scholarly journals GC–MS analysis of ethanolic leaf extract of Capparis divaricata Lam.

2020 ◽  
pp. 34-35
Author(s):  
M. Hemalatha ◽  
Saivenkatesh Korlam ◽  
N. Yasodamma
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Medicinal Plants ◽  
Andhra Pradesh ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Ms Analysis ◽  
Pharmacological Studies ◽  
Based Medicine ◽  
Ethanolic Leaf Extract

Planet earth is rich in plant-based medicine. Unfortunately, most of the medicinal plants are not in lime light for their optimal utilisation by the mankind mostly, because of unknown medicinally important phytochemicals present in them. Capparis divaricata Lam. is one of the medicinally important plants widely distributed in Arthagiri Hills of Chittoor Dist., Andhra Pradesh. The plant is ethnobotanically used for different ailments especially cancer. In the present study, Gas Chromatography and Mass Spectrometry (GC-MS) analysis of leaf ethanolic extract of Capparis divaricate is reported. Octasilaxone, Hexadecamethyl; Acetoxymethyl – Trimethyl: Octamethyl were identified as the major compounds.  Further, these compounds may act as anti-cancerous agents by efficient pharmacological studies.

scholarly journals EVALUATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND CYTOTOXICITY OF ETHANOLIC LEAF EXTRACT OF ACACIA CAESIA (L.) WILLD. AGAINST HELA CELL LINE

2019 ◽  
Vol 12 (1) ◽  
pp. 520
Author(s):  
Sharmila S ◽  
Ramya E K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Wide Range ◽  
Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

scholarly journals EVALUATION OF GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND CYTOTOXICITY OF ETHANOLIC LEAF EXTRACT OF ACACIA CAESIA (L.) WILLD. AGAINST HELA CELL LINE

2019 ◽  
Vol 12 (1) ◽  
pp. 520
Author(s):  
Sharmila S ◽  
Ramya E K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Wide Range ◽  
Ethanolic Leaf Extract

Objective: The objective of this study is to analyze the bioactive compounds of the ethanolic leaf extract of Acacia caesia using gas chromatography-mass spectrometry (GC-MS) method and also screen the in vitro cytotoxic activity against HeLa-E 72 cancer cell line.Methods: The present research was carried out using GC-MS analysis, while mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Wiley library. Cytotoxicity was assessed with 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide assay, and cellular morphological alterations were studied using phase contrast inverted light microscope of 400×. The ethanol extract of A. caesia was screened for their cytotoxicity at different concentrations (12.5–200 μg/ml), to determine the mean percentage (%) cell viability.Results: The results of GC/MS analysis showed the presence of 41 major compounds. In terms of percentage amounts, 1,8-diphenyl-3,4,10,11- tetrahydro[1,4]dioxino[2,3-g:5,6-g’]diisoquinoline, 6-(chloromethyl)-4-(3,4-dimethoxy-2-(phenylmethoxy)-phenyl)-3-methyl-2-yridinecarboxylate, and 2’,4’,6’-Trinitro-5’-phenyl-1,1’:3’,1”-terphenyl were predominant in the extract and have the property of antioxidant, antidepressant potential, antibacterial activity, cytotoxic, diabetic, and induced brain activity. The results of cytotoxicity at highest concentration (200 μg/ml) of the cells became rounder, shrunken and showed signs of detachment from the surface of the wells denoting cell death.Conclusions: From this study, it is obvious that A. caesia leaf extracts contain various bioactive constituents with a wide range of medicinal properties which is used to treat multiple disorders and it also gives a detailed insight about the phytochemical profile which could be exploited for the development of plant-based drugs. Further, the ethanolic extract of A. caesia exhibits potent cytotoxic activity against HeLa-E 72 cell line.

scholarly journals IN VITRO ANTIBACTERIAL ACTIVITY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHANOLIC EXTRACT OF LEAVES OF ELETTARIA CARDAMOMUM L. MATON

2019 ◽  
Vol 12 (1) ◽  
pp. 73
Author(s):  
Prabu P ◽  
Edayadulla N ◽  
Anand S
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Elettaria Cardamomum ◽  
Ms Analysis ◽  
In Vitro Antibacterial Activity

Objective: The objective of the present study is to determine the possible phytoconstituents identified by gas chromatography and mass spectrometry (GC-MS) analysis of an ethanolic leaves extract of Elettaria cardamomum L. Maton.Methods: The extraction of E. cardamomum was done by cold solvent extraction system at room temperature. GC-MS analysis of lyophilized ethanolic leaves extract of plant samples was carried out by GC-MS-GC Clarus 500 Perkin Elmer. Results: In E. cardamomum, 21 phytochemicals were identified among which retinal, 9-cis-showed the highest area (44.86%) and benzeneethanamine, α-methyl- showed the lowest area (0.12%). The major compounds identified were retinal, 9-cis-, 1-heptatriacotanol, phytol, n-hexadecanoic acid, naphthalene,decahydro-1,1,4atrimethyl-6-methylene-5-(3-methyl-2-4-pentadienyl)-[4aS-(4aα,5α,8aα)]-, β-pinene, 2H-pytan-3-ol,6- ethenyltetrahydro-2,2,6-trimethyl-and cyclopropane, trimethanol, (2-methyl-1-propanylidene).Conclusion: GC-MS analysis revealed the presence of hydrocarbon alkane, ester, terpenes, phenolic compounds, steroids, and fatty acids in E. cardamomum. These active phytoconstituents contribute to the medicinal efficacy of the plant, and the plant can be used for the sourcing of these compounds.

scholarly journals Phytochemical screening and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Silybum marianum (L).

2019 ◽  
Vol 9 (1) ◽  
pp. 85-89 ◽  
Author(s):  
M Padma ◽  
S Ganesan ◽  
T Jayaseelan ◽  
S Azhagumadhavan ◽  
P Sasikala ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Silybum Marianum ◽  
Selective Sequential Extraction ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Objective: To investigate the phytochemicals and GC-MS analysis of ethanol extracts of Silybum marianum. Methods: The air-dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, ethanol to obtain an ethanolic extract. Then, each of the extracts was further subjected to gas chromatography-mass spectrometry. Results: Qualitative determination of the different biologically active compounds from crude extracts of Silybum marianum using gas chromatography-mass spectrometry revealed different types of high and low molecular weight chemical entities with varying amounts present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Conclusions: The study established the chemical composition and anticancer activity of the plant. Keywords: Silybum marianum, Phytochemicals screening, GC–MS analysis, Bioactive compounds.

scholarly journals GC-MS Analysis of the Ethanolic Extract of the whole Plant Drosera indica L.

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
K R Asha ◽  
S. Priyanga ◽  
S. Hemmalakshmi ◽  
K. Devaki
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Gas Chromatography ◽  
Mass Spectrum ◽  
Ethanolic Extract ◽  
Decanoic Acid ◽  
Ms Analysis ◽  
Whole Plant ◽  
Medicinal Properties ◽  
Benzene Dicarboxylic Acid

Aim: The present study is carried out to explore the phytoconstituents present in the ethanolic extract of the whole plant Drosera indica L. by GC-MS analysis. Methods: The ethanolic extract of the whole plant of Drosera indica L. is used for the GC-MS analysis. JEOL GCMATE II GC-MS (Agilent Technologies 6890 N Network GC system for gas chromatography) is used for the analysis. The compounds are identified by the gas chromatography coupled with the mass spectrometry. The molecular weight and structure of the compounds of test materials are ascertained by interpretation of the mass spectrum of GC-MS using the database of National Institute Standard and Technology (NIST). Results: GC-MS analysis of Drosera indica L. reveal the presence of the nine biological active compounds which include phytol, 4 methyl-4-nonadecene, 4 ’ ,5,7-Trihydroxyisoflavone,1,2-Benzene dicarboxylic acid, bis [2-methyl propyl] ester, Tetra decanoic acid, Eicosane, 2,6,10,14,18–pentamethyl, Tetracosane, Lochnerine and methyl–n-hexadecylketone. Conclusion: The results specify that the ethanolic extract of the whole plant, Drosera indica contains various bioactive compounds and therefore has various medicinal properties which can be used for the treatment of various diseases.

scholarly journals IN VITRO ANTIBACTERIAL ACTIVITY AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHANOLIC EXTRACT OF LEAVES OF ELETTARIA CARDAMOMUM L. MATON

2019 ◽  
Vol 12 (1) ◽  
pp. 73
Author(s):  
Prabu P ◽  
Edayadulla N ◽  
Anand S
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Elettaria Cardamomum ◽  
Ms Analysis ◽  
In Vitro Antibacterial Activity

Objective: The objective of the present study is to determine the possible phytoconstituents identified by gas chromatography and mass spectrometry (GC-MS) analysis of an ethanolic leaves extract of Elettaria cardamomum L. Maton.Methods: The extraction of E. cardamomum was done by cold solvent extraction system at room temperature. GC-MS analysis of lyophilized ethanolic leaves extract of plant samples was carried out by GC-MS-GC Clarus 500 Perkin Elmer. Results: In E. cardamomum, 21 phytochemicals were identified among which retinal, 9-cis-showed the highest area (44.86%) and benzeneethanamine, α-methyl- showed the lowest area (0.12%). The major compounds identified were retinal, 9-cis-, 1-heptatriacotanol, phytol, n-hexadecanoic acid, naphthalene,decahydro-1,1,4atrimethyl-6-methylene-5-(3-methyl-2-4-pentadienyl)-[4aS-(4aα,5α,8aα)]-, β-pinene, 2H-pytan-3-ol,6- ethenyltetrahydro-2,2,6-trimethyl-and cyclopropane, trimethanol, (2-methyl-1-propanylidene).Conclusion: GC-MS analysis revealed the presence of hydrocarbon alkane, ester, terpenes, phenolic compounds, steroids, and fatty acids in E. cardamomum. These active phytoconstituents contribute to the medicinal efficacy of the plant, and the plant can be used for the sourcing of these compounds.

scholarly journals PHYTOCHEMICAL STUDIES AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF SARCOSTEMMA BREVISTIGMA, WIGHT & ARN.

2017 ◽  
Vol 10 (3) ◽  
pp. 462 ◽  
Author(s):  
Dhivya S M ◽  
Kalaichelvi K
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Sarcostemma Brevistigma

ABSTRACTObjective: To explore the phytochemical constituents present in Sarcostemma brevistigma using gas chromatography-mass spectrometry (GC-MS).Methods: Preliminary phytochemical analysis of secondary metabolites was made by following standard procedures. GC-MS analysis of the plantextracts was performed by using GC-MS equipped with a DB-35MS capillary standard non-polar column and gas chromatograph interfaced to a MassSelective Detector (MS-DSQ-II) with Xcalibur software.Results: The qualitative phytochemical analysis of this species exhibited the presence of alkaloids, glycosides, flavonoids, phenols, steroids, aminoacids, tannins, terpenoids, quinones, and coumarin. The GC-MS analysis revealed the presence of 24 compounds in the ethanolic extract of aerial partof Sarcostemma brevistigma. The most prevailing compound was 14,17 bis(dimethylaminoamino)[3.3]paracyclophan-5,8-diacetonitrile (14.86%).Conclusion: The study concludes that the species Sarcostemma brevistigma is a potential source for bioactive compounds such as esters, alkanes,alcohols, alkenes, amide, amine, phenol, ketone, and so forth. This study justifies the traditional usage of this species.Keywords: Gas chromatography-mass spectrometry, Sarcostemma brevistigma, Phytochemical compounds, Medicinal plants.

scholarly journals Phytochemical Analysis of Caesalpinia pulcherrima (L.) Sw. Leaf Extract Using GC-MS Analysis

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Saravanan P.
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Folk Medicine ◽  
Phytochemical Analysis ◽  
Pharmacological Activities ◽  
Malarial Infection ◽  
Ms Analysis ◽  
Soxhlet Apparatus

Most of the worlds populations residing in developing countries depend on alternative medicine and use of plant ingredients. Caesalpinia pulcherrima (Fabaceae) is used in folk medicine for the treatment of various diseases including asthma, bronchitis, cholera, diarrhea, dysentery and malarial infection. The aim of the study to identified bioactive compounds presence in the chloroform leaf extract of Caesalpinia pulcherrima (L.) Sw. using Gas Chromatography and Mass Spectrometry (GC-MS) analysis. The leaf extraction was done by Soxhlet apparatus method. The GC-MS analysis has shown the presence of thirteen compounds in the chloroform leaf extract of Caesalpinia pulcherrima (L.) Sw. The identified bioactive compounds from this plant have been known to possess pharmacological activities.

scholarly journals GC-MS Analysis of Bioactive Compounds on Ethanolic Leaf Extract of Phyllodium pulchellum L. Desv.

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Velmurugan G. ◽  
Anand S. P.
Keyword(s):  
Gas Chromatography ◽  
Mass Spectrum ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Biological Activities ◽  
Ms Analysis ◽  
Phytochemical Compounds ◽  
Minor Compounds ◽  
Therapeutic Activities ◽  
Ethanolic Leaf Extract

The present investigation was carried out to characterize the bioactive compounds present in leaf extract of Phyllodium pulchellum using Gas Chromatography-Mass Spectrum (GC-MS). The results of the GC-MS analysis provide different peaks determining the presence of 10 phytochemical compounds with different therapeutic activities. The major phytocompounds 9-Octadecenamide, (Z) (18.89), 9-Cyclohexylnonadecane (15.93), 2,2,2-trifluoroethyl 2- methyltetrahydro-5-oxo-3-furancarboxylate (10.47) and minor compounds were also present. From the results, it could be concluded that P.pulchellum contains various bioactive compounds have various biological activities. Therefore, it is recommended as a plant of phytopharmaceutical importance.

scholarly journals The Analysis of Bayer Liquor by SPME-GC-MS of Derivatized Organic Poisons

Proceedings ◽  
2021 ◽  
Vol 57 (1) ◽  
pp. 101
Author(s):  
Virgil Badescu ◽  
Raluca Senin
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Bayer Liquor ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Silylating Agent

The aim of this article was the gas chromatography–mass spectrometry (GC-MS) analysis oforganic matter from a residual liquor sample (S.C. Alum S.A., Tulcea), extracted by the solid-phasemicroextraction method (SPMA) and derivatized with N-(tert-butyldimethylsilyl)-Nmethyltrifluoroacetamide(MTBSTFA) as the silylating agent. [...]

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