POLYMORPHISM OF PROLACTIN AND SOMATOTROPIN GENES IN DAIRY CATTLE OF THE REPUBLIC OF TATARSTAN

Author(s):  
Р.Р. ШАЙДУЛЛИН ◽  
А.Б. МОСКВИЧЕВА ◽  
Т.Х. ФАИЗОВ ◽  
Л.Р. ЗАГИДУЛЛИН ◽  
Т.М. АХМЕТОВ ◽  
...  

Изучен полиморфизм и определена частота встречаемости аллельных вариантов по генам пролактина (PRL) и соматотропина (GH) у коров в условиях двух племенных хозяйств Республики Татарстан. Объектом исследования были животные черно-пестрой породы первого (n=151) и третьего (n=168) отелов и холмогорской породы первого (n=160) и третьего (n=143) отелов. Проведено генотипирование коров по генам PRL и GH методом ДНК-диагностики. Установлено, что среди молочного скота преимущественно встречаются особи с генотипом PRL АА (70,0—77,5%), с частотой аллеля А гена пролактина — 0,83—0,87, аллеля В — 0,13—0,17. Встречаемость аллеля В была выше среди холмогорских коров (0,16—0,17). В стаде полновозрастных животных черно-пестрой породы имеется сдвиг генетического равновесия в сторону генотипов PRL AA и PRL BB (χ2=3,97; Р<0,05). В исследуемых стадах крупного рогатого скота выражено преимущество генотипа GH LL с частотой встречаемости 51,0—79,2%. Встречаемость генотипа GH VV у черно-пестрого скота составила 2,0—2,4%, у холмогорского — 5,6—7,0%. Аллель L гена соматотропина преобладает над аллелем V в обеих породах. При этом чаще встречаемость аллеля L GH среди коров холмогорской породы (0,28—0,29). У холмогорских коров больше представлены редкие и желательные аллели генов PRL и GH. Чтобы повысить встречаемость «предпочтительных» в хозяйственном отношении генотипов и аллелей ДНК-маркеров, необходимо в большей степени использовать быков-производителей с желательными аллелями PRL и GH, а именно В и V соответственно. The polymorphism was studied and the frequency of occurrence of allelic variants for the genes of prolactin (PRL) and somatotropin (GH) was determinedin cows in two bred livestock farms of the Republic of Tatarstan. The object of the study were animals of the black-and-white breed of the first (n=151) and third (n=168) calving and the Kholmogory breed of the first (n=160) and third (n=143) calving. Genotyping of cows for PRL and GH genes was carried out using method of DNA diagnostics. It was revealed that there were mainly individuals with the PRL AA genotype (70,0–77,5%)among dairy cattle, the frequency of the A allele of the prolactin gene was 0,83–0,87, the B allele was 0,13–0,17. The frequency of allele B was higher among Kholmogory cows (0,16–0,17). There was a shift in the genetic balance towards the PRL AA and PRL BB genotypes (χ2=3,97; Р<0,05) in the herd of full-age animals of the black-and-white breed. The advantage of the GH LL genotype was expressed with a frequency of occurrence 51,0–79,2% in the studied cattle herds. The occurence of the GH VV genotype was 2,0–2,4%in black-and-white cattle, 5,6–7,0% was in Kholmogorycattle. The L allele of the somatotropin gene predominated over the V allele in both breeds. Moreover, the occurrence of the L GH allele among cows of the Kholmogory breed (0,28–0,29) was more often. Rare and desirable alleles of the PRL and GH genes are more represented in Kholmogory cows. In order to increase the occurrence of economically “preferred” genotypes and alleles of DNA markers, it is necessary to use more stud bulls with the desired PRL and GH alleles, namely B and V, respectively.

2020 ◽  
Vol 17 ◽  
pp. 00060
Author(s):  
Radik Shaidullin ◽  
Gazimzyan Sharafutdinov ◽  
Anastasia Moskvicheva ◽  
Rustam Ravilov ◽  
Ismagil Khakimov

The article studies the duration of use and life-long productivity black-motley breed cows with different allelic variants of the kappa-casein and diacylglycerol O-acyltransferase genes. The research consists the data study on cows dropped out the herd during 2012–2016 in the Dusym LLC of Atninsky District in the Republic of Tatarstan. The cows were previously genotyped for CSN3 and DGAT1. The herd of black-and-white cattle consisted the genotypes CSN3 AA and DGAT1 AK with a frequency of 60.9 and 53.5 % and less often CSN3 BB and DGAT1 KK – 4.0 and 6.3 %. Moreover, the frequency of occurrence of the desired B allele of the CSN3 gene was 0.22, and the K allele of DGAT1 was 0.33. Cows with the CSN3 AB genotype reliably surpass animals with the CSN3 AA genotype in the duration of use by 0.39 lactations (P <0.05), life-long milk yield by 2401 kg of milk (P <0.05), and the amount of milk fat by 90 kg (P <0.05), mass fraction of protein in milk – by 0.04 % (P <0.01), the amount of milk protein – by 83 kg (P <0.05). Animals with the DGAT1 KK genotype also have an excess over other groups of cows in terms of productive longevity, but the difference is significant only in fat milk (P <0.01–0.001).


2020 ◽  
Vol 5 (2) ◽  
pp. 59-63
Author(s):  
Radik Shaydullin ◽  
Gazimzyan Sharafutdinov ◽  
Anastasiy Moskvicheva

The purpose of the research is improving the quality and technological properties of milk from black-and-white cows. The research was conducted on first-calf cows of a black-and-white breed in LLC «Dusym» of the Atninsky district of the Republic of Tatarstan. Three groups of experimental cows were formed depending on the genotype of CSN3 and DGAT1, which have equal lactation month. Cheese making milk obtained from cows with different allelic variants of the kappa-casein and diacylglycerol O-acyltransferase genes was studied. DNA testing was performed on the CSN3 and DGAT1 genes of experimental cows and allelic variants of these genes were determined by pol-ymerase chain reaction followed by analysis of restriction-site polymorphism. It was found that milk of cows with the genotype CSN3 AB and CSN3 BB, when exposed to rennet, showed a better yield of a dense casein clot (80 and 100%) and separation of serum (P <0.05), with a shorter coagulation time (20.3 and 16.5 min; P <0.05-0.01). Ac-cording to the DGAT1 gene, first-calf milk with the allelic variant DGAT1 AK and DGAT1 KK had the advantage, respectively, 86.7% and 85.7%, 24.0 and 25.7 minutes. The worst milk coagulation time is typical for CSN3 AA group – 29.5 minutes and DGAT1 AA one – 30.4 min. In terms of milk coagulation time, type II (15-40 min.) groups have the genotype CSN3 AB and CSN3 BB with the largest share of cows in the herd – 80.0 and 71.4% and with the genotype DGAT1 AK and DGAT1 KK – 86.6 and 85.7%, respectively. Thus, milk obtained from cows with the kappa-casein gene allele B and the diacylglycerol O-acyltransferase gene allele K gene in the genotype has the best cheese-making properties.


REPORTS ◽  
2020 ◽  
Vol 6 (334) ◽  
pp. 109-114
Author(s):  
K.Zh. Zhumanov ◽  
◽  
A.D. Baimukanov ◽  

The article deals with the productive parameters of cows of dairy cattle of the Holstein black-and-white breed in Kazakhstan. The research aimed to determine the level of productivity of the first-calf heifers of the Holstein black-and-white cattle. As an object of the research, information was used on first-calf heifers lactating in 2016-2017 in the breeding herds of the Holstein black-and-white cattle of the Republic of Kazakhstan. The source was the official data of the information-analytical system (IAS) of the livestock breeding of the Republic. The data analysis on the dairy productivity of the cows-daughters of the estimated servicing bulls is carried out according to the indicators of milk yield, the fat and protein content in milk, the yield of milk fat and protein for 305 days of lactation and the research period.


Author(s):  
Шайдуллин ◽  
Radik Shaydullin ◽  
Шарафутдинов ◽  
Gazimzyan Sharafutdinov ◽  
Сибагатуллин ◽  
...  

Thanks to DNA diagnostics method it has become possible to identify the genotypes of marker genes of servicing bulls, that makes effective use of manufacturers with the desired gene alleles of economically useful features and is now the main task of breeding in cattle. The analysis and synthesis of polymorphism according to DNA markers of productivity: the kappa-casein (CSN3), diacylglycerol O-acyltransferase (DGAT1), beta-lactoglobulin (LGB), prolactin (PRL), growth hormone (GH), thyroglobulin (TG5), leptin ( LEP) in bulls of Tatarstan type of Kholmogorsk breeding, black-and-white and Kholstein breeding, belonging to tribal enterprises of Tatarstan: JSC Parent enterprise “Elita”, JSC “Menzelinsky breeding enterprise”, JSC “Bugulminsk breeding enterprise”. It was found that the bulls of Tatarstan type of Kholmogorsk breeds have the highest frequency of heterozygous genotypes for most genes, so according to CSN3 genotype AB - 34.2%, according to PRL genotype AB - 30.4%, to TG5 genotype TC - 35.9%, to LEP genotype TC - 66.7% and homozygous genotypes for the genes DGAT1, LGB, PRL, GH, LEP, respectively, AA genotype (47.2%), BB (48.7%), BB (2.2%), VV (20,0%), SS (33.3%). Genetic equilibrium is disrupted on gene of diacylglycerol acyltransferase in bulls of Tatarstan type of Kholmogory breed (χ2 = 4,72; P <0.05) and a black-and-white breed (χ2 = 3,91; P <0.05), and according to the somatropin gene only Kholmogory breed producers (χ2 = 5,42; P <0.05).


2020 ◽  
Vol 17 ◽  
pp. 00059 ◽  
Author(s):  
Radik Shaidullin ◽  
Gazimzyan Sharafutdinov ◽  
Anastasia Moskvicheva ◽  
Tagir Faizov ◽  
Yusupzhan Yuldashbaev

The inheritance of allelic variants of the kappa-casein gene from bulls with different genotypes and their influence on the milk production of daughters were studied. For research, PCR diagnostics of daughters in the herds of black-and-white cattle at the farms “Azeleevo” and “Vakhitovo” (OJSC “Red East Agro”) was carried out, the fathers were two bulls LADDIE 135797213 with a genotypes CSN3 AB and LOMAX 10785322 with a genotype CSN3 BB. Based on the results of genotyping, the frequency of occurrence of genotypes AA, AB, BB kappa-casein and allelic variants A and B in daughters was calculated. The bull with the CSN3 AB genotype produced the largest number of offspring with the CSN3 AA genotype 50.0-51.8 %, and the bull with the homozygous BB genotype produced 24–25 % of daughters carrying the BB variant and 75 -76 % of the AB genotype. The frequency of occurrence of the Kappa-casein allele in the daughters of bulls with the genotype CSN3 BB was 0.63, which is almost 2 times more than the allele A. When comparing heifers with the same genotype (CSN3 AB), it was found that at the Azeleevo farm, the daughters of the bulls having the genotype BB of kappa-casein are superior to cows born from bulls with the genotype AB by the milk yield (+36 kg), mass fraction of fat and protein (+0.01 %), the amount of milk fat (+2 kg) and protein (+2 kg). In the group of first-calf heifers with the BB genotype, daughters of the producer with the BB genotype are superior by the milk yield (+ 175 kg), the mass fraction of fat in milk (+0.1 %), the amount of milk fat (+12 kg), the mass fraction of protein in milk (+0.09 %), the amount of milk protein (+11 kg). At the Vakhitovo farm, similar results in the group of CSN3 AB heifers born from the bulls with the BB genotype was observed


Author(s):  
N. V. Kazarovets ◽  
I. P. Sheyko ◽  
T. V. Pavlova

The leading element in breeding programs for breeding and improving the breeds of dairy cattle in highly developed countries is reproduction of high-quality producing bulls. The paper presents the results of producing bulls breeding when creating Belarusian black-and-white breed of dairy cattle, techniques and methods of obtaining improving bulls when improving the Holsteinized dairy cattle of the republic at the present stage. It is shown that historically evolution of the breeding process of reproduction of domestic black-and-white bulls is based on imported breeding material from different countries (bulls, semen, heifers). Analysis of solution to the problem of using imported animals is presented. Periods of creating a breeding base for reproduction of imported material have been determined, methodological approaches and possibilities of breeding domestic producing bulls in the republic have been specified. Efficiency of breeding business for introduction of modern methods and techniques for assessing and reproduction of high quality breeding animals has been revealed. The periods of breeding process for reproduction of domestic producing bulls have been chronologically distinguished, including three stages: the first 1930-1990, the second 1990-2000, the third 2000 to the present. Substantiation of these time stages is based on the results of breeding activities carried out by scientists and breeders of the republic, and the social and economic conditions of those periods. Analysis of the created breeding base for reproduction of imported material has been carried out, including both breeding stock of breeding plants for parental forms breeding, as well as state breeding enterprises for rearing, evaluating and using the producing bulls. Normative legal documents developed by specialists of state services, scientists of the Belarusian Research Institute for Animal Breeding for the scheduled formation of the breeding base of dairy cattle in the republic have been indicated. The research results will make it possible to develop a concept for reproduction of highly valuable domestic improving bulls in terms of breeding for formation of Belarusian Holstein dairy cattle meeting modern production technology requirements in the context of intensification of the industry based on increase in the role of genetic factors. 


Author(s):  
A.S. Davydova ◽  
E.G. Fedosenko

Рост продуктивности молочного скота и качественное улучшение существующих пород на основе совершенствования методов и форм племенной работы является одним из эффективных способов увеличения объёмов производства молока. В Костромской области ведётся работа по разведению четырёх пород молочного и молочно-мясного направлений продуктивности: костромской, чёрно-пёстрой, ярославской и айрширской. По данным бонитировки за 2018 год, 44,4 скота относится к костромской породе крупного рогатого скота. Удельный вес чёрно-пёстрого скота в структуре поголовья Костромской области достиг 47,4, на долю ярославской породы приходится 7,4 общего поголовья, а айрширской породы всего 0,8. Исследования показали, что самый высокий удой имели коровы чёрно-пёстрой породы (6773 кг), однако они уступали животным костромской породы по массовой доле жира в молоке и продолжительности хозяйственного использования. Повышение генетического потенциала коров и срока их продуктивного использования является одним из резервов интенсификации отрасли молочного скотоводства в Костромской области.The increase in the productivity of dairy cattle and the qualitative improvement of existing breeds by improving methods and forms of stock breeding is one of the effective ways of increasing milk production. In the Kostroma region work on the breeding of four breeds of dairy and dairy-beef productivity is in progress: Kostroma, Black-and-White, Yaroslavl and Ayrshire. According to 2018 livestock judgement 44.4 of cattle belong to the Kostroma breed of cattle. The share of black and white cattle in the structure of the livestock of the Kostroma region reached 47.4, the Yaroslavl breed accounts for 7.4 of the total livestock and the Ayrshire breed is only 0.8. Researches have shown that cows of Black-and-White breed (6773 kg) had the highest milk yield however they were inferior to Kostroma breed animals in the mass fraction of fat in milk and the duration of economic use. Increasing the genetic potential of cows and the term of their productive use is one of the reserves for intensifying the dairy farming in the Kostroma region.


Author(s):  
L. Ovchinnikova ◽  
E. Babich

In the Republic of Kazakhstan over the past decade positive changes have been observed in increasing the number of farm animals, increasing their productivity, and increasing the gross production of milk, meat and other products. At the same time the development of dairy cattle breeding is mainly due to the breeding of Black-and-White, Red Steppe, Simmental breeds and their intra-breed types as well as imported from abroad Holstein breed. The purpose of the research was to analyze ways to improve the intra-breed type Karatomar of Black-and-White breed in Northern Kazakhstan. Research work has been carried out on breeding animals such as Karatomar type cattle of Black-and-White breed in LLP “Experimental farm Zarechnoe” in the Kostanay district in the Kostanay region. The dynamics of growth and development indicators, reproductive traits of heifers of a new intra-breed type Karatomar of Black-and-White cattle have been studied. A comprehensive assessment of their economically useful traits in the first lactation, determined by the resistance of animals to climatic and forage conditions, the efficiency of breeding of animals of different genotypes has been conducted. It has been found that the replacement heifers – daughters of American breeding sires inherited precocity and showed higher growth energy after 6 months of age and continued to grow rapidly until 18 months. The advantage in metabolism was in the daughters of Holstein sires of the American selection Orbit 4078, and the increased immune status of the organism in the daughters of the sire of the domestic selection Yamal 975 as the most adapted to the conditions of Northern Kazakhstan. The best ability to increasing the milk yield, stable lactation activity, high milk productivity, gross content of dry matter and nonfat milk solids have been characterized by the daughters of sires of the American selection Orbit 4078 and Lowrider 4129. Analysis of economic indicators of milk production has been shown that from cows – daughters of sires Orbit 4078 and Lowrider 4129 for 305 days of lactation received 15,8 and 15,2 % more milk of base fat, the expenditures of EFU per 1 kg of production was lower by 10,2 and 5,9 %. A higher level of profitability has been observed in the group of daughters of the sires Orbit 4078 – 35,5 % and Lowrider 4129 – 33,1 %, which was 8,5 and 10,8 % higher than in the group of daughters of the sire Yamal 975.


2020 ◽  
Vol 11 (2) ◽  
pp. 1511-1514
Author(s):  
Sergey Yu. Smolentsev ◽  
Galiya R Yusupova ◽  
Nikita V Nikolaev ◽  
Zaira F Aukhadieva ◽  
Renat A Volkov ◽  
...  

The effectiveness of dairy cattle breeding largely depends on the intensity of use of the breeding stock. At the same time, the factor of the duration of economic use of animals is gaining importance, which affects not only the production economy, but also the improvement of stocks and breeds. The lifelong productivity, the quantitative and qualitative growth of the stock, the amount of capital investment in its formation and the efficiency of operation depend on the term for the productive use of cows. The terms of breeding and production use of animals are direct linked with the biologically possible longevity of each animal, in turn, the duration of the productive period of cattle is in the range of 12-17 lactations. The influence of feed on production is associated primarily with the level of digestion and assimilation. The digestive capacity of the digestive tract of animals can be improved by selecting the optimal ratio in the diet of rough, juicy and concentrated feeds, i.e. optimization of animal feeding types. We conducted an analysis of milk productivity in lactating cows, when using AVMC feed additive in the diet. The experiment was conducted in the conditions of the dairy farm of Biryuli CJSC of the Vysokogorsky District of the Republic of Tatarstan on three groups of lactating cows. For the experiment, 30 cows were selected, 10 in each Holstein black-and-white breed according to the principle of analogues. Studies have shown that AVMC with rapeseed oil seeds and AVMC with fus in the diet of lactating cows provided higher animal productivity and positively affected the chemical composition of milk.


2021 ◽  
pp. 19-23
Author(s):  
Т.В. Павлова ◽  
Н.Н. Шамич ◽  
В.А. Бельцов

Цель работы – установить влияние генотипа и интенсивности формирования коров на уровень их молочной продуктивности в одном из предприятий Минской области Республики Беларусь. Объектом исследований являлись 940 коров белорусской чёрно-пёстрой породы с разной долей генотипа по голштинской породе. Для проведения группировки коров по генотипам у каждого животного определяли линейную принадлежность, породность по голштинской породе и страну селекции отца. При оценке молочной продуктивности коров учитывались следующие показатели: удой за 305 дней первой и последней законченной лактации; массовая доля жира, %; массовая доля белка, %; выход молочного жира и белка, кг. Установлено, что с увеличением породности по голштинской породе у коров в стаде рассматриваемого предприятия прослеживается повышение удоев и снижение жирно- и белковомолочности. Наиболее высокий удой наблюдается у коров шведской (9190 кг) и итальянской (8148 кг) селекций, удой этих коров достоверно (Р = 0,999) превышает средний по стаду на 2019 и 977 кг соответственно; массовая доля жира и белка в молоке – у коров российской селекции (4,26 и 3,54% соответственно); у животных белорусской селекции удой составил 6714 кг при жирно- и белковомолочности 4,24 и 3,49% соответственно. С увеличением интенсивности формирования животных снижается возраст первого осеменения и увеличивается живая масса при первом осеменении, наиболее близки к оптимальным параметры у умеренноформирующихся животных. У них же отмечен и наиболее высокий удой за 305 дней первой лактации – 7226 кг, что достоверно (Р = 0,95) превышает этот показатель у медленно- и быстроформирующихся животных на 264 и 150 кг соответственно. The purpose of the work is to establish the influence of the genotype and intensity of cows’ formation on the level of their lactation performance in one of the enterprises of the Minsk region of the Republic of Belarus. The object of research was 940 cows of the Belarusian Black-and-White breed with different genotype proportions for the Holstein breed. To group cows by genotypes, each animal was determined by directional affiliation, race by Holstein breed and country of father selection. When assessing the lactation performance of cows, the following indicators were taken into account: yield for 305 days of the first and last completed lactation; weight fraction of fat, %; weight fraction of protein, %; milk fat and protein yield, kg. It was established that with an increase in breeding in the Holstein breed, cows in the herd of the enterprise under consideration show an increase in yields and a decrease in fat and protein milking capacity. The highest yield is observed in cows of Swedish (9190 kg) and Italian (8148 kg) selection, the yield of these cows significantly (P = 0.999) exceeds the herd average yield for 2019 and 977 kg, respectively; mass fraction of fat and protein in milk – in cows of Russian selection (4.26 and 3.54%, respectively); in animals of Belarusian selection the yield was 6714 kg with fat and protein milking capacity of 4.24 and 3.49%, respectively. As the intensity of animal formation increases, the age of the first insemination decreases and the live weight increases at the first insemination, the parameters are closest to optimal in moderately forming animals. They also had the highest yield for 305 days of the first lactation – 7226 kg, which significantly (P = 0.95) exceeds this indicator in slow and fast-forming animals by 264 and 150 kg, respectively.


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