scholarly journals Antibody titre avian influenza and Newcastle Disease in blood serum of growing ducks Gived Defferent Crude Protein Ration and vaccinated with Vaksimune NDL AI®

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Purnama Edy Santosa ◽  
Rudy Sutrisna
Keyword(s):  
Avian Influenza ◽  
Blood Serum ◽  
Antibody Titre ◽  
Humoral Immunity ◽  
Newcastle Disease ◽  
Crude Protein ◽  
Risk Of Infection ◽  
Commercial Vaccine ◽  
Antibody Titres ◽  
Genotype Vii

The advantages of vaccination are that it reduces the risk of infection, and concurrently reduces morbidity, mortality and shedding of virus. The goal of the present study was to evaluate efficacy of Newcastle Disease combination with Avian Influenza commercial vaccine based on humoral immunity responses of growing ducks with different feed treatments. Totally, 48 mojosari growing ducks were used in this research. The mojosari growing ducks were vaccinated using Vaksimune NDL AI®. Blood samples were collected from the axilaris vein (left or rigt) one time at postvaccination. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that Vaksimune NDL AI® vaccine inactive ND Genotype VII strain N018 combine with AI subtype H5N1 on emultion oil was a good protection because the vaccine was able to trigger protective humoral immunity of growing ducks at 9 weeks old ducks indicated by increasing of antibody titre in blood serum of vaccinated growing ducks male during three weeks pascavaccination. Key words: Newcastle Disease, Avian influenza, vaccine, antibody, grower ducks

scholarly journals Peningkatan Titer Antibodi Terhadap Avian Influenza Dalam Serum Ayam Petelur yang Divaksin Dengan Vaksin Komersial

2011 ◽  
Vol 11 (1) ◽  
pp. 5-9
Author(s):  
Ummu Balqis ◽  
Muhammad Hambal ◽  
Mulyadi Mulyadi ◽  
Samadi Samadi ◽  
Darmawi Darmawi
Keyword(s):  
Avian Influenza ◽  
Antibody Titre ◽  
Humoral Immunity ◽  
Laying Hens ◽  
Silver Layer ◽  
Risk Of Infection ◽  
Blood Samples ◽  
Commercial Vaccine ◽  
Good Protection ◽  
Antibody Titres

Increasing of antibody titre against avian influenza in serum of vaccinated laying hens with commercial vaccineABSTRACT. The advantages of vaccination are that it reduces the risk of infection, and concurrently reduces morbidity, mortality and shedding of virus. The goal of the present study was to evaluate efficacy of Avian Influenza commercial vaccine based on humoral immunity responses of laying hens. Totally, 20 breakel silver layer hens were used in this research. The laying hens were vaccinated using Avian Influenza commercial vaccine (H5N1). Blood samples were collected from the axilaris vein (left or rigt) one time at prevaccination and repeated three times with an interval of one month at postvaccination. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that Avian Influenza commercial vaccine (H5N1) was a good protection because the vaccine was able to trigger protective humoral immunity of laying hens indicated by increasing of antibody titre in serum of vaccinated laying hens during three months.

scholarly journals Development of BHK-21 Cell line Adapted Inactivated Newcastle Disease Vaccine

2014 ◽  
Vol 3 (1) ◽  
pp. 1-4
Author(s):  
Md Enamul Haque ◽  
Mohammed Ferdousur Rahman Khan ◽  
Marzia Rahman ◽  
Md Tanvir Rahman ◽  
Sukumar Saha ◽  
...  
Keyword(s):  
Cell Line ◽  
Antibody Titre ◽  
Newcastle Disease ◽  
Fusion Gene ◽  
Vaccination Schedule ◽  
Control Group ◽  
Inactivated Vaccine ◽  
Outbreak Area ◽  
Antibody Titres ◽  
And Control

The present study was undertaken to isolate and characterize Newcastle disease virus (NDV) from the outbreak of layer farms for the development of BHK-21 cell adapted inactivated vaccine. A total of 6 dead birds were brought to the laboratory from the outbreak area from which 18 samples (trachea, liver and brain from each) were collected. Among them 3 samples were found positive for NDV through chicken embryo inoculation followed by HA test. The MDT, ICPI, IVPI of all isolates was 54.4, 1.56, and 2.20 respectively which revealed that the field isolates were velogenic. The isolated viruses were confirmed as NDV by reverse transcription-polymerase chain reaction (RT-PCR) using fusion gene-specific primers. The isolated virus was used to infect the BHK-21 cell line. Later the BHK-21 cell adapted viruses were used to develop oil adjuvanted inactivated vaccine and were inoculated into chickens according to vaccination schedule. The MDA was very high (112±29.62) during BCRDV vaccination, which declined quickly (88±33.12). Before vaccination with experimental vaccine, the level of antibody titre (HI) was very low (9±4.65). After vaccination at 65th day through IM, ELD50=109.7/ml with experimental vaccine, the highest HI titre in RDV vaccinated group was 160±59.25 whereas, experimental vaccinated group was 128±59.25, and control group was 7±4.65. ELISA antibody titres of all the groups were 2549.71 (RDV, [email protected] ml/bird), 2450.37 (experimental@ 0.25ml/ bird), 2218.579 (experimental@ 0.50ml/bird), 2152.352 (experimental@1ml/bird) 1125.846 (control) respectively. The present study indicated that, BHK-21 cell adapted ND inactivated vaccine produced a satisfactory antibody titre along with conventional live RDV vaccine.DOI: http://dx.doi.org/10.3329/mh.v3i1.19729 Microbes and Health, June 2014. 3(1): 1-4

Assessment of antibody responses to Newcastle disease vaccination in Nigerian indigenous chicken lines selected for sheep red blood cell antigen

2019 ◽  
Vol 96 (1) ◽  
pp. 47-52
Author(s):  
A.S. Adenaike ◽  
◽  
O.D. Adenaike ◽  
M.A. Opoola ◽  
C.O.N. Ikeobi
Keyword(s):  
Blood Cell ◽  
Antibody Titre ◽  
Red Blood Cell ◽  
Newcastle Disease ◽  
Haemagglutination Inhibition ◽  
Genetic Line ◽  
Chicken Line ◽  
Indigenous Chicken ◽  
Male And Female ◽  
Antibody Titres

This research was carried out to evaluate serum haemagglutination inhibition antibody titres to a Newcastle disease virus vaccine in two Nigerian indigenous chicken lines selected for sheep red blood cell antigens. One hundred Nigerian indigenous chickens were used. These chickens belonged to the fourth generation of flock that had been divergently distinguished into a high antibody titre chicken line (HATC) and a low antibody titre chicken line (LATC). Chicks were given a NDV vaccine at six weeks of age, while blood samples were collected from each chick at seven days post-administration. The antibody titres of chicks were determined through a haemagglutination inhibition test and the data collected were subjected to analysis of variance using SAS 9.2 version. It was found that sex had no significant effect on the antibody titre values (p>0.05), while the genetic line and sex by genetic line interaction had significant effects on the antibody titre values. Female chicks of LATC recorded a significantly lower ND antibody titre (4.21+0.47) compared to HATC females (8.04+2.02). Also, male chicks of LATC recorded a significantly lower ND antibody titre (4.26+1.20) compared to HATC males (7.25+0.48). Meanwhile, there was no significant difference between male and female chicks of LATC as well as between male and female chicks of HATC. Based on the differences observed in ND antibody titres between the genetic lines, emphasis should be placed on policies to develop HATC and LATC along ND resistance. Therefore, we recommend that when considering ND infection which exerts substantial losses to the poultry industry, HATC should be favoured over LATC.

scholarly journals Serological monitoring of avian influenza and Newcastle disease in the Russian Federation in 2019

2020 ◽  
pp. 76-82 ◽  
Author(s):  
M. A. Volkova ◽  
I. A. Chvala ◽  
O. S. Osipova ◽  
M. A. Kulagina ◽  
D. B. Andreychuk ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Blood Serum ◽  
Disease Virus ◽  
Russian Federation ◽  
Newcastle Disease ◽  
Vaccine Antigen ◽  
Reference Laboratory ◽  
Poultry Farms ◽  
The Russian Federation

More than 30,000 samples of blood serum from domestic, wild and synanthropic birds from 50 regions of the Russian Federation were submitted to the FGBI “ARRIAH” (Vladimir) Reference Laboratory for Avian Viral Diseases to be tested for avian influenza and Newcastle disease within the framework of monitoring activities conducted by the Rosselkhoznadzor in 2019. As a result of the laboratory diagnosis, antibodies to type A influenza virus were detected in vaccinated chickens from two poultry farms in the Perm and Primorsky Krais (A/N9). The detected antibodies were specific to the haemagglutinin subtype of the vaccine antigen. As for the backyards in the RF Subjects, where scheduled vaccination against avian influenza A/H5 is carried out, a low level of immunity was seen in the Rostov and Astrakhan Oblasts (35 and 44%, respectively) while a high level of immunity was observed in the Republic of Altai, Krasnodar Krai, the Chechen Republic and the Primorsky Krai (69, 78, 80 and 88%, respectively). High seroprevalence of Newcastle disease virus in adult poultry in indoor holdings was associated with mass vaccination against the disease. In broiler chickens, post-vaccination antibodies were observed, on average, in 42% of the studied blood serum samples. Antibodies to the Newcastle disease virus were detected in 39% of samples from backyard chickens. Seroprevalence in wild and synanthropic birds was high. The obtained results suggest that the risk of introduction and spread of avian influenza and Newcastle disease in industrial poultry farms and in backyards remains.

scholarly journals Viral antibodies in south polar skuas around Davis Station, Antarctica

2008 ◽  
Vol 20 (5) ◽  
pp. 455-461 ◽  
Author(s):  
Gary D. Miller ◽  
Joanne M. Watts ◽  
Geoffrey R. Shellam
Keyword(s):  
Avian Influenza ◽  
Blood Serum ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Viral Antibodies ◽  
Bursal Disease ◽  
Catharacta Maccormicki ◽  
South Polar

AbstractWe surveyed south polar skuas (Catharacta maccormickiSaunders) in Antarctica for antibodies to a series of viral agents using blood serum and cloacal swabs from 124 adult skuas. There were no ticks on the subjects and we were unable to isolate virus from any individual. Skuas, however, were seropositive to some avian viruses: 16.9% (20/118) had antibodies to infectious bursal disease virus and 10.5% (11/105) were seropositive for Newcastle disease. We found 1.0% (1/98) had antibodies to avian influenza, no evidence of egg drop syndrome (0/48), but 27.8% (10/36) had antibodies to flaviviruses. Clearly south polar skuas encounter a variety of pathogens either in Antarctica or during their migration in the non-breeding season. There was no evidence of disease in skuas, but the presence of antibodies to pathogens indicates the need to continue to search for the origins of these immunological challenges.

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