scholarly journals Development of BHK-21 Cell line Adapted Inactivated Newcastle Disease Vaccine

2014 ◽  
Vol 3 (1) ◽  
pp. 1-4
Author(s):  
Md Enamul Haque ◽  
Mohammed Ferdousur Rahman Khan ◽  
Marzia Rahman ◽  
Md Tanvir Rahman ◽  
Sukumar Saha ◽  
...  
Keyword(s):  
Cell Line ◽  
Antibody Titre ◽  
Newcastle Disease ◽  
Fusion Gene ◽  
Vaccination Schedule ◽  
Control Group ◽  
Inactivated Vaccine ◽  
Outbreak Area ◽  
Antibody Titres ◽  
And Control

The present study was undertaken to isolate and characterize Newcastle disease virus (NDV) from the outbreak of layer farms for the development of BHK-21 cell adapted inactivated vaccine. A total of 6 dead birds were brought to the laboratory from the outbreak area from which 18 samples (trachea, liver and brain from each) were collected. Among them 3 samples were found positive for NDV through chicken embryo inoculation followed by HA test. The MDT, ICPI, IVPI of all isolates was 54.4, 1.56, and 2.20 respectively which revealed that the field isolates were velogenic. The isolated viruses were confirmed as NDV by reverse transcription-polymerase chain reaction (RT-PCR) using fusion gene-specific primers. The isolated virus was used to infect the BHK-21 cell line. Later the BHK-21 cell adapted viruses were used to develop oil adjuvanted inactivated vaccine and were inoculated into chickens according to vaccination schedule. The MDA was very high (112±29.62) during BCRDV vaccination, which declined quickly (88±33.12). Before vaccination with experimental vaccine, the level of antibody titre (HI) was very low (9±4.65). After vaccination at 65th day through IM, ELD50=109.7/ml with experimental vaccine, the highest HI titre in RDV vaccinated group was 160±59.25 whereas, experimental vaccinated group was 128±59.25, and control group was 7±4.65. ELISA antibody titres of all the groups were 2549.71 (RDV, [email protected] ml/bird), 2450.37 (experimental@ 0.25ml/ bird), 2218.579 (experimental@ 0.50ml/bird), 2152.352 (experimental@1ml/bird) 1125.846 (control) respectively. The present study indicated that, BHK-21 cell adapted ND inactivated vaccine produced a satisfactory antibody titre along with conventional live RDV vaccine.DOI: http://dx.doi.org/10.3329/mh.v3i1.19729 Microbes and Health, June 2014. 3(1): 1-4

scholarly journals Antibody responses in buffalos immunized with Clostridium perfringens beta and epsilon toxoids

2001 ◽  
Vol 46 (No. 9–10) ◽  
pp. 241-243 ◽  
Author(s):  
S. Rahman M ◽  
K. Baek B ◽  
T. Hong S ◽  
H. Lee J
Keyword(s):  
Antibody Titre ◽  
Clostridium Perfringens ◽  
Neutralizing Antibody ◽  
Antibody Responses ◽  
Control Group ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Antibody Titres ◽  
Group A ◽  
And Control

The antibody responses to toxoids were measured to investigate whether Clostridium perfringens beta and epsilon toxoids induced protective humoral immune responses in buffalos. Total of 24 buffalos were divided into 4 groups (n = 6), beta toxoid, epsilon toxoid, combination and control groups. These buffalo groups were administered each of the designated toxoids. Immunizations in the beta and epsilon toxoid groups induced strong antibody responses. The neutralizing antibody titres from the beta and epsilon toxoid groups were equally log101.2 on day 21 after inoculation whereas there was no antibody titre detected from the control group. A statistically significant (P < 0.01) increase in antibody titre was observed from day 0 to day 14 and 21 after inoculation. The antibody production did not vary significantly due to day of inoculation and toxoid interactions.

scholarly journals Antibody titre avian influenza and Newcastle Disease in blood serum of growing ducks Gived Defferent Crude Protein Ration and vaccinated with Vaksimune NDL AI®

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Purnama Edy Santosa ◽  
Rudy Sutrisna
Keyword(s):  
Avian Influenza ◽  
Blood Serum ◽  
Antibody Titre ◽  
Humoral Immunity ◽  
Newcastle Disease ◽  
Crude Protein ◽  
Risk Of Infection ◽  
Commercial Vaccine ◽  
Antibody Titres ◽  
Genotype Vii

The advantages of vaccination are that it reduces the risk of infection, and concurrently reduces morbidity, mortality and shedding of virus. The goal of the present study was to evaluate efficacy of Newcastle Disease combination with Avian Influenza commercial vaccine based on humoral immunity responses of growing ducks with different feed treatments. Totally, 48 mojosari growing ducks were used in this research. The mojosari growing ducks were vaccinated using Vaksimune NDL AI®. Blood samples were collected from the axilaris vein (left or rigt) one time at postvaccination. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that Vaksimune NDL AI® vaccine inactive ND Genotype VII strain N018 combine with AI subtype H5N1 on emultion oil was a good protection because the vaccine was able to trigger protective humoral immunity of growing ducks at 9 weeks old ducks indicated by increasing of antibody titre in blood serum of vaccinated growing ducks male during three weeks pascavaccination. Key words: Newcastle Disease, Avian influenza, vaccine, antibody, grower ducks

scholarly journals Effect of vitamins, minerals and probiotics on production of antibody and live weight gain following vaccination with BCRDV in broiler birds

1970 ◽  
Vol 6 (1) ◽  
pp. 31-36 ◽  
Author(s):  
P Monoura ◽  
M Rahman ◽  
MFR Khan ◽  
MB Rahman ◽  
MM Rahman
Keyword(s):  
Antibody Titre ◽  
Serum Antibody ◽  
Live Weight ◽  
Poultry Feed ◽  
Control Group ◽  
Broiler Chicks ◽  
Antibody Titres ◽  
Group A ◽  
Group B ◽  
Group D

The effect of vitamins, minerals and probiotics supplementation to the balanced ration of the broiler chicks on the immune response and growth performance after vaccination with BCRDV was evaluated. For this 50 broiler birds were divided into five equal groups such as A, B, C, D and E, and five types of separate treatments with normal poultry feed were provided to the indivisual experimental groups. All the birds were vaccinated with BCRDV primarily at 3 days of age and boostered at 17 days of age. The birds of group A, B, C and D were supplemented with only probiotics, mixture of probiotics and minerals, mixture of probiotics, minerals and vitamins, respectively. The birds of group E were kept as vaccinated control. Blood samples were collected at the age of 9, 12, 16, 23, 26 and 30 days sequencially. The serum antibody titres were measured by HI test and ELISA. The highest value of HI antibody titre was 384±242.65 (Mean ±SD) in group B at 23 days of age. The lowest value of HI at 23 days was 56±32.86 (Mean ±SD) obtained in group C. The ELISA antibody titre was also highest (4675.12±485.72) at 23 days in group B and obviously the lowest (2169.38±724.45) in control group E. In case of group C, the ELISA antibody titre was 3105.73±877.57 at 23 days but then the titre decreased gradually compared to control group. The highest body weight (1420±90.82) was recorded at 30 days of age in group D and the lowest value (1188±44.38) was in group B. Key words: Probiotics, BCRDV, broiler birds DOI = 10.3329/bjvm.v6i1.1336 Bangl. J. Vet. Med. (2008). 6 (1): 31-36

Assessment of antibody responses to Newcastle disease vaccination in Nigerian indigenous chicken lines selected for sheep red blood cell antigen

2019 ◽  
Vol 96 (1) ◽  
pp. 47-52
Author(s):  
A.S. Adenaike ◽  
◽  
O.D. Adenaike ◽  
M.A. Opoola ◽  
C.O.N. Ikeobi
Keyword(s):  
Blood Cell ◽  
Antibody Titre ◽  
Red Blood Cell ◽  
Newcastle Disease ◽  
Haemagglutination Inhibition ◽  
Genetic Line ◽  
Chicken Line ◽  
Indigenous Chicken ◽  
Male And Female ◽  
Antibody Titres

This research was carried out to evaluate serum haemagglutination inhibition antibody titres to a Newcastle disease virus vaccine in two Nigerian indigenous chicken lines selected for sheep red blood cell antigens. One hundred Nigerian indigenous chickens were used. These chickens belonged to the fourth generation of flock that had been divergently distinguished into a high antibody titre chicken line (HATC) and a low antibody titre chicken line (LATC). Chicks were given a NDV vaccine at six weeks of age, while blood samples were collected from each chick at seven days post-administration. The antibody titres of chicks were determined through a haemagglutination inhibition test and the data collected were subjected to analysis of variance using SAS 9.2 version. It was found that sex had no significant effect on the antibody titre values (p>0.05), while the genetic line and sex by genetic line interaction had significant effects on the antibody titre values. Female chicks of LATC recorded a significantly lower ND antibody titre (4.21+0.47) compared to HATC females (8.04+2.02). Also, male chicks of LATC recorded a significantly lower ND antibody titre (4.26+1.20) compared to HATC males (7.25+0.48). Meanwhile, there was no significant difference between male and female chicks of LATC as well as between male and female chicks of HATC. Based on the differences observed in ND antibody titres between the genetic lines, emphasis should be placed on policies to develop HATC and LATC along ND resistance. Therefore, we recommend that when considering ND infection which exerts substantial losses to the poultry industry, HATC should be favoured over LATC.

scholarly journals Efficacy study of bio-typhoid® vaccine against fowl typhoid in backyard layer chicken

1970 ◽  
Vol 7 (2) ◽  
pp. 295-299
Author(s):  
MZ Uddin ◽  
MSR Khan ◽  
F Begum ◽  
MA Mannan
Keyword(s):  
Antibody Titre ◽  
Virulent Strain ◽  
Primary Vaccination ◽  
Control Group ◽  
Fowl Typhoid ◽  
Layer Chicken ◽  
Unvaccinated Control ◽  
Antibody Titres ◽  
Group A ◽  
Group B

The study was performed with a view to isolate and identify a virulent strain of S. gallinarum and determine the purity, safety and efficacy of BIO-TYPHOID® vaccine. A total of 40 backyard layer chicken were used for this study where Group A was used for experimental vaccination, Group B kept as control and Group C was used for calculating virulent S. gallinarum challenge dose. Primary and secondary vaccination was carried out at 40 days and 110 days of age, respectively. Blood samples were collected to obtain the sera after vaccination from both vaccinated and unvaccinated control group and antibody titres were determined by Microplate agglutination test. The antibody titre increased in primary vaccination up to days 56 days post vaccination (DPV) and then decreased gradually. The highest antibody titre (Mean ± SE) 384.00 ± 42.67 was obtained at 91 DPV (21 days later of secondary vaccination) and maintained up to 98 DPV. Safety test was done by inoculating mice and purity test of the vaccine was done by inoculating on to Blood Agar media. The efficacy of BIO-TYPHOID® vaccine was recorded as 90% which was determined by challenge infection with 0.1 ml of 5x105 CFU virulent S. gallinarum. Results of this study revealed successful protections by BIO-TYPHOID® vaccine. Keywords: BIO-TYPHOID® vaccine; Chicken; Efficacy; Fowl Typhoid DOI: 10.3329/jbau.v7i2.4737 J. Bangladesh Agril. Univ. 7(2): 295-299, 2009

EFFECT ON IMMUNE RESPONSE AND VIRUS SHEDDING IN THE CHICKEN VACCINATED AGAINST INACTIVATED LOCAL STRAIN OF NEWCASTLE DISEASE VIRUS GENOTYPE VII

2015 ◽  
Vol 77 (25) ◽  
Author(s):  
Nursyuwari Nayan ◽  
Syamsiah Aini Shohaimi ◽  
Raha Ahmad Raus ◽  
Afzan Mat Yusof ◽  
Ong Geok Huai
Keyword(s):  
Immune Response ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Local Strain ◽  
Control Group ◽  
Inactivated Vaccine ◽  
Virus Shedding ◽  
Oil Emulsion ◽  
Genotype Vii

The effectiveness of the new inactivated vaccine develop from local velogenic strain of Newcastle disease virus (NDV) Genotype VII and commercial vaccines LaSota were compared by determining the immune response and virus shedding of vaccinated chickens. Ten different groups of chicken consists of ten each, were vaccinated with and without adjuvant of the inactivated vaccine via intramuscular and subcutaneous, respectively. Three different adjuvants were used which include Oil-emulsion Complete Freund’s, Oil-emulsion Squalene-based, and aluminium hydroxide gel (Alum). As a comparison, a group of chicken was vaccinated with commercial vaccine and control group was not vaccinated. With 100% survival rate and highest mean haemaglutination-inhibition (HI) titre of log2 6, the inactivated vaccine with adjuvant Alum and Oil-emulsion Complete Freund’s surpassed the LaSota. In addition, the virus shedding was significantly reduced and comparable to LaSota vaccinated chicken. Whereas, without adjuvant, the chicken HI antibody titre is below log2 4 after vaccination and only 20-30% were survived. Based on the post-mortem findings on the survived chicken from each vaccinated group, their internal organs appeared normal and no sign of haemorrhage or pathognomonic signs of Newcastle disease (ND). Conclusively, vaccinated chicken are effectively protected from morbidity and mortality against virulent genotype VII challenge with the addition of adjuvant into inactivated local strain of NDV genotype VII vaccine. Thus, the development of inactivated local NDV genotype VII vaccine is a promising candidate to control the current ND endemic in Malaysia. 

scholarly journals Effect of injection hatching eggs with Newcastle disease vaccine and different doses of vitamin E on some productive traits and immune response of broilers

2015 ◽  
Vol 39 (2) ◽  
pp. 98-107
Author(s):  
Mushtaq T. Abdulwahid
Keyword(s):  
Immune Response ◽  
Vitamin E ◽  
Newcastle Disease ◽  
Control Group ◽  
Feed Conversion ◽  
Productive Traits ◽  
Hatching Eggs ◽  
And Control ◽  
Newcastle Disease Vaccine ◽  
Different Doses

     This study aimed to investigate the effects of injection hatching eggs with different doses of vitamin E on productive traits, some physiological and immune response to Newcastle disease vaccine. Two hundred fertile eggs of broiler breeder Ross (308) strain were incubated in automatic incubator machine after divided into four treatments (50 eggs per treatment) with two replicates. First treatment was injected 0.1ml/egg of phosphate buffer sterile into amniotic fluid at day 18th of incubation which it was considered as control group, second treatment was injected with 0.1ml/egg of inactivated Newcastle disease vaccine, third treatment was injected with 0.1ml/egg of inactivated Newcastle disease vaccine and 0.1 ml/egg of vitamin E, fourth treatment was injected with 0.1ml/egg of inactivated Newcastle disease vaccine and 0.15 ml/egg of vitamin E. All injected eggs were carried back into incubator for complete hatching process. Hatched chicks were transferred to the hall in farm of the Veterinary Medicine College/University of Baghdad; therefore, the chicks were distributed into four treatments with two replicates depending on the previously treated groups until fifth week of age. The results revealed that the treated groups with vitamin E were significantly (P≤0.05) increased in hatchability percentage, body weight, weight gain, as well as significant reduce in feed intake and improvement in feed conversion ratio as compared with second group and control, the results of third group showed significantly increased (P≤0.05) in antibody titers against Newcastle disease virus at aged (21 and 35) day as compared with the other treated groups and control.

scholarly journals Passive immunoprophylaxis of edema disease in weaned piglets

2012 ◽  
Vol 49 (No. 12) ◽  
pp. 447-452 ◽  
Author(s):  
P. Alexa ◽  
J. Hamrik ◽  
K. Stouracova ◽  
L. Konstantinova ◽  
E. Salajka
Keyword(s):  
Neutralizing Antibodies ◽  
Clinical Manifestations ◽  
Immune Serum ◽  
Control Group ◽  
Edema Disease ◽  
Porcine Serum ◽  
Antibody Titres ◽  
Slaughter Pigs ◽  
And Control ◽  
Free Serum

Hyperimmune sera against Shiga toxin Stx2e with antibody titres 8 000–32 000, prepared by immunization of slaughter pigs, were administered subcutaneously to piglets (n = 73) 3 days after weaning. The titres of Stx2e neutralizing antibodies in sera of piglets ranged from 16 to 512 following application of the immune serum. In most of the piglets, passively acquired antibodies disappeared during 4 to 8 weeks after application. The recorded serum titres were in all of the piglets 8 or higher fourteen days following administration. In another experiment, an immune porcine serum with antibody titre against Stx2e of 130 000 was applied subcutaneously into a knee fold to 30 piglets. The serum was applied at the dose 3 ml, 1.5 ml, 0.75 ml, 0.4 ml, 0.2 ml, 0.1 ml, and 0.05 ml based on the group of piglets. Antibody-free serum was applied to pigs of the control group (n = 5). The following day, supernatant of STEC culture with Stx2e titre of 150 000 was applied to piglets of both experimental and control groups into the opposite knee fold that the above serum. Piglets that received 0.2 to 3 ml of serum remained unaffected throughout the experiment following application of supernatant containing Stx2e. Piglets that received 0.05 ml of serum and those of the control group showed severe clinical manifestations of edema disease following Stx2e application and were sacrificed ante finem. Marked signs of the disease could already be observed 18 hrs following Stx2e application. In a group of 3 piglets that received 0.1 ml of serum, mild paralysis was observed in 1 piglet 18 hrs following Stx2e application.

scholarly journals Scoring System for Lesions Induced by Different Strains of Newcastle Disease Virus in Chicken

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Elawad A. Hussein ◽  
M. Hair-Bejo ◽  
Lawan Adamu ◽  
A. R. Omar ◽  
Siti S. Arshad ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Scoring System ◽  
Control Group ◽  
Control Groups ◽  
Specific Pathogen ◽  
Group 5 ◽  
Different Strains ◽  
And Control

Newcastle disease virus strains are velogenic, mesogenic, and lentogenic. This study aims to design a scoring system for lesions induced by different strains of Newcastle disease virus in chicken. Three experiments were conducted. In experiments 1 and 2, chickens were divided into infected and control groups. Infected groups of experiments 1 and 2 consisted of 6 and 24 specific pathogen-free (SPF) chickens, respectively. Control groups in experiments 1 and 2 consisted of 6 and 15 SPF chickens, respectively. In infected groups, infection was induced by intranasal administration of 10550% EID50/0.1 mL of velogenic Newcastle disease virus strain (vNDV). Infected chickens in experiment 1 were euthanised by cervical dislocation on days 3, 6, and 7 postinoculation (pi). Infected chickens in experiment 2 were euthanised at hours (hrs) 2, 4, 6, 12 and days 1, 2, 4, and 6 pi. Chickens of the control group in experiment 1 were euthanised on days 3 and 7 pi, whereas control group chickens in experiment 2 were euthanised on days 0, 1, 2, 4, and 6 pi. Then in experiment 3, 15 SPF chickens were divided into three groups; in the first group, 5 SPF chickens were infected with vNDV, in the second group, 5 SPF chickens were infected with lentogenic NDV (lNDV) (103.0EID50/0.1 mL), and the third group was kept without infection as a control group. Chickens were euthanised on day 5 pi. In all previous experiments, tissues of brain, trachea, lung, caecal tonsil, liver, kidney, spleen, heart, proventriculus, intestine, and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin, and sectioned. HS staining was applied. Tissues were examined under light microscope and changes were recorded. A scoring system was designed for lesions induced by different strains of NDV and, accordingly, lesions were scored. The scoring system was found helpful in the evaluation of disease severity.

scholarly journals The prevalence of bovine viral diarrhoea antibodies in selected South African dairy herds, and control of the disease

2000 ◽  
Vol 71 (1) ◽  
Author(s):  
G.M. Ferreira ◽  
D.C. Lourens ◽  
M. Van Vuuren
Keyword(s):  
South African ◽  
Antibody Titre ◽  
Control Measures ◽  
Dairy Herds ◽  
Inactivated Vaccine ◽  
Serological Testing ◽  
Bovine Viral Diarrhoea ◽  
Persistently Infected ◽  
Pathological Lesions ◽  
And Control

The prevalence of bovine viral diarrhoea (BVD) serologically positive animals in 18 dairy herds with clinical and pathological lesions suggestive of BVD infection, the post-vaccinal seroconversion rates in negative animals vaccinated twice with an inactivated BVD vaccine, and the control measures taken, are described. The pathological and histopathological findings in 6 necropsies performed on animals that died in 5 separate herds closely resembled published descriptions. Positive immunohistochemistry results in 3 cases confirmed the diagnosis in those animals. In 1 herd the prevalence of prevaccinal BVDantibodies was only 36.8 %, while the prevalence varied from 79.85 to 100 % in the remainder. Control measures taken included immunoprophylaxis with an inactivated vaccine, culling animals that were serologically negative after vaccination that were regarded as probably persistently infected (PI) and the implementation of additional biosecurity measures. The prevalence of serologically negative PI animals in 10 herds varied from 0.38 to 4.04 %, with 8 herds less than 1 %and 2 herds at 2.79 %and 4.04 %, respectively. Methods based on vaccinating the herd, followed by serological testing and culling cattle that did not develop an antibody titre, are not reliable. The identification of PI animals should be confirmed by isolation of the virus or identification of the antigen.

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