scholarly journals Using a surface response method for experimental design to optimize the analysis of taurine in food supplements by capillary electrophoresis

2017 ◽  
Vol 33 (4) ◽  
Author(s):  
Nguyen Manh Huy ◽  
Nguyen Thanh Dam ◽  
Duong Hong Anh ◽  
Pham Hung Viet
Keyword(s):  
Capillary Electrophoresis ◽  
Ph Value ◽  
Milk Powder ◽  
Energy Drink ◽  
Injection Time ◽  
Sample Injection ◽  
Surface Response ◽  
Relative Standard ◽  
Surface Response Method ◽  
Response Method

Abstract: This study focus on using surface response method to optimize the taurine analysis by capillary electrophoresis with contactless conductivity detector. A response surface method (RSM) coupled with a central composite model (CCD) comprising 30 experiments was developed with 4 factors including concentration of Tris buffer, pH value, separating potential and sample injection time. The best set of factor levels for taurine analysis was found as follows: 150 mmol/L of Tris concentration, pH = 8.96, separation voltage of -10 kV, the sample injection time of 60 seconds. The validation of analytical method showed the detection limit for taurine was 0.266mg/L, linearity range from 1 to 500 mg/L, relative standard deviation values ​​for peak areas and migration times were less than 3.16%, the linear correlation coefficient gained 0.999. Recovery efficiency of taurine in several food supplement matrixes such as: fresh milk, milk powder, energy drink were in range from 91.9% to 101.7%.  

scholarly journals Development of a Capillary Electrophoresis Method for the Enantioselective Estimation of Primaquine in Pharmaceutical Formulations

2008 ◽  
Vol 91 (3) ◽  
pp. 536-541 ◽  
Author(s):  
Abdalla A Elbashir ◽  
Bahruddin Saad ◽  
Abdussalam Salhin Mohamed Ali ◽  
Muhammad Idiris Saleh
Keyword(s):  
Capillary Electrophoresis ◽  
Applied Voltage ◽  
Pharmaceutical Formulations ◽  
Fused Silica ◽  
Chiral Selector ◽  
Effective Length ◽  
Racemic Mixture ◽  
Injection Time ◽  
Relative Standard ◽  
Capillary Temperature

Abstract A capillary electrophoresis (CE) method has been developed that allows the separation and estimation of primaquine enantiomers using hydroxypropyl--cyclodextrin (HP--CD) as a chiral selector. The influence of chemical and instrumental parameters on the separation, such as type and concentration of CD, buffer concentration, buffer pH, applied voltage, capillary temperature, and injection time, were investigated. Good separation of the racemic mixture of primaquine was achieved using a fused-silica capillary (52.5 cm effective length 50 m id) and a background electrolyte composed of tris-phosphate buffer solution (50 mM, pH 2.5) containing 15 mM HP--CD as a chiral selector. The recommended applied voltage, capillary temperature, and injection time were 15 kV, 25C, and 6 s, respectively. Within-day and interday reproducibility of peak area and migration time gave relative standard deviation values ranging from 1.053.30. Good recoveries (range of 96.8104.9) were obtained from the determination of placebos that were spiked with 0.251.00 mg/L primaquine. The proposed CE method was successfully applied to the assay of primaquine diphosphate in pharmaceutical formulations (tablets).

scholarly journals A novel method of reversed migration capillary electrophoresis for determination of linear alkylbenzene sulfonates

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhuo Huang ◽  
Weike Wang ◽  
Lingxian Xie ◽  
Li Lin
Keyword(s):  
Capillary Electrophoresis ◽  
High Performance ◽  
Ph Value ◽  
High Sensitivity ◽  
Chromatography Method ◽  
Linear Alkylbenzene ◽  
Relative Standard ◽  
Linear Alkylbenzene Sulfonates ◽  
Liquid Chromatography Method ◽  
And Migration

AbstractA reversed migration capillary electrophoresis (RMCE) has been developed to determine linear alkylbenzene sulfonates (LAS). The sample stacking and separation conditions have been systematically investigated and optimized under reversed separation voltage at a low pH value. The separation effect of LAS homologs has been greatly improved based on the relative motion of electrophoresis and electroosmotic flow. RMCE demonstrates a good linear range of 0.1 mg/l to 10.0 mg/l, and the detection limit of LAS homologs reaches 0.001–0.004 mg/l. The relative standard deviations (n=6) of peak area and migration time were 2.25–4.40% and 0.67–0.75%, respectively. RMCE has also been applied for LAS detection in practical wastewater. The results show RMCE exhibits easy pretreatment, fast detection, high sensitivity, good peak shapes and resolution, and less solvent consumption, compared with the established high-performance liquid chromatography method.

scholarly journals Penentuan Kondisi Lingkungan Kerja Fisik yang Optimal Menggunakan Metode Permukaan Respon

2017 ◽  
Vol 1 (1) ◽  
pp. 1 ◽  
Author(s):  
Arta Rusidarma Putra ◽  
Anggar Guritno
Keyword(s):  
Work Environment ◽  
Working Environment ◽  
Surface Response ◽  
Operator Performance ◽  
Optimal Value ◽  
Surface Response Method ◽  
Environment Factor ◽  
Work Environment Factors ◽  
Response Method ◽  
Work Environment Factor

Many consequences caused by the condition of the work environment is not good. This can be caused by noise, lighting and temperature conditions. We need to determine the optimal value of these work environment factors in order to improve operator performance. The correlation between operator performance with work environment factor such as noise, lighting and temperature are found out by develope empirical model to describe relation between operator performance with work environment factor. The model is used to optimize operator performance. This research use surface response method. The experimental design used a central composite design. The research was conducted by experiments in the Laboratory of Analysis of Work Design and Ergonomics of Islamic University of Indonesia Yogyakarta. Variables taken are noise, lighting and temperature. Range for noise is 40 dB to 75 dB, lighting 200 lux to 700 lux, and temperatures between 20ºC to 35ºC. The experiment carried out was the resistor color matching for 5 minutes according to the combination of research variables that have been determined in the surface response method. Output is the number of truths that occur. Using this optimization model, optimal working environment conditions for noise of 60.5 dB, 354.5 lux illumination and temperature of 25.8º.

scholarly journals Extraction optimization of soluble compounds of yerba maté

2002 ◽  
Vol 45 (2) ◽  
pp. 189-193 ◽  
Author(s):  
César Sambiassi ◽  
Andrea M. Escalada ◽  
Miguel E. Schmalko
Keyword(s):  
Experimental Design ◽  
Linear Equation ◽  
Water Soluble ◽  
Extraction Time ◽  
Yerba Mate ◽  
Surface Response ◽  
Extract Concentration ◽  
Extraction Optimization ◽  
Surface Response Method ◽  
Response Method

The objective of this research was the extraction optimization of water soluble compounds of yerba maté. Measures variables were extract concentration and weight of leaves and twigs. Controlled variables were time and temperature of extraction and water/solid relation. A surface response method of three variables was used as experimental design, with 20 experiences in each case. The range of each variable, defined in the experimental design, was: extraction time, 13.2 to 46.8 minutes; temperature, 48.2 to 81.8°C and water solid relation, 4.64 to 11.36 g water/100 g of dry solid. Extract weight varied from 13.14 to 29.56 g in leaves and 8.98 to 16.32 g in twigs (each one per 100 g of dry solid). Extract concentration varied between 2.17 and 3.43 g/100 ml in leaves and between 1.32 and 2.31 g/100 ml in twigs. The results were fit to a linear equation in each case.

Sign in / Sign up

Export Citation Format

Share Document