Effect of gamma rays and ethyl methane sulphonate (EMS) on yield and yield attributes of Cicer arietinum in M1 generation.

1970 ◽  
pp. 01
Author(s):  
L. Mullainathan ◽  
S. Umavathi
Keyword(s):  
Gamma Rays ◽  
Quantitative Traits ◽  
Mean Value ◽  
Ethyl Methane Sulphonate ◽  
Yield Attributes ◽  
Methane Sulphonate ◽  
Treated Plant ◽  
The Mean ◽  
Lethal Doses ◽  
Different Doses

A study was conducted to compare the effect of mutagens on yield and yield attributes of chickpea in M1 generation. In this regard, ‘CO-4’ variety of chickpea was subjected to different concentrations of gamma rays (20, 30, 40, 50 and 60kR) and EMS (10, 20, 30, 40 and 50mM) for inducing mutation. The effect of gamma rays and EMS with different doses/concentrations on yield and yield attributes were observed in M1 generation. From the result, it was observed that the mean value of all the quantitative traits of M1 generation showed a reduction upon enhancing the doses of mutagen. Mean performance in terms of these traits showed good qualities in comparison with the treated plant. The lethal doses were found in 40kR of gamma rays and 30mM of EMS and were carefully analyzed for further generations.

Quantitative variation induced by gamma rays, ethyl methane sulphonate, and hydrazine hydrate in mung bean (Phaseolus aureus Roxb.)

1984 ◽  
Vol 26 (4) ◽  
pp. 492-496 ◽  
Author(s):  
Irfan A. Khan
Keyword(s):  
Gamma Rays ◽  
Seed Weight ◽  
Genetic Advance ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Plant Yield ◽  
Mean Values ◽  
Methane Sulphonate ◽  
The Mean ◽  
100 Seed Weight

Induced variability was studied in the quantitative characters of mung bean (Phaseolus aureus Roxb.) after treatment with gamma rays, ethyl methane sulphonate (EMS), and hydrazine hydrate (HZ) in M1, M2, and M3 generations. The characters evaluated were seeds per pod, 100-seed weight, and total plant yield. The variability increased in almost all of the characters in the M1 generation. The mean number of seeds per pod and 100-seed weight decreased, whereas the plant yield did not show any particular trend. There was an increase in the mean values after gamma irradiation and EMS treatments in the M2 generation. However, HZ fails to show the same response. However, the mean values increased in the M3 generation. Estimates of heritability were higher for 100-seed weight, followed by plant yield, and then seeds per pod in the M3 generation. Coefficient of genotypic variation and genetic advance were high for the plant yield in M2 and M3 generations. In general, the genotypic coefficient of variation values, heritability, and genetic advance increased more in M3 as compared with M2, indicating that the significant gain could possibly be achieved through selection in M3 generation.Key words: quantitative, heritability, mutagenesis.

scholarly journals Morphology and Yield Parameters and Biochemical Analysis of Soybean (Glycine max (L.) Mrr.) Using Gamma Rays, EMS and DES Treatment

2015 ◽  
Vol 35 ◽  
pp. 50-58 ◽  
Author(s):  
P. Gopinath ◽  
P. Pavadai
Keyword(s):  
Glycine Max ◽  
Plant Height ◽  
Seed Yield ◽  
Gamma Rays ◽  
Fruit Size ◽  
Mean Value ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Yield Parameters ◽  
Methane Sulphonate

Soybean (Glycine max (L.) Merr.) var. Co-1 seeds were treated with physical mutagen namely such as gamma rays and chemical mutagen namely such as Ethyl Methane Sulphonate (EMS) and Diethyl Sulphate (DES). Various concentrations of gamma rays 10, 20, 30, 40, 50 and 60 KR, EMS (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %) and DES (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %). The morphological parameters such as days to first flower, plant height, number of cluster per plant, number of pod per plan, seed yield per plant, number of branches per plant, number of leaves per plant, protein content and oil content increasing with increasing level of some doses gamma rays, Ethyl methane sulphonate and Diethyl sulphate treatment in M2 and M3 generation were carried out. A number of mutants were recorded in M2 and M3 generations for one or more traits viz., plant height, maturity, branching, pigmentation, fruit size and yield were isolated in M2 and M3 generation. Mean for various characters increased at mutagenic treatment than control. The yield parameters like plant height, number of cluster per plant, number of seeds per plant and seed yield per plant were recorded the moderated and high mean value in the 50 KR of gamma rays 0.5% of EMS and 0.4% of DES treated population with compared to control plants.

scholarly journals Induced mutagenesis in Chickpea (Cicer arietinum (L.) with special reference to the frequency and spectrum of chlorophyll mutations

2016 ◽  
Vol 1 (1) ◽  
pp. 49 ◽  
Author(s):  
S. Umavathi ◽  
L. Mullainathan
Keyword(s):  
Cicer Arietinum ◽  
Gamma Rays ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Cicer Arietinum L ◽  
Chlorophyll Mutations ◽  
Methane Sulphonate ◽  
Chlorophyll Mutants ◽  
Induced Mutagenesis ◽  
Different Doses

A relative study of frequency and spectrum of chlorophyll mutations induced by mutagens in M2 generation was made with chickpea (Cicer arietinum (L). Variety ‘CO-4’. The treatments include different doses/concentrations of Gamma rays (20, 30, 40, 50 and 60kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM). From the study, the overall frequencies and spectrum of five types of induced chlorophyll mutants Viridis (0.55), Xantha (0.46), Chlorina (0.45), Albina (0.43) and Tigrina (0.35) were observed. The frequency of chlorophyll mutation was increased with increasing concentrations up to a level, beyond it declined in both the mutagens. And the chlorophyll frequency was found in the order of viridis > xantha >chlorina> Albina >tigrina. The chemical mutagen, EMS was found to be more effective in inducing chlorophyll mutations than gamma rays in Chick pea.

scholarly journals Gamma rays, EMS and DES induced changes on cytology of bhendi [Abelmoschus esculentus (L.) Moench]

2017 ◽  
Vol 1 ◽  
pp. 19
Author(s):  
R. Elangovan and P. Pavadai
Keyword(s):  
Gamma Rays ◽  
Mutation Breeding ◽  
Abelmoschus Esculentus ◽  
Ethyl Methane Sulphonate ◽  
Chemical Mutagens ◽  
Methane Sulphonate ◽  
Successful Approach ◽  
Induced Changes ◽  
Physical And Chemical ◽  
Different Doses

Mutation breeding in crop plants such as bhendi (Abelmoschus esculentus (L). Moench) is a successful approach in change of product having narrow genetic base. In the present study to the determine the effect of physical mutagen such as gamma rays and chemical mutagens such as Ethyl methane sulphonate (EMS) and Diethyl sulphate (DES) were used. The seeds were treating with different doses/concentration of Gamma irradiation (10KR, 20KR, 30KR, 40KR, 50KR and 60KR), EMS and DES (0.1, 0.2, 0.3, 0.4, 0.5, and 0.6%) for six hours were applied to 200 seed sample of each concentration and one respective control on bhendi. plants of four generations viz., M1, M2, M3 and M4. The cytological analysis, for example, chromosomal number was watched and recorded for images. The maximum changes of chromosome were observed in 50 KR of gamma rays and 0.4 % of EMS treatments than the other physical and chemical mutagens. The length and shape of chromosome for varied in treated plants than the untreated plants.

scholarly journals Studies on the effects of mutagens on cytotoxicity behaviour in Pigeon pea (Cajanus cajan (L.) Millsp) Var.CO-7.

2016 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
M. Ariraman ◽  
T. Bharathi ◽  
D. Dhanavel
Keyword(s):  
Gamma Rays ◽  
Cajanus Cajan ◽  
Pigeon Pea ◽  
Ethyl Methane Sulphonate ◽  
Relative Deviation ◽  
Methane Sulphonate ◽  
Deviation Rate ◽  
Different Types ◽  
Rate Frequency ◽  
Different Doses

The cytological studies provide more information regarding the response of a genotype to the particular mutagen and also provide chances to select desirable characters. The seeds of pigeon pea were subjected to different doses and concentrations of gamma rays and ethyl methane sulphonate (EMS). The effects of different mutagenic treatments on mitosis chromosomal behavior have been studied in both the mutagens. Different types of mitotic aberrations like stickiness, precocious movement, bridge, clumping of chromosome and laggards, etc., were observed in all the treatments. However, the gamma rays treatments proved to be more effective in inducing mitotic aberrations as compared to EMS. The frequency of laggard was high when compared to other mitotic aberrations. The reduction in mitotic index and relative deviation rate frequency were observed with increase in doses and conc. of both the mutagenic treatments and gamma rays were found to be more effective than EMS treatments.

scholarly journals EFFECT OF MUTAGENS ON QUANTITATIVE CHARACTERS IN M3 GENERATION OF PEARL MILLET (PENNISETUM TYPHOIDES (BURN.F) STAPF. AND C.E. HUBB.)

1970 ◽  
pp. 01-05
Author(s):  
K. Ambli, L. Mullainathan
Keyword(s):  
Pearl Millet ◽  
Gamma Rays ◽  
Quantitative Traits ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Quantitative Characters ◽  
Methane Sulphonate ◽  
Weight Yield ◽  
Number Of Leaves ◽  
Higher Doses

A study was conducted to estimate the effects of gamma rays and ethyl methane sulphonate (EMS) on mutagenesis of Pearl millet. Gamma rays (10, 20 and 30kR) and EMS (20, 30 and 40mM) treatments were done to find out different quantitative traits viz., Days to first bloom, Plant height, Number of leaves, Number of nodes, Length of earhead, Breadth of earhead, 1000 grains weight, Yield per plant, Chlorophyll and carbohydrate content. These parameters were better in 20kR of gamma rays and 30mM of EMS when compared with the control and other doses/concentrations and higher doses decreased the parameters.

scholarly journals Induced Genetic Variability for Quantitative Traits in Pigeonpea

2020 ◽  
Author(s):  
S. M. Sangle
Keyword(s):  
Genetic Variability ◽  
Gamma Rays ◽  
Quantitative Traits ◽  
Control Plant ◽  
Ethyl Methane Sulphonate ◽  
Plant Populations ◽  
Ethyl Methane ◽  
Mean Values ◽  
Quantitative Characters ◽  
Methane Sulphonate

Seeds of two pigeonpea varieties namely BDN 708 and BSMR 853 were treated with Gamma rays, ethyl methane sulphonate (EMS) and Sodium azide (SA). The treated as well as control plant populations were screened to study the induced variability for quantitative characters. Positive shift in mean values for number of primary branches per plant was observed except 0.10% EMS in variety BSMR 853 and BDN 708 in M2 generation. The effect of all the mutagenic treatments on pods per plant revealed statistically significant negative as well as positive shifts in mean values in BSMR 853 in M2 and M3 generations.

scholarly journals Screening for pod shattering in mutant population of mungbean (Vigna radiata (L.) Wilczek)

2017 ◽  
Vol 9 (3) ◽  
pp. 1787-1791 ◽  
Author(s):  
N. Vairam ◽  
S. Anandhi Lavanya ◽  
C. Vanniarajan
Keyword(s):  
Gamma Rays ◽  
Vigna Radiata ◽  
Visual Observation ◽  
Induced Mutations ◽  
Ethyl Methane Sulphonate ◽  
Pod Shattering ◽  
Indian Agriculture ◽  
Methane Sulphonate ◽  
Important Objective ◽  
Pod Dehiscence

Mungbean, (Vigna radiata (L.) Wilczek) occupies a unique position in Indian agriculture and has been grown under various agro-ecological conditions. It is cultivated in 1.61mha with production of 3.38MT and productivi-ty of 474kg/ha in India. Mungbean pods are thin and brittle when dry, so shattering is a major problem. The loss of seeds by pod dehiscence is one of the major reasons for low yield in mungbean; thus, reducing the frequency of pod dehiscence is an important objective in mungbean breeding. Induced mutations, have offered a single and short alternative to conventional breeding including isolation, screening, selection and testing generation after generation. In this study, variability was induced by gamma rays and Ethyl methane sulphonate (EMS) in two greengram geno-types viz., CO (Gg) 7 and NM 65. Screening for pod shattering was carried out in M2 and M3 populations of green-gram. The scoring for shattering was recorded at physiological maturity of the pod. The shattering percentage ranged from 14.56 (400 Gy) to 93.45 per cent (20 mM). A total of 100 shattering tolerant mutants were selected from field based on visual observation. These mutants were again scored under laboratory condition as per IITA method. A total of 12 mutants of CO (Gg) 7 and 10 mutants of NM 65 which were tolerant to pod shattering were identified in M2 generation and forwarded to M3 generation. These mutants were scored for pod shattering under laboratory con-dition and nine mutants viz., M26, M44, M46, M58, M70, M71, M84, M92 and M98 were found to be tolerant in M3 generation. This study on identification and screening of the mutants tolerant to pod shattering with high yielding potential will help to increase the production of the pods to a greater extent.

scholarly journals Induced Mutagenesis in Cicer arietinum

2014 ◽  
Vol 12 ◽  
pp. 1-4 ◽  
Author(s):  
L. Mullainathan ◽  
S. Umavathi
Keyword(s):  
Cicer Arietinum ◽  
Gamma Rays ◽  
Crop Improvement ◽  
Ethyl Methane Sulphonate ◽  
Chick Pea ◽  
Biological Damage ◽  
Methane Sulphonate ◽  
Dose Concentration ◽  
Induced Mutagenesis ◽  
Effectiveness And Efficiency

The traditional varieties of chick pea have low potentiality and restricted variability with respect to economic characters. Broadening the genetic base for crop improvement can be quickly achieved through induced mutagenesis. The present study was undertaken in order to comparing the effectiveness and efficiency of mutagens on Cicer arietinum. In this regard, Co – 4 variety of chick pea was subjected to different dose/concentration of Gamma rays (20, 30, 40, 50 and 60 kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM) for inducing mutation. Mutagenic effectiveness and efficiency was calculated based on biological damage in M1 and chlorophyll mutations in M2. The results indicated that, mutagenic effectiveness increased with the increase in dose/concentration of mutagen. Intermediate treatments in general were found more efficient in causing less biological damage and inducing maximum amount of mutations. It shows that the chemical mutagens are more effective and efficient than physical mutagen for inducing mutation in Chick pea

Sign in / Sign up

Export Citation Format

Share Document