GC analysis of different parts of Tecoma stans (L.) Juss. ex Kunth for fatty acid composition

The production of neutral lipids and phospholipids by submerged cultures of the mushroom Tricholoma nudum, as well as the fatty acid composition of these two fractions, was studied as a function of time. The bulk of the neutral lipids was produced after 2 days when the organism appeared to be in a non-proliferative phase. The major fatty acids of the neutral lipids were palmitic, oleic, and linoleic acid (23–35% each); stearic acid was a minor component (8–13%); myristic, palmitoleic, and linolenic acid were present in small amounts (0.5–4.8%). The major fatty acid of the phospholipids was linoleic acid (55–70%); palmitic (15–19%), stearic (1.8–4.6%), and oleic (7–19%) acid were minor components; myristic, palmitoleic, and linolenic (0–2.3%) were present in small amounts. Linolenic acid was a major fatty acid (26–30%) only in the early stages of growth.A preliminary investigation was carried out with a 4-day-old culture to establish the identity of the various components of the neutral lipids and phospholipids. The neutral lipids were mainly triglycerides (92%). Small amounts of ergosterol esters (1%), free fatty acids (< 1%), ergosterol (1.7%), and unidentified non-saponifiable compounds were also present. The phospholipids contained phosphatidyl choline (59%) as the major component; phosphatidyl ethanolamine (26%), phosphatidyl serine and phosphatidic acid (7.8%), and an inositol containing phospholipid were minor components.Some of the techniques applied were specially developed for the present type of studies and are described in detail.

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INVESTIGATIONS INTO THE PRODUCTION OF LIPIDS BY SUBMERGED CULTURES OF THE MUSHROOM TRICHOLOMA NUDUM: I. FATTY ACID COMPOSITION OF NEUTRAL LIPIDS AND PHOSPHOLIPIDS AS A FUNCTION OF TIME

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y62-096 ◽

1962 ◽

Vol 40 (1) ◽

pp. 847-855 ◽

Cited By ~ 3

Author(s):

D. C. Leegwater ◽

C. G. Youngs ◽

J. F. T. Spencer ◽

B. M. Craig

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Linoleic Acid ◽

Linolenic Acid ◽

Acid Composition ◽

Neutral Lipids ◽

Minor Component ◽

Major Fatty Acid ◽

Submerged Cultures

The production of neutral lipids and phospholipids by submerged cultures of the mushroom Tricholoma nudum, as well as the fatty acid composition of these two fractions, was studied as a function of time. The bulk of the neutral lipids was produced after 2 days when the organism appeared to be in a non-proliferative phase. The major fatty acids of the neutral lipids were palmitic, oleic, and linoleic acid (23–35% each); stearic acid was a minor component (8–13%); myristic, palmitoleic, and linolenic acid were present in small amounts (0.5–4.8%). The major fatty acid of the phospholipids was linoleic acid (55–70%); palmitic (15–19%), stearic (1.8–4.6%), and oleic (7–19%) acid were minor components; myristic, palmitoleic, and linolenic (0–2.3%) were present in small amounts. Linolenic acid was a major fatty acid (26–30%) only in the early stages of growth.A preliminary investigation was carried out with a 4-day-old culture to establish the identity of the various components of the neutral lipids and phospholipids. The neutral lipids were mainly triglycerides (92%). Small amounts of ergosterol esters (1%), free fatty acids (< 1%), ergosterol (1.7%), and unidentified non-saponifiable compounds were also present. The phospholipids contained phosphatidyl choline (59%) as the major component; phosphatidyl ethanolamine (26%), phosphatidyl serine and phosphatidic acid (7.8%), and an inositol containing phospholipid were minor components.Some of the techniques applied were specially developed for the present type of studies and are described in detail.

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Effect of wilting and type of additive on the fatty acid composition of grass silage

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200007055 ◽

2002 ◽

Vol 2002 ◽

pp. 49-49

Author(s):

H.E. Warren ◽

J.K.S. Tweed ◽

S.J. Youell ◽

R. J. Dewhurst ◽

J.D. Wood ◽

...

Keyword(s):

Fatty Acids ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Polyunsaturated Fatty Acids ◽

Linolenic Acid ◽

Fatty Acid Profile ◽

Acid Composition ◽

Nutritional Value ◽

Red Clover ◽

Grass Silage

Forages, such as grass and red clover, are a rich source of n -3 polyunsaturated fatty acids, especially α-linolenic acid (C18:3n -3), and may be used as a method of improving the nutritional value of ruminant products. Silage is an important feed for cattle, therefore, a study was carried out to elucidate the effects of wilting and the use of additives on the fatty acid profile of the resultant silage.

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Composition of Stigmatic Exudate, Nectar and Pistil of Watermelon, Citrullus lanatus (Thunb.) Matsum. & Nakai, Before and After Pollination

Functional Plant Biology ◽

10.1071/pp9830257 ◽

1983 ◽

Vol 10 (3) ◽

pp. 257 ◽

Cited By ~ 3

Author(s):

JS Hawker ◽

M Sedgley ◽

BR Loveys

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Linolenic Acid ◽

Acid Composition ◽

Citrullus Lanatus ◽

Major Fatty Acid ◽

Quantitative Composition ◽

Before And After ◽

Stigmatic Exudate

The composition of stigmatic exudate, nectar, stigma tissue and style tissue was studied in watermelon flowers at 25/20°C (day/night) as the flowers aged after opening, and in unpollinated and pollinated flowers at 30/25°C. The stigmatic exudate contained fructose, glucose, sucrose, and polysaccharide which increased during ageing. Pollination also resulted in an increase in the secretion of these compounds by the stigma. Killed ethanol-extracted pollen was not as effective as live pollen, as less sugar and no polysaccharide was secreted. The fatty acid composition of stigma lipids did not change as a response to pollination. Linolenic acid was the major fatty acid in the lipids of stigmas, ovaries and pollen; high levels of lipids were present in pollen. The current results provide the quantitative composition of the stigmatic exudate which has been shown previously by microscopy to increase within minutes of pollination of watermelon flowers.

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FATTY ACID COMPOSITION OF THE PIG UTERUS, FETUS AND BELLY FAT

Canadian Journal of Animal Science ◽

10.4141/cjas79-026 ◽

1979 ◽

Vol 59 (1) ◽

pp. 211-213

Author(s):

D. W. FRIEND ◽

J. I. ELLIOT

Keyword(s):

Arachidonic Acid ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Soybean Oil ◽

Linolenic Acid ◽

Fatty Acid Profile ◽

Acid Composition ◽

Oil Content

The fatty acid composition of the uterus and fetuses from gilts killed 30 days after breeding at puberty was not affected by the soybean oil content (0–20%) of the diet given to the gilts before breeding. Differences in fatty acid profile were evident in belly fat samples taken from the same gilts. Although the belly fat contained linolenic acid, it did not appear to contain arachidonic acid as did the uterus and fetuses.

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