The role of hemorheological disorders in the pathogenesis of hemorrhagic pancreonecrosis

2021 ◽  
Author(s):  
Н.П. Александрова ◽  
В.И. Карандашов ◽  
А.В. Варданян
Keyword(s):  
Sialic Acid ◽  
Shear Rate ◽  
Electrophoretic Mobility ◽  
Blood Viscosity ◽  
Proteolytic Enzymes ◽  
Biologically Active ◽  
Erythrocyte Aggregation ◽  
Control Group ◽  
Mechanical Resistance

Введение. Вопросы патогенеза острого панкреатита и панкреонекроза до настоящего времени остаются в центре внимания исследователей и клиницистов. До сих пор до конца не выяснена роль изменений в системе гемостаза и гемореологических нарушений в развитии этого заболевания. Цель исследования: установить роль гемореологических нарушений в патогенезе геморрагического панкреонекроза и изучить специфику механизма этих расстройств. Материалы и методы. Обследовано 29 пациентов с геморрагическим панкреонекрозом (12 женщин и 17 мужчин) в возрасте от 23 до 60 лет. Исследовали вязкость крови, показатель гематокрита, количество эритроцитов и их диаметр, агрегацию, электрофоретическую подвижность, деформируемость и механическую резистентность эритроцитов, белковый состав плазмы, содержание сиаловой кислоты в плазме и в эритроцитах, параметры липидного обмена, содержание кальция и фибриногена в крови,фибринолитическую активность крови и агрегационную активность тромбоцитов, гемокоагуляционная активность исследована методом тромбоэластографии. Для определения нормальных значений исследованных показателей было обследовано 15 практически здоровых лиц (7 женщин и 8 мужчин). Результаты. У больных панкреонекрозом самым грубым нарушениям подвергаются эритроциты: их механическая резистентность снижалась в 2 раза, объем увеличивался на 18,7%, деформируемость падала на 43,8%, количество снижалось на 8,75%, показатель гематокрита при этом оставался на уровне нормальных значений по причине увеличенного объема (сферичности) клеток; в 1,8 раза возрастала агрегация эритроцитов. Вязкость крови при скорости сдвига 1 c–1 увеличивалась в 3,3 раза, а при скорости сдвига 150 c–1 — в 1,58 раза по сравнению с нормой. Причиной повышения агрегации эритроцитов являлось снижение их электрофоретической подвижности на 35,9% из-за десиализации их мембран: концентрация сиаловой кислоты в клеточных мембранах была снижена на 20,8%, а содержание конъюгированной сиаловой кислоты в плазме увеличено в 2,25 раза по сравнению с нормальными значениями. Заключение. Гемореологические расстройства, которые возникают первоначально у больных геморрагическим панкреонекрозом как результат некротических изменений поджелудочной железы, с определенного, довольно раннего этапа сами становятся фактором патогенеза данного заболевания. Доминирующим фактором прогрессивного увеличения вязкости крови у больных панкреонекрозом является нарушение морфофункциональных и физико-химических свой ств эритроцитов на фоне высокой активности протеолитических ферментов, биологически активных аминов и крайней степени токсемии. Background. The pathogenesis of acute pancreatitis and pancreonecrosis is still the focus of researchers and clinicians. The role of hemorheological disorders in these diseases remain uncertain until now. Objectives: to define the role of hemorheological disorders in the pathogenesis of hemorrhagic pancreonecrosis and to study the specifics of the mechanism of these disorders. Patients/Methods. This study included 29 patients (12 women and 17 men, age of 23 to 60 years old) with hemorrhagic pancreatic necrosis. We examined blood viscosity, hematocrit and some erythrocyte properties as count, diameter, aggregation, electrophoretic mobility, deformability and mechanical resistance; other investigated parameters were plasma protein composition, plasma and erythrocytes sialic acid concentrations, lipids, total calcium and fibrinogen concentrations, blood fibrinolytic activity, platelets aggregation activity; total hemocoagulation activity was studied with thromboelastography. Control group contained 15 practically healthy individuals (7 women and 8 men). Results. Expressed disturbances of blood rheological properties, mostly in erythrocytes were detected in patients with pancreonecrosis. Red blood cells (RBC) showed 2-times decreasing of mechanical resistance, of their volume by 18.7%, of deformability by 43.8%, of count by 8.75%. Hematocrit remained normal level due to RBC increased volume (sphericity). RBC aggregation had been increased by 1.8 times. Blood viscosity at the shear rate of 1 s–1 was increased by 3.3 times and at the shear rate of 150 s–1 by 1.58 times. Raised erythrocyte aggregation was caused by a decrease of RBC electrophoretic mobility of 35.9%. Sialic acid concentration in RBC membranes was lower of 20.8% whereas conjugated sialic acid in plasma showed increasing by 2.25 times. Conclusions. RBC morphofunctional and physicochemical disturbances cause the increase in blood viscosity in patients with pancreonecrosis. It is distinguishing feature of hemorheological disorders in hemorrhagic pancreonecrosis developing, seems, due to high activity of proteolytic enzymes and biologically active amines. Of particular importance in hemorrhagic pancreonecrosis belong to platelet involving into intravascular coagulation.

Influence of Postoperative Patient-Controlled Analgesia on Hemorheology in Patients Undergoing Hip Arthroplasty

2019 ◽  
Vol 158 (01) ◽  
pp. 41-45
Author(s):  
Yiping Bai ◽  
Liqun Mo ◽  
Liming Luan ◽  
Daiying Zhang
Keyword(s):  
Shear Rate ◽  
Hip Arthroplasty ◽  
Blood Viscosity ◽  
Whole Blood ◽  
Plasma Viscosity ◽  
Erythrocyte Aggregation ◽  
Control Group ◽  
Patient Controlled Analgesia ◽  
Noninvasive Blood Pressure ◽  
Whole Blood Viscosity

Abstract Objective To test the hypothesis that patient-controlled analgesia (PCA) contributes to improvement of hemorheology in patients undergoing hip arthroplasty. Methods 120 patients, aged 60 – 75 years old, undergoing hip arthroplasty under spinal anesthesia, were randomly divided into group PCA (n = 60) and control group (n = 60). Patients in PCA group received PCA in postoperative 3 days. Blood samples from the median cubital vein were collected at five time points: before anesthesia (T1), after surgery (T2), 6 h after surgery (T3), 24 h after surgery (T4), 48 h after surgery (T5). Hemorheological parameters were measured, including whole blood viscosity at a high shear rate (Hηb), whole blood viscosity at a low shear rate (Lηb), reduced viscosity (ηr), plasma viscosity (ηp), hematocrit (Hct), erythrocyte aggregation index(EAI) and erythrocyte deformation index (EDI). Noninvasive blood pressure and heart rate at T1-5 and pain scoring of visual analogue scale (VAS) score at T2-5 were recorded. Results (1) Compared with T1, Hηb, Lηb, ηp, ηr decreased significantly at T3–5 with EAI decreased significantly at T5 in group PCA (p < 0.05), EDI increased significantly at T5 in group C (p < 0.05). (2) Compared with group C, Hηb, Lηb, ηp, ηr, EAI decreased significantly at T5 with Lηb concurrently decreased at T4 in group PCA (p < 0.05). Conclusion Postoperative pain may increase blood viscosity in patients undergoing hip arthroplasty, mainly via plasma viscosity, erythrocyte aggregation and rigidity, and which could be improved by postoperative PCA.

scholarly journals Microcirculatory injuries of patients with chronic generalized periodontitis and their correction with EHF-therapy

2003 ◽  
Vol 2 (2) ◽  
pp. 99-103
Author(s):  
V. F. Kiritchuk ◽  
A. V. Lepilin ◽  
I. P. Apalkov ◽  
N. L. Yerokina
Keyword(s):  
Rheological Properties ◽  
Blood Viscosity ◽  
Oral Fluid ◽  
Anticoagulant Activity ◽  
Wave Length ◽  
Biologically Active ◽  
Erythrocyte Aggregation ◽  
Time Activity ◽  
Hemostasis System ◽  
Fluid Ability

The aim is the study of EHF-therapy (extremely high frequency therapy) potential for the correction of microcirculatory injuries of patients with chronic generalized periodontitis in postsurgery period: in blood rheological properties and hemostasis system, change of oral fluid ability to coagulation. 42 patients have been examined and treated, including a complex periodontium examination: parodontal recess depth, stomatorrhagia at probing and gum capillary resistance. There have been determined a blood viscosity, erythrocyte aggregation, erythrocyte membrane deformability with rotation viscometer ABR-2; as well as activated partial thromboplastin time, prothrombin time, activity with coagulometer «Sola-Cey 2120» (Byelorussia) using reagents of «Tyekhnologiya-standart» company (Barnaul, Russia). An apparatus «Yavj-1» with wave-length of 5,6 mm with the exposure to biologically active face points (cv-26, cv-27, st-7, st-8) has been used for EHF-therapy. There has been demonstrated that patients with chronic generalized periodontitis had blood rheological and coagulation properties disturbed, that accompanied with the increase of blood viscosity, erythrocyte aggregation, tissue prothrombinase activity and the decrease of blood antithrombin and anticoagulant activity. Patients oral fluid components intensify the disturbances in blood rheological properties due to erythrocyte aggregation increase, and at the same time increase the blood coagulation potential. EHF-therapy recovers the blood rheological properties (its viscosity) more effectively, erythrocyte ability to aggregation and hemostasis system factors in comparison with the traditional treatment.

scholarly journals Activity of Proteolytic Enzymes and Level of Cystatin C in the Peripartum Period

2016 ◽  
Vol 2016 ◽  
pp. 1-5
Author(s):  
Anna Cyganek ◽  
Aleksandra Wyczalkowska-Tomasik ◽  
Patrycja Jarmuzek ◽  
Barbara Grzechocinska ◽  
Zoulikha Jabiry-Zieniewicz ◽  
...  
Keyword(s):  
Cystatin C ◽  
Cathepsin B ◽  
Proteolytic Enzymes ◽  
Late Pregnancy ◽  
Control Group ◽  
Study Group ◽  
Serum Cystatin C ◽  
Serum Cystatin ◽  
Peripartum Period

Objectives. The aim of the study was to evaluate the activity of cathepsin B, collagenases, trypsin, and plasmin and concentration of cystatin C in serum of healthy pregnant women in peripartum period.Study Design. The study group included 45 women in uncomplicated pregnancies. Blood samples were collected in four time points. Enzyme activity was measured by spectrofluorometric method. The level of cystatin C was measured using immunonephelometric method.Results. Mean activity of cathepsin B and the level of serum cystatin C were significantly higher in the study group. Collagenase activity was significantly lower in the study group than the control group. No differences in collagenase, plasmin, and trypsin activity on each day of the peripartum period were found.Conclusion. High activity of cathepsin B and increased level of cystatin C are typical for women in late pregnancy. Those levels significantly decrease after delivery which can be associated with potential role of those markers in placental separation. The insignificant changes of cystatin C level in the peripartum period seem to exclude the possibility of using cystatin C as a marker for renal insufficiency in the peripartum period but additional research is necessary to investigate the matter further.

scholarly journals Role of sialic acid in erythrocyte survival

Blood ◽  
1975 ◽  
Vol 45 (1) ◽  
pp. 11-20 ◽  
Author(s):  
JR Durocher ◽  
RC Payne ◽  
ME Conrad
Keyword(s):  
Sialic Acid ◽  
Surface Charge ◽  
Electrophoretic Mobility ◽  
Polyacrylamide Gels ◽  
Membrane Glycoproteins ◽  
Atp Levels ◽  
Erythrocyte Survival ◽  
Pas Staining ◽  
Rapid Clearance

The role of membrane sialic acid in erythrocyte survival is unclear, although there is evidence for a reduction in sialic acid and surface charge in older erythrocytes. We reduced the surface charge of human, rat, and rabbit erythrocytes by removing sialic acid with neuraminidase.Reduction in sialic acid correlated with decreases in electrophoretic mobility and loss of PAS staining of membrane glycoproteins on polyacrylamide gels. No changes in ATP levels or deformability were found. 51Cr erythrocyte survivals in rats and rabbits showed rapid clearance of desialylated erythrocytes with sequestration by the liver.These results suggest that reduction in erythrocyte sialic acid is a mechanism of erythrocyte destruction and may be important in erythrocyte senescence.

Non-Pathogenic Anti-Heparin/PF4 Antibodies Generated by Low Molecular Weight Heparins Mediate Procoagulant Effects.

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 1091-1091
Author(s):  
Jeanine M. Walenga ◽  
Debra Hoppensteadt ◽  
Evangelos Litinas ◽  
Harry L. Messmore ◽  
Bruce E Lewis ◽  
...  
Keyword(s):  
Thrombin Generation ◽  
Conflicts Of Interest ◽  
Biologically Active ◽  
Control Group ◽  
Heparin Group ◽  
Thrombin Generation Assay ◽  
Enoxaparin Group ◽  
Pre Treatment ◽  
Inhibition Of Thrombin

Abstract Abstract 1091 Introduction: While the incidence of symptomatic heparin-induced thrombocytopenia (HIT) is relatively low with the use of low molecular weights heparins (LMWHs), these agents do generate anti-heparin/PF4 antibodies in 10–20% of treated patients. Dosage, duration, and the pathologic predisposition of the patient influence the quantitative and qualitative nature of these antibodies. It has been suggested that these non-pathogenic antibodies (NPAs) which do not produce symptomatic HIT may, nevertheless, be biologically active and mediate thrombogenic responses. The overall pathophysiologic role of NPAs is unknown at this time. Hypothesis: NPAs generated by LMWHs cause coagulation activation and compromise the anticoagulant effects of the administered LMWH. Study Design: Blood plasma samples collected at baseline and day 10 from patients enrolled in orthopedic surgery clinical trials of LMWHs for the prophylactic management of deep vein thrombosis (Lovenox enoxaparin, sanofi-aventis, n=352; Clivarin reviparin, Abbott, n=380) were retrospectively screened for the presence of anti-heparin/PF4 antibodies using the GTI ELISA method (Waukesha, WI). Positive samples were tested by the 14C-SRA to determine if the antibodies were capable of functionally activating platelets. Both ELISA positive and negative samples were evaluated in an assay of thrombin generation (Technothrombin TGA kit, diaPharma, West Chester, OH). Result: In the enoxaparin study, the baseline pre-treatment samples only showed one patient in the heparin control group to be positive by ELISA. On day 10, 11 of 175 (6.3%) enoxaparin patients had a positive ELISA response, whereas 22 of 177 (12.4%) heparin patients were ELISA positive. None of the samples were 14C-SRA positive. In the thrombin generation assay, the ELISA positive samples showed a lesser inhibition of thrombin generation for both the enoxaparin and heparin groups (270 ± 27 nM TGA enoxaparin group; 220 ± 21 nM TGA heparin group) compared to the thrombin generation response of the ELISA negative samples (190 ± 18 nM TGA enoxaparin group; 160 ± 20 nM TGA heparin group). In the reviparin study, none of the patients were ELISA positive at baseline. On day 10, in the reviparin group 19 of 200 (9.5%) patients were ELISA positive, whereas 28 of 180 (15.6%) heparin control patients had a positive ELISA titer. None of the samples were 14C-SRA positive. In comparison to the baseline (pre-treatment), both the reviparin and heparin treated patients showed an inhibition of thrombin generation (410 ± 27 nM TGA baseline vs 180–290 nM with treatment). However, consistent with the above study, those samples that were ELISA antibody positive showed a lesser inhibition of thrombin generation (240 ± 21 nM TGA reviparin group; 210 ± 16 nM TGA heparin group) in comparison to the ELISA negative samples (190 ± 12 nM TGA reviparin group; 180 ± 14 nM TGA heparin group). Interestingly, the D-dimer levels were found to be higher in the ELISA positive samples in all groups for both studies (p<0.05). Conclusion: These studies suggest a potential pathologic role of NPAs. The results of the thrombin generation studies strongly suggest that the generation of NPAs may result in a reduction of the antithrombotic potential of both LMWH and heparin in treated patients. While the exact mechanism of this process is not clear, dosage adjustment may be useful in those patients who generate NPAs. Disclosures: No relevant conflicts of interest to declare.

ROLE OF GALACTOSE IN THE ANTIGENIC PROPERTIES OF THYROGLOBULIN

1976 ◽  
Vol 83 (1) ◽  
pp. 93-98 ◽  
Author(s):  
F. Monaco ◽  
M. Andreoli
Keyword(s):  
Sialic Acid ◽  
Thyroid Nodule ◽  
Electrophoretic Mobility ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Human Thyroid ◽  
Antigenic Properties ◽  
Beta Galactosidase

ABSTRACT Thyroglobulins (TG) from a "hot" human thyroid nodule and from Fisher rats have been purified and the effects of progressive removal of sialic acid and galactose on the immunoreactive properties of the proteins were studied. Terminal sialic acid and galactose were released by stepwise hydrolysis with neuraminidase and beta-galactosidase. Agalacto-TG shows a slower electrophoretic mobility than native TG, but in polyacrylamide gel electrophoresis and immunoelectrophoresis it migrates in the same position as asialo-TG. In immunodiffusion agalacto-TG forms a spur with native TG and asialo-TG when tested against anti 19S native TG or anti-asialo-TG sera. It is thus shown that galactose in the terminal environment of the oligosaccharide chains of thyroglobulin is essential for the structural groups involved in the antigenic properties of thyroglobulin.

Haemorheological Proælein Chronic Venous Insufæciency

1999 ◽  
Vol 14 (3) ◽  
pp. 105-110
Author(s):  
G. Azaceta ◽  
S. Romero ◽  
C. Lorente ◽  
J. A. Moreno ◽  
T. Olave ◽  
...  
Keyword(s):  
Shear Rate ◽  
Blood Viscosity ◽  
Chronic Venous Insufficiency ◽  
Strong Influence ◽  
Venous Insufficiency ◽  
Stage Ii ◽  
Stage Iii ◽  
Erythrocyte Aggregation ◽  
High Shear ◽  
Advanced Stages

Background: In recent years, pathophysiological studies and treatment approaches of chronic venous insufficiency (CVI) have focused on haemorheology. Objective: To analyse erythrocyte aggregation (EA) and blood viscosity (BV) in mild and severe stages of CVI. Methods: In 147 patients (three severity stages), EA was measured with a photometric aggregometer, and BV with a cone-plate viscosimeter. Patients with concomitant pathologies affecting haemorheology were excluded. Results: EA was higher in patients (mean EA 10M: 16.4 vs 14.5 control), increasing progressively with the evolution of CVI ( p<0.001). Greatest differences were found for stage III (mean EA 10M: 17.6) vs stage II (15.7) and stage 0 (14.5) respectively ( p<0.001), but rheological abnormalities exist in early grades (I: 16.7) ( p<0.01). Fibrinogen and age had a strong influence on EA ( p<0.003 and p<0.001 respectively). When a covariance analysis avoided their effect, significant global differences between CVI groups persisted. BV at high shear rate was increased at advanced stages (III: mean 3.3 centipoise (cp); I: 3.1 cp) ( p<0.05). Conclusions: We found progressive impairment of the haemorheological profile with the worsening stages of CVI, related to age, severity stage and fibrinogen. Rheological impairment is likely to play a part in the pathophysiology of CVI, and perhaps may be useful in its management.

scholarly journals The Role of Biologically Active Aqueous Humor Molecules of the Anterior Chamber and Lacrimal Fluid in the Implementation of the Hypotensive Effect of Non-Penetrating Deep Sclerectomy

2021 ◽  
Vol 6 (2) ◽  
pp. 126-132
Author(s):  
N. V. Volkova ◽  
Ju. V. Malysheva ◽  
T. N. Iureva ◽  
S. I. Kolesnikov
Keyword(s):  
Anterior Chamber ◽  
Aqueous Humor ◽  
Hypotensive Effect ◽  
Biologically Active ◽  
Control Group ◽  
Formation Mechanisms ◽  
Deep Sclerectomy ◽  
Lacrimal Fluid ◽  
Aqueous Humor Outflow

To date, the factors affecting the course of the reparative process after non-penetrating deep sclerectomy (NPDS) have not been fully determined. There is no systematic information about the regulatory role of the cytokines TGF-β, IL-6, IL-8 and MMP-9, VEGF A 121 and 165 in the formation mechanisms of the newly created pathways consistency of intraocular aqueous humor outflow.Purpose. To determine possible ways of impact of biologically active aqueous humor molecules of the anterior chamber and lacrimal fluid on the hypotensive effect of non-penetrating deep sclerectomy.Methods. A prospective study of 65 patients with open-angle glaucoma before and 12 months after NPDS and 22 patients without eye hydrodynamic disorders with the determination of the initial concentrations of biologically active molecules in the lacrimal fluid and aqueous humor of the anterior chamber. Twelve months after NPDS all patients were divided into three groups, depending on the hypotensive effect of the operation, according to the criteria.Results. Multivariate discriminant analysis showed the greatest inter-group differences, calculated by the square of the Mahalanobis distance, between group 3 with no hypotensive effect of NPDS and the control group (R2 = 8.48, p = 0.001). The most informative features that determine the differences between the 4 groups in the total population, calculated according to the Fischer F-test, were MMP-9 (F = 14.7, p = 0.001) and TGF-β (F = 7.08, p = 0.001) in the aqueous humor of the anterior chamber. In pairwise comparison of groups 1 and 2, the maximum level of significance according to the F-criterion was characteristic of the level of tear IL-6 (F = 21.25, p = 0.001), with approximately equal degree – IL-8 (F = 7.85, p = 0.001) and VEGF (F = 7.12, p = 0.001), to a lesser extent TGF of aqueous humor (F = 4.43, p = 0.001) and MMR-9 (F = 2.23, p = 0.001). Between groups 1–3, the maximum differences according to the Fisher criterion were observed in the IL-8 (F = 20.99, p = 0.001), TGF (F = 8.75, p = 0.001) and to a lesser extent – TGF (F = 5.83, p = 0.001).Conclusion. The analysis of the obtained data showed the decisive role of the imbalance of proinflammatory cytokines, signaling proteins with prolymphoangiogenic activity, and MMP-9 in the aqueous humor of the anterior chamber, as well as in the initial state of the lacrimal fluid in the postoperative healing processes after NPDS. 

Blood Viscosity and Haemostasis in the Nephrotic Syndrome

1983 ◽  
Vol 49 (03) ◽  
pp. 155-157 ◽  
Author(s):  
E McGinley ◽  
G D O Lowe ◽  
M Boulton-Jones ◽  
C D Forbes ◽  
C R M Prentice
Keyword(s):  
Nephrotic Syndrome ◽  
Shear Rate ◽  
Blood Viscosity ◽  
Degradation Products ◽  
Smoking Habit ◽  
Vascular Complications ◽  
Plasma Viscosity ◽  
Control Group ◽  
Fibrin Degradation Products ◽  
Activity Factor

SummaryBlood viscosity and its major determinants (haematocrit, plasma viscosity and fibrinogen) as well as several haemostatic variables were measured in 21 patients with the nephrotic syndrome, and 21 controls matched for age, sex, smoking habit and serum creatinine. Blood viscosity was significantly increased in the nephrotic group, measured at a low shear rate (mean increase 41%, p<0.01) and at a high shear rate (mean increase 25%, p <0.01). Haematocrit was not significantly increased, but plasma viscosity was significantly higher (p <0.01), associated with increased plasma macroglobulins especially fibrinogen, which was increased to double the plasma concentration of the control group (p <0.01). Nephrotic subjects also had increased plasma levels of a2-macroglobulin, factor VIII activity, factor VIC antigen and beta-thromboglobulin; differences in antithrombin ID, fibrin degradation products, plasminogen, and platelet count were not significant.We suggest that increased blood and plasma viscosity may play a role in the vascular complications of the nephrotic syndrome.

Influence of Sialic Acid on Erythrocyte Aggregation in Hypercholesterolemi

1996 ◽  
Vol 76 (06) ◽  
pp. 0944-0949 ◽  
Author(s):  
A Hadengue ◽  
S M Razavian ◽  
M Del-Pino ◽  
A Simon ◽  
J Levenson
Keyword(s):  
Sialic Acid ◽  
Shear Rate ◽  
Acid Content ◽  
Erythrocyte Aggregation ◽  
Total Serum ◽  
Autologous Plasma ◽  
Sialic Acid Content ◽  
Aggregation Index ◽  
Repulsive Forces ◽  
Rate Threshold

SummaryThe respective role of adhesive forces induced by fibrinogen and repulsive forces induced by erythrocyte sialic acid content on erythrocyte aggregation, was investigated in hypercholesterolemic and control subjects. Aggregation index (AI) and disaggregation shear rate threshold (³t) were determined in the presence of either autologous plasma or dextran. Compared with controls, fibrinogen (p <0.001) and aggregation parameters (AI p <0.01; ³t p <0.01) were higher in hypercholester-olemics while erythrocyte sialic acid content (p <0.001) was lower; in addition total serum sialic acid was increased (p <0.01). The aggregation properties of erythrocytes, independent of plasma environment using dextran as a bridging macromolecule, showed an enhanced disaggregation shear rate threshold and an inverse relationship with erythrocyte sialic acid content. We conclude that decreased erythrocyte sialic acid content may intensify the effect of fibrinogen on aggregation and disaggregation of erythrocytes and participate in the development of atherothrombotic complications.

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