Effect of gasotransmitters donors (nitrogen oxide, hydrogen sulfide and carbon monoxide) on erythrocytes microrheological properties

2021 ◽  
Author(s):  
А.В. Муравьев ◽  
И.А. Тихомирова ◽  
Е.П. Петроченко ◽  
Ю.А. Малышева ◽  
Н.В. Кислов
Keyword(s):  
Carbon Monoxide ◽  
Hydrogen Sulfide ◽  
Nitrogen Oxide ◽  
Blood Rheology ◽  
Signaling Molecules ◽  
Target Cells ◽  
No Donor ◽  
Sodium Hydrosulfide ◽  
Total Difference ◽  
Transport Potential

Введение. Микрореологические свойства эритроцитов — их деформируемость и агрегация в значительной степени определяют текучесть цельной крови и ее кислородотранспортный потенциал. При этом эритроциты — это клетки-мишени, на которые действуют сигнальные молекулы. Последние могут регуляторно изменять микромеханические свойства эритроцитов и реологию крови в целом. К сигнальным молекулам относятся и газотрансмиттеры (ГТ) — такие соединения, как оксид азота (NO), сульфид водорода (H2S) и монооксид углерода (CO). Доноры ГТ могут быть основой для разработки лекарственных препаратов для коррекции реологических свойств крови. Цель работы: изучение изменений микрореологических характеристик эритроцитов под влиянием доноров трех газотрансмиттеров — NO, H2S и CO. Материалы и методы. Эритроциты и их восстановленные тени инкубировали с нитропруссидом натрия (НПН, 100 мкмоль) — донором NO, гидросульфидом натрия (NaHS, 100 мкмоль) — донором H2S и трикарбонилхлор (глицинат) рутением (II) (CORM-3, 50 мкмоль) — донором СО. После этого регистрировали деформируемость эритроцитов (ИУЭ), их агрегацию (ПАЭ) и вязкость суспензий (ВС). Результаты. Установлено, что под влиянием всех трех доноров ГТ происходят заметные изменения микрореологических характеристик эритроцитов и их теней (в среднем на 13–16%; p < 0,05). Важно заметить, что сдвиги ИУЭ, ПАЭ и ВС под влиянием доноров газотрансмиттеров были сходными по величине, и суммарная разница их микрореологических эффектов на три разных донора ГТ не превышала 3%. Заключение. На основании полученных данных и их анализа можно заключить, что эритроциты качественно и количественно примерно одинаково отвечают на действие трех разных доноров ГТ положительными изменениями их микрореологических характеристик и особенно агрегации, которая снижалась более чем на 30%. Background. Erythrocytes microrheological properties (deformability and aggregation) largely determine the fluidity of whole blood and its oxygen transport potential. At the same time erythrocytes are target cells for signaling molecules acting. The latter can regulate erythrocytes micromechanical properties and blood rheology in general. Signaling molecules also include gasotransmitters (GT) — compounds such as nitrogen oxide (NO), hydrogen sulfide (H2S) and carbon monoxide (CO). GT donors can form the basis of drugs for blood rheology correction. Objectives: to study the changes of erythrocytes microrheological characteristics under the influence of donors of three gasotransmitters — NO, H2S and CO. Materials/Methods. Erythrocytes and their restored shadows were incubated with sodium nitroprusside (SNP, 100 μmol) — NO donor, sodium hydrosulfide (NaHS, 100 μmol) — H2S donor, and with tricarbonylchlor (glycinate) ruthenium (II) (CORM-3, 50 μmol) — CO donor. Thereafter we registered erythrocytes deformability (ED), their aggregation (EA) and suspensions viscosity (SV). Results. All three GT donors noticeably changed microrheological characteristics of erythrocytes and their shadows (on average by 13–16%; p < 0.05). It’s important that the shifts of ED, EA, and SV under the influence of gasotransmitter donors were similar in magnitude, and the total difference in their microrheological effects on three different GT donors did not exceed 3%. Conclusions. Erythrocytes qualitatively and quantitatively approximately equally responded to the action of three different GT donors by positive changes in their microrheological characteristics and especially by aggregation that decreased by more than 30%.

Hemorheological parameters in individuals with different levels of oxygen body supply: the effect of nitric oxide and hydrogen sulfi de on erythrocyte microrheological characteristics.

2021 ◽  
Author(s):  
А.В. Муравьев ◽  
П.В. Михайлов ◽  
В.В. Зинчук ◽  
И.А. Тихомирова ◽  
Р.С. Остроумов
Keyword(s):  
Nitric Oxide ◽  
Blood Flow ◽  
Oxygen Transport ◽  
Blood Viscosity ◽  
Oxygen Supply ◽  
No Donor ◽  
Sodium Hydrosulfide ◽  
Positive Effect ◽  
Different Levels ◽  
Transport Potential

Введение. Доставка кислорода в ткани определяется величиной объемного кровотока; он, в свою очередь, зависит от сосудистых и реологических факторов. Снижение вязкости крови (ВК) может способствовать приросту объемного кровотока и повышению эффективности доставки кислорода. Поскольку ВК тесно связана с микрореологическими свойствами эритроцитов, то можно полагать, что их положительные изменения будут способствовать улучшению кислородтранспортной функции крови. Цель исследования: сравнительный анализ гемореологических профилей у лиц с разным уровнем обеспечения организма кислородом и определение роли оксида азота (NO) и сульфида водорода (H2S) в изменениях микрореологических характеристик эритроцитов. Материалы и методы. На основе результатов определения максимального потребления кислорода (МПК) были сформированы 2 группы, в каждой из которых было по 24 практически здоровых мужчины-добровольца в возрасте от 20 до 35 лет: группа 1 – лица с умеренным обеспечением организма кислородом (МПК = 40–50 мл/кг/мин) и группа 2 – лица с относительно высоким его уровнем (МПК = 51–65 мл/кг/мин). Регистрировали параметры гемореологического профиля, напряжение кислорода в коже предплечья (tсрО2), метаболизм оксида азота (по соотношению нитраты/нитриты, NOx). Для исследования влияния газотрансмиттеров (ГТ) на микрореологию эритроцитов их инкубировали с донором NO (нитропруссидом натрия, 100 мкмоль) и донором H2S (гидросульфидом натрия, 100 мкмоль) с последующей регистрацией деформируемости и агрегации эритроцитов. Результаты. У лиц с относительно высоким обеспечением тканей кислородом отмечалась сниженная вязкость крови, ее высокий кислородтранспортный потенциал, эффективная микрореология эритроцитов и их более высокая чувствительность к ГТ при положительном влиянии последних на агрегацию и деформируемость эритроцитов. Заключение. Данные, полученные на моделях микрореологических ответов эритроцитов на доноры двух газотрансмиттеров, позволяют заключить, что, во-первых, эти ГТ, как сигнальные молекулы, положительно влияют на микрореологические характеристики эритроцитов и, следовательно, на их транспортный потенциал, и, во-вторых, эритроциты лиц, имеющих высокий уровень обеспечения организма кислородом, более чувствительны к регуляторному действию газотрансмиттеров, поскольку их микрореологические ответы на доноры были статистически значимо более выраженными. Background. Oxygen delivery to tissues is determined by the volume of blood flow that, in turn, depends on vascular and rheological factors. Blood viscosity (BV) decreasing can promote an increasing of volumetric blood flow and provide more efficient oxygen transport. Since BV depends on the erythrocyte microrheological properties it can be assumed that their positive changes will contribute to better oxygen transport. Objectives: to investigate hemorheological profiles in individuals with different levels of body oxygen supply and the role of nitrogen oxide (NO) and hydrogen sulfide (H2S) in changes of the erythrocyte microrheological characteristics. Patients/Methods. Based on the determination of maximum oxygen consumption (VO2max), 2 groups were formed, each of which consisted of 24 practically healthy male volunteers aged 20–35 years: group 1 – persons with moderate body oxygen supply (VO2max = 40–50 ml/kg/min) and group 2 – persons with a relatively high body oxygen supply (VO2max = 51–65 ml/kg/min). Hemorheological profi le parameters, oxygen tension in the forearm skin (tcpO2), and nitric oxide metabolism by the ratio of nitrates/nitrites (NOx) were recorded. To study the effect of gasotransmitters (GTs) on erythrocyte microrheology, they were incubated with NO donor (sodium nitroprusside, 100 μmol) and H2S donor (sodium hydrosulfide, 100 μmol), and erythrocytes deformability and aggregation were registered. Results. Individuals with a relatively high oxygen supply of tissues showed a reduced blood viscosity, high blood oxygen transport potential, an effective microrheology of erythrocytes and their higher sensitivity to GTs with their positive effect on erythrocytes aggregation and deformability. Conclusions. The obtained data on models of erythrocyte microrheological responses to donors of two gasotransmitters allow us to conclude that, firstly, these GTs, as signaling molecules, have a positive effect on the erythrocyte microrheological characteristics and, consequently, on their transport potential, and, secondly, erythrocytes from individuals with a high level of oxygen body supply are more sensitive to the regulatory action of GTs, because their microrheological responses to donors were statistically significantly more expressed.

scholarly journals Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (Triticum aestivum L.) Plants

Antioxidants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 108
Author(s):  
Noushina Iqbal ◽  
Shahid Umar ◽  
Nafees A. Khan ◽  
Francisco J. Corpas
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Triticum Aestivum ◽  
Hydrogen Sulfide ◽  
Stress Reduction ◽  
Triticum Aestivum L ◽  
No Donor ◽  
Sodium Hydrosulfide ◽  
Gsh Metabolism ◽  
Glutathione Cycle

The involvement of nitric oxide (NO) and hydrogen sulfide (H2S) in countermanding heat-inhibited photosynthetic features were studied in wheat (Triticum aestivum L.). Heat stress (HS) was employed at 40 °C after establishment for 6 h daily, and then plants were allowed to recover at 25 °C and grown for 30 days. Glucose (Glc) content increased under HS and repressed plant photosynthetic ability, but the application of sodium nitroprusside (SNP, as NO donor) either alone or with sodium hydrosulfide (NaHS, as H2S donor) reduced Glc-mediated photosynthetic suppression by enhancing ascorbate-glutathione (AsA-GSH) metabolism and antioxidant system, which reduced oxidative stress with decreased H2O2 and TBARS content. Oxidative stress reduction or inhibiting Glc repression was maximum with combined SNP and NaHS treatment, which was substantiated by 2-4-carboxyphenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) and hypotaurine (HT), scavengers for NO and H2S, respectively. The scavenge of H2S reduced NO-mediated alleviation of HS suggesting of its downstream action in NO-mediated heat-tolerance. However, a simultaneous decrease of both (NO and H2S) led to higher Glc-mediated repression of photosynthesis and oxidative stress in terms of increased H2O2 content that was comparable to HS plants. Thus, NO and H2S cooperate to enhance photosynthesis under HS by reducing H2O2-induced oxidative stress and excess Glc-mediated photosynthetic suppression.

Kinetics of catalytic reduction of nitrogen oxide by carbon monoxide on CuO/Al2O3 catalyst

1983 ◽  
Vol 48 (11) ◽  
pp. 3202-3208 ◽  
Author(s):  
Zdeněk Musil ◽  
Vladimír Pour
Keyword(s):  
Carbon Monoxide ◽  
Nitrogen Oxide ◽  
Rate Equation ◽  
Catalytic Reduction ◽  
Zero Order ◽  
Spectroscopic Measurements ◽  
First Order ◽  
Ir Spectroscopic ◽  
Kinetics Of ◽  
Al2o3 Catalyst

The kinetics of the reduction of nitrogen oxide by carbon monoxide on CuO/Al2O3 catalyst (8.36 mass % CuO) were determined at temperatures between 413 and 473 K. The reaction was found to be first order in NO and zero order in CO. The observed kinetics are consistent with a rate equation derived from a mechanism proposed on the basis of IR spectroscopic measurements.

scholarly journals Hydrogen Sulfide and Carbon Monoxide Protect Gastric Mucosa Compromised by Mild Stress Against Alendronate Injury

2016 ◽  
Vol 61 (11) ◽  
pp. 3176-3189 ◽  
Author(s):  
Marcin Magierowski ◽  
Katarzyna Magierowska ◽  
Jakub Szmyd ◽  
Marcin Surmiak ◽  
Zbigniew Sliwowski ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Hydrogen Sulfide ◽  
Gastric Mucosa ◽  
Mild Stress

scholarly journals Hydrogen Sulfide Increases Nitric Oxide Production and Subsequent S-Nitrosylation in Endothelial Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ping-Ho Chen ◽  
Yaw-Syan Fu ◽  
Yun-Ming Wang ◽  
Kun-Han Yang ◽  
Danny Ling Wang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Hydrogen Sulfide ◽  
Protein Kinase ◽  
Fluorescent Probe ◽  
Acid Methyl Ester ◽  
High Specificity ◽  
Protein S ◽  
No Production ◽  
No Donor

Hydrogen sulfide (H2S) and nitric oxide (NO), two endogenous gaseous molecules in endothelial cells, got increased attention with respect to their protective roles in the cardiovascular system. However, the details of the signaling pathways between H2S and NO in endothelia cells remain unclear. In this study, a treatment with NaHS profoundly increased the expression and the activity of endothelial nitric oxide synthase. Elevated gaseous NO levels were observed by a novel and specific fluorescent probe, 5-amino-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid methyl ester (FA-OMe), and quantified by flow cytometry. Further study indicated an increase of upstream regulator for eNOS activation, AMP-activated protein kinase (AMPK), and protein kinase B (Akt). By using a biotin switch, the level of NO-mediated protein S-nitrosylation was also enhanced. However, with the addition of the NO donor, NOC-18, the expressions of cystathionine-γ-lyase, cystathionine-β-synthase, and 3-mercaptopyruvate sulfurtransferase were not changed. The level of H2S was also monitored by a new designed fluorescent probe, 4-nitro-7-thiocyanatobenz-2-oxa-1,3-diazole (NBD-SCN) with high specificity. Therefore, NO did not reciprocally increase the expression of H2S-generating enzymes and the H2S level. The present study provides an integrated insight of cellular responses to H2S and NO from protein expression to gaseous molecule generation, which indicates the upstream role of H2S in modulating NO production and protein S-nitrosylation.

Hydrogen sulfide reduces serum triglyceride by activating liver autophagy via the AMPK-mTOR pathway

2015 ◽  
Vol 309 (11) ◽  
pp. E925-E935 ◽  
Author(s):  
Li Sun ◽  
Song Zhang ◽  
Chengyuan Yu ◽  
Zhenwei Pan ◽  
Yang Liu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
High Fat Diet ◽  
Serum Triglyceride ◽  
Mtor Pathway ◽  
Autophagic Flux ◽  
High Fat ◽  
Sodium Hydrosulfide ◽  
Metabolism Inhibition ◽  
Qualitative Effect ◽  
First Time

Autophagy plays an important role in liver triglyceride (TG) metabolism. Inhibition of autophagy could reduce the clearance of TG in the liver. Hydrogen sulfide (H2S) is a potent stimulator of autophagic flux. Recent studies showed H2S is protective against hypertriglyceridemia (HTG) and noalcoholic fatty liver disease (NAFLD), while the mechanism remains to be explored. Here, we tested the hypothesis that H2S reduces serum TG level and ameliorates NAFLD by stimulating liver autophagic flux by the AMPK-mTOR pathway. The level of serum H2S in patients with HTG was lower than that of control subjects. Sodium hydrosulfide (NaHS, H2S donor) markedly reduced serum TG levels of male C57BL/6 mice fed a high-fat diet (HFD), which was abolished by coadministration of chloroquine (CQ), an inhibitor of autophagic flux. In HFD mice, administration of NaSH increased the LC3BII-to-LC3BI ratio and decreased the p62 protein level. Meanwhile, NaSH increased the phosphorylation of AMPK and thus reduced the phosphorylation of mTOR in a Western blot study. In cultured LO2 cells, high-fat treatment reduced the ratio of LC3BII to LC3BI and the phosphorylation of AMPK, which were reversed by the coadministration of NaSH. Knockdown of AMPK by siRNA in LO2 cells blocked the autophagic enhancing effects of NaSH. The same qualitative effect was observed in AMPKα2−/− mice. These results for the first time demonstrated that H2S could reduce serum TG level and ameliorate NAFLD by activating liver autophagy via the AMPK-mTOR pathway.

NO, CO and H2S based pharmaceuticals in the mission of vision (eye health): a comprehensive review

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Jan Mohammad Mir ◽  
Ram Charitra Maurya ◽  
Mohd Washid Khan
Keyword(s):  
Nitric Oxide ◽  
Carbon Monoxide ◽  
Human Physiology ◽  
Hydrogen Sulfide ◽  
The Other ◽  
Normal Functioning ◽  
Comprehensive Review ◽  
Molecular Systems ◽  
Eye Health ◽  
Highly Sensitive

Abstract A set of well defined signaling molecules responsible for normal functioning of human physiology including nitric oxide along with carbon monoxide and hydrogen sulphide are referred as “gasotransmitters”. Due to their involvement in almost every system of a human body, the care of highly sensitive organs using these molecules as drugs represents highly fascinating area of research. In connection with these interesting aspects, the applied aspects of these gaseous molecules in maintaining healthy eye and vision have been targeted in this review. Several examples of eye-droppers including NORMs like latanoprost and nipradiol, CORMs like CORM-3 and CORM-A1, and Hydrogen sulfide releasing system like GYY4137 have been discussed in this context. Therefore the relation of these trio-gasotransmitters with the ophthalmic homeostasis on one hand, and de-infecting role on the other hand has been mainly highlighted. Some molecular systems capable of mimicking gasotransmitter action have also been introduced in connection with the titled theme.

scholarly journals COMBUSTION PERFORMANCE OF SYNGAS FROM BIOMASS WASTE IN GAS BURNER SYSTEM/PRESTASI PEMBAKARAN SYNGAS DARI SISA BIOJISIM DALAM SISTEM PEMBAKAR GAS

2018 ◽  
Vol 80 (5) ◽  
Author(s):  
Mohd Oryza Mohd Mokhtar ◽  
Mohammad Nazri Mohd Ja’afar ◽  
Mustafa Yusoff ◽  
Mazlan Said ◽  
Muhammad Roslan Rahim ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Sulfur Dioxide ◽  
Nitrogen Oxide ◽  
Combustion Temperature ◽  
Fossil Fuel ◽  
Biomass Waste ◽  
Gas Combustion ◽  
Combustion Performance ◽  
Positive Effects ◽  
Gas Burner

Syngas from biomass residues is an alternative fuel to address the ever-increasing fossil fuel supply problem and the issue of releasing toxic gases from the fossil fuel burning process. Syngas is also a renewable fuel and features environmentally friendly fuel. This study was conducted to investigate the performance of the syngas produced from oil palm shells (PKS) using fluidized bed gasifier. In this study, the produced syngas was tested for its combustion performance from the aspect of gas combustion temperature and resulting emission concentrations such as nitrogen oxide (NOx), carbon monoxide (CO) and sulfur dioxide (SO2). The resulting syngas was studied at different ratio of air velocities to fuels. From the test, the ratio of velocity of air to fuel affects the gas combustion temperature and emission emission concentration. By increasing the air velocity to fuel ratio during the gasification process produces more positive effects primarily in improving the temperature of the gas burner combustion and reducing carbon monoxide (CO) emissions. However, the concentration of sulfur dioxide release (SO2) and nitrogen oxide (NOx) increase.

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