scholarly journals Isolation and Selection of Purple Non-Sulfur Bacteria for Nutrient Rich Biomass Production from Wastes

2021 ◽  
Vol 37 (2) ◽  
Author(s):  
Do Binh Minh ◽  
Pham The Hai
Keyword(s):  
Biomass Production ◽  
Morphological Characteristics ◽  
Nitrogen Sources ◽  
Pond Water ◽  
The Other ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Sulfur Bacteria ◽  
Potential Applications ◽  
Excessive Nutrients

Purple nonsulfur bacteria (PNSB) are anoxygenic photosynthetic bacteria, which are able to photoheterotrophically grow in the presence of excessive nutrients. Hence, PNSB can convert organic components, in waste waters into nutrient rich biomass. This suggests the feasibility of converting wastes into valuable products. In this research, method for isolation of PNSB was optimized, and subsequently used for the isolation of  PNSB from domestic waste and pond water samples taken in Hanoi. In addition, the effects of carbon and nitrogen sources and types of waste on biomass and nutrients (protein and carotenes) were also investigated. Four PNSB strains were isolated and based on comparative 16S rDNA analysis and their morphological characteristics, they were identified as Rhodobacter (Rb.) capsulatus, Rhodobacter (Rb.) sphaeroides, Rhodobacter (Rb.) sediminis and Rhodopseudomonas (Rp.) palustris. In terms of the effects of carbon and nitrogen sources on their biomass and nutrient production, glucose or maltose and ammonium chloride or urea were found to be more enhancive than starch and peptone, respectively. Among the studied strains, Rb. capsulatus MD1 and Rb. sphaeroides MD3 showed significantly higher biomass production (up to 0.7 g/l) when growing with various carbon and nitrogen sources, in comparison with the other strains. Moreover,  MD1 and MD3 also produced at least four-fold more carotenoid and up to two-fold more protein in tofu processing wastewater compared with the other wastewater. The results suggest potential applications of the PNSB strains for efficient conversions of organic compounds in wastes into biomass of high nutritional values.  

scholarly journals Production of Phytase by three thermophilic fungi

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Ranjith Kumar R ◽  
Rajendar Reddy ◽  
Girisham S ◽  
Reddy S.M.
Keyword(s):  
Carbon Source ◽  
Nitrogen Sources ◽  
The Other ◽  
Thermomyces Lanuginosus ◽  
Thermophilic Fungi ◽  
Carbon And Nitrogen Sources ◽  
Phytase Production ◽  
Carbon And Nitrogen ◽  
Cultural Conditions ◽  
Other Hand

Production of phytase by three thermophilic fungi, Thermomyces lanuginosus, Talaromyces luteus and Rhizomucor pusillus under different cultural conditions was assessed. Temperature of 45°C, pH-6.0 were optimum for phytase production by the all three fungi under investigation . Carbon and nitrogen sources for production of phytases by the three thermophilic fungi varied with the fungus. When T. lanuginosus opted for D-glucose followed by D-fructose, T. luteus preferred D-glucose, D-mannose and mannitol for production a phytase. On the other hand, R. pusillus produced maximum phytase during its growth on mannitol and maltose as carbon source. L- asparagine, L- arginine and L-asparatic acid were preferred nitrogen sources for production of phytase by T. lanuginosus. On the other hand T. luteus, opted for L- asparagine, L-glutamic acid and L- glycine for the activity of phytase. R. pusillus produced maximum phytase in medium containing L-argine, L-asparagine and L- asparatic acid.

The Screening of Optimal Ganoderma lucidum and the Effect of Medium Substrate on Bioactive Metabolite in Submerged Culture

2012 ◽  
Vol 554-556 ◽  
pp. 887-890
Author(s):  
Mei Lin Cui ◽  
Guo Qing He
Keyword(s):  
Yeast Extract ◽  
Ganoderma Lucidum ◽  
Submerged Culture ◽  
Nitrogen Sources ◽  
The Other ◽  
Carbon And Nitrogen Sources ◽  
Bioactive Metabolite ◽  
Carbon And Nitrogen ◽  
Other Hand

To determine an optimal G. lucidum strain, we made the production of mycelia biomass, EPS, IPS, IT, ET as indicators, among the five tested strains, the American G. lucidum was screened, and the production of mycelia biomass, EPS, IPS, IT, ET could reach 2.013g/100 ml, 11.2988 mg/100ml, 23.7800 mg/10ml, 45.5412 mg/100ml, 11.1417 mg/100ml, respectively. On the other hand, according to the screening of carbon and nitrogen sources as well as their concentration, the suitable carbon and nitrogen was 3% malt powder, 1.5% yeast extract. So we can use the fermentation culture for the following research, which was as follows：3% malt powder, 1.5%yeast extract, 0.3% KH2PO4, 0.15% MgSO4, 0.005% VB1.

Biodegradation of cyanide compounds by a Pseudomonas species (S1)

1999 ◽  
Vol 45 (3) ◽  
pp. 201-208 ◽  
Author(s):  
J K Dhillon ◽  
N Shivaraman
Keyword(s):  
Formic Acid ◽  
Binary Mixtures ◽  
Nitrogen Sources ◽  
Transformation Products ◽  
The Other ◽  
Pseudomonas Sp ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Enrichment Technique ◽  
Pseudomonas Species

A Pseudomonas sp. (S1), isolated from soil by an enrichment technique was tested for its potential to degrade different cyanide compounds. Further, biodegradation/biotransformation of binary mixtures of the cyanide compounds by the culture was also studied. The results indicated that the culture could grow on the following nitriles by using them as carbon and nitrogen sources: acetonitrile, butyronitrile, acrylonitrile, adiponitrile, benzonitrile, glutaronitrile, phenylacetonitrile, and succinonitrile. Studies on the biodegradation of these cyanide compounds in binary mixtures showed that the presence of acrylonitrile or KCN delayed the degradation of acetonitrile in a mixture, while none of the other cyanide compounds affected the degradation of one another. The transformation products of the nitriles were their corresponding acids, and similarly, KCN was also directly transformed to formic acid. Studies on the transformation of these cyanide compounds showed that the rate of transformation of nitriles to their corresponding carboxylic acids was acrylonitrile > acetonitrile > adiponitrile > benzonitrile > KCN. This culture has the unique characteristic of transforming representatives of saturated aliphatic, aliphatic olefinic, aromatic, and aralkyl nitriles, as well as alkali cyanide, to their corresponding carboxylic acids.Key words: Pseudomonas sp.(S1), biodegradation, biotransformation, nitriles, cyanide.

scholarly journals Sıvı Kültürde Lepista Nuda Tarafından Miselyal Biyokütle ve Ekzopolisakkarit Üretimi İçin Karbon ve Azot Kaynaklarının Belirlenmesi

2018 ◽  
Vol 6 (5) ◽  
pp. 581
Author(s):  
Murat Özdal
Keyword(s):  
Biomass Production ◽  
Submerged Culture ◽  
Nitrogen Sources ◽  
Edible Mushroom ◽  
Mycelial Biomass ◽  
Carbon And Nitrogen Sources ◽  
Medium Carbon ◽  
Carbon And Nitrogen ◽  
Initial Medium ◽  
Optimized Conditions

In the present study, Lepista nuda, an edible mushroom, was used for the production of exopolysaccharide in the submerged culture. The effects of initial medium carbon and nitrogen sources on exopolysaccharide and mycelial biomass production by L. nuda IT strain were investigated. The highest exopolysaccharide production was obtained when culture parameters were used as initial medium 50 g/L glucose and 5 g/L yeast extract. As a result, 2.12 g/L exopolysaccharide and 12.3 g/L biomass production in optimized conditions at flask scales were achieved. This is the first study showing the production of exopolysaccharide in liquid culture of Lepista nuda.

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production

Growth Conditions of a Thermophilic Geobacillus sp. as an Enhanced Oil Recovery Material

2012 ◽  
Vol 496 ◽  
pp. 457-460
Author(s):  
Xiang Ping Kong
Keyword(s):  
Enhanced Oil Recovery ◽  
Bacterial Growth ◽  
Oil Recovery ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Good Growth ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

The growth conditions of a Geobacillus sp. were investigated by single-factor experiments. The strain was strictly aerobic bacterium, and could grow on hydrocarbons as the sole carbon source. The optimum carbon and nitrogen sources were 3.0% sucrose and 0.20% KNO3, respectively. The range of temperature, salinity and pH for the bacterial growth was 35-70 °C, 0-10% NaCl and 5.5-9.5, and good growth was obtained at 35-65 °C, 0.5-8% NaCl and 6.0-9.0, respectively. Particularly, the optimum temperature for the bacterial growth was between 50 °C and 60 °C. The strain had wide adaptability to the extreme conditions, and may be potentially applied to microbial enhanced oil recovery and oil-waste bioremediation technology.

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