scholarly journals Nilai VFA dan NH3 Rumput Alam Padang Penggembalaan Kecamatan Haharu Kabupaten Sumba Timur

2021 ◽  
Vol 5 (1) ◽  
pp. 8-12
Author(s):  
Marselinus Hambakodu ◽  
Elvis Pati Ranja ◽  
Made Adi Sudarma
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Steam Distillation ◽  
Rumen Fluid ◽  
Descriptive Analysis ◽  
Survey Method ◽  
Bothriochloa Ischaemum ◽  
Natural Grass ◽  
The Mean

This study aims to determine the value of volatile fatty acids (VFA) and ammonia (NH3) of natural grass in grazing fields in vitro. The study used a direct survey method in the field. Natural grass was tested in vitro using Bali cattle rumen fluid. The data obtained were analyzed using descriptive analysis based on the mean and standard deviation. Natural grass consisted of Heteropogon insignis, Bracharia decumbens, Bothriochloa ischaemum. The measurement of the value of VFA uses steam distillation, while the method of measuring the value of NH3 uses Conway micro diffusion. The results showed that Bracharia decumbens grass had a total VFA value of 74.48 mM and an NH3 value of 8.50 mM which was higher than Heteropogon insignis grass (total VFA 65.79 mM and NH3 6.67 mM), and Bothriochloa ischaemum grass (total VFA 60.64 mM and NH3 5.34 mM). The conclusion of this study was based on the value of VFA and NH3, Bracharia decumbens grass was a natural grass that may be cultivated for ruminants.

scholarly journals Essential oils effect on rumen fermentation and biohydrogenation under in vitro conditions

2014 ◽  
Vol 59 (No. 10) ◽  
pp. 450-459 ◽  
Author(s):  
M. Gunal ◽  
A. Ishlak ◽  
A.A. AbuGhazaleh ◽  
W. Khattab
Keyword(s):  
Fatty Acids ◽  
Essential Oils ◽  
Dose Level ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Buffer Solution ◽  
Cinnamon Oil ◽  
Tea Tree

The effects of adding essential oils (EO) at different levels (125, 250, 500 mg/l) on rumen fermentation and biohydrogenation were examined in a rumen batch culture study. Treatments were: control without EO (CON), control with anise oil (ANO), cedar wood oil (CWO), cinnamon oil (CNO), eucalyptus oil (EUO), and tea tree oil (TEO). Essential oils, each dissolved in 1 ml of ethanol, were added to the culture flask containing 40 ml of buffer solution, 2 ml of reduction solution, 10 ml of rumen fluid, 25 mg of soybean oil, and 0.5 g of the diet. After 24 h of incubation in a water batch at 39°C, three samples were collected from each flask and analyzed for ammonia-N, volatile fatty acids (VFA), and fatty acids (FA). Expect for CNO, the proportions of acetate, propionate, and acetate to propionate ratios were not affected (P > 0.05) by EO addition. Addition of CWO, CNO, and TEO reduced total VFA concentrations (P < 0.05) regardless of dose level. The ammonia-N concentration was greater in cultures incubated with EO regardless of dose level. Compared with the CON, the concentrations of C18:0 and trans C18:1 were reduced (P < 0.05) with EO addition regardless of dose level. Compared with the CON, the concentration of linoleic acid was greater (P < 0.05) when EO were added at 500 mg/l. EO tested in this study had no effects on VFA profile but significantly reduced the formation of biohydrogenation products (C18:0 and trans C18:1).

scholarly journals Multiple-factor associative effects of peanut shell combined with alfalfa and concentrate determined by in vitro gas production method

2019 ◽  
Vol 64 (No. 8) ◽  
pp. 352-360
Author(s):  
Jiu Yuan ◽  
Xinjie Wan
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Production Method ◽  
Ammonia Nitrogen ◽  
Gas Production ◽  
Peanut Shell ◽  
Dry Matter Digestibility ◽  
In Vitro Gas Production

The associative effects (AE) between concentrate (C), peanut shell (P) and alfalfa (A) were investigated by means of an automated gas production (GP) system. The C, P and A were incubated alone or as 40 : 60 : 0, 40 : 45 : 15, 40 : 30 : 30, 40 : 15 : 45, 40 : 0 : 60 and 30 : 70 : 0, 30 : 55 : 15, 30 : 40 : 30, 30 : 25 : 45, 30 : 10 : 60, 30 : 0 : 70 mixtures where the C : roughage (R) ratios were 40 : 60 and 30 : 70. Samples (0.2000 ± 0.0010 g) of single feeds or mixtures were incubated for 96 h in individual bottles (100 ml) with 30 ml of buffered rumen fluid. GP parameters were analysed using a single exponential equation. After incubation, the residues were used to determine pH, dry matter digestibility (DMD), organic matter digestibility (OMD), volatile fatty acids (VFA) and ammonia nitrogen (NH<sub>3</sub>-N) of the incubation fluid, and their single factor AE indices (SFAEI) and multiple-factors AE indices (MFAEI) were determined. The results showed that group of 30 peanut shell had higher SFAEI of GP<sub>48 h</sub>, DMD, OMD and total volatile fatty acids (p &lt; 0.05) and MFAEI (p &lt; 0.05) than groups 60, 45 and 0 when C : R was 40 : 60. The group of 10 peanut shell showed higher SFAEI of GP<sub>48 h</sub>, DMD and OMD (p &lt; 0.05) than groups 70, 55 and 40 and MFAEI (p &lt; 0.01) when C : R was 30 : 70. It is concluded that optimal SFAEI and MFAEI were obtained when the C : P : A ratios were 40 : 30 : 30 and 30 : 10 : 60.

scholarly journals Excretion of purine derivatives by ruminants: recycling of allantoin into the rumen via saliva and its fate in the gut

1990 ◽  
Vol 63 (2) ◽  
pp. 197-205 ◽  
Author(s):  
X. B. Chen ◽  
F. D. DeB. Hovell ◽  
E. R. ØRskov
Keyword(s):  
Fatty Acids ◽  
Uric Acid ◽  
Urinary Excretion ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Purine Derivatives ◽  
Complete Destruction ◽  
Series Of Experiments ◽  
Micro Organisms

The saliva of sheep was shown to contain significant concentrations of uric acid (16 (sd) 4.5) μmol/l) and allantoin (120 (sd 16.4) μmol/l), sufficient to recycle purine derivatives equivalent to about 0.10 of the normal urinary excretion. When allantoin was incubated in vitro in rumen fluid, it was degraded at a rate sufficient to ensure complete destruction of recycled allantoin. In a series of experiments in which allantoin was infused into the rumen of sheep fed normally, or into the rumen or abomasum of sheep and the rumen of cattle completely nourished by intragastric infusion of volatile fatty acids and casein, no additional allantoin was recovered in the urine. These losses were probably due to the degradation of allantoin by micro-organisms associated with the digestive tract. It is concluded that all allantoin and uric acid recycled to the rumen via saliva will be similarly degraded. Therefore, the use of urinary excretion of purine derivatives as an estimator of the rumen microbial biomass available to ruminants will need to be corrected for such losses.

scholarly journals In vitro assessment of ruminal fermentation, digestibility and methane production of three species of Desmanthus for application in northern Australian grazing systems

2018 ◽  
Vol 69 (8) ◽  
pp. 797 ◽  
Author(s):  
Sophie Vandermeulen ◽  
Sultan Singh ◽  
Carlos Alberto Ramírez-Restrepo ◽  
Robert D. Kinley ◽  
Christopher P. Gardiner ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Organic Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Northern Australia ◽  
Rhodes Grass ◽  
Ch4 Production ◽  
Grass Hay

Three species of Desmanthus adapted to the heavy clay soils of northern Australia were studied to determine their nutritive value and effects on in vitro fermentation with rumen fluid, compared with Rhodes grass (Chloris gayana) hay. Leaves and stems of D. leptophyllus cv. JCU 1, D. virgatus cv. JCU 2 and D. bicornutus cv. JCU 4 were collected in summer, winter and spring of 2014 and analysed for chemical composition. Apparent digestibility as in vitro organic matter digestibility (IVD-OM) and fermentation parameters including methane (CH4) production were measured during 72-h fermentations using rumen fluid from steer donors grazing tropical grasses and legumes. Desmanthus bicornutus was on average more digestible than both D. leptophyllus and D. virgatus at 24, 48 and 72 h of incubation. This species also demonstrated an anti-methanogenic potential, in particular when harvested in summer with a reduction in CH4 production of 26% compared with Rhodes grass hay after 72 h of incubation. At this time point, D. leptophyllus produced higher volatile fatty acids (VFA per g of organic matter fermented) compared with the other forages. This legume also reduced the CH4 production up to 36% compared with the Rhodes grass hay reference. However, D. leptophyllus showed lower IVD-OM. Overall, Desmanthus species produced lower in vitro CH4 and lower volatile fatty acids concentration compared with the reference grass hay. These effects may be due to presence of secondary compounds such as hydrolysable tannins, condensed tannins and/or their combination in Desmanthus species. The IVD-OM was influenced by the season after 72 h of incubation; the digestibility was higher in plants collected in spring. This study suggests that contrasting fermentative profiles in Desmanthus cultivars may offer the opportunity to reduce the greenhouse gas contribution of the beef industry. The next step in demonstration of these promising in vitro results is demonstration of Desmanthus in vivo as proof of concept confirming the productivity and CH4 reduction ability of these legumes in the pastoral systems of northern Australia.

Effects of forage neutral detergent fibre and time after feeding on medial and ventral rumen pH and volatile fatty acids concentration in heifers fed highly digestible grass/clover silages

2017 ◽  
Vol 57 (1) ◽  
pp. 129 ◽  
Author(s):  
A. K. S. Schulze ◽  
A. C. Storm ◽  
M. R. Weisbjerg ◽  
P. Nørgaard
Keyword(s):  
Fatty Acids ◽  
Organic Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Growth Stages ◽  
Neutral Detergent Fibre ◽  
Rumen Epithelium ◽  
Rumen Ph

The major microbial fermentation of forages and production of volatile fatty acids (VFA) takes place in the medial part of the rumen, whereas the absorption of VFA occurs through the rumen epithelium, for example the ventral sac. The objective was to study effects of forage neutral detergent fibre (NDF) content and time after feeding on the medial to ventral VFA and pH gradient as well as rumen motility in the rumen of heifers fed grass/clover silages. Four silages were harvested at different growth stages with NDF contents of 31–45% of DM and in vitro organic matter digestibilities of 75–82% and fed to four rumen-fistulated Jersey heifers at 90% of ad libitum level in a Latin square design, with half the ration fed at 0800 hours and 1530 hours. Rumen fluid was sampled hourly from 0730 hours to 1530 hours in the medial and ventral rumen, and analysed for pH and concentrations of VFA, L-lactic acid, and ammonia to assess ruminal chemical gradient. Reticular contractions were continuously recorded by a pressure transducer. Time relative to feeding affected rumen parameters as pH was generally lower and VFA content greater in medial compared with ventral rumen fluid. Greater NDF content of the silage caused lower VFA concentration and higher pH in the rumen mat, and therefore the gradient diminished at greater NDF content in the silages; an effect probably caused by reduced organic matter digestibility rather than digesta NDF properties. This study therefore suggests that VFA production decreased with greater NDF content of forages, whereas intra-ruminal equilibration increased.

The production and absorption of volatile fatty acids from the rumen of the sheep

1968 ◽  
Vol 19 (5) ◽  
pp. 791 ◽  
Author(s):  
GJ Faichney
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Volatile Fatty Acids ◽  
Metabolizable Energy ◽  
The Other ◽  
In Vitro Fermentation ◽  
The Mean ◽  
The Difference

Experiments are reported in which sheep were given roughage diets or a high concentrate diet and the VFA absorbed from the rumen were estimated by an in vitro fermentation procedure. The VFA absorbed were compared with the digestible and metabolizable energy intakes of the sheep, determined in digestibility trials, for each diet. For a lucerne diet, a straw diet, and the high concentrate diet the proportions of the digested energy absorbed as VFA were 33.6, 42.4, and 33.2% respectively. On the lucerne diet, the difference between the mean molar proportions of the VFA absorbed and the mean molar proportions of the VFA in the rumen approached significance for acetic acid (P < 0.10) and was highly significant for butyric acid (P < 0.01). The differences were not significant for the other diets.

Fermentation in the Rumen of the Sheep

1951 ◽  
Vol 28 (1) ◽  
pp. 83-90
Author(s):  
F. V. GRAY ◽  
A. F. PILGRIM
Keyword(s):  
Fatty Acids ◽  
Propionic Acid ◽  
Butyric Acid ◽  
Volatile Fatty Acids ◽  
Rumen Fluid

1. Analyses of the rumen fluid of sheep fed on wheaten hay and on lucerne hay showed that characteristic changes take place in the composition of the mixture of volatile fatty acids in the rumen throughout the day. 2. The changes conform closely to those predicted from the composition of the mixture of fatty acids produced from the same two fodders in vitro. They support the view that propionic acid is relatively more rapidly absorbed than either acetic or butyric acid, and that the fermentation of these fodders in the rumen produces a mixture of the acids in which propionic acid forms a larger proportion than it does in the rumen fluid.

scholarly journals Passage of protozoa and volatile fatty acids from the rumen of the sheep and from a continuous in vitro fermentation system

1974 ◽  
Vol 32 (2) ◽  
pp. 341-351 ◽  
Author(s):  
R. A. Weller ◽  
A. F. Pilgrim
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
In Vitro Fermentation ◽  
Fermentation System

1. A procedure for sampling digesta from within the omasal canal of sheep given a variety of roughage diets was used to enable comparison to be made of the composition of effluent from the reticulo-rumen with that of rumen fluid.2. Concentrations of protozoa in effluents, relative to a soluble marker continuously infused intraruminally, were usually less than 20% of corresponding rumen fluid concentrations. It was estimated that the amount of protozoal nitrogen leaving the rumen represented less than 2% of dietary N.3. Passage of volatile fatty acids (VFA) from the rumen in effluent was less than 75% of that indicated by rumen concentrations.4. A continuous, in vitro fermentation system was developed, in which outputs of protozoa were comparable with in vivo outputs.

scholarly journals Evaluating the effects of six essential oils on fermentation and biohydrogenation in in vitro rumen batch cultures

2013 ◽  
Vol 58 (No. 6) ◽  
pp. 243-252 ◽  
Author(s):  
M. Gunal ◽  
A. Ishlak ◽  
AbughazalehAA
Keyword(s):  
Fatty Acids ◽  
Essential Oils ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Batch Cultures ◽  
Citronella Oil ◽  
Modify Acetate ◽  
Trans Fa

The effects of six essential oils (EO) on rumen fermentation and biohydrogenation were evaluated under in vitro conditions. Three doses (125, 250, and 500 mg/l) of EO were evaluated using in vitro 24 h batch culture of rumen fluid with a 55 : 45 forage : concentrate diet. Treatments were control (CON), control with Siberian fir needle oil (FNO), citronella oil (CTO), rosemary oil (RMO), sage oil (SAO), white thyme oil (WTO), and clove oil (CLO). Treatments were incubated in triplicate in 125 ml flasks containing 500 mg of finely ground total mixed ration (TMR), 25 mg of soybean oil, 10 ml of the strained ruminal fluid, 40 ml of media, and 2 ml of reducing solution. After 24 h, the pH was determined and samples were collected to analyze ammonia N, volatile fatty acids (VFA), and fatty acids (FA). Cultures pH was not affected by EO averaging 6.6&nbsp;&plusmn; 0.2. In general, high EO doses reduced the total VFA concentration except for SAO and RMO. Relative to CON, all EO decreased (P &lt; 0.05) ammonia N concentrations except for the highest dose of WTO. Except for SAO, EO did not modify acetate to propionate ratio. Relative to CON, the addition of CTO and FNO increased (P &lt; 0.05) the proportions of isobutyrate and decreased (P &lt; 0.05) the proportions of valerate and isovalerate. The concentrations (mg/culture) of C18:0 and C18:1 trans FA decreased (P &lt; 0.05) with CTO, FNO, RMO, and SAO relative to CON. Most tested EO in this study had little to no effects on conjugated linoleic acids (CLA), and linoleic and linolenic acids concentrations. In conclusion, results from this study showed that except for effects on ammonia N, EO tested in this study had moderate effects on rumen fermentation. The reduction in the formation of trans FA and C18:0 with some EO may indicate shifts in the biohydrogenation pathways toward the formation of other unidentified intermediate FA.

Sign in / Sign up

Export Citation Format

Share Document