ANTIFUNGAL EFFICACY OF SOME INDIGENOUS PLANTS EXTRACT AGAINST RHIZOPUS STOLONIFER, THE CAUSAL AGENT OF WET ROT DISEASE OF POTATO (SOLANUM TUBEROSUM)

1970 ◽  
pp. 01
Author(s):  
Tijjani Ahmadu ◽  
Abdulaziz Bashir Kutawa ◽  
Jafar Sani Adam ◽  
Muhammad Abdulhadi ◽  
Mohammed Iliya
Keyword(s):  
Solanum Tuberosum ◽  
West African ◽  
Control Treatment ◽  
Syzygium Aromaticum ◽  
Rhizopus Stolonifer ◽  
Indigenous Plants ◽  
Piper Guineense ◽  
Mycelia Growth ◽  
Rot Disease ◽  
Plants Extract

Potato, (Solanum tuberosum (L.)) is a valuable stable food grown for its edible starchy tuber. This work was aimed to test the efficacy of indigenous plants extract for controlling wet rot disease of potato caused by Rhizopus stolonifer. The fungi was isolated from decaying bread and grown on potato dextrose agar (PDA) plates. Ripe Ginger (Zingiber officinale) (rhizome), West African pepper (Piper guineense) flower, Clove (Syzygium aromaticum) seed were collected from Muda Lawal Market, Bauchi, Nigeria. The dried seed were grinded using blender to get ginger, clove and West African pepper powder. Radial mycelia growth of Rhizopus stolonifer varied significantly (p≤0.01) with the application of different plant extracts. Mycelia growth of Rhizopus stolonifer was found to be low in potatoes treated with West African pepper (0.26 cm) than those treated with ginger (0.32 cm) and clove (0.33 cm). However, as observed from the result, ginger and clove did not differ statistically in their effect on Rhizopus mycelia growth (in vitro) but showed a better effect than the control treatment (0.40 cm). Inhibition of the mycelia growth by the West African pepper seed extract is an indication that it is fungicidal.

Two new defensive constituents from potato tubers (Solanum tuberosum)

2017 ◽  
Vol 72 (6) ◽  
pp. 393-396
Author(s):  
Liangyan Liu ◽  
Jun Han ◽  
Yong Shen
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Quantum Chemical ◽  
Spectroscopic Analysis ◽  
2D Nmr ◽  
Moderate Activity ◽  
Potato Tubers ◽  
Mycelia Growth ◽  
Late Blight Disease ◽  
Blight Disease

AbstractTwo new defensive constituents, solatuberenol A (1) and 3-O-β-d-glucopyranosyl stigmasta-5(6),24(28)-diene (2), were isolated from the potato tubers (Solanum tuberosum) infected with late blight disease. Their structures were identified by extensive spectroscopic analysis, including HRMS, IR, UV, 1D/2D NMR, ECD and quantum chemical calculations. Compounds 1 and 2 showed moderate activity against Phytophthora infestans with mycelia-growth inhibition of 30.1% and 52.4%, respectively, at the concentration of 500 ppm.

scholarly journals Teknik Inokulasi Buatan Clavibacter michiganensis subsp. sepedonicus, Penyebab Penyakit Busuk Cincin Bakteri, pada Tanaman Kentang (Solanum tuberosum L.)

Agrikultura ◽  
2016 ◽  
Vol 27 (2) ◽  
Author(s):  
Luciana Djaya ◽  
Ineu Sulastrini ◽  
Iin Rusita
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Block Design ◽  
Clavibacter Michiganensis ◽  
Bacterial Ring Rot ◽  
Seed Tubers ◽  
Clavibacter Michiganensis Subsp ◽  
Ring Rot ◽  
Inoculation Techniques ◽  
Rot Disease

ABSTRACT Inoculation Techniques of Clavibacter michiganensis subsp. sepedonicus, the Cause of Bacterial Ring Rot Disease, on Potato (Solanum tuberosum L.). Clavibacter michiganensis subsp. sepedonicus, the cause of bacterial ring rot disease on potatoes, has been detected in potato fields in Pangalengan. To anticipate the spread of the pathogen, researches on the desease epidemiology are urgent to be carried out. Artificial inoculation techniques will be useful in the epidemiological studies. The objective of this reasearch was to evaluate some inoculation techniques, which are simple, cheap and fast in causing disease symptoms. The experiment was carried out at the laboratory and glasshouse of Balai Penelitian Tanaman Sayuran (Balitsa), Lembang. The experiment was arranged in the randomized block design with five treatments of inoculation technique and five replications. The treatments were (a) soaking wounded seed tubers in pathogen suspension, (b) soaking not wounded seed tubers in pathogen suspension, (c) pathogen suspension was injected into leaf axil, (d) pathogen suspension was injected into seed tubers, and (e) pathogen suspension was poured into the planting holes. The results showed that stabbing and soaking tubers in pathogen suspension caused the shortest incubation period (17 days after inoculation) and the highest disease incidence (60%). Keywords : Clavibacter michiganensis subsp. sepedonicus, Bacterial ring rot, Potatoes, Inoculation techniquesABSTRAKClavibacter michiganensis subsp. sepedonicus (Cms), penyebab penyakit busuk cincin bakteri pada tanaman kentang, telah terdeteksi keberadaannya pada pertanaman kentang di Pangalengan. Dalam upaya mencegah penyebaran penyakit busuk cincin bakteri di Indonesia, perlu adanya studi epidemiologi patogen tersebut. Pada penelitian epidemiologi akan diperlukan cara menginokulasi tanaman secara buatan. Tujuan dari penelitian ini adalah untuk mendapatkan teknik inokulasi buatan yang dapat menyebabkan periode inkubasi yang lebih singkat dan persentase kejadian penyakit busuk cincin bakteri paling tinggi pada tanaman kentang. Percobaan dilaksanakan di rumah kaca dan laboratorium penyakit Balai Penelitian Tanaman Sayuran (Balitsa), Lembang. Percobaan dirancang secara rancangan acak kelompok dengan lima perlakuan teknik inokulasi Cms dan lima ulangan, yaitu inokulasi dengan (a) merendam ubi benih yang telah dilukai dalam suspensi bakteri, (b) merendam ubi benih tanpa dilukai dalam suspensi bakteri, (c) suspensi bakteri ditusukkan pada ketiak daun tanaman kentang, (d) suspensi bakteri ditusukkan pada ubi benih, dan (e) suspensi bakteri disiramkan pada lubang tanam. Masing-masing ulangan terdiri dari lima tanaman. Hasil penelitian menunjukkan bahwa dari lima teknik inokulasi buatan yang dilakukan, inokulasi dengan melukai ubi benih dan merendamnya dalam suspensi patogen menghasilkanJurnal Agrikultura 2016, 27 (2): 66-71ISSN 0853-2885Teknik Inokulasi Buatan Clavibacter….67periode inkubasi tersingkat, yaitu 17 hari setelah inokulasi, dan persentase kejadian penyakit tertinggi yaitu sebesar 60%.Kata Kunci : Clavibacter michiganensis subsp. sepedonicus, Busuk cincin bakteri, Kentang, Inokulasi buatan

scholarly journals Antagonistic effectiveness of some bacteria against Fusarium graminearum causing crown rot disease on wheat (Triticum aestivum)

2021 ◽  
Vol 13 (1) ◽  
pp. 69-80
Author(s):  
Majida Hadi Mahdi Alsaady ◽  
Hussein Ali Salim ◽  
Rakib A. Al-ani ◽  
Hadi M. Aboud ◽  
Jamal Talib M Al Roubaie
Keyword(s):  
Fusarium Graminearum ◽  
Culture Method ◽  
Dry Weight ◽  
Antagonistic Effect ◽  
Crown Rot ◽  
Control Treatment ◽  
Enzyme Analysis ◽  
Dual Culture ◽  
Rot Disease

In this study, the antagonistic effect of five bacteria genera namely Bacillus, Pseudomonas, Azotobacter, Azospirillum, and Streptomyces isolated from rhizosphere of wheat were evaluated against Fusarium graminearum as potential biocontrol agents in vitro. F. graminearum was molecularly diagnosed using the Polymerase chain reaction (PCR) technique. Each bacteria were tested for the production of catalase enzyme, oxidase enzyme, analysis of starch, analyze of gelatin, and the motility, where Azotobacter, Azospirillum, and Bacillus subtilis were positive for all tested. Fungal inhibition tests were performed by using the dual culture method and agar well diffusion technique. Among them, Streptomyces and Azospirillum exhibited potent inhibition to the growth of F. graminearum (72.14% and 66.42%) respectively, followed by B.pumillus, P.fluorescens, B. subtilis and Azotobacter ( 58.28%, 43.23%, 39.71% and 35.71%) respectively as compared with the control treatment (0.0%).The dry weight of the fungus biomass was decreased with bacteria P. fluorescens, Streptomyces sp, Azotobacter sp, Azospirillum sp, B. subtilis, and B. pumillus which reached (0.114, 0.103, 0.147, 0.101, 0.143, and 0.107 g) respectively compared to the control treatment that was 0. 665 g.

scholarly journals Assessment of Sarocladium oryzae Growth Inhibition, the Causal Agent of Rice Sheath Rot Disease, Using Methanol Extract of Binahong Leaves

2019 ◽  
Vol 2 (1) ◽  
pp. 15
Author(s):  
Endah Yulia ◽  
Silviya Rahma Dhiya Syafira ◽  
Fitri Widiantini ◽  
Wawan Kurniawan
Keyword(s):  
Growth Inhibition ◽  
Leaf Extract ◽  
Plant Protection ◽  
Colony Growth ◽  
Leaf Sheath ◽  
Control Treatment ◽  
Botanical Pesticide ◽  
Plant Pest ◽  
Rot Disease ◽  
Sheath Rot

Sheath rot disease caused by Sarocladium oryzae is an important disease on rice. The pathogen that infects uppermost leaf sheath enclosing to the young panicles can cause yield losses up to 85%. Though the disease is commonly controlled using synthetic fungicides, the use of natural products that safer to the environment have been enormously explored. The research aimed to test growth inhibition of S. oryzae colony using methanol leaf extract of binahong (Anredera cordifolia). The research was carried out from June until December 2017 at the Laboratory of Biotechnology Plant Protection, Department of Plant Pest and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The method used was Randomized Complete Design consisted of six treatments and four replications. The treatments were five concentrations of binahong leaf extract of 0.125%, 0.25%, 0.5%, 1%, 2% and one control treatment. The result showed that binahong leaf extract did not inhibit the growth of S. oryzae. The highest inhibition percentage of 4.55% at 0.25% concentration was considered to be a low inhibition category. Although the colony growth of S. oryzae was not significantly inhibited, the extract caused hyphal abnormalities. This actually shows the antifungal potency of the binahong leaf extract that needed further examination with more adequate methods.Keywords : Anredera cordifolia, botanical pesticide, rice sheath rot

scholarly journals Iturin A Induces Resistance and Improves the Quality and Safety of Harvested Cherry Tomato

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6905
Author(s):  
Mengxi Jiang ◽  
Xinyi Pang ◽  
Huawei Liu ◽  
Fuxing Lin ◽  
Fengxia Lu ◽  
...  
Keyword(s):  
Phenylalanine Ammonia Lyase ◽  
Tomato Fruit ◽  
Soft Rot ◽  
Cherry Tomato ◽  
Rhizopus Stolonifer ◽  
Storage Quality ◽  
Iturin A ◽  
Rot Disease ◽  
Important Disease

The soft rot disease caused by Rhizopus stolonifer is an important disease in cherry tomato fruit. In this study, the effect of iturin A on soft rot of cherry tomato and its influence on the storage quality of cherry tomato fruit were investigated. The results showed that 512 μg/mL of iturin A could effectively inhibit the incidence of soft rot of cherry tomato fruit. It was found that iturin A could induce the activity of resistance-related enzymes including phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD), glucanase (GLU), and chitinase (CHI), and active oxygen-related enzymes including ascorbate peroxidases (APX), superoxide dismutases (SOD), catalases (CAT), and glutathione reductase (GR) of cherry tomato fruit. In addition, iturin A treatment could slow down the weight loss of cherry tomato and soften the fruit. These results indicated that iturin A could retard the decay and improve the quality of cherry tomato fruit by both the inhibition growth of R. stolonifera and the inducing the resistance.

scholarly journals Proximate and Elemental Analysis of Local Spices Used in Nigeria

2019 ◽  
pp. 1-9
Author(s):  
Lydia M. Okorafor ◽  
Ishaq S. Eneji ◽  
Rufus Sha’Ato
Keyword(s):  
Mineral Elements ◽  
Syzygium Aromaticum ◽  
Crude Fibre ◽  
Piper Guineense ◽  
Edxrf Analysis ◽  
Significant Difference ◽  
Mineral Components ◽  
The Mean ◽  
Monodora Myristica ◽  
Xylopia Aethiopica

Comparative study of the proximate and mineral components of five local spices (Denniettia tripetala, Monodora myristica, Piper guineense, Syzygium aromaticum, Xylopia aethiopica and) were investigated.  The proximate composition revealed that the spices had considerable carbohydrate ranging from 26.2 –  53.4%, crude fibre 13.6 – 23.7%, crude protein 5.67 – 10.9%, but low ash 2.41 – 5.56%, moisture 8.61 – 17.7%  and fat 2.34 – 24.3%; except D.tripetala  and M. myristica with high moisture content of 17.7%  and crude fat content of   24.3%, respectively.  The EDXRF analysis shows the presence of mineral elements in the order of K ˃ Ca ˃ Fe ˃ Zn ˃ Sr > Se ˃ Mo ˃ Cu ˃ Mn ˃ Br > Rb. Statistical analysis indicates that there is no significant difference (α = 0.01, 0.05) in the mean contribution of the various sample. However, there is a relationship between the constituents of P.guineense and S. aromaticum, X. aethopica, D. tripetala and the elements (Mn, Br) and (Se, Zn). Generally, the findings indicate that the five spices are good sources of nutrients and mineral elements which could be exploited as great potentials for drugs and/or nutritional supplements.

scholarly journals Ethnopharmacological Survey and Physiological Evaluation of Nutritional and Phytochemical Contents of Indigenous Plants Used for Treatment of Toothache and Mouth Odour in Ijebu Ode Local Government Area, Ogun State, Nigeria

2020 ◽  
pp. 3559-3576
Author(s):  
OJEWUMI A. W. ◽  
OYEBANJI E.O.
Keyword(s):  
Local Government ◽  
Sampling Technique ◽  
Local Government Area ◽  
Crude Fibre ◽  
Indigenous Plants ◽  
Piper Guineense ◽  
Nutrient Contents ◽  
Ogun State ◽  
Analysis System ◽  
Level Of Significance

The use of herbs for improvement of oral hygiene is increasing in many communities in Nigeria despite the usage of other dental care products. On the basis of this the present study was conducted to assess indigenous plants used to manage dental condition and mouth odour and to evaluate nutritional, phytochemicals and antinutrient content of the plants. A survey was conducted to document plants used for treatment of toothache and mouth odour in Ijebu-Ode Local Government Area. Using random sampling technique, a total of one hundred structured questionnaire was administered to Traditional Health Practitioners in three major markets of the study area. Nutritional, phytochemical and anti-nutrient contents of most used plants were evaluated. Data were analyzed using Statistical Analysis System. Means were separated using Duncan’s Multiple Range Test at 5% level of significance (p < 0.05). A total of twenty-three (23) plants belonging to sixteen families were enumerated out of which Capsicum frutescens, Piper guineense fruits and Zanthoxylum zanthoxloides roots were the most exploited for management of the conditions. Crude fibre (8.86 %), fat (1.12%) and ash contents (4.73%) were significantly (p < 0.05) higher in Z. zanthoxloides roots while moisture (25.75 %) was significantly higher (p <0.05) in C. frutescens fruits. Calcium (192.10 mg/100g), phosphorus (108.50 mg/100g), sodium (51.33 mg/100g), iron (4.85 mg/100g), Zinc (3.94 mg/100g), manganese (1.15 mg/100g) and copper (2.12 mg/100g) were significantly (p < 0.05) higher in Z. zanthoxloides roots compared C. frutescens and P. guineense fruits. Vitamin A (600.00 µ/100g), vitamin B (0.07.00 mg/100g), vitamin C (94.54 mg/100g) and vitamin E (720.00) were significantly (p < 0.05) higher in C. frutescens than Z. zanthoxloides and P. guineense. Results also showed that tannin (6.40 %), oxalate (30.00%), phytate (0.40%) and trypsin inhibitor (20.00 %) were significantly higher in frutescens than Z. zanthoxloides roots. Similar significant (p<0.05) increase were observed in the quantity of flavonoid (3.25%), saponnis (1.30%), phenol (0.60%) and anthocyaninns (0.23%) in Z. zanthoxloides compared with C. frutescens and P. guineense.

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