scholarly journals Effects of grape seed extract on properties of type I collagen scaffolds

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Claudio Fernandes Garcia ◽  
Virginia C. A. Martins ◽  
Ana M. G. Plepis
Keyword(s):  
Type I Collagen ◽  
Crosslinking Agent ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Type I ◽  
Scanning Calorimetry ◽  
Phosphate Buffered Saline ◽  
Collagen Suspension

 To obtain a material with potential for use in tissue engineering, anionic collagen was obtained from porcine serosa (S) and bovine tendon (T) by alkaline hydrolysis for 72h. Part of this collagen was mixed with water to obtain 4 % (weight/weight) collagen suspension and part was solubilized in acetic acid pH 3.5 to obtain 1.5% (w/w) gel. The suspensions were mixed with their respective gels (2:1) (suspension: gel) and grape seed extract, whose main product is proanthocyanidin, was added at concentrations of 0.03% and 0.5%, thus obtaining the scaffolds SC (serosa collagen suspension and gel), TC (tendon collagen suspension and gel), SCP003 (SC with 0.03% extract), TCP003 (TC with 0.03% extract), SCP05 (SC with 0.5% extract added) and TCP05 (TC with 0.5% extract). The materials were analyzed by differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and characterized by phosphate buffered saline absorption assay and in vitro biological stability assay. By DSC it is observed that the addition of 0.5% of extract increases the denaturation temperature (Td) of collagen, indicating that at this concentration the extract acts as polymer crosslinking agent. SEM shows disorganized cross-section pores in all scaffolds, not exceeding 130 μm. Absorption and degradation assays indicated that the addition of 0.5% extract increases the absorption of phosphate buffered saline (PBS) by the scaffolds and decreases the degradation percentage by collagenase. These results suggests that the scaffolds can be used for different applications, e.g. as hemostatic agent.  

Grape Seed Extract Complex (FFM-GS) Inhibits Postmenopausal Symptoms: In Vitro and In Vivo S

2019 ◽  
Vol 48 (3) ◽  
pp. 297-305
Author(s):  
Minhee Lee ◽  
Da-Eun Nam ◽  
Soo-Jeung Park ◽  
Dakyung Kim ◽  
Jeong-Moon Yun ◽  
...  
Keyword(s):  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Postmenopausal Symptoms

scholarly journals Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study

2012 ◽  
Vol 13 (4) ◽  
pp. 425-430 ◽  
Author(s):  
Shiny Benjamin ◽  
Roshni LNU ◽  
Sabeena Susan Thomas ◽  
Mohan Thomas Nainan
Keyword(s):  
Laser Scanning ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Confocal Laser Scanning Microscope ◽  
Seed Extract ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Caries Lesions ◽  
Calcium Glycerophosphate

ABSTRACT Objective Remineralization is an effective treatment that may stop or reverse early tooth decay. Grape seed extract (GSE) is the potential remineralizing agent under investigation. Materials and methods Sound human tooth sections were obtained from the cervical portion of the root and stored in demineralizing solution at 37°C for 96 hours to induce artificial root caries lesions. The sections were divided into four treatment groups including 6.5% grape seed extract, sodium monofluorophosphate (220 ppm) with 0.05% calcium glycerophosphate, 0.5% calcium glycerophosphate and control (no treatment). An in vitro pH cycling model was used to cycle the demineralized specimens through treatment solutions, acidic buffer and neutral buffer for 8 days at 6 cycles per day. Subsequently, they were evaluated using confocal laser scanning microscope. Data were analyzed using analysis of variance (p < 0.05). Results GSE revealed less demineralization and more remineralization compared with other groups. Conclusion GSE promotes remineralization of artificial root caries lesions. Clinical significance The search for the perfect remineralizing agent continues to this day. GSE could be a welcome addition to the remineralization armamentarium. Abbreviations and acronyms GSE: Grape seed extract; ppm: Parts per million; CaGP: Calcium glycerophosphate; CLSM: Confocal laser scanning microscope; ANOVA: Analysis of variance; PA: Proanthocyanidin; CEJ: Cementoenamel junction; mM: Millimole; CaCl2.2H2O: Calcium chloride dihydrate; KH2PO4: Potassium dehydrate phosphate; K2HPO4: Dipotassium phosphate; dH2O: Deionized water; w/v: Weight by volume; ROD: Relative optical density; nm: Nanometer; SD: Standard deviation. How to cite this article Benjamin S, Roshni, Thomas SS, Nainan MT. Grape Seed Extract as a Potential Remineralizing Agent: A Comparative in vitro Study. J Contemp Dent Pract 2012;13(4):425-430.

scholarly journals Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1β activities before and after oral consumption

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Elsa Mével ◽  
Christophe Merceron ◽  
Claire Vinatier ◽  
Stéphanie Krisa ◽  
Tristan Richard ◽  
...  
Keyword(s):  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Oral Consumption ◽  
Before And After ◽  
Post Traumatic

scholarly journals Grape Seed Extract Inhibits In vitro and In vivo Growth of Human Colorectal Carcinoma Cells

2006 ◽  
Vol 12 (20) ◽  
pp. 6194-6202 ◽  
Author(s):  
Manjinder Kaur ◽  
Rana P. Singh ◽  
Mallikarjuna Gu ◽  
Rajesh Agarwal ◽  
Chapla Agarwal
Keyword(s):  
Colorectal Carcinoma ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Carcinoma Cells ◽  
Human Colorectal Carcinoma ◽  
In Vivo Growth

scholarly journals EFFECT OF GRAPE SEED EXTRACT ON REMINERALIZATION OF ARTIFICIAL CARIES: AN IN-VITRO STUDY

2016 ◽  
Vol 9 (5) ◽  
pp. 174 ◽  
Author(s):  
Rubel M ◽  
Prashant Gm ◽  
Naveen Kumar Pg ◽  
Sushanth Vh ◽  
Mohamed Imranulla ◽  
...  
Keyword(s):  
Dental Caries ◽  
In Vitro Study ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
T Test ◽  
Vitro Study ◽  
Saline Control ◽  
Group Comparison

ABSTRACTObjective: Grape seeds are gaining more importance because the “Proanthocyanidins” present in them have excellent anticaries, remineralizing, andantibacterial properties. Although many preventive measures have been tried, dental caries remains one of the most prevalent diseases in humans.The ultimate goal of any intervention is the preservation of tooth structure, prevention of lesion progression and inducing remineralization. Thepresent in-vitro study was done to investigate the effect of grape seed extract (GSE) on artificial enamel caries in human teeth.Methods: A total of 50 sound premolars extracted from patients undergoing orthodontic treatment were used in the study. The teeth were cleanedand were immersed in a demineralizing solution for 96 hrs at 37°C and 50% relative humidity to create artificial lesions. The samples were randomlydivided into two groups of 25 each. Half of the samples were immersed in the GSE solution (test) and the other half in saline (control), respectively, for8 days. The samples were measured by microhardness tester to obtain values in Vickers hardness scale. Data were entered into Microsoft Excel andwere analyzed using independent t-test for inter-group comparison and paired t-test for intra-group comparison using SPSS version 21.Results: The mean±SD microhardness values after remineralization for the test and control groups were 357.56±5.53 and 337.60±6.38. GSE groupsignificantly increased the microhardness of the lesions (p≤0.001).Conclusion: GSE is a potential remineralizing agent and can be used to prevent progression of dental caries.Keywords: Grape seed extract, Remineralization, Dental caries.

Grape Seed Extract Exerts Abhesive Effect Against Staphylococcus aureus: In vitro Study

2012 ◽  
Vol 7 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Marwan S.M. Al-Ni ◽  
Rana Abd-ul-Karim Rasheed ◽  
Shama Mohamed Jawad Saadaldin
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Study ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Vitro Study

scholarly journals Antilisterial Effects of Gravinol-S Grape Seed Extract at Low Levels in Aqueous Media and Its Potential Application as a Produce Wash

2010 ◽  
Vol 73 (2) ◽  
pp. 266-273 ◽  
Author(s):  
BLEDAR BISHA ◽  
NATALIA WEINSETEL ◽  
BYRON F. BREHM-STECHER ◽  
AUBREY MENDONCA
Keyword(s):  
Antimicrobial Agents ◽  
Aqueous Media ◽  
Membrane Integrity ◽  
Viable Cell ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Live Cells ◽  
Cell Counts

Grape seed extract (GSE) is a rich source of proanthocyanidins, a class of natural antioxidants reported to have wide-ranging bioactivity as anti-inflammatory, anticarcinogenic, and antimicrobial agents. The ability of GSE to rapidly inactivate Listeria monocytogenes in vitro and the generally recognized as safe status of GSE make this extract an attractive candidate for control of Listeria in or on foods. Previously, GSE has been used at relatively high concentrations (1%) in complex food matrices and in combination with other antimicrobials. We sought to characterize the antilisterial effects of a commercial GSE preparation (Gravinol-S) alone at much lower concentrations (0.00015 to 0.125%) in aqueous solution and to test its possible use as an antimicrobial wash for fresh produce surfaces. Based on broth microdilution tests, the MICs of GSE against L. monocytogenes Scott A and Listeria innocua ATCC 33090 were as low as 50 and 78 μg ml−1, respectively. GSE was evaluated in 0.85% saline against live cells of L. innocua via flow cytometry, using propidium iodide as a probe for membrane integrity. At sub-MICs and after only 2 min of exposure, treatment with GSE caused rapid permeabilization and clumping of L. innocua, results that we confirmed for L. monocytogenes using fluorescence microscopy and Live/Dead staining. At higher concentrations (0.125%), GSE reduced viable cell counts for L. monocytogenes by approximately 2 log units within 2 min on tomato surfaces. These results suggest the potential for GSE as a natural control of Listeria spp. on low-complexity foods such as tomatoes.

Sign in / Sign up

Export Citation Format

Share Document