Macroscopic in vitro analysis of titanium and fiberglass posts cemented in human premolars subjected to high temperatures for forensic purposes

SUMMARYObjective: To describe the macroscopicchanges of titanium and fiberglass postscemented in human premolars subjectedto high temperatures for forensic purposes.Materials and methods: An in Vitro experimentalstudy was conducted to observethe macroscopic physical changes of dentaltissues (enamel, dentine and cement), ofmaterials of conventional endodontic use(gutta-percha Wave One® Maillefer Dentsply®, endodontic cement with epoxic resinTop Seal® Maillefer Dentsply®), postscement Relyx TM ARC 3M ESPE®) andof the titanium posts (Tenax® EndodonticPost System Coltene®) and fiberglassposts (Tenax® Fiber Trans Coltene®) in124 human teeth, exposed to five ranks oftemperature 200ºC, 400ºC, 600ºC, 800ºC,1000ºC (three teeth at each temperature).Results: The studied tissues and dentalmaterials used in this study, offer greatresistance to high temperatures, exhibitingconsiderably variation of their macrostructure,in a way that the physical changes(dimensional stability, fissures, cracks,fractures, texture, color, carbonization andincineration) can serve to identify them andto associate such changes to each rank ofspecific temperature.Conclusion: The titanium and fiberglassposts cemented in human premolars offergreat resistance to the action of high tem-peratures. In the same way, they presentspecific changes associated to the dentaltissues that can contribute with the processof identification and medico-legal necropsyof a corpse or burned, incinerated or carbonizedhuman rests.Key words: Forensic dentistry, humanidentification, dental tissues, dental materials,endodontics, high temperatures.

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Stereomicroscopic analysis of dental materials employed in endodontic exposed to high temperatures

Revista Estomatología ◽

10.25100/re.v19i2.5728 ◽

2017 ◽

Vol 19 (2) ◽

Author(s):

Johanna Aramburo ◽

Angela Zapata ◽

Sugey Zúñiga ◽

Freddy Moreno

Keyword(s):

High Temperatures ◽

Dental Materials ◽

In Vitro Study ◽

Human Teeth ◽

Great Resistance ◽

Dental Tissues ◽

Specific Rank ◽

Hard Dental Tissues ◽

Physical Changes

Objective: To describe the physical estereomicroscopical changes that occur in dental tissues and materials employed in a conventional endodontic treatment after application of high temperatures. Materials and methods: An in Vitro study was carried out to observe microscopic, structural and physical changes on hard dental tissues (enamel, dentine and cement) and on endodontic materials (gutta-percha Maillefer Dentsply®, endodontical cement based on oxide of zinc-eugenol Eufar®, cement based on epoxic resin Top Seal® Dentsply®, glass ionomer Fuji I® GC America®, silver amalgam GS80® SDI® and composite Point 4® Kerr®) in 124 human teeth, submitted to five temperature ranks (200ºC, 400ºC, 600ºC, 800ºC, 1000ºC). Results: The studied tissues and dental materials presented great resistance to the high temperatures without varying considerably their micro structure, such as that the physical changes (dimensional stability, cracks, pits, fractures, texture, color, carbonization and incineration) can be observed through estereomicrophotographies and can be associated to each specific rank of temperature. Conclusions: Some macrostructural changes of dental tissues and the dental materials occur of specific form in each rank of temperature, and for this reason they can be employed in the comparison before and postmortem during the process of identification of burned or charred corpse and human remains. Key words: Forensic dentistry, human identification, stereomicroscopy, dental materials, high temperatures.

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In vitro analysis of thermocompaction time and gutta-percha type on quality of main canal and lateral canals filling

Brazilian Oral Research ◽

10.1590/s1806-83242010000300006 ◽

2010 ◽

Vol 24 (3) ◽

pp. 290-295 ◽

Cited By ~ 10

Author(s):

André Luiz da Costa Michelotto ◽

Cacio Moura-Netto ◽

Ângela Toshie Araki ◽

Eduardo Akisue ◽

Abílio Albuquerque Maranhão Moura ◽

...

Keyword(s):

Gutta Percha ◽

Main Canal ◽

Vitro Analysis ◽

In Vitro Analysis

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The Efficacy of Reciproc Blue® For Canal Cleanliness Following the Retreatment of Maxillary First Molar: In Vitro Analysis

Malaysian Journal of Science Health & Technology ◽

10.33102/mjosht.v7i1.142 ◽

2021 ◽

Vol 7 (1) ◽

pp. 22-27

Author(s):

Mohd Nazrin Isa ◽

Aws Hashim Ali Al-Kadhim ◽

Azlan Jaafar

Keyword(s):

Filling Material ◽

Volume Percentage ◽

Gutta Percha ◽

Maxillary Molars ◽

Significant Difference ◽

Time Required ◽

Vitro Analysis ◽

Cleaning And Disinfection ◽

In Vitro Analysis

Nonsurgical retreatment involves removing mechanical barriers such as gutta-percha to achieve proper cleaning and disinfection. The complexity of the anatomy of molar tooth gives challenge in retreatment procedure. Thus, this study evaluates the amount of residual gutta-percha after retreatment with rotary files (Reciproc Blue®) from each maxillary first molar canal using cone-beam computed tomography (CBCT) and the time required to accomplish it. Nine freshly extracted maxillary molars were instrumented and obturated. Preoperative CBCT was taken, and retreatment was done using Reciproc Blue®. CBCT was taken post retreatment, and the residual volume percentage of gutta-percha from each canal was calculated. The total retreatment time was recorded, and the data were statistically analyzed. The result shows no statistically significant difference in the amount of residual filling material in mesiobuccal, distobuccal, and palatal canal for maxillary first molar and total time used for retreatment with Reciproc Blue® system.

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In vitro analysis of mesenchymal stem cells derived from human teeth and bone marrow

Odontology ◽

10.1007/s10266-012-0075-0 ◽

2012 ◽

Vol 101 (2) ◽

pp. 121-132 ◽

Cited By ~ 67

Author(s):

Yuichi Tamaki ◽

Taka Nakahara ◽

Hiroshi Ishikawa ◽

Soh Sato

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Human Teeth ◽

Vitro Analysis ◽

In Vitro Analysis

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Effect of High Temperature on Composite as Post Endodontic Restoration in Forensic Analysis-An In Vitro Study

Dental Journal of Advance Studies ◽

10.1055/s-0038-1671991 ◽

2014 ◽

Vol 02 (02) ◽

pp. 084-090

Author(s):

Krishma Sharda ◽

Varun Jindal ◽

Ajay Chhabra ◽

◽

Keyword(s):

High Temperatures ◽

Digital Camera ◽

In Vitro Study ◽

Forensic Analysis ◽

Vitro Study ◽

Human Teeth ◽

Great Resistance ◽

Dental Tissues ◽

Different Temperatures

Abstract Aim: The present study was done to evaluate and compare the effect of different temperatures (400 C, 800 C and 1000 C) on Composite as post endodontic restoration using Naked eye, Digital camera and Radiovisiography (RVG) in forensic analysis. Methodology: An in vitro study was conducted on 42 human teeth with composite as post endodontic restoration exposed to three temperature ranges: 400°C, 800°C and 1,000°C. Results: Composite material studied in the present research offered great resistance to high temperatures, without exhibiting considerable macrostructure variation, in such a way that physical changes (dimensional stability, fissures, cracks, fractures, texture, color, carbonization and incineration) can be identified and associated to each specific temperature range. Conclusion: Dental tissues and materials offer great resistance to the effect of high temperatures. Moreover, they present specific changes (color, texture, fissures, cracks fractures, fragmentation) which might contribute to the process of identifying a corpse, or burned, incinerated or carbonized human remains.

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The native structure of the eukaryotic ribosome

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100075890 ◽

1983 ◽

Vol 41 ◽

pp. 432-433

Author(s):

R.A. Milligan ◽

P.N.T. Unwin

Keyword(s):

Ribosomal Proteins ◽

Crystal Form ◽

Native Structure ◽

X Ray Diffraction ◽

Eukaryotic Ribosome ◽

Vitro Analysis ◽

In Vitro Analysis ◽

Resolution Structure ◽

Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

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1163: Radial Dilation of Ureteral Balloons: A Comparative in-Vitro Analysis

The Journal of Urology ◽

10.1016/s0022-5347(18)35308-4 ◽

2005 ◽

Vol 173 (4S) ◽

pp. 315-316

Author(s):

Kari Hendlin ◽

Brynn Lund ◽

Manoj Monga

Keyword(s):

Vitro Analysis ◽

In Vitro Analysis

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An In Vitro Analysis of Ligament Reconstruction or Extension Osteotomy on Trapeziometacarpal Joint Stability and Contact Area

Yearbook of Hand and Upper Limb Surgery ◽

10.1016/s1551-7977(08)70357-1 ◽

2007 ◽

Vol 2007 ◽

pp. 214-215 ◽

Cited By ~ 1

Author(s):

B. Wilhelmi

Keyword(s):

Contact Area ◽

Ligament Reconstruction ◽

Joint Stability ◽

Trapeziometacarpal Joint ◽

Vitro Analysis ◽

In Vitro Analysis

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Role of the 807 C/T Polymorphism of the α2 Gene in Platelet GP Ia Collagen Receptor Expression and Function

Thrombosis and Haemostasis ◽

10.1055/s-0037-1614605 ◽

1999 ◽

Vol 81 (06) ◽

pp. 951-956 ◽

Cited By ~ 59

Author(s):

J. Corral ◽

R. González-Conejero ◽

J. Rivera ◽

F. Ortuño ◽

P. Aparicio ◽

...

Keyword(s):

Venous Thrombosis ◽

Cell Types ◽

Receptor Expression ◽

Case Control Studies ◽

Platelet Surface ◽

Vitro Analysis ◽

And Function ◽

In Vitro Analysis

SummaryThe variability of the platelet GP Ia/IIa density has been associated with the 807 C/T polymorphism (Phe 224) of the GP Ia gene in American Caucasian population. We have investigated the genotype and allelic frequencies of this polymorphism in Spanish Caucasians. The T allele was found in 35% of the 284 blood donors analyzed. We confirmed in 159 healthy subjects a significant association between the 807 C/T polymorphism and the platelet GP Ia density. The T allele correlated with high number of GP Ia molecules on platelet surface. In addition, we observed a similar association of this polymorphism with the expression of this protein in other blood cell types. The platelet responsiveness to collagen was determined by “in vitro” analysis of the platelet activation and aggregation response. We found no significant differences in these functional platelet parameters according to the 807 C/T genotype. Finally, results from 3 case/control studies involving 302 consecutive patients (101 with coronary heart disease, 104 with cerebrovascular disease and 97 with deep venous thrombosis) determined that the 807 C/T polymorphism of the GP Ia gene does not represent a risk factor for arterial or venous thrombosis.

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