Dwindling status of Epimedium Elatum (Morren & Decne) and its geographical distribution in Kashmir Himalaya, India

2018 ◽  
pp. 47-52
Keyword(s):  
Medicinal Plant ◽  
Biological Activities ◽  
Conservation Status ◽  
Natural Populations ◽  
Culture Techniques ◽  
Kashmir Himalayas ◽  
Near Future ◽  
First Time ◽  
Tissue Culture Techniques

Epimedium elatum (Morren & Decne) of family Berberidaceace is a rare perennial medicinal plant, endemic to high altitude forests of Northwestern Himalayas in India. Ethnobotanically, it has been used as an ingredient for treatment of bone-joint disorders, impotence and kidney disorders in Kashmir Himalayas. Phytochemically, it is rich in Epimedin ABC and Icariin; all of these have been demonstrated to possess remarkable biological activities like PDE-5 inhibition (treatment of erectile dysfunction), anticancer, antiosteoporosis antioxidant and antiviral properties. The present investigation reports its traditional usage, comprehensive distribution and conservation status from twenty ecogeographical regions in Kashmir Himalayas, India. The species was reported from Gurez valley for the first time. Numerous threats like excessive grazing, deforestration, habitat fragmentation, tourism encroachment, landslides and excessive exploitation have decreased its natural populations in most of the surveyed habitats. Consequently, its existence may become threatened in near future if timely conservation steps are not taken immediately by concerned stakeholders involved in medicinal plant research. Moreover, use of plant tissue culture techniques is recommended for development of its in vitro propagation protocols. Therefore, introduction of this medicinal plant in botanical gardens, protected sites and development of monitoring programmes are needed for its immediate conservation in Northwestern Himalayas, India.

scholarly journals Dwindling status of Epimedium elatum (Morren & Decne) and its geographical distribution in Kashmir Himalaya, India

2018 ◽  
pp. 47-52
Author(s):  
Sajad A. Lone, Saleem Mushtaq, Qazi P. Hassan ◽  
Suphla Gupta
Keyword(s):  
Medicinal Plant ◽  
Biological Activities ◽  
Conservation Status ◽  
Natural Populations ◽  
Culture Techniques ◽  
Kashmir Himalayas ◽  
Near Future ◽  
First Time ◽  
Tissue Culture Techniques

Epimedium elatum (Morren & Decne) of family Berberidaceace is a rare perennial medicinal plant, endemic to high altitude forests of Northwestern Himalayas in India. Ethnobotanically, it has been used as an ingredient for treatment of bone-joint disorders, impotence and kidney disorders in Kashmir Himalayas. Phytochemically, it is rich in Epimedin ABC and Icariin; all of these have been demonstrated to possess remarkable biological activities like PDE-5 inhibition (treatment of erectile dysfunction), anticancer, antiosteoporosis antioxidant and antiviral properties. The present investigation reports its traditional usage, comprehensive distribution and conservation status from twenty ecogeographical regions in Kashmir Himalayas, India. The species was reported from Gurez valley for the first time. Numerous threats like excessive grazing, deforestration, habitat fragmentation, tourism encroachment, landslides and excessive exploitation have decreased its natural populations in most of the surveyed habitats. Consequently, its existence may become threatened in near future if timely conservation steps are not taken immediately by concerned stakeholders involved in medicinal plant research. Moreover, use of plant tissue culture techniques is recommended for development of its in vitro propagation protocols. Therefore, introduction of this medicinal plant in botanical gardens, protected sites and development of monitoring programmes are needed for its immediate conservation in Northwestern Himalayas, India.

Potential of Tissue Cultured Medicinal Plants in Malaysia

2013 ◽  
Vol 62 (1) ◽  
Author(s):  
Zahidah Ayob ◽  
Alina Wagiran ◽  
Azman Abd Samad
Keyword(s):  
Medicinal Plants ◽  
Biological Activities ◽  
Centella Asiatica ◽  
Zingiber Officinale ◽  
Secondary Products ◽  
Culture Techniques ◽  
Kaempferia Galanga ◽  
Zingiber Officinale Roscoe ◽  
Tissue Culture Techniques

Medicinal plants possess many secondary products that exhibit biological activities such as antioxidant, anticancer, anti-inflammatory, antibacterial and anti microbial. Scientific findings have demonstrated that tissue culture techniques could be an alternative tool to propagate plant in vitro and manipulate secondary metabolites in medicinal plants. This review aims to give an update on the various plant regeneration of some locally used medicinal plants in Malaysia such as Eurycome longifolia Jack, Zingiber officinale Roscoe, Centella asiatica L., Justicia gendarussa Burm. f, Kaempferia galanga L. and Orthosiphon stamineus Benth. Different type of cultures including organ, callus and cell cultures is also discussed.

Tamarix articulata (T. articulata) - An Important Halophytic Medicinal Plant with Potential Pharmacological Properties

2019 ◽  
Vol 20 (4) ◽  
pp. 285-292 ◽  
Author(s):  
Abdullah M. Alnuqaydan ◽  
Bilal Rah
Keyword(s):  
Medicinal Plant ◽  
Biological Activities ◽  
Wide Spectrum ◽  
Biological Properties ◽  
In Vivo Model ◽  
Drug Candidate ◽  
Phytochemical Analysis ◽  
Pharmacological Properties

Background:Tamarix Articulata (T. articulata), commonly known as Tamarisk or Athal in Arabic region, belongs to the Tamaricaece species. It is an important halophytic medicinal plant and a good source of polyphenolic phytochemical(s). In traditional medicines, T. articulata extract is commonly used, either singly or in combination with other plant extracts against different ailments since ancient times.Methods:Electronic database survey via Pubmed, Google Scholar, Researchgate, Scopus and Science Direct were used to review the scientific inputs until October 2018, by searching appropriate keywords. Literature related to pharmacological activities of T. articulata, Tamarix species, phytochemical analysis of T. articulata, biological activities of T. articulata extracts. All of these terms were used to search the scientific literature associated with T. articulata; the dosage of extract, route of administration, extract type, and in-vitro and in-vivo model.Results:Numerous reports revealed that T. articulata contains a wide spectrum of phytochemical(s), which enables it to have a wide window of biological properties. Owing to the presence of high content of phytochemical compounds like polyphenolics and flavonoids, T. articulata is a potential source of antioxidant, anti-inflammatory and antiproliferative properties. In view of these pharmacological properties, T. articulata could be a potential drug candidate to treat various clinical conditions including cancer in the near future.Conclusion:In this review, the spectrum of phytochemical(s) has been summarized for their pharmacological properties and the mechanisms of action, and the possible potential therapeutic applications of this plant against various diseases discussed.

DEVELOPMENT OF NEOLAMARCKIA CADAMBA (KELEMPAYAN) TISSUE CULTURE TECHNIQUES FOR SUSTAINABLE SUPPLY OF PLANTING MATERIALS FOR COMMERCIAL PLANTATION

2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Siti Suhaila A. Rahman ◽  
Norwati Muhammad ◽  
Nor Hasnida Hassan ◽  
Haliza Ismail ◽  
Nazirah Abdullah ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Mass Production ◽  
Nodal Segments ◽  
Direct Organogenesis ◽  
Timber Species ◽  
Culture Techniques ◽  
Commercial Plantation ◽  
Planting Materials ◽  
Tissue Culture Techniques

Neolamarckia cadamba (kelempayan) is a multipurpose and fast growing timber species. The tree is grown for timber, paper-making and as ornamental plant. It is reported that its barks and leaves possesed medicinal values and its flowers are used in perfumes. The species is also known to be suitable for plywood, packing case, toys and short-fibred pulp. Therefore, mass production of high quality planting material of N. cadamba is important to support plantation program of this species. Here we presented mass production of N. cadamba through tissue culture techniques. Nodal segments derived from in vitro germinated seeds were used and induced direct organogenesis to produce shoots and roots using MS media (1962) and plant growth regulators (BAP and IBA) that are relatively cheaper than previously used methods. The tissue culture technique of N. cadamba developed may help in ensuring supply of planting materials that are feasible for commercial plantation purposes.

scholarly journals Cecropia pachystachya Leaves Present Potential to Be Used as New Ingredient for Antiaging Dermocosmetics

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Maria Fernanda Fernandes ◽  
Jessica Leiras Mota Conegundes ◽  
Nícolas de Castro Campos Pinto ◽  
Luiz Gustavo de Oliveira ◽  
Jair Adriano Kopke de Aguiar ◽  
...  
Keyword(s):  
Biological Activities ◽  
Thiobarbituric Acid ◽  
Skin Aging ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Hek 293 ◽  
Human Embryonic Kidney Cells ◽  
Glycation End Products ◽  
First Time

Several biological activities have been reported for leaf extracts of Cecropia pachystachya species, including antioxidant and wound healing activities. This study aims to report, for the first time, the antiaging potential of the hydroethanolic (HE) and the ethanolic (EE) extracts obtained from the leaves of C. pachystachya using different in vitro assays. Both HE and EE presented relevant antioxidant capacity in different models, including phosphomolybdenum, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), carotene/linoleic acid bleaching, and thiobarbituric acid reactive substances (TBARS) assays. Their ability to prevent the production of advanced glycation end products (AGEs) was also evaluated, and both extracts showed important activity, especially HE. The extracts also stimulated the fibroblasts proliferation in vitro, specialized cells that produce several mediators which maintain the skin integrity and youthfulness. Cytotoxicity of the extracts was not observed for this lineage or HEK-293, human embryonic kidney cells widely used to evaluate cytotoxicity of chemical compounds. HE also exhibited the ability to inhibit the collagenase (metalloproteinase MMP-2) and elastase activities. The total phenolic and flavonoids contents were also determined. HPLC analysis revealed the presence of the flavonoids orientin and iso-orientin, which were quantified to be used as chemical markers. The results suggested that the extracts of C. pachystachya leaves present the potential to be used in dermocosmetic formulations to prevent the skin aging process, which attracts the attention of pharmaceutical companies and researchers interested in the development of novel ingredients likely to be used as active principles in antiaging products.

scholarly journals Manipulating Some Culturing Conditions Enhances Production of Solanine in Microshoots, Callus and Cell suspension Cultures of Solanum nigrum L.: A Wild Medicinal Plant

2020 ◽  
Vol 24 ◽  
pp. 51
Author(s):  
Rida A Shibli
Keyword(s):  
Light Intensity ◽  
Medicinal Plant ◽  
Cell Suspension ◽  
Growth Parameters ◽  
Cell Suspension Cultures ◽  
Solanum Nigrum ◽  
Callus Growth ◽  
Culture Techniques ◽  
Solanum Nigrum L

Solanum nigrum L. is a medicinal plant of solanaceae family with distinguished therapeutic properties. Traditionally, S. nigrum. had been used as an anti-tumorgenic, antioxidant, hepatoprotective, diuretic, and antipyretic agent. The most important alkaloid member in this plant is solanine. Therefore, this study was conducted to utilize tissue culture techniques for the enhancement of solanine production in the in vitro grown cultures of this promising neglected plant. For callus growth and development experimental part, the highest callus growth parameters (callus diameter (21.4 mm) and callus fresh weight (2202.4 mg) were obtained in callus grown on Murashige & Skoog MS media supplemented with 2,4-Dichlorophenoxyacetic acid (2.0 mg·L-1) plus 1.5 mg·L-1 Thidiazuron. Similar trend was also obtained in cell suspension culture experiment, as maximum growth was recorded at similar hormone combination. Moreover, High-performance liquid chromatography analysis revealed that, solanine was affected by growth regulator type and concentration. The highest solanine levels were obtained when the explants were treated with 6-benzylaminopurine at level of 2.0 mg·L-1, as solanine content reached up to (2.61, 1.53 mg.g-1) for callus and cell suspension, respectively, while, microshoot contained the highest solanine (4.52 mg.g-1 DW) at 6-benzylaminopurine level of 1.6 mg.L-1. Additionally, carbon source had positively affected solanine level, where 0.2 M sucrose resulted in production of the highest amounts (3.13, 2.03 and 1.20 mg.g-1 DW) of solanine in microshoots, callus and cell suspension, respectively. Also, exposing microshoots and callus to light intensity of (100 µmol.m -2 s-1) yielded the highest solanine content (4.03 and 1.26 mg.g-1 DW, respectively),while the lowest solanine levels (1.50 and 0.48 mg.g-1 DW) were observed in plant material exposed to the lowest light intensity treatment (25 µmol.m -2 s-1) .

scholarly journals Plantlets Regeneration from Crown Bud Slicing of Pineapple (Ananas comosus)

2018 ◽  
Vol 10 (3) ◽  
pp. 484-490 ◽  
Author(s):  
Zulkarnain Zulkarnain ◽  
Neliyati Neliyati ◽  
Eliyanti Eliyanti
Keyword(s):  
Tissue Culture ◽  
Adventitious Shoots ◽  
Culture Technique ◽  
Leaf Number ◽  
Ananas Comosus ◽  
Culture Techniques ◽  
Lateral Shoots ◽  
Four Levels ◽  
Tissue Culture Techniques

Pineapple propagation by lateral shoots, suckers or crowns is often confronted with limited number of regenerated seedlings and high diversity in flowering and fruit formation. In order to solve this problem, this study offer an alternative method by using tissue culture techniques. This study aimed to determine the effect of growth regulators on plantlet regeneration from bud slicing of pineapple cv. Tangkit. Four levels of 2.4-D (0.0, 0.001, 0.01 and 0.1 ppm) in combination with BA (0.0, 0.1, 1.0 and 10.0 ppm) were tested on solid MS medium. Cultures were incubated in total darkness for a week followed by transfer to 16-hour photoperiod. Results showed that explants treated with 2,4-D and/or BA succeeded in regenerating adventitious shoots. Average leaf number did not differ significantly among treatments (P = 0.60). Highest leaf number (2.99 ± 0.23) was obtained on medium with 0.01 ppm 2,4-D without BA, followed by 0.1 ppm 2,4-D without BA (2.85 ± 0.33). Meanwhile, roots were only formed on medium with 0.1 ppm 2.4-D without BA (4.2 ± 0.37 per shoot). Thus, complete plantlets were regenerated only on medium supplemented with 0.1 ppm 2,4-D without BA. The growth of plantlets was relatively uniform, and plantlet acclimatization succeeded 100% on Jiffy pots. The finding of optimum concentration of 2.4-D and BA in this study is important to develop standard protocol for in vitro propagation of pineapple cv. Tangkit. Thus, the benefit of producing seeds in large quantities and relatively uniform in growth is made possible through tissue culture technique.

scholarly journals Prunella vulgaris: A Comprehensive Review of Chemical Constituents, Pharmacological Effects and Clinical Applications

2019 ◽  
Vol 25 (3) ◽  
pp. 359-369 ◽  
Author(s):  
Su-Juan Wang ◽  
Xiao-He Wang ◽  
Yuan-Yuan Dai ◽  
Ming-Hua Ma ◽  
Khalid Rahman ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Current Knowledge ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Mechanisms Of Action ◽  
Clinical Applications ◽  
In Vivo Studies ◽  
Perennial Herb ◽  
Prunella Vulgaris

Prunella vulgaris (PV) is a perennial herb belonging to the Labiate family and is widely distributed in the northeastern Asian countries such as Korea, Japan, and China. It is reported to display diverse biological activities including anti-microbial, anti-cancer, and anti-inflammation as determined by in vitro or in vivo studies. So far, about 200 compounds have been isolated from PV plant and a majority of these have been characterized mainly as triterpenoids, sterols and flavonoids, followed by coumarins, phenylpropanoids, polysaccharides and volatile oils. This review summarizes and analyzes the current knowledge on the chemical constituents, pharmacological activities, mechanisms of action and clinical applications of the PV plant including its potential as a future medicinal plant. Although some of the chemical constituents of the PV plant and their mechanisms of action have been investigated, the biological activities of many of these remain unknown and further clinical trials are required to further enhance its reputation as a medicinal plant.

scholarly journals Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

2021 ◽  
Vol 21 (1) ◽  
pp. 54-57
Author(s):  
Dyah Nuning Erawati ◽  
Yusriatul Mawaddah ◽  
Siti Humaida ◽  
Irma Wardati
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Culture Techniques ◽  
Planting Material ◽  
Randomized Design ◽  
Wet Weight ◽  
The Mean ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

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