scholarly journals Optical recording of membrane potential activity

1999 ◽  
Vol 20 (4) ◽  
pp. 391-403
Author(s):  
Kohtaro Kamino ◽  
Yoko Momose-Sato ◽  
Katsushige Sato
Keyword(s):  
Membrane Potential ◽  
Optical Recording ◽  
Potential Activity

Optical recording of neuronal activity in an invertebrate central nervous system: simultaneous monitoring of several neurons

1977 ◽  
Vol 40 (6) ◽  
pp. 1281-1291 ◽  
Author(s):  
B. M. Salzberg ◽  
A. Grinvald ◽  
L. B. Cohen ◽  
H. V. Davila ◽  
W. N. Ross
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Membrane Potential ◽  
Action Potential ◽  
Neuronal Activity ◽  
Optical Method ◽  
Optical Recording ◽  
Supraesophageal Ganglion ◽  
Potential Activity ◽  
Favorable Conditions

1. Using an optical method for measuring membrane potential, we have been able to monitor action-potential activity simultaneously in 14 neurons of the supraesophageal ganglion of the barnacle. 2. Under favorable conditions, 4-mV synaptic potentials could also be detected optically.

Fluorescent potentiometric dyes for membrane-potential imaging

1994 ◽  
Vol 52 ◽  
pp. 166-167
Author(s):  
Leslie M. Loew
Keyword(s):  
Membrane Potential ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Optical Recording ◽  
Biological Processes ◽  
Major Focus ◽  
The Past ◽  
Excitable Systems ◽  
Major Application ◽  
The Impact

A major application of potentiometric dyes has been the multisite optical recording of electrical activity in excitable systems. After being championed by L.B. Cohen and his colleagues for the past 20 years, the impact of this technology is rapidly being felt and is spreading to an increasing number of neuroscience laboratories. A second class of experiments involves using dyes to image membrane potential distributions in single cells by digital imaging microscopy - a major focus of this lab. These studies usually do not require the temporal resolution of multisite optical recording, being primarily focussed on slow cell biological processes, and therefore can achieve much higher spatial resolution. We have developed 2 methods for quantitative imaging of membrane potential. One method uses dual wavelength imaging of membrane-staining dyes and the other uses quantitative 3D imaging of a fluorescent lipophilic cation; the dyes used in each case were synthesized for this purpose in this laboratory.

Sub-Millisecond Time Resolved Imaging of Voltage Changes in Excitable Cells and Tissues Using Multiple Site Optical Recording of Transmembrane Voltage (Msortv) and Molecular Probes

1997 ◽  
Vol 3 (S2) ◽  
pp. 803-804
Author(s):  
B.M. Salzberg ◽  
A.L. Obaid
Keyword(s):  
Membrane Potential ◽  
Conformational Changes ◽  
Molecular Probes ◽  
Optical Recording ◽  
Membrane Voltage ◽  
Excitable Cells ◽  
Time Resolved ◽  
Molecular Weights ◽  
Transmembrane Voltage ◽  
Time Resolved Imaging

Molecular indicators of membrane potential may be used to obtain sub-millisecond time resolved images of transient changes in membrane voltage in a variety of biological systems. These probes are small amphipathic molecules having molecular weights of 400-500, and dimensions on the order of 10 Angstroms, which bind to, but do not cross cell membranes, and change either their absorbance or fluorescence in response to membrane voltage. These extrinsic optical signals depend linearly upon membrane potential, and the best of the dyes respond to a step change in voltage in less than 1.5 μsec at room temperature. The salient properties of fast potentiometric probes will be discussed, and the fidelity of optical recordings to transmembrane voltage changes will be considered.Since voltage changes in excitable cells take place on a time scale that is determined by the kinetics of conformational changes in membrane proteins, and by membrane electrical time constants, these changes tend to be very rapid, and resolving them requires imaging systems that are frequently orders of magnitude faster than usual video rates.

Optical recording of membrane potential in dissociated mouse vestibular ganglion cells using a voltage-sensitive dye

2000 ◽  
Vol 27 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Shi-Ming Yang ◽  
Tadashi Doi ◽  
Mikiya Asako ◽  
Ayumi Matsumoto ◽  
Toshio Yamashita
Keyword(s):  
Membrane Potential ◽  
Ganglion Cells ◽  
Optical Recording ◽  
Voltage Sensitive Dye ◽  
Vestibular Ganglion ◽  
Vestibular Ganglion Cells

scholarly journals Pauses in cholinergic interneuron firing exert an inhibitory control on striatal output in vivo

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Stefano Zucca ◽  
Aya Zucca ◽  
Takashi Nakano ◽  
Sho Aoki ◽  
Jeffery Wickens
Keyword(s):  
Membrane Potential ◽  
Projection Neurons ◽  
Tonic Firing ◽  
Short Term Plasticity ◽  
Cholinergic Interneurons ◽  
Significant Events ◽  
Output Neurons ◽  
Potential Activity ◽  
Membrane Potential Fluctuations

The cholinergic interneurons (CINs) of the striatum are crucial for normal motor and behavioral functions of the basal ganglia. Striatal CINs exhibit tonic firing punctuated by distinct pauses. Pauses occur in response to motivationally significant events, but their function is unknown. Here we investigated the effects of pauses in CIN firing on spiny projection neurons (SPNs) – the output neurons of the striatum – using in vivo whole cell and juxtacellular recordings in mice. We found that optogenetically-induced pauses in CIN firing inhibited subthreshold membrane potential activity and decreased firing of SPNs. During pauses, SPN membrane potential fluctuations became more hyperpolarized and UP state durations became shorter. In addition, short-term plasticity of corticostriatal inputs was decreased during pauses. Our results indicate that, in vivo, the net effect of the pause in CIN firing on SPNs activity is inhibition and provide a novel mechanism for cholinergic control of striatal output.

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