scholarly journals THE TESTING OF SSR MARKERS OF GENES CONTROLLING RESISTANCE TO P. HALSTEDII AND THE SELECTION OF OPTIMAL CONDITIONS FOR PCR

2021 ◽  
Author(s):  
E.V. Badyanov ◽  
◽  
S.A. Ramazanova ◽  
Keyword(s):  
Dna Markers ◽  
Optimal Conditions ◽  
Optimal Temperature ◽  
Optimal Method ◽  
Allelic Variants ◽  
Effective Measure ◽  
Temperature Regimes ◽  
Resistant Varieties ◽  
Dna Genotyping ◽  
Selection Of

Downy mildew is one of the most harmful diseases of sunflower. The most effective measure of this disease control is the development of resistant varieties and hybrids. The use of molecular markers, in particular DNA markers, allows to control the presence of dominant resistance genes at each stage of breeding. We carried out the selection of the optimal method for the isolation of sunflower DNA and the selection of the optimal temperature regimes of amplification for 10 pairs of primers developed to mark the Pl6, Pl8, Pl13, and PlArg genes. Preliminary, we identified 13 allelic variants that are suitable for DNA genotyping of Helianthus annuus by these loci.

ANALYSIS OF GENETIC STRUCTURE OF DOGS OF FRENCH BULLDOG BREED USING MICROSATELLITE DNA MARKERS

2018 ◽  
Vol 51 ◽  
pp. 185-192
Author(s):  
S. Kruhlyk ◽  
V. Dzitsiuk ◽  
V. Spyrydonov
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
High Variability ◽  
Molecular Diagnostic ◽  
Allelic Variants ◽  
Microsatellite Dna Markers ◽  
Gene Complexes ◽  
French Bulldog

Genetic variability of domestic dogs is a source for effective process of breed formation and creating unique gene complexes. In the world, for preservation of genetic resources of dogs, there are dog training associations which have great confidence: American Club Dog Breeders (AKC), the British Kennel Club (KC) and the Federation Cynologique Internationale (FCI), aimed at protecting breeding dogs, standards creation, registration of a breed, and issuance of accurate pedigrees. Evaluation of the genetic diversity of dog breeds is able to significantly complement and improve their breeding programs. Since breeds of dog differ in morphological and economic characteristics, the problem of finding of the breed features in the genome of animals is becoming more topical. From this point of view, French Bulldog is an interesting breed of dog (FRANC.BULLDOGGE, FCI standard number 101) which belongs by the classification of breeds, adopted in FCI, to the group IX – a dog-companion for health and fun, but to a subgroup of fighting dogs of a small format. French Bulldog breed has been researched slightly not only in Ukraine and also abroad, as the main work of all dog association is focused on solving theoretical and practical issues of breeding, keeping, feeding, veterinary protection and others. The study was conducted at Research Department of Molecular Diagnostic Tests of Ukrainian Laboratory of Quality and Safety of Agricultural Products. 33 animals of French Bulldog breed, admitted to use in dog breeding of Ukrainian Kennel Union (UKU), were involved for the genetic analysis using DNA markers. The materials of the research were buccal epithelial cells, selected before the morning feeding of animals by scraping mucous membrane of oral cavity with disposable, dry, sterile cotton swab. Genomic DNA was extracted using KIT-set of reagents for DNA isolation according to the manufacturer's instructions. PEZ1, PEZ3, PEZ6, PEZ8, FHC 2010, FHC 2054 markers, recommended by International Society for Animal Genetics (ISAG), ACN, КC and FCI, were used for research. As a result of research 25 alleles for all the loci were detected in the experimental sample of dogs. The average number of alleles at the locus Na, obtained by direct counting, was 4.16. The most polymorphic loci for this breed were PEZ6 and PEZ3 with 8 and 6 allelic variants. Monomorphic loci were PEZ8 and FHC 2054 which had 4 and 3 alleles and the lowest level of polymorphism was observed for PEZ 1 and FHC 2010 loci in which only 2 alleles were identified. On analyzing the molecular genetic characteristics of dogs of French Bulldog breed, we found a high variability of genotype on rare alleles, which included alleles: M, C, D, E, J, K, L, O, N and representing 60% of the total number of the identified alleles. C, D, E alleles for PEZ3 locus and O allele at PEZ6 locus are unique to the sampling of dogs because they are not repeated in other loci. Typical alleles: N, F, R, I, P, K, M are 40% of the total. But F, R alleles for PEZ3 locus and P allele for locus PEZ6 are not repeated either in standard allelic variants or in rare one, indicating a high information content of these alleles and loci to be used for further monitoring of allele pool, genetic certification and identification of dogs. Microsatellite DNA loci were analyzed as a result of investigations of French Bulldogs and the most informative: PEZ3, PEZ6 and PEZ8 were found, which have high efficiency in individual and breed certification of dogs due to high variability. These data allow further monitoring of the state of genetic diversity of the breed and the development of measures for improvement of breeding to preserve the structure of breeding material. The study of individual and population genetic variability is advisable to continue for breeding of French Bulldogs "in purity" and preserving valuable gene complexes. The results are the basis for further monitoring of the proposed informative panels of microsatellite DNA markers for genotyping dog of French Bulldog breed and their complex evaluation.

Approaches to the Organization of International Accounting in Russian Enterprises

TEM Journal ◽  
2021 ◽  
pp. 249-258
Author(s):  
Ekaterina V. Surkova ◽  
Galina A. Skachko ◽  
Larisa K. Nikandrova ◽  
Maria M. Starkova ◽  
Nina F. Sakharova
Keyword(s):  
Financial Reporting ◽  
Accounting Information ◽  
Financial Statements ◽  
Reporting Standards ◽  
Optimal Method ◽  
Accounting Standard ◽  
Financial Reporting Standards ◽  
Advantages And Disadvantages ◽  
The Individual ◽  
Selection Of

The article discusses current issues of transformation of accounting information in accordance with international financial reporting standards (IFRS). This study is primarily aimed at developing approaches that determine the need for Russian enterprises to provide accounting information comparable at the international level. The authors analyze methods of transferring data from the Russian Accounting Standard (RAS) to IFRS. The methods used to form financial statements in accordance with IFRS are discussed. The issues of the application of these methods, as well as their advantages and disadvantages, are discussed. The author's approach to the selection of the optimal method of transformation is proposed taking into account the individual needs of organizations.

scholarly journals ANALYSIS OF EXTERNAL ENGINEERING NETWORK METHODS

2020 ◽  
Vol 4 (157) ◽  
pp. 7-11
Author(s):  
V. Zhvan ◽  
V. Donenko ◽  
S. Kulish ◽  
A. Taran
Keyword(s):  
Physical And Mechanical Properties ◽  
Directional Drilling ◽  
Horizontal Directional Drilling ◽  
Technological Parameters ◽  
Optimal Method ◽  
Advantages And Disadvantages ◽  
Analytical Review ◽  
Conducting Research ◽  
The Cost ◽  
Selection Of

The article is devoted to the effective analysis of trench and trenchless pipeline laying technologies. In the course of the work, an analytical review of pipeline assembly was performed, the main technological parameters, the scope of each method, and their advantages and disadvantages were determined. List of considered pipeline laying methods: trenching, horizontal directional drilling, mechanical puncture, hydraulic puncture, microtunneling and punching. The article analyzes the classical trench method and the most widely used trenchless ones: horizontal directional drilling; mechanical puncture; hydraulic puncture; microtunneling; punching. Each of these methods has several advantages and disadvantages. The choice of the optimal method of laying the pipeline depends on many factors: the physical and mechanical properties of soils and hydrogeological conditions, the length and diameter of the pipeline, the presence of other communications, buildings and structures, as well as the budget that customers have. Work time is the last deciding factor. Based on the results of the analysis of pipeline laying technologies and expert survey of construction industry experts, the cost table of each method was compiled, outlining the main characteristics of the technology: length of pipeline, speed of work, scope, cost, and the advantages and disadvantages of each of the considered methods. The conclusions about the use of each of the pipeline laying methods were made. Each of the methods has its advantages and disadvantages, so to choose the method of work it is necessary to conduct a comprehensive assessment of technological parameters, cost, scope and timing of work. The cost of lay-ing the pipeline consists of the following factors: conducting research; selection of diameter and determination of pipeline length; choice of laying method and equipment necessary for the works; selection of equipment, shut-off and control equipment and other materials arranged on the pipeline; terms of performance of works. Taking into account these factors, an estimate is made, which determines the cost of installation of a particular pipeline. After the analysis, we can conclude that among the methods of trenchless laying of pipelines can be identi-fied horizontally directional drilling, it is this method of laying the pipeline will be appropriate to use for our region. The drilling technique allows to carry out pipelines under obstacles, to pull long segments of networks, to repair site damage. This method is universal and can be used in almost any environment. Keywords: trenches, horizontal directional drilling, mechanical puncture, hydraulic piercing, microtunnelling, punching, pipeline.

scholarly journals Herbicide-resistant crops in resistant weed management : An industrial perspective

2005 ◽  
Vol 75 (4) ◽  
pp. 79-84 ◽  
Author(s):  
D. Shaner
Keyword(s):  
Weed Control ◽  
Weed Management ◽  
Wild Species ◽  
Integrated Weed Management ◽  
Herbicide Resistant ◽  
Resistant Varieties ◽  
Selection Of

Some of the first products of biotechnology to reach the marketplace have been herbicide-resistant crops. Industry sees the development of herbicide-resistant varieties as a way to increase the availability of proven herbicides for a broader range of crops. However, the development of herbicide- resistant crops requires special attention to potential environmental questions such as herbicide usage, selection of resistant weed biotypes and spread of resistance from the resistant crop to wild species. Industry is actively addressing these concerns during the process of development. Proper development and use of herbicide-resistant crops in integrated weed management programs will provide farmers with increased flexibility, efficiency, and decreased cost in their weed control practices without increasing the risk of herbicide-resistant weeds. Furthermore, herbicide-resistant crops should prove to be valuable tools in managing herbicide- resistant weeds.

POLYMORPHISM OF PROLACTIN AND SOMATOTROPIN GENES IN DAIRY CATTLE OF THE REPUBLIC OF TATARSTAN

2021 ◽  
pp. 22-25
Author(s):  
Р.Р. ШАЙДУЛЛИН ◽  
А.Б. МОСКВИЧЕВА ◽  
Т.Х. ФАИЗОВ ◽  
Л.Р. ЗАГИДУЛЛИН ◽  
Т.М. АХМЕТОВ ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Dna Markers ◽  
Frequency Of Occurrence ◽  
Allelic Variants ◽  
Black And White ◽  
Prolactin Gene ◽  
White Breed ◽  
White Cattle ◽  
Cattle Herds ◽  
The Republic

Изучен полиморфизм и определена частота встречаемости аллельных вариантов по генам пролактина (PRL) и соматотропина (GH) у коров в условиях двух племенных хозяйств Республики Татарстан. Объектом исследования были животные черно-пестрой породы первого (n=151) и третьего (n=168) отелов и холмогорской породы первого (n=160) и третьего (n=143) отелов. Проведено генотипирование коров по генам PRL и GH методом ДНК-диагностики. Установлено, что среди молочного скота преимущественно встречаются особи с генотипом PRL АА (70,0—77,5%), с частотой аллеля А гена пролактина — 0,83—0,87, аллеля В — 0,13—0,17. Встречаемость аллеля В была выше среди холмогорских коров (0,16—0,17). В стаде полновозрастных животных черно-пестрой породы имеется сдвиг генетического равновесия в сторону генотипов PRL AA и PRL BB (χ2=3,97; Р<0,05). В исследуемых стадах крупного рогатого скота выражено преимущество генотипа GH LL с частотой встречаемости 51,0—79,2%. Встречаемость генотипа GH VV у черно-пестрого скота составила 2,0—2,4%, у холмогорского — 5,6—7,0%. Аллель L гена соматотропина преобладает над аллелем V в обеих породах. При этом чаще встречаемость аллеля L GH среди коров холмогорской породы (0,28—0,29). У холмогорских коров больше представлены редкие и желательные аллели генов PRL и GH. Чтобы повысить встречаемость «предпочтительных» в хозяйственном отношении генотипов и аллелей ДНК-маркеров, необходимо в большей степени использовать быков-производителей с желательными аллелями PRL и GH, а именно В и V соответственно. The polymorphism was studied and the frequency of occurrence of allelic variants for the genes of prolactin (PRL) and somatotropin (GH) was determinedin cows in two bred livestock farms of the Republic of Tatarstan. The object of the study were animals of the black-and-white breed of the first (n=151) and third (n=168) calving and the Kholmogory breed of the first (n=160) and third (n=143) calving. Genotyping of cows for PRL and GH genes was carried out using method of DNA diagnostics. It was revealed that there were mainly individuals with the PRL AA genotype (70,0–77,5%)among dairy cattle, the frequency of the A allele of the prolactin gene was 0,83–0,87, the B allele was 0,13–0,17. The frequency of allele B was higher among Kholmogory cows (0,16–0,17). There was a shift in the genetic balance towards the PRL AA and PRL BB genotypes (χ2=3,97; Р<0,05) in the herd of full-age animals of the black-and-white breed. The advantage of the GH LL genotype was expressed with a frequency of occurrence 51,0–79,2% in the studied cattle herds. The occurence of the GH VV genotype was 2,0–2,4%in black-and-white cattle, 5,6–7,0% was in Kholmogorycattle. The L allele of the somatotropin gene predominated over the V allele in both breeds. Moreover, the occurrence of the L GH allele among cows of the Kholmogory breed (0,28–0,29) was more often. Rare and desirable alleles of the PRL and GH genes are more represented in Kholmogory cows. In order to increase the occurrence of economically “preferred” genotypes and alleles of DNA markers, it is necessary to use more stud bulls with the desired PRL and GH alleles, namely B and V, respectively.

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