scholarly journals Studies on Analysis of Antioxidant and Enzyme Inhibitory Activity of Vitex negundo Linn.

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Ved Prakash ◽  
Shelly Rana ◽  
Anand Sagar
Keyword(s):  
Urease Activity ◽  
Therapeutic Potential ◽  
Radical Scavenging Activity ◽  
Scientific Evidence ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Vitex Negundo ◽  
Leaf Extracts ◽  
Treatment Of Obesity ◽  
Dpph Radical Scavenging

The current study was designed to investigate the leaf extracts of Vitex negundo Linn. for their antioxidant and enzyme inhibitory (α-amylae and urease) activity. The antioxidant capacity of the different extracts (methanol, acetone and aqueous) of this plant was evaluated by DPPH (1,1-diphenyl-2-picrylhydrazyl) and reducing power tests. The plant exhibited good DPPH radical scavenging activity and moderate reducing power potential Further, all the extracts of V. negundo were reported to possess good anti-alpha amylase and anti-urease activity of greater than 50% in all the solvents used at a concentration of 1 mg/mL. Thus the study provided scientific evidence to the traditional uses of this plant in the treatment of obesity, diabetes, ulcers, kidney stones etc. Therefore, the leaf extracts of this plant can be selected for further investigation to determine their therapeutic potential.

scholarly journals Antiglycation and Antioxidant Properties of Ficus deltoidea Varieties

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Nur Sumirah Mohd Dom ◽  
Nurshieren Yahaya ◽  
Zainah Adam ◽  
Nik Mohd Afizan Nik Abd. Rahman ◽  
Muhajir Hamid
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protein Carbonyl ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Ficus Deltoidea ◽  
Dpph Radical Scavenging

The present study aimed to evaluate the potential of standardized methanolic extracts from seven Ficus deltoidea varieties in inhibiting the formation of AGEs, protein oxidation, and their antioxidant effects. The antiglycation activity was analyzed based on the inhibition of AGEs, fructosamine, and thiol groups level followed by the inhibition of protein carbonyl formation. The antioxidant activity (DPPH radical scavenging activity and reducing power assay) and total phenolic contents were evaluated. After 28 days of induction, all varieties of Ficus deltoidea extracts significantly restrained the formation of fluorescence AGEs by 4.55–5.14 fold. The extracts also reduced the fructosamine levels by 47.0–86.5%, increased the thiol group levels by 64.3–83.7%, and inhibited the formation of protein carbonyl by 1.36–1.76 fold. DPPH radical scavenging activity showed an IC50 value of 66.81–288.04 μg/ml and reducing power activity depicted at 0.02–0.24 μg/ml. The extent of phenolic compounds present in the extracts ranged from 70.90 to 299.78 mg·GAE/g. Apart from that, correlation studies between the activities were observed. This study revealed that seven varieties of Ficus deltoidea have the potential to inhibit AGEs formation and possess antioxidant activity that might be attributed to the presence of phenolic compounds.

scholarly journals Antioxidants from the Leaves of Cinnamomum kotoense

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Kuo-Chen Cheng ◽  
Man-Chun Hsueh ◽  
Hou-Chien Chang ◽  
Alan Yueh-Luen Lee ◽  
Hui-Min Wang ◽  
...  
Keyword(s):  
Potential Role ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Novel Antioxidants ◽  
Dpph Radical Scavenging

Two novel antioxidants, obtusilactone A (1) and (-)-sesamin (2) have been identified in Cinnamomum kotoense Kanehira. Both showed effective 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity compared with vitamin C, and reducing power compared with BHA. These results suggest that these constituents of C. kotoense act as natural antioxidants and play a potential role in cancer prevention.

In Vitro Free Radical Scavenging Activity and Total Flavonoid of Abelmoschus manihot (L.) Medic Extracts

2011 ◽  
Vol 140 ◽  
pp. 355-359 ◽  
Author(s):  
Yan Qiu ◽  
Jian Jun Song
Keyword(s):  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Flavonoid ◽  
Scavenging Activity ◽  
Ic50 Values ◽  
Hydrogen Radicals ◽  
Dpph Radical Scavenging

The Ablmoschus manihot (L.) Medic, an edible hibiscus of the Malvaceae family, is abundant with total flavonoid. The content of total flavonoid from A. manihot flowers (TFA) was extracted by 70% ethanol (ethanol:water, 70:30). The scavenging effects of TFA on superoxide anions and hydrogen radicals were related to the total flavonoid concentrations with the IC50 values of 63.90 ±2.21 μg/mL and 266.88±28.32 μg/mL, respectively. However, the IC50 values of standard ascorbic acid were 436.52 ±14.36 μg/mL and 439.58±21.41 μg/mL, respectively. The DPPH radical scavenging activity increased with increasing of TFA concentrations and the highest inhibition was 94.63 ±3.01% at 50 μg/mL, as compared to ascorbic acid 34.94 ±1.50%. The extract also showed good reducing power. The data from this study suggest A. manihot flower extract has significant potential to use as health supplements and nutraceuticals.

Chemical Composition of Essential Oil and Antioxidant Activities of Curcuma petiolata Roxb. Rhizomes

2012 ◽  
Vol 506 ◽  
pp. 393-396 ◽  
Author(s):  
A. Thakam ◽  
N. Saewan
Keyword(s):  
Ascorbic Acid ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Etoac Extract ◽  
Dpph Radical Scavenging

Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract > DCM extract > n-butanol extract > aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P<0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction

scholarly journals In-vitro Antioxidant Activity and Preliminary Phytochemical Analysis of Different Leaf Extracts of Hemionitis arifolia

2021 ◽  
pp. 281-291
Author(s):  
Rajendran Raja Priya ◽  
N. Bhadusha ◽  
Veramuthu Manivannan ◽  
Thanthoni Gunasekaran
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Hydroalcoholic Extract ◽  
Reducing Power Assay

Objective: To evaluate the preliminary phytochemical content and antioxidant potential of the hydroalcoholic leaf extracts of Hemionitis arifolia. Methods: Total phenolic, flavonoid and alkaloid contents were evaluated using spectrophotometric methods. The free radical scavenging activity of the leaf hydroalcoholic extract were evaluated against DPPH+, ABTS+, Reducing power assay and nitric oxide assay were determined. Results: The hydroalcoholic concentrate of H. arifolia uncovered the most elevated polyphenol content when contrasted and the other phytoconstituents. Absolute phenol content of the hydroalcoholic separate was observed to be 31.78%, flavonoid content is 1.02% and Alkaloid content is 30.40% individually. The Solvent concentrates showed huge cell reinforcement movement, with hydroalcoholic extract. ABTS Assay, DPPH assay, Reducing power assay and Nitric oxide assay where the Inhibition concentration were 667.75µg/ml, 734.25 µg/ml, 791.58 µg/ml and 899.67 µg/ml. Conclusion: This study suggests that hydroalcoholic leaf extracts of H. arifolia could be a potential source of natural antioxidant and justifies its use in ethno-medicine.

scholarly journals Comparison of Depolymerization of Chitosan into Chitooligosaccharides from the Shrimp Shell Waste of Parapeneopsis Stylifera by Non-Specific Enzymes

2021 ◽  
Author(s):  
V. Renuka ◽  
Chandragiri Nagaraja Rao Ravishankar ◽  
Elavarasan Krishnamoorthy ◽  
Abubacker Aliyamveetil Zynudheen
Keyword(s):  
Thermal Behavior ◽  
Antioxidant Activities ◽  
Proteolytic Enzymes ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Structural Difference ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

Abstract Two different non-specific proteolytic enzymes (papain and pepsin) and two carbohydrase enzymes (α-amylase and β-amylase) were used for the depolymerization of chitosan to produce chitooligosaccharides (COS). The COS produced using papain, pepsin, α-amylase and β-amylase were characterized for physico-chemical, structural, thermal and antioxidant activities. Chitooligosaccharides produced using pepsin enzyme had higher solubility compared to other three COS. Structural variation of chitosan and their oligosaccharides were studied by FTIR analysis and it revealed the existence of various structural difference among the oligosaccharides and chitosan. Thermal behavior of chitosan and oligosaccharides were examined using DSC analysis, and it exhibits difference in the glass transition temperature among the four oligosaccharides and native chitosan. Anti-oxidant analysis such as DPPH radical scavenging activity and reducing power revealed the antioxidant ability of chitooligosaccharides. Among the four COS, pepsin-COS had the highest DPPH radical scavenging and reducing power activity. According to the patterns of molecular weight reduction, antioxidant properties, thermal behavior properties, four enzymes α- amylase, β-amylase, pepsin and papain found suitable for hydrolyzing chitosan into chitooligosaccharide. As it is superior to chitosan, chitooligosaccharide can be utilized in food industry as bio-preservatives to enhance the quality, safety and shelf life of the products.

scholarly journals Callus formation, phenolics content and related antioxidant activities in tissue culture of a medicinal plant colocynth (Citrullus colocynthis)

2021 ◽  
Vol 10 (2) ◽  
pp. 79-94
Author(s):  
Farouk K. El-Baz ◽  
Amal A. Mohamed ◽  
Sami I. Ali
Keyword(s):  
Total Phenolics ◽  
Antioxidant Activities ◽  
Callus Formation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Callus Cultures ◽  
Scavenging Activity ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Callus cultures from stems, leaves and roots of colocynth were initiated on MS media supplemented with various combinations of 2,4 dichlorophenoxyacetic acid (2,4-D) with kinetin (KIN) and benzyladenine (BA) with α-naphthaleneacetic acid (NAA). The highest percentage of callus formation frequency (98.9%) was obtained from stem explants grown on MS media supplemented with (1.0 mg/L) 2,4-D + (1.0 mg/L) KIN. The total phenolics and flavonoid content of the colocynth callus cultures were measured. The results showed that the MS medium supplemented with 6.0 mg/L 2,4-D + 2.0 mg/L KIN (MD3) gave the highest content of total phenolics (19.2 mg/100g d.w.) in leaf-derived calli. The highest content of flavonoids (47.3 mg/100g d.w.) was obtained in stem derived calli grown on the same medium (MD3). Antioxidant activities of extracts were determined using different assays, including DPPH radical scavenging activity, hydrogen peroxide (H2O2) scavenging activity and ferric reducing power. Leaf-derived calli cultured on MS medium + 2.0 mg/L 2,4-D + 1.0 mg/L KIN (MD1) showed the highest DPPH radical scavenging activity (85.3%). The highest percentage of H2O2 scavenging activity (61.4%) was detected in leaf explant-derived calli growing on MD1. The leaf-derived calli growing on (MD3) gave the highest ferric reducing power (22.3 μg/g d.w.), compared to the activities of stems, leaves and roots of in vitro grown seedlings (3.28, 12.9 and 2.85 μg/g d.w.), which were used as controls. On the basis of the current findings, we conclude that MS media supplemented with different combinations of 2,4-D and KIN yields higher phenolics, flavonoids contents and antioxidant activities than MS media supplemented with BA and NAA.

Evaluation of In-vitro cytotoxic and antioxidant activity of methanolic extracts of Ipomoea carnea and Alternanthera sessilis

2016 ◽  
Vol 5 (08) ◽  
pp. 4763 ◽  
Author(s):  
Anushi Jain ◽  
Soumen Roy ◽  
Ambika Joshi* ◽  
Nitesh Joshi
Keyword(s):  
Antioxidant Activity ◽  
Therapeutic Potential ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Assay Method ◽  
Leaf Extracts ◽  
Alternanthera Sessilis ◽  
Cytotoxicity Activity ◽  
Ipomoea Carnea

Ipomoea carnea and Alternanthera sessilis are two common weeds in India which have shown to possess several medicinal properties. The present study pertains to assess the antioxidant and cytotoxicity activity of the leaf extracts of these plants. The extraction of leaf content of the plants was carried out by soxhlet method using methanol. The in vitro cytotoxicity assay was performed in vitro on vero cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method. In vitro antioxidant activity was carried out by DPPH radical scavenging method. The cytotoxicity activity for I. Carnea i.e. IC50 (50% growth inhibition) value was found to be 1mg/ml and the IC50 of A.sessilis was found to be 6.5mg/ml. The antioxidant activity of I. Carnea i.e. IC50 value was found to be 1200ug/ml whereas that of A. sessilis was found to be 400ug/ml. The cytotoxic activity was higher for Ipomoea extracts as compared to Alternanthera extracts. However, the DPPH free radical scavenging activity was higher for Alternanthera sessilis extracts as compared to Ipomoea carnea extracts. Both showed high in vitro activity, which indicates the therapeutic potential of these plants.

scholarly journals PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF MILLINGTONIA HORTENSIS (L)

2017 ◽  
pp. 162-167
Author(s):  
Janaki A. ◽  
Kaleena P. K. ◽  
Elumalai D. ◽  
Hemalatha P. ◽  
Babu M. ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Leaf Extracts ◽  
Micro Organisms ◽  
Dpph Radical Scavenging

Objective: Millingtonia hortensis Linn (Bignoniaceae) is commonly known as cork tree and Akash neem. Aim of studies to determine the antioxidant activity and antibacterial activity.Methods: The antioxidant activity of different solvent extracts were measured by chemical analyses involving the assay of 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity and super oxide radical scavenging activity.Results: Phytochemicals (secondary metabolites) screening of methanol, chloroform, ethanol, petroleum ether, aqueous leaf extracts revealed the presence of carbohydrates, tannins, saponins, flavonoids, alkaloids, betacyanins, phenols and coumarins.Conclusion: The presence of these phytochemicals and antioxidant capacity support the use of this plant as an antibacterial agent against the group of micro organisms tested. 

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