

2021 ◽  
Vol 7 (5) ◽  
pp. 1-9
Author(s):  
Neelesh Kumar Nema ◽  
Keyword(s):  
Adverse Effects ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Plant Extracts ◽  
Hepg2 Cells ◽  
Human Hepatoma ◽  
Hepg2 Cell Line ◽  
Study Objective ◽  
Hepatoma Hepg2

The present study objective was to design and develop novel health-supplement formula from plant extracts and was to evaluate the formula for high episodic alcohol toxicities, and associated disorders against alcohol intoxicated and oxidative damaged Human Hepatoma HepG2 cell line.

Toxicity and metabolism of deoxynivalenol in human hepatoma (HepG2) cell line

2018 ◽  
Vol 295 ◽  
pp. S146-S147
Author(s):  
L. Radko ◽  
A. Tkaczyk ◽  
P. Jedziniak ◽  
S. Stypuła-Trębas ◽  
A. Posyniak
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Human Hepatoma ◽  
Hepg2 Cell Line ◽  
Hepatoma Hepg2

Genomic comparison of insecticides and herbicide in human hepatoma (HepG2) cell line

2010 ◽  
Vol 6 (4) ◽  
pp. 378-383 ◽  
Author(s):  
Yu Ri An ◽  
Seung-Jun Kim ◽  
Hey-Won Park ◽  
Moon-Ju Oh ◽  
Youn-Jung Kim ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Human Hepatoma ◽  
Hepg2 Cell Line ◽  
Genomic Comparison ◽  
Hepatoma Hepg2

Preparation of stabilized silver nanoparticles and study of their antimicrobial and cytotoxic activity on the human hepatoma HepG2 cell line

2019 ◽  
Vol 14 (5-6) ◽  
pp. 273-279
Author(s):  
M. A. Ananyan ◽  
A. G. Demchenko ◽  
V. S. Sadykova ◽  
A. V. Lyundup ◽  
T. I. Gromovykh ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Human Hepatoma ◽  
Hepg2 Cell Line ◽  
Hepatoma Hepg2

Effects of nimesulide and its metabolites or manufacturing intermediates on the viability and growth of the human hepatoma HepG2 cell line

Life Sciences ◽  
2001 ◽  
Vol 69 (25-26) ◽  
pp. 2965-2973 ◽  
Author(s):  
K.D. Rainsford ◽  
R.W. Seabrook ◽  
S. Spencer ◽  
A.T. Hewson
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Human Hepatoma ◽  
Hepg2 Cell Line ◽  
Hepatoma Hepg2

scholarly journals CYTOTOXICITY OF CLINACANTHUS NUTANS EXTRACTS ON HUMAN HEPATOMA (HepG2) CELL LINE

2016 ◽  
Vol 8 (10) ◽  
pp. 293 ◽  
Author(s):  
Hazrulrizawati Abd Hamid ◽  
Izzah Hayati Yahaya
Keyword(s):  
Hepg2 Cell ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Human Hepatoma ◽  
Hepg2 Cell Line ◽  
Flavonoid Content ◽  
Active Compounds ◽  
Clinacanthus Nutans ◽  
Hepatoma Hepg2

<p><strong>Objective</strong>:<strong> </strong><em>Clinacanthus nutans</em> is one of the herbs that has been used in Asia as a traditional medicine for the treatment of serious diseases. The aim of this study is to investigate the phytochemical constituents (flavonoids and phenolics) and cytotoxicity against human hepatoma (HepG2) cancer cell lines of <em>C. nutans </em>extracts.</p><p><strong>Methods</strong>:<strong> </strong>The fractions from <em>C. nutans</em> were extracted from hexane, methanol, chloroform and ethyl acetate by the solvent-solvent extraction method. The crude extracts (10 mg/ml) were tested against HepG2 cell lines using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Phytochemical screening was done to determine the total phenolic content (TPC) and total flavonoid content (TFC).</p><p><strong>Results</strong>:<strong> </strong>Methanol extract showed the strongest cytotoxic activity against HepG2 cell line with IC <sub>50</sub> of 43.9367μg/ml after 24 h of treatment compared to chloroform extract and ethyl acetate, 55.6112µg/ml and 62.0655µg/ml, respectively. Hexane extract formed the lowest cytotoxicity activity with IC<sub>50 </sub>of 68.3807µg/ml. Total phenolic content (TPC) was found to be highest in chloroform, which was 119.29 mg of gallic acid equivalent (GAE) and total flavonoid content (TFC), methanol was performed the highest value, which is 937.67 mg of butylated hydroxytoluene (BHT).</p><p><strong>Conclusion</strong>:<strong> </strong>Different active compounds present in the extracts may contribute different cytotoxicity effects of crude extracts. The relationship data of total phenolic, total flavonoid, and cytotoxic potential of <em>C. nutans</em>, indicates that these plants might contain valuable active compounds as a chemotherapeutic agent. Further investigations to elucidate the chemical structures of active compounds are necessary for potential compounds discovery in drugs.</p>

scholarly journals Differential Effects of Red Yeast Rice, Berberis Aristata and Morus Alba Extracts, Active Components of LopiGLIK®, on PCSK9 in HepG2 Cells

2018 ◽  
Author(s):  
Maria Giovanna Lupo ◽  
Chiara Macchi ◽  
Silvia Marchianò ◽  
Haixia Chen ◽  
Cesare Sirtori ◽  
...  
Keyword(s):  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Promoter Activity ◽  
Fatty Acid Synthase ◽  
Morus Alba ◽  
Red Yeast Rice ◽  
Mrna Levels ◽  
Hepg2 Cell Line ◽  
Red Yeast

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is the key regulator of low-density lipoprotein cholesterol (LDL-C) plasma levels. We previously observed that treatment of dyslipidemic subjects with nutraceutical combination containing red yeast rice (monacolin K 3.3 mg), Berberis aristata cortex extract (Berberine 531.25 mg) and Morus alba leaves extract (1-deoxynojirimycin 4 mg) (LopiGLIK&reg;) did not alter the plasma PCSK9 levels. Thus, the aim of the present study was to investigate the effect of these three components on PCSK9 expression in HepG2 cell line in relationship to their effects on LDL-C cellular uptake. HepG2 cell line were incubated with Berberis aristata cortex extract (BCE), red yeast rice (RYR) and Morus alba leaves extract (MLE) alone or in combination for 24 h. RYR (50 &micro;g/mL) increased PCSK9 protein expression (WB and ELISA assays), PCSK9 mRNA and its promoter activity. BCE (40 &micro;g/mL) reduced PCSK9 expression, mRNA levels and promoter activity. MLE determined a concentration-dependent inhibition of PCSK9 at mRNA and protein levels, with a maximal reduction at 1 mg/mL; no significant changes in PCSK9 promoter activity were found. MLE also downregulates the expression of fatty acid synthase and HMG-CoA reductase mRNA levels. The combination of RYR, BCE and MLE reduced PCSK9 at mRNA, protein, and promoter activity. Finally, this combination induced the LDL receptor and LDL-C uptake by HepG2 cells. In conclusion, the positive effect of MLE on PCSK9 supports the rational of using this nutraceutical combination to control hyperlipidemic conditions.

scholarly journals Evaluation of Antioxidant and Antidiabetic Potential of Polyherbal Drug Made with Three Herbs: An In vitro Cell Culture Model

2021 ◽  
Vol 14 (4) ◽  
pp. 1627-1635
Author(s):  
P. Chandrasekaran
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Plant Extracts ◽  
Antioxidant Properties ◽  
Protein Glycation ◽  
Gas Chromatography Mass Spectrometry ◽  
Individual Plant ◽  
Hepg2 Cell Line

In diabetes, the postprandial phase is characterized by a rapid and large increase in blood glucose levels, and the possibility that the postprandial “hyperglycemic spikes” may be relevant to the onset of cardiovascular complications has recently received much attention. Medicinal use of herbal medicine in the treatment and prevention of diseases including diabetes has a long history compared to conventional medicine. These plants have no side effects and many existing medicines are derived from the plants. Hence, the current investigation was planned to make a poly herbal drug (PHD) through Punica granatum (fruits), Illicium verum (flowers) and Nyctanthes arbor (leaves) and assess their antioxidant and antidiabetic activities in vitro and in HepG2 cell line. The respective plant methanolic extracts and PHD are exposed to phytochemical assessment and to discriminate the bioactive factors by Gas Chromatography–Mass Spectrometry. We evaluated the antioxidant properties 2, 2-diphenyl-1-picrylhydrazyl scavenging, hydrogen peroxide scavenging, thiobarbituric acid reactive substances and total antioxidant activity of individual plant extracts and the PHD. At the same time, In vitro and cell culture approaches were used to assess the anti-diabetic activity. The PHD has a higher concentration of secondary metabolites than individual plant extracts, according to our findings. On the other hand, we also notice that PHD demonstrated higher antioxidant capability and considerable in vitro glucose-lowering effects along with noteworthy inhibition of ɑ-amylase, glucosidase, lipase, dipeptidyl peptidase-IV, collagenase and protein glycation in HepG2 cell line. In conclusion, this study clearly demonstrated the significant antioxidant and antidiabetic activities of the PHD. Hence, PHD may be used as a potential source in the management of diabetes, hyperglycemia and the related state of oxidative stress.

Syringic acid triggers reactive oxygen species–mediated cytotoxicity in HepG2 cells

2019 ◽  
Vol 38 (6) ◽  
pp. 694-702 ◽  
Author(s):  
S Gheena ◽  
D Ezhilarasan
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Hepg2 Cells ◽  
Cytotoxic Effect ◽  
Reactive Oxygen ◽  
Syringic Acid ◽  
Oxygen Species ◽  
Hepg2 Cell Line ◽  
Gene Expressions

Hepatocellular carcinoma is the second most common cause of cancer death in the world and its incidence has dramatically increased worldwide in the past two decades. Syringic acid (SA) has been studied for its hepatoprotective, anti-inflammatory, immunomodulatory, free radical scavenging, and antioxidant activities. We aimed to evaluate the cytotoxic effect of SA against human hepatoma HepG2 cell line. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. HepG2 cells were treated with SA at concentration ranges of 25, 50, and 100 µM for 24 h. Reactive oxygen species (ROS) expression was investigated by dichlorofluorescein staining assay. Morphological changes of SA-treated HepG2 cells were evaluated by acridine orange (AO) and ethidium bromide (EB) dual staining. Apoptotic marker gene expressions were evaluated by qPCR. SA treatment caused significant cytotoxicity and liberation of ROS in HepG2 cells. AO and EB staining showed membrane blebbing and distortion in SA-treated cells. Apoptotic markers such as caspases 3 and 9, cytochrome c, Apaf-1, Bax, and p53 gene expressions were significantly increased upon SA treatment indicating the possibility of apoptosis induction in HepG2 cells. This treatment also caused significant downregulation of Bcl-2 gene expression. SA has a cytotoxic effect on human HepG2 cell line, and this might be a promising agent in anticancer research.

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