Evaluation of Cellulose Nanofi bers by Using Sedimentation Method

The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P<0.05). The percent of agreement between methods was 41%. Over a total of 27 positive samples detected by the FSSM, the FSM missed 16, yielding 60% false negative samples. The FSSM enhanced 2.5 times the sensitivity of diagnosis. The complexity of the FSM may decrease its sensitivity through missing and loss of eggs during sample processing. These results confirmed that the commonly used FSM underestimates the prevalence and the egg output in cattle and that the FSSM is a more reliable diagnostic method.

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Coagulation-sedimentation Method Using Aluminum Sulfate and Calcium Hydroxide for Removal of Boric Acid from Wastewater. Improvement of Removal Rate of Boric Acid by Addition of Gypsum into Wastewater.

NIPPON KAGAKU KAISHI ◽

10.1246/nikkashi.2002.265 ◽

2002 ◽

pp. 265-268 ◽

Cited By ~ 5

Author(s):

Satoshi KUDO ◽

Masahiro SAKATA

Keyword(s):

Boric Acid ◽

Calcium Hydroxide ◽

Aluminum Sulfate ◽

Removal Rate ◽

Sedimentation Method

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Usage of Speed Sedimentation Method for Enzyme Degradation of Chitosan

Chemical and Biochemical Technology ◽

10.1201/b17717-13 ◽

2014 ◽

pp. 131-138 ◽

Cited By ~ 1

Keyword(s):

Sedimentation Method ◽

Enzyme Degradation

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Sedimentation Method to Evaluate PFI Mill Beating Degree of Wood Pulp Fibers

Sen i Gakkaishi ◽

10.2115/fiber.60.112 ◽

2004 ◽

Vol 60 (4) ◽

pp. 112-117 ◽

Cited By ~ 1

Author(s):

Bo Chen ◽

Daisuke Tatsumi ◽

Takayoshi Matsumoto

Keyword(s):

Wood Pulp ◽

Pulp Fibers ◽

Sedimentation Method

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Particle Size Determination by Radioisotope X-Ray Absorptiometry Using Sedimentation Method

RADIOISOTOPES ◽

10.3769/radioisotopes.25.9_534 ◽

1976 ◽

Vol 25 (9) ◽

pp. 534-539 ◽

Cited By ~ 1

Author(s):

Yoshiko MATSUI ◽

Tomihiko FURUTA ◽

Soji MIYAGAWA

Keyword(s):

Particle Size ◽

Size Determination ◽

X Ray ◽

Sedimentation Method ◽

Particle Size Determination

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Motilitas dan viabilitas spermatozoa semen sexing kambing peranakan etawa (pe) menggunakan metode sedimentasi putih telur dengan pengencer yang berbeda

Jurnal Sains Peternakan ◽

10.21067/jsp.v5i1.3140 ◽

2017 ◽

Vol 5 (1) ◽

pp. 50-56 ◽

Cited By ~ 1

Author(s):

Yanpiterson Umbu Lele ◽

Enike Dwi Kusumawati ◽

Aju Tjatur Nugroho Krisnaningsih

Keyword(s):

Significant Influence ◽

Research Method ◽

Egg Yolk ◽

Egg White ◽

Variance Analysis ◽

Laboratory Research ◽

Sedimentation Method ◽

Randomized Design ◽

Completely Randomized Design

ABSTRAK Tujuan penelitian ini adalah untuk mengetahui motilitas dan viabilitas spermatozoa semen hasil sexing kambing Peranakan Etawa (PE) dengan metode sedimentasi putih telur menggunakan pengencer yang berbeda. Materi yang digunakan dalam penelitian ini adalah semen segar kambing Peranakan Etawa (PE) berumur 2 tahun dan bobot badan 120 kg dari Balai Besar Inseminasi Buatan (BBIB) Singosari Malang. Metode penelitian ini yang digunakan adalah penelitian laboratorium dengan menggunakan rancangan acak lengkap (RAL). Perlakuan terdiri dari semen sexing dengan menggunakan pengencer Andromed lapisan atas dan Andromed lapisan bawah dan Tris Aminomethan Kuning Telur lapisan atas dan Tris Aminomethan Kuning Telur lapisan bawah dengan masing-masing 10 ulangan. Variabel yang diamati adalah motilitas dan viabilitas spermatozoa semen sexing dengan pengencer yang berbeda, proses sexing menggunakan metode sedimentasi putih telur. Data yang diperoleh dianalisis menggunakan Analisis Varian (ANOVA) apabila perlakuan memberikan perbedaan, maka dilanjutkan dengan Uji BNT. Hasil penelitian menunjukkan bahwa metode sedimentasi putih telur dengan pengencer yang berbeda pada kambing Peranakan Etawa (PE) memberikan pengaruh yang sangat nyata (P<0,01) terhadap motilitas dan viabilitas spermatozoa semen sexing. Motilitas dan viabilitas terbaik terdapat pada pengencer Tris aminomethan kuning telur lapisan atas sebesar 75,65%, 74,41% dan Andromed lapisan atas sebesar 65,4%, 60%. Berdasarkan hasil penelitian dapat disimpulkan bahwa kualitas spermatozoa semen sexing kambing PE dengan menggunakan pengencer Tris aminomethan memberikan hasil terbaik terhadap motilitas dan viabilitas. Berdasarkan hasil penelitian ini maka disarankan agar menggunakan pengencer Tris aminomethan kuning telur sebagai pengencer sexing spermatozoa. ABSTRACT The purpose of this study was to determine the motility and viability of PE sperm sexing with egg white sedimentation method using different diluents. This research method used was laboratory research using a completely randomized design (CRD) treatment PE sexing sperm. The results showed that the quality of PE sperm goat with various diluents showed a significant influence (P<0,01). The variables observed were motility and viability of sperm. The data obtained were analyzed using variance analysis (ANOVA) with complete randomized design (CRD). Motility and viability at best against Tris aminomethane top layer of egg yolk 65,4% and the top layer of Andromed 75,65%, 74,41%. Based on the results it is suggested that using Tris aminomethane yolk as a diluents sexing sperm. It can be concluded that the quality of sexing sperm goat PE by using tris aminomethane diluent gives the best result on motility and viability.

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Contamination of Soil Transmitted Helminths (STH) Eggs in Raw Vegetables at Street Food Stalls and Restaurant in Lorok Pakjo Village, Palembang

BioScientia Medicina ◽

10.32539/bsm.v5i4.397 ◽

2021 ◽

Vol 5 (4) ◽

pp. 1133-1141

Author(s):

Leo Medianto Faziqin ◽

Dalilah ◽

Dwi Handayani ◽

Chairil Anwar ◽

Susilawati

Keyword(s):

Observational Study ◽

Ascaris Lumbricoides ◽

Tropical Climate ◽

The Other ◽

Street Vendors ◽

Street Food ◽

Soil Transmitted Helminths ◽

Sedimentation Method ◽

Fresh Vegetables ◽

The World

Background: Infection Soil Transmitted Helminths (STH) is a disease that is a problem in the world, especially in areas with a tropical climate like Indonesia. This infection can be transmitted through consumption of raw vegetables contaminated with STH. This study aims to determine whether there is contamination of STH eggs in raw vegetables at street food stalls and restaurants in Lorok Pakjo Village, Palembang. Methods: This research is a descriptive observational study. Samples in the form of fresh vegetables were collected from 18 street stalls and 14 restaurants in Lorok Pakjo Village, Palembang. Samples were examined using the sedimentation method and observed using a microscope. Results: The results obtained 80 samples consisting of 30 cabbage, 18 basil, 30 cucumber, 1 lettuce, and 1 eggplant. Of the 80 samples, there were 20 (25%) positive samples contaminated with STH eggs consisting of 8 (10%) cabbage samples, 8 (10%) basil samples, 4 (5%) cucumber samples, and no STH contamination was found in lettuce. and eggplant. Of the 20 positive samples of STH contamination, 12 samples came from street vendors consisting of 5 (10.6%) cabbage samples, 6 (12.8%) basil samples, and 1 (2.1%) cucumber samples. While the other 8 samples came from restaurants consisting of 3 (9.1%) cabbage samples, 2 (6%) basil samples, and 3 (9.1%) cucumber samples. Found only eggs of Ascaris lumbricoides infertile decorticate in all samples consisting of 9 eggs on cabbage, 16 eggs on basil, and five eggs on cucumber. Conclusion: There is contamination of eggs Soil Transmitted Helminths in raw vegetables in street food stalls and restaurants in Lorok Pakjo Village, Palembang.

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