scholarly journals Identifikasi Bakteri Infeksi Saluran Pernafasan Bawah Non Tuberkulosis (Non TB) dan Pola Resistensinya pada Penderita Diabetes Melitus di RSUP M. Djamil

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Virgi Anggia Lubis ◽  
Yusticia Katar ◽  
Elizabeth Bahar
Keyword(s):  
Diabetes Mellitus ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Respiratory Infection ◽  
Bacterial Resistance ◽  
Klebsiella Pneumonia ◽  
Lower Respiratory Infection ◽  
Streptococcus Pneumonia ◽  
Medicine Department ◽  
Patients With Diabetes

AbstrakKadar gula darah yang tinggi pada pasien diabetes mellitus (DM) menyebabkan pasien ini rentan akan terjadinya infeksi, salah satunya infeksi saluran pernafasan bawah non tuberkulosis (Non TB) yang disebabkan oleh bakteri Gram negatif dan Gram positif, maka terapi pilihannya antibiotik spektrum luas. Survei awal di Bagian Penyakit Dalam RSUP M. Djamil didapatkan bahwa terapi yang dilakukan adalah terapi empiris yang  mengakibatkan meningkatnya resistensi. Tujuan penelitian ini adalah mengidentifikasi bakteri penyebab infeksi pernafasan bawah non tuberkulosis dan pola resistensinya pada penderita DM di  RSUP M. Djamil. Penelitian deskriptif ini dilakukan di Laboratorium Mikrobiologi FK Unand Padang dan Bagian Penyakit Dalam RSUP M. Djamil terhadap 16 pasien dengan diagnosis DM disertai infeksi saluran pernafasan bawah dari Januari sampai Februari 2014. Hasil penelitian menunjukkan Klebsiella pneumonia (56,25%) sebagai penyebab terbanyak, diikuti Staphylococcus aureus (18,25%), Pseudomonas aeruginosa (12,50%), dan Streptococcus pneumonia (12.50%). Uji resistensi menunjukkan Klebsiella pneumonia mengalami resistensi yang besar terhadap Ceftriaxone (66,63%), Pseudomonas aeruginosa resisten terhadap Ceftriaxone dan Amoxicilin Clavulanat Acid (50%), Staphylococcus aureus resisten terhadap Ciprofloxacin (33,33), sedangkan Streptococcus pneumonia  sensitif terhadap Azitromicin (100%). Dapat disimpulkan bakteri yang ditemukan mengalami resistensi yang cukup besar terhadap beberapa antibiotik yang digunakan.Kata kunci: diabetes melitus, infeksi saluran pernafasan bawah no TB, bakteri, resistensi AbstractHigh concentration of blood glucose in patients with diabetes mellitus cause susceptible to be infected, including lower respiratory infections non tuberculosis caused by Gram negatif and Gram positif. Treatment of these infections are broad spectrum antibiotics. The objective of this study was to indentify the causal bacteria of lower respiratory infection non tuberculous infection and the  bacterial resistance patterns in patients with diabetes mellitus in RSUP M. Djamil. From the primary survey in RSUP M. Djamil Internal Medicine Department, the treatment that usually used is empirical therapy that could increase risk of bacterial resistance. This descriptive study was conducted in Microbiology laboratory Medical Faculty of Andalas University and Inpatient Care of Internal Medicine Department of RSUP M. Djamil to 16 patients with Diabetes Mellitus and lower respiratory infection from January until February 2014. Culture result showed that Klebsiella pneumonia (56,25%)  was the main cause, followed by Staphylococcus aureus (18,25%), Pseudomonas aeruginosa (12,50%), and Streptococcus pneumonia(12,50%). Sensitivity test result shows that Klebsiella pneumonia has great resistance to Ceftriaxone (66,63%), Pseudomonas aeruginosa is resistant to Ceftriaxone and Amoxicilin Clavulanat Acid (50%), Staphylococcus aureus is resistant to Ciprofloxacin (33,33), while Streptococcus pneumonia is sensitive to Azitromicin (100%). It can be concluded that the bacteria found had a appreciable resistance to some antibiotics used.Keywords: diabetes mellitus, lower respiratory infection non TB, bacteria, resistance

scholarly journals Microbiological Study of Diabetic Foot Ulcer

2019 ◽  
Vol 10 (1) ◽  
pp. 50-55
Author(s):  
Mohammuddunnobi ◽  
Tasnuva Jahan ◽  
Abdullah Al Amin
Keyword(s):  
Diabetes Mellitus ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Proteus Mirabilis ◽  
Diabetic Foot ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Klebsiella Pneumonia ◽  
Coagulase Negative Staphylococci

Background: Diabetic foot is one of the most feared complications of diabetes and is the leading cause of hospitalization in diabetic patients. Limb-threatening diabetic infections are usually polymicrobial involving multiple aerobic and anaerobic organisms. Methodology: The present study was a cross sectional study, conducted in the department of surgery and microbiology at BIRDEM General Hospital, Dhaka, over a period of 9 months during January 2017- September' 2017. The study included a total of 77 adult patients of clinically diagnosed diabetic foot patients presenting to outpatient department and emergency ward. The standard case definition of diabetic foot is 'any pathology occurring in the foot of a patient suffering from diabetes mellitus or as a result of long term complication of diabetes mellitus'. Results: Majority 17(22.1%) patients had Klebsiella pneumonia, 14(18.2%) had Pseudomonas aeruginosa, 11(14.3%) had Staphylococcus aureus, 10(13.0%) had Escherichia coli, 6(7.8%) had Coagulase-negative staphylococci and 8(10.4%) had Providencia spp. In Escherichia coli 100% sensitivity to imipenem, 70% to amoxicillin-clavulanic acid, amikacin, piperacillin-tazobactam. In Coagulasenegative Staphylococci 83.3% sensitivity to tetracycline, 66.7% to ceftriaxone. In Proteus mirabilis 100% sensitivity to tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam. In Enterococcus spp.75.0% sensitivity to tetracycline. In Citrobacter spp. 100% sensitivity to imipenem. Conclusion: Common organism found in diabetic foot ulcer patients were Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Coagulase-negative staphylococci and Providencia spp. In tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam was 100% sensitive in Proteus mirabilis and only imipenem found in Escherichia coli and Citrobacter spp. Anwer Khan Modern Medical College Journal Vol. 10, No. 1: Jan 2019, P 50-55

scholarly journals Pola Kuman dan Uji Kepekaan pada Pasien Community Acquired Pneumonia di RSUP Dr. M. Djamil Padang Tahun 2016

2019 ◽  
Vol 8 (3) ◽  
pp. 553
Author(s):  
Narlis Narlis ◽  
Ellyza Nasrul ◽  
Efrida Efrida
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Community Acquired Pneumonia ◽  
Klebsiella Pneumonia ◽  
Proteus Vulgaris ◽  
Streptococcus Pneumonia

Community acquired pneumonia (CAP) merupakan infeksi yang paling sering menyebabkan sepsis dan dapat menimbulkan kematian. Pertimbangan pemilihan antimikroba yang tepat dan menghindari penggunaan yang berlebihan perlu dilakukan untuk mencegah terjadinya resistensi. Tujuan penelitian ini adalah mengetahui pola kuman dan uji kepekaan pasien CAP di RSUP Dr. M. Djamil Padang. Penelitian ini merupakan penelitian deskriptif retrospektif terhadap 201 sampel sputum pasien CAP yang diperiksa kultur kemudian dilanjutkan dengan pewarnaan Gram dan uji biokimia untuk mengidentifikasi bakteri. Uji kepekaan antimikroba menggunakan metode difusi cakram. Penelitian dilakukan di Laboratorium Sentral RSUP Dr. M. Djamil Padang periode Januari 2016 hingga Desember 2016. Data ditampilkan dalam bentuk tabel distribusi frekuensi. Mikroorganisme yang ditemukan adalah Klebsiella pneumonia (55,23%), Staphylococcus aureus (25,87%), Streptococcus pneumonia (7,96%), Pseudomonas aeruginosa (5,97%), Staphylococcus epidermidis (2,9%), Acinetobacter baumani (1,99%), Proteus vulgaris (0,49%). Antimikroba yang paling sensitif adalah Meropenem (78,7%), sedangkan antimikroba resisten dengan persentase tertinggi adalah Ampisilin (90%), Amoksisilin (89,4%), Eritromisin (73,5%), Amoksisilin + Klavulanat (64,6%), dan Kloramfenikol (61,7%). Mikroorganisme yang paling banyak ditemukan adalah Klebsiella pneumonia (55,23%). Meropenem merupakan antimikroba sensitif dengan persentase paling tinggi (78,7%) sedangkan antimikroba resisten dengan persentase paling tinggi adalah ampisilin (90%).

scholarly journals Microbiological Study of Diabetic Foot Ulcer

2018 ◽  
Vol 8 (3) ◽  
pp. 251-256
Author(s):  
Mohammuddunnobi ◽  
Tasnuva Jahan ◽  
Abdullah Al Amin
Keyword(s):  
Diabetes Mellitus ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Diabetic Foot ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Klebsiella Pneumonia ◽  
Diabetic Patients ◽  
Coagulase Negative Staphylococci

Background: Diabetic foot is one of the most feared complications of diabetes and is the leading cause of hospitalization in diabetic patients. Limb-threatening infection in diabetic patients are usually polymicrobial involving both multiple aerobic and anaerobic organisms.Methods: The present study was a cross sectional study, conducted in the Department of Surgery and Microbiology at BIRDEM General Hospital, Dhaka, over a period of 9 months during January 2017- September’ 2017. The study included a total of 77 adult patients of clinically diagnosed diabetic foot patients presenting to outpatient department and emergency ward. The standard case definition of diabetic foot is ‘any pathology occurring in the foot of a patient suffering from diabetes mellitus or as a result of long term complication of diabetes mellitus’.Results: 17(22.1%) patients had Klebsiella pneumonia, 14(18.2%) had Pseudomonas aeruginosa, 11(14.3%) had Staphylococcus aureus, 10(13.0%) had Escherichia coli, 6(7.8%) had Coagulase-negative staphylococci and 8(10.4%) had Providencia spp. In Escherichia coli 100% sensitivity to imipenem, 70% to amoxicillinclavulanic acid, amikacin, piperacillin-tazobactam. In Coagulase-negative Staphylococci 83.3% sensitivity to tetracycline, 66.7% to ceftriaxone. In Proteus mirabilis 100% sensitivity to tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam. In Enterococcus spp.75.0% sensitivity to tetracycline. In Citrobacter spp. 100% sensitivity to imipenem.Conclusion: Common organism found in diabetic foot ulcer patients were Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Coagulase-negative staphylococci and Providencia spp. In tetracycline, amikacin, ceftriaxone, imipenem, piperacillin-tazobactam was 100% sensitive in Proteus mirabilis and only imipenem found in Citrobacter spp.Birdem Med J 2018; 8(3): 251-256

scholarly journals Pola Kuman dan Uji Kepekaan pada Pasien Community Acquired Pneumonia di RSUP Dr. M. Djamil Padang Tahun 2016

2019 ◽  
Vol 8 (3) ◽  
pp. 553
Author(s):  
Narlis Narlis ◽  
Ellyza Nasrul ◽  
Efrida Efrida
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Community Acquired Pneumonia ◽  
Klebsiella Pneumonia ◽  
Proteus Vulgaris ◽  
Streptococcus Pneumonia

Community acquired pneumonia (CAP) merupakan infeksi yang paling sering menyebabkan sepsis dan dapat menimbulkan kematian. Pertimbangan pemilihan antimikroba yang tepat dan menghindari penggunaan yang berlebihan perlu dilakukan untuk mencegah terjadinya resistensi. Tujuan penelitian ini adalah mengetahui pola kuman dan uji kepekaan pasien CAP di RSUP Dr. M. Djamil Padang. Penelitian ini merupakan penelitian deskriptif retrospektif terhadap 201 sampel sputum pasien CAP yang diperiksa kultur kemudian dilanjutkan dengan pewarnaan Gram dan uji biokimia untuk mengidentifikasi bakteri. Uji kepekaan antimikroba menggunakan metode difusi cakram. Penelitian dilakukan di Laboratorium Sentral RSUP Dr. M. Djamil Padang periode Januari 2016 hingga Desember 2016. Data ditampilkan dalam bentuk tabel distribusi frekuensi. Mikroorganisme yang ditemukan adalah Klebsiella pneumonia (55,23%), Staphylococcus aureus (25,87%), Streptococcus pneumonia (7,96%), Pseudomonas aeruginosa (5,97%), Staphylococcus epidermidis (2,9%), Acinetobacter baumani (1,99%), Proteus vulgaris (0,49%). Antimikroba yang paling sensitif adalah Meropenem (78,7%), sedangkan antimikroba resisten dengan persentase tertinggi adalah Ampisilin (90%), Amoksisilin (89,4%), Eritromisin (73,5%), Amoksisilin + Klavulanat (64,6%), dan Kloramfenikol (61,7%). Mikroorganisme yang paling banyak ditemukan adalah Klebsiella pneumonia (55,23%). Meropenem merupakan antimikroba sensitif dengan persentase paling tinggi (78,7%) sedangkan antimikroba resisten dengan persentase paling tinggi adalah ampisilin (90%).

scholarly journals A Comparison of Pathogens in Skin and Soft Tissue Infections and Pedal Osteomyelitis in Puncture Wound Injuries Affecting the Foot

2020 ◽  
Author(s):  
David H Truong ◽  
Javier La Fontaine ◽  
Matthew Malone ◽  
Dane K Wukich ◽  
Kathryn E Davis ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Soft Tissue ◽  
Soft Tissue Infection ◽  
Bacterial Pathogen ◽  
Soft Tissue Infections ◽  
Puncture Wound ◽  
Common Pathogen ◽  
Patients With Diabetes

ObjectiveTo compare pathogens involved in skin and soft tissue infection (SSTI) and pedal osteomyelitis (OM) in patients with and without diabetes with puncture wounds to the foot. MethodsWe evaluated 113 consecutive patients between June 2011 and March 2019 with foot infection (SSTI and OM) from a puncture injury sustained to the foot. Eighty-three patients had diabetes (DM) and 30 did not (NDM). We evaluated the bacterial pathogens in patients with skin and soft tissue infections (SSTI) and pedal osteomyelitis (OM). ResultsPolymicrobial infection were more common in patients with diabetes mellitus (83.1% vs 53.3%, p=.001). The most common pathogen for SSTI and OM in DM was s. aureus (SSTI 50.7%, OM 32.3%), whereas in NDM patients it was Pseudomonas (25%) for SSTI. Anaerobes (9.4%) and fungal (3.1%) infection were uncommon. Pseudomonas aeruginosa was only identified in 5.8% of people with diabetes. ConclusionsThe most common bacterial pathogen in both SSTIs and pedal OM was staphylococcus aureus in patients with DM. Pseudomonas spp., was the most common pathogen in people without diabetes with SSTIs.

scholarly journals Evaluation of Moringa Peregrina (Forsk) Fiori, Leaf and Seed Extract Against Multidrug Resistant Strains of Bacteria and Fungus of Clinical Origin

2021 ◽  
Vol 3 (1) ◽  
pp. 20-26
Author(s):  
Suliman Mansour Albalawi ◽  
Abdulrahman K. Al-Asmari ◽  
Syed Rafatullah ◽  
Maysa Mahfoud
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Growth Inhibition ◽  
Acinetobacter Baumannii ◽  
Bacterial Resistance ◽  
Antimicrobial Agents ◽  
Colorimetric Assay ◽  
Multidrug Resistant ◽  
Klebsiella Pneumonia ◽  
Isolation And Purification ◽  
Moringa Peregrina

  The emergence of antibiotic resistant microorganism strains has become a critical concern in the treatment of infectious diseases and makes the search of an alternative therapy a must. The study was designed to evaluate the in vitro antimicrobial activities of the Moringa peregrina (MP) leave (MPL) and seed (MPS) extracts. Antimicrobial assays were performed using a microplate growth inhibition assay against 11 multidrug-resistant (MDR) strains. Following qualitative analysis, dose-response assays were performed using the MTT colorimetric assay. The results showed a strong correlation between the MPL and MPS extract concentration and growth inhibition (P<0.001). MP extract revealed a remarkable antimicrobial effect and inhibited the growth and survival of MDR pathogens which include Escherichia coli; Pseudomonas aeruginosa; Klebsiella pneumonia; Acinetobacter baumannii; Staphylococcus aureus between (88.6-94.7 %) and between (62.3- 88.7%) against Candida Kefyer; Candida parapsilosis; Candida albicans; Candida glabrata; Aspergillus flavus and Fusarium oxysporum. MIC50 ranging from ≤6.25 to 25 mg/mL. Acinetobacter baumannii and Pseudomonas aeruginosa were the most susceptible to MP extracts (MIC50 < 6.25 mg/mL). These results support the use of MP in Arab traditional medicine as natural antimicrobial agents. Additionally, the use of such naturally occurring adjuvant derived from medicinal plants can be used as an adjuvant with synthetic antibiotics to combat bacterial resistance and to enhance the antibacterial potential. Further studies are recommended on isolation and purification of novel antimicrobial molecules to treat the infections caused by microbes.  

scholarly journals Evaluation of Short-Term Exposure to 2.4 GHz Radiofrequency Radiation Emitted from Wi-Fi Routers on the Antimicrobial Susceptibility of Pseudomonas aeruginosa and Staphylococcus aureus

2020 ◽  
Vol 9 ◽  
pp. 1580 ◽  
Author(s):  
Samad Amani ◽  
Mohammad Taheri ◽  
Mohammad Mehdi Movahedi ◽  
Mohammad Mohebi ◽  
Fatemeh Nouri ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Susceptibility ◽  
Electromagnetic Waves ◽  
Bacterial Resistance ◽  
Diffusion Method ◽  
Short Term ◽  
Radiofrequency Radiation ◽  
Short Term Exposure ◽  
Antibiotic Susceptibility Test

Background: Overuse of antibiotics is a cause of bacterial resistance. It is known that electromagnetic waves emitted from electrical devices can cause changes in biological systems. This study aimed at evaluating the effects of short-term exposure to electromagnetic fields emitted from common Wi-Fi routers on changes in antibiotic sensitivity to opportunistic pathogenic bacteria. Materials and Methods: Standard strains of bacteria were prepared in this study. Antibiotic susceptibility test, based on the Kirby-Bauer disk diffusion method, was carried out in Mueller-Hinton agar plates. Two different antibiotic susceptibility tests for Staphylococcus aureus and Pseudomonas aeruginosa were conducted after exposure to 2.4-GHz radiofrequency radiation. The control group was not exposed to radiation. Results: Our findings revealed that by increasing the duration of exposure to electromagnetic waves at a frequency of 2.4 GHz, bacterial resistance increased against S. aureus and P. aeruginosa, especially after 24 hours (P<0.05). Conclusion: The use of electromagnetic waves with a frequency of 2.4 GHz can be a suitable method for infection control and treatment. [GMJ.2020;9:e1580]

scholarly journals The Study of Antibacterial Properties of Anbarnasara Smoke on Multi-Drug Resistant Bacteria Isolated From Urinary Infection in Pregnant Women

2020 ◽  
Vol 8 (4) ◽  
pp. 110-115
Author(s):  
Afsaneh Molamirzaei ◽  
Maryam Allahdadian ◽  
Monir Doudi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Pregnant Women ◽  
Urinary Infection ◽  
Antibacterial Properties ◽  
Resistant Bacteria ◽  
Klebsiella Pneumonia ◽  
Drug Resistant

Background: Using smoke from burning donkey dung has been popular in the treatment of many diseases in Iran. Objective: This study aimed to investigating the antimicrobial properties of donkey dung smoke on multi-drug resistant (MDR) bacteria isolated from urinary infection. Materials and Methods: First, 300 and 200 urine samples were collected from pregnant and non-pregnant women in Isfahan, Iran. Then in each group, 100 bacterial isolates including Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Staphylococcus saprophyticus were isolated. Antibiotic resistant protocol was determined by antibiogram test. Donkey dung was sterilized, disintegrated, and heated. The smokes were concentrated in n-hexane solvent (65%) and were collected after evaporation of the solvent. Finally, the antibacterial activities of the concentrations of 0.25, 0.5 and 1 mg/mL of the smokes were detected using disk diffusion and macrodilution methods. Results: The most abundant MDR isolates causing urinary infections in pregnant and non-pregnant women was Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of donkey dung smoke on MDR isolates from pregnant women were 0.25 mg/mL and 0.5 mg/mL, respectively. In the case of MDR isolates in non-pregnant women, the MIC of the smoke on Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was 0.25 mg/mL, and the MBC on these isolates was 0.5 mg/mL. Conclusion: The smokes from donkey dung investigated in the present study have suitable potentials for controlling the infections after In vivo analysis.

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