scholarly journals The Effects of Salt Stress on Ion Uptake, Accumulation of Compatible Solutes, and Leaf Osmotic Potential in Safflower, Chrysanthemum paludosum and Sea Aster.

1998 ◽  
Vol 67 (3) ◽  
pp. 426-431 ◽  
Author(s):  
Tomohiro Matsumura ◽  
Michio Kanechi ◽  
Noboru Inagaki ◽  
Susumu Maekawa
Keyword(s):  
Salt Stress ◽  
Osmotic Potential ◽  
Compatible Solutes ◽  
Ion Uptake ◽  
Leaf Osmotic Potential

Accumulation of Glycinebetaine in Chloroplasts Provides Osmotic Adjustment During Salt Stress

1986 ◽  
Vol 13 (5) ◽  
pp. 659 ◽  
Author(s):  
SP Robinson ◽  
GP Jones
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Salt Stress ◽  
Osmotic Adjustment ◽  
Osmotic Potential ◽  
Spinacia Oleracea ◽  
Resonance Spectroscopy ◽  
Leaf Osmotic Potential ◽  
Cellular Compartments ◽  
Isolated Chloroplasts

Glycinebetaine was determined in leaves and in isolated chloroplasts of spinach (Spinacia oleracea) by nuclear magnetic resonance spectroscopy. Some leakage of glycinebetaine from the chloroplasts occurred during the isolation so the concentration in chloroplasts in vivo could be up to 1.5 times higher than that measured in isolated chloroplasts. It was demonstrated that any contamination of the chloroplast preparations by glycinebetaine originating from other cellular compartments or from broken chloroplasts would have amounted to less than 10% of the measured values. Leaf osmotic potential of salt-stressed plants was -2.09 MPa compared to -0.91 MPa in non-stressed controls. This was accompanied by a sixfold increase in glycinebetaine content in the leaf but the levels of choline and proline were not increased. In chloroplasts isolated from control leaves the calculated glycinebetaine concentration was 26 mM which was 10-fold higher than the concentration in the leaf as a whole but only contributed 7% of the osmotic potential of the chloroplast. Chloroplasts from salt-stressed plants contained up to 300 mM glycinebetaine which was 20 times the concentration in the leaf as a whole. The glycinebetaine concentration in chloroplasts from salt-stressed leaves was equivalent to an osmotic potential of -0.75 MPa and this contributed 36% of the osmotic potential of the chloroplast and 64% of the decrease in osmotic potential induced by salt stress. At least 30-40% of the total leaf glycinebetaine was localized in the chloroplast. The results demonstrate that glycinebetaine accumulates in chloroplasts to provide osmotic adjustment during salt stress and provide support for the hypothesis that glycinebetaine is a compatible cytoplasmic solute which may be preferentially located in the cytoplasm of cells.

COMPARATIVE EVALUATION OF ENZYMATIC ACTIVITIES AND ION UPTAKE IN TWO GENOTYPES OF CLUSTER BEAN (Cyamopsis tetragonoloba L.) GROWN UNDER SALT STRESS

2020 ◽  
Vol 19 (1) ◽  
pp. 55-64
Author(s):  
Abu Bakr Umer Farooq ◽  
Muhammad Akram ◽  
Mehwish Zahid ◽  
Muhammad Tahir ◽  
Hafiz Mohkum Hammad ◽  
...  
Keyword(s):  
Salt Stress ◽  
Comparative Evaluation ◽  
Enzymatic Activities ◽  
Ion Uptake ◽  
Cyamopsis Tetragonoloba ◽  
Cluster Bean

scholarly journals Effect of Seed Inoculation on Alfalfa Tolerance to Water Deficit Stress

2017 ◽  
Vol 45 (1) ◽  
pp. 82-88
Author(s):  
Mahnaz ZAFARI ◽  
Ali EBADI ◽  
Sodabeh JAHANBAKHSH GODEHKAHRIZ
Keyword(s):  
Water Deficit ◽  
Osmotic Potential ◽  
Mycorrhizal Fungi ◽  
Soluble Sugars ◽  
Compatible Solutes ◽  
Membrane Stability ◽  
Forage Yield ◽  
Water Deficit Stress ◽  
Cell Membrane Stability ◽  
Seed Inoculation

Water deficit is one of the most important environmental stresses that adversely affect crop growth and production and mycorrhizal fungi and symbiotic bacteria have important role in resistance to drought stress. The effect of biofertilizers on alfalfa stress tolerance was studied at the greenhouse condition. Treatments comprised three water-deficit stresses (35%, 55% and 75% of field capacity) and four seeds inoculations (Glomus mosseae, Sinorhizobium meliloti, G. mosseae + S. meliloti and non-inoculated). Water-deficit stress decrease cell membrane stability (39%), total Chl (24.05%), carotenoid (35.55%), quantum yield (50.64%) and forage yield (28.20%), while increased the proline and soluble sugars content (68.55 and 46.53% respectively) and osmotic potential (45.84%). The inoculation of seeds increased the capability of the plants in counteracting the stress, so that the production of compatible solutes was increased and the photosynthetic indices, proline, osmotic potential, membrane stability and forage yield were improved by seed inoculation. Mycorrhiza improved photosynthetic indexes and proline, but bacteria had more efficacy on membrane stability and forage yield. However, double inoculation due to the synergistic effect of mycorrhiza and Sinorhizobium, had the greatest effect than Solitary inoculation. Our results suggest that biofertilized alfalfa plants were better adapted than non- biofertilized ones to cope with water deficit.

scholarly journals Modulation of ion uptake in tomato (Lycopersicon esculentum L.) plants with exogenous application of calcium under salt stress condition

Poljoprivreda ◽  
2016 ◽  
Vol 22 (2) ◽  
pp. 40-49 ◽  
Author(s):  
Khursheda Parvin ◽  
◽  
Kamal Uddin Ahamed ◽  
Mohammad Mahbub Islam ◽  
Nazmul Haque
Keyword(s):  
Salt Stress ◽  
Lycopersicon Esculentum ◽  
Stress Condition ◽  
Ion Uptake ◽  
Salt Stress Condition ◽  
Exogenous Application

Root osmotic potential and length for two maize lines differing in leaf osmotic potential

2019 ◽  
Vol 33 (4) ◽  
pp. 429-444 ◽  
Author(s):  
Amber Beseli ◽  
Antonio J. Hall ◽  
Anju Manandhar ◽  
Thomas R. Sinclair
Keyword(s):  
Osmotic Potential ◽  
Leaf Osmotic Potential

scholarly journals Sugar/osmoticum levels modulate differential abscisic acid-independent expression of two stress-responsive sucrose synthase genes in Arabidopsis

1999 ◽  
Vol 344 (2) ◽  
pp. 503-509 ◽  
Author(s):  
Annabelle DÉJARDIN ◽  
Lubomir N. SOKOLOV ◽  
Leszek A. KLECZKOWSKI
Keyword(s):  
Signal Transduction ◽  
Abscisic Acid ◽  
Cold Stress ◽  
Osmotic Potential ◽  
Sucrose Synthase ◽  
Cellular Response ◽  
Expression Profiles ◽  
Soluble Sugars ◽  
Leaf Osmotic Potential ◽  
Detached Leaves

Sucrose synthase (Sus) is a key enzyme of sucrose metabolism. Two Sus-encoding genes (Sus1 and Sus2) from Arabidopsis thaliana were found to be profoundly and differentially regulated in leaves exposed to environmental stresses (cold stress, drought or O2 deficiency). Transcript levels of Sus1 increased on exposure to cold and drought, whereas Sus2 mRNA was induced specifically by O2 deficiency. Both cold and drought exposures induced the accumulation of soluble sugars and caused a decrease in leaf osmotic potential, whereas O2 deficiency was characterized by a nearly complete depletion in sugars. Feeding abscisic acid (ABA) to detached leaves or subjecting Arabidopsis ABA-deficient mutants to cold stress conditions had no effect on the expression profiles of Sus1 or Sus2, whereas feeding metabolizable sugars (sucrose or glucose) or non-metabolizable osmotica [poly(ethylene glycol), sorbitol or mannitol] mimicked the effects of osmotic stress on Sus1 expression in detached leaves. By using various sucrose/mannitol solutions, we demonstrated that Sus1 was up-regulated by a decrease in leaf osmotic potential rather than an increase in sucrose concentration itself. We suggest that Sus1 expression is regulated via an ABA-independent signal transduction pathway that is related to the perception of a decrease in leaf osmotic potential during stresses. In contrast, the expression of Sus2 was independent of sugar/osmoticum effects, suggesting the involvement of a signal transduction mechanism distinct from that regulating Sus1 expression. The differential stress-responsive regulation of Sus genes in leaves might represent part of a general cellular response to the allocation of carbohydrates during acclimation processes.

Proline Analogues in Melaleuca Species: Response of Melaleuca lanceolata and M. uncinata to Water Stress and Salinity

1987 ◽  
Vol 14 (6) ◽  
pp. 669 ◽  
Author(s):  
BP Naidu ◽  
GP Jones ◽  
LG Paleg ◽  
A Poljakoff-Mayber
Keyword(s):  
Water Stress ◽  
Osmotic Adjustment ◽  
Osmotic Potential ◽  
Compatible Solutes ◽  
Species Response ◽  
Laboratory Conditions ◽  
Relative Water ◽  
Response To Water ◽  
First Time

Fifteen species of Melaleuca and two species of Callistemon from the field were examined to determine whether they accumulated nitrogen-containing compatible solutes and, if so, which. In addition to L-proline, N-methyl-L-proline (MP) (isolated for the first time from plants), trans-4-hydroxy-N-methyl- L-proline (MHP), and N, N'-dimethyl-trans-4-hydroxy-L-proline (DHP) were found in various combinations in the 15 Melaleuca species. M. lanceolata seedlings were subjected to water or salinity stress and M. uncinata to water stress under laboratory conditions. In both species significant reductions in leaf water potential (Ψw), osmotic potential (Ψs), turgor potential (Ψp), and relative water content (RWC) were observed in response to water stress. Salinised M. lanceolata plants showed considerable osmotic adjustment and maintained Ψp comparable to that of control plants; salinity, however, decreased RWC. In response to the imposed stresses under laboratory conditions, proline and MHP levels in M. lanceolata, and MHP and DHP levels in M. uncinata, increased. In addition to possible protective or osmotic roles in vivo, these proline analogues may be useful in chemotaxonomic investigations of Melaleuca species.

Temporal water use by two maize lines differing in leaf osmotic potential

Crop Science ◽  
2020 ◽  
Vol 60 (2) ◽  
pp. 945-953 ◽  
Author(s):  
Amber L. Beseli ◽  
Avat Shekoofa ◽  
Mujahid Ali ◽  
Thomas R. Sinclair
Keyword(s):  
Water Use ◽  
Osmotic Potential ◽  
Leaf Osmotic Potential
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