scholarly journals The Role of Biologically Active Aqueous Humor Molecules of the Anterior Chamber and Lacrimal Fluid in the Implementation of the Hypotensive Effect of Non-Penetrating Deep Sclerectomy

2021 ◽  
Vol 6 (2) ◽  
pp. 126-132
Author(s):  
N. V. Volkova ◽  
Ju. V. Malysheva ◽  
T. N. Iureva ◽  
S. I. Kolesnikov
Keyword(s):  
Anterior Chamber ◽  
Aqueous Humor ◽  
Hypotensive Effect ◽  
Biologically Active ◽  
Control Group ◽  
Formation Mechanisms ◽  
Deep Sclerectomy ◽  
Lacrimal Fluid ◽  
Aqueous Humor Outflow

To date, the factors affecting the course of the reparative process after non-penetrating deep sclerectomy (NPDS) have not been fully determined. There is no systematic information about the regulatory role of the cytokines TGF-β, IL-6, IL-8 and MMP-9, VEGF A 121 and 165 in the formation mechanisms of the newly created pathways consistency of intraocular aqueous humor outflow.Purpose. To determine possible ways of impact of biologically active aqueous humor molecules of the anterior chamber and lacrimal fluid on the hypotensive effect of non-penetrating deep sclerectomy.Methods. A prospective study of 65 patients with open-angle glaucoma before and 12 months after NPDS and 22 patients without eye hydrodynamic disorders with the determination of the initial concentrations of biologically active molecules in the lacrimal fluid and aqueous humor of the anterior chamber. Twelve months after NPDS all patients were divided into three groups, depending on the hypotensive effect of the operation, according to the criteria.Results. Multivariate discriminant analysis showed the greatest inter-group differences, calculated by the square of the Mahalanobis distance, between group 3 with no hypotensive effect of NPDS and the control group (R2 = 8.48, p = 0.001). The most informative features that determine the differences between the 4 groups in the total population, calculated according to the Fischer F-test, were MMP-9 (F = 14.7, p = 0.001) and TGF-β (F = 7.08, p = 0.001) in the aqueous humor of the anterior chamber. In pairwise comparison of groups 1 and 2, the maximum level of significance according to the F-criterion was characteristic of the level of tear IL-6 (F = 21.25, p = 0.001), with approximately equal degree – IL-8 (F = 7.85, p = 0.001) and VEGF (F = 7.12, p = 0.001), to a lesser extent TGF of aqueous humor (F = 4.43, p = 0.001) and MMR-9 (F = 2.23, p = 0.001). Between groups 1–3, the maximum differences according to the Fisher criterion were observed in the IL-8 (F = 20.99, p = 0.001), TGF (F = 8.75, p = 0.001) and to a lesser extent – TGF (F = 5.83, p = 0.001).Conclusion. The analysis of the obtained data showed the decisive role of the imbalance of proinflammatory cytokines, signaling proteins with prolymphoangiogenic activity, and MMP-9 in the aqueous humor of the anterior chamber, as well as in the initial state of the lacrimal fluid in the postoperative healing processes after NPDS. 

scholarly journals Evaluation of aqueous humor and serum clusterin levels in patients with glaucoma

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Fuat Yavrum ◽  
Ufuk Elgin ◽  
Zeynep Adiyaman Kocer ◽  
Vildan Fidanci ◽  
Emine Sen
Keyword(s):  
Anterior Chamber ◽  
Aqueous Humor ◽  
Open Angle Glaucoma ◽  
Control Group ◽  
Posterior Chamber ◽  
Serum Samples ◽  
Chi Square ◽  
New Finding ◽  
High Level ◽  
And Control

Abstract Background To compare the aqueous humor (AH) and the serum clusterin levels of patients with pseudoexfoliation syndrome (PEX), pseudoexfoliation glaucoma (PEXG), and primary open-angle glaucoma (POAG) with each other and with an age- and sex-matched control group. Methods This prospective, cross-sectionalstudy evaluated 92 eyes from 92 adult cases of uncomplicated phacoemulsification and posterior chamber intraocular lens (IOL) implantation. The cases were divided into PEX, PEXG, POAG, and control groups. Serum samples were taken from the antecubital vein just before the surgery, and the AH samples were aspirated at the beginning of the surgery. Kruskal-Wallis H, One-way ANOVA, Mann-Whitney U with Bonferroni correction and Chi-Square tests were used for statistical analysis. Results The serum clusterin levels were the highest in the PEXG group, but no statistically significant differences were observed between the groups (p=0.633). The mean AH clusterin levels were 286.79±29.64 μg/mL in the PEXG group, 263.92±31.70 μg/mL in the PEX group, 272.59±49.71 μg/mL in the POAG group, and 193.50±62.38 μg/mL in the control group (p< 0.001). This came out to be 1.48 times increase for the PEXG group, 1.36 for the PEX group, and 1.41 for the POAG group when compared with the control subjects. Conclusions A higher level of clusterin in the anterior chamber was found to be associated with PEX and PEXG. In addition, a high level of anterior chamber clusterin in POAG, which is a new finding, showed that this molecule might be important not only in pseudoexfoliation, but also other types of glaucoma like POAG.

The role of hemorheological disorders in the pathogenesis of hemorrhagic pancreonecrosis

2021 ◽  
Author(s):  
Н.П. Александрова ◽  
В.И. Карандашов ◽  
А.В. Варданян
Keyword(s):  
Sialic Acid ◽  
Shear Rate ◽  
Electrophoretic Mobility ◽  
Blood Viscosity ◽  
Proteolytic Enzymes ◽  
Biologically Active ◽  
Erythrocyte Aggregation ◽  
Control Group ◽  
Mechanical Resistance

Введение. Вопросы патогенеза острого панкреатита и панкреонекроза до настоящего времени остаются в центре внимания исследователей и клиницистов. До сих пор до конца не выяснена роль изменений в системе гемостаза и гемореологических нарушений в развитии этого заболевания. Цель исследования: установить роль гемореологических нарушений в патогенезе геморрагического панкреонекроза и изучить специфику механизма этих расстройств. Материалы и методы. Обследовано 29 пациентов с геморрагическим панкреонекрозом (12 женщин и 17 мужчин) в возрасте от 23 до 60 лет. Исследовали вязкость крови, показатель гематокрита, количество эритроцитов и их диаметр, агрегацию, электрофоретическую подвижность, деформируемость и механическую резистентность эритроцитов, белковый состав плазмы, содержание сиаловой кислоты в плазме и в эритроцитах, параметры липидного обмена, содержание кальция и фибриногена в крови,фибринолитическую активность крови и агрегационную активность тромбоцитов, гемокоагуляционная активность исследована методом тромбоэластографии. Для определения нормальных значений исследованных показателей было обследовано 15 практически здоровых лиц (7 женщин и 8 мужчин). Результаты. У больных панкреонекрозом самым грубым нарушениям подвергаются эритроциты: их механическая резистентность снижалась в 2 раза, объем увеличивался на 18,7%, деформируемость падала на 43,8%, количество снижалось на 8,75%, показатель гематокрита при этом оставался на уровне нормальных значений по причине увеличенного объема (сферичности) клеток; в 1,8 раза возрастала агрегация эритроцитов. Вязкость крови при скорости сдвига 1 c–1 увеличивалась в 3,3 раза, а при скорости сдвига 150 c–1 — в 1,58 раза по сравнению с нормой. Причиной повышения агрегации эритроцитов являлось снижение их электрофоретической подвижности на 35,9% из-за десиализации их мембран: концентрация сиаловой кислоты в клеточных мембранах была снижена на 20,8%, а содержание конъюгированной сиаловой кислоты в плазме увеличено в 2,25 раза по сравнению с нормальными значениями. Заключение. Гемореологические расстройства, которые возникают первоначально у больных геморрагическим панкреонекрозом как результат некротических изменений поджелудочной железы, с определенного, довольно раннего этапа сами становятся фактором патогенеза данного заболевания. Доминирующим фактором прогрессивного увеличения вязкости крови у больных панкреонекрозом является нарушение морфофункциональных и физико-химических свой ств эритроцитов на фоне высокой активности протеолитических ферментов, биологически активных аминов и крайней степени токсемии. Background. The pathogenesis of acute pancreatitis and pancreonecrosis is still the focus of researchers and clinicians. The role of hemorheological disorders in these diseases remain uncertain until now. Objectives: to define the role of hemorheological disorders in the pathogenesis of hemorrhagic pancreonecrosis and to study the specifics of the mechanism of these disorders. Patients/Methods. This study included 29 patients (12 women and 17 men, age of 23 to 60 years old) with hemorrhagic pancreatic necrosis. We examined blood viscosity, hematocrit and some erythrocyte properties as count, diameter, aggregation, electrophoretic mobility, deformability and mechanical resistance; other investigated parameters were plasma protein composition, plasma and erythrocytes sialic acid concentrations, lipids, total calcium and fibrinogen concentrations, blood fibrinolytic activity, platelets aggregation activity; total hemocoagulation activity was studied with thromboelastography. Control group contained 15 practically healthy individuals (7 women and 8 men). Results. Expressed disturbances of blood rheological properties, mostly in erythrocytes were detected in patients with pancreonecrosis. Red blood cells (RBC) showed 2-times decreasing of mechanical resistance, of their volume by 18.7%, of deformability by 43.8%, of count by 8.75%. Hematocrit remained normal level due to RBC increased volume (sphericity). RBC aggregation had been increased by 1.8 times. Blood viscosity at the shear rate of 1 s–1 was increased by 3.3 times and at the shear rate of 150 s–1 by 1.58 times. Raised erythrocyte aggregation was caused by a decrease of RBC electrophoretic mobility of 35.9%. Sialic acid concentration in RBC membranes was lower of 20.8% whereas conjugated sialic acid in plasma showed increasing by 2.25 times. Conclusions. RBC morphofunctional and physicochemical disturbances cause the increase in blood viscosity in patients with pancreonecrosis. It is distinguishing feature of hemorheological disorders in hemorrhagic pancreonecrosis developing, seems, due to high activity of proteolytic enzymes and biologically active amines. Of particular importance in hemorrhagic pancreonecrosis belong to platelet involving into intravascular coagulation.

Role of Lysophospholipid Growth Factors in the Modulation of Aqueous Humor Outflow Facility

2004 ◽  
Vol 45 (7) ◽  
pp. 2263 ◽  
Author(s):  
Priyatham Sai Mettu ◽  
Pei-Feng Deng ◽  
Uma K. Misra ◽  
Govind Gawdi ◽  
David L. Epstein ◽  
...  
Keyword(s):  
Growth Factors ◽  
Aqueous Humor ◽  
Outflow Facility ◽  
Aqueous Humor Outflow

The role of protein kinase C in modulation of aqueous humor outflow facility

2003 ◽  
Vol 76 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Rahul N Khurana ◽  
Pei-Feng Deng ◽  
David L Epstein ◽  
P Vasantha Rao
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Aqueous Humor ◽  
Outflow Facility ◽  
Aqueous Humor Outflow ◽  
Kinase C

scholarly journals Progressive spectacle correction in patients with ametropia and primary open-angle glaucoma

The Eye ◽  
2019 ◽  
Vol 125 (2019-1) ◽  
pp. 6-12
Author(s):  
Irina Gndoyan ◽  
Natalya Kuznetsova ◽  
Alexander Derevyanchenko
Keyword(s):  
Intraocular Pressure ◽  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Main Group ◽  
Control Group ◽  
Open Angle ◽  
Aqueous Humor Outflow ◽  
Vision Correction ◽  
Spectacle Correction

Purpose: To determine the role that progressive spectacle lenses play in intraocular pressure compensation and stabilization of the glaucomatous process in patients with primary open-angle glaucoma (POAG) combined with presbyopia and refractive errors. Material and methods. 29 patients (53 eyes) aged 43 to 67 years with I-II stage POAG and a certain type of refractive error were enrolled into study. All patients had intraocular pressure (IOP) compensated to the target level on medication. Patients of the main group (17 people, 32 eyes) used universal progressive spectacle lenses with optimized surface. The patients of the control group (12 people, 21 eyes) used separate monofocal glasses for near and distance vision as a method of ametropia correction. Visual acuity test, refractometry, pneumotonometry, tonography, automated static perimetry were applied for monitoring the patients. The measurements were taken before spectacle correction and a year after its prescription. Results. A decrease of IOP (p=0.01) and an increase of the aqueous humor outflow coefficient (p<0.01) were determined after one year use of the recommended type of vision correction in the main group, whereas in the control group there was an increased level of IOP (p<0.05) and a reduced aqueous humor outflow coefficient (p<0.2). Conclusion. The use of progressive spectacle lenses as a method of permanent vision correction reliably reduces IOP and improves the aqueous humor outflow in patients with early-stage POAG. The absence of negative visual field dynamics indicates the stabilization of the glaucomatous process.

Non-Pathogenic Anti-Heparin/PF4 Antibodies Generated by Low Molecular Weight Heparins Mediate Procoagulant Effects.

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 1091-1091
Author(s):  
Jeanine M. Walenga ◽  
Debra Hoppensteadt ◽  
Evangelos Litinas ◽  
Harry L. Messmore ◽  
Bruce E Lewis ◽  
...  
Keyword(s):  
Thrombin Generation ◽  
Conflicts Of Interest ◽  
Biologically Active ◽  
Control Group ◽  
Heparin Group ◽  
Thrombin Generation Assay ◽  
Enoxaparin Group ◽  
Pre Treatment ◽  
Inhibition Of Thrombin

Abstract Abstract 1091 Introduction: While the incidence of symptomatic heparin-induced thrombocytopenia (HIT) is relatively low with the use of low molecular weights heparins (LMWHs), these agents do generate anti-heparin/PF4 antibodies in 10–20% of treated patients. Dosage, duration, and the pathologic predisposition of the patient influence the quantitative and qualitative nature of these antibodies. It has been suggested that these non-pathogenic antibodies (NPAs) which do not produce symptomatic HIT may, nevertheless, be biologically active and mediate thrombogenic responses. The overall pathophysiologic role of NPAs is unknown at this time. Hypothesis: NPAs generated by LMWHs cause coagulation activation and compromise the anticoagulant effects of the administered LMWH. Study Design: Blood plasma samples collected at baseline and day 10 from patients enrolled in orthopedic surgery clinical trials of LMWHs for the prophylactic management of deep vein thrombosis (Lovenox enoxaparin, sanofi-aventis, n=352; Clivarin reviparin, Abbott, n=380) were retrospectively screened for the presence of anti-heparin/PF4 antibodies using the GTI ELISA method (Waukesha, WI). Positive samples were tested by the 14C-SRA to determine if the antibodies were capable of functionally activating platelets. Both ELISA positive and negative samples were evaluated in an assay of thrombin generation (Technothrombin TGA kit, diaPharma, West Chester, OH). Result: In the enoxaparin study, the baseline pre-treatment samples only showed one patient in the heparin control group to be positive by ELISA. On day 10, 11 of 175 (6.3%) enoxaparin patients had a positive ELISA response, whereas 22 of 177 (12.4%) heparin patients were ELISA positive. None of the samples were 14C-SRA positive. In the thrombin generation assay, the ELISA positive samples showed a lesser inhibition of thrombin generation for both the enoxaparin and heparin groups (270 ± 27 nM TGA enoxaparin group; 220 ± 21 nM TGA heparin group) compared to the thrombin generation response of the ELISA negative samples (190 ± 18 nM TGA enoxaparin group; 160 ± 20 nM TGA heparin group). In the reviparin study, none of the patients were ELISA positive at baseline. On day 10, in the reviparin group 19 of 200 (9.5%) patients were ELISA positive, whereas 28 of 180 (15.6%) heparin control patients had a positive ELISA titer. None of the samples were 14C-SRA positive. In comparison to the baseline (pre-treatment), both the reviparin and heparin treated patients showed an inhibition of thrombin generation (410 ± 27 nM TGA baseline vs 180–290 nM with treatment). However, consistent with the above study, those samples that were ELISA antibody positive showed a lesser inhibition of thrombin generation (240 ± 21 nM TGA reviparin group; 210 ± 16 nM TGA heparin group) in comparison to the ELISA negative samples (190 ± 12 nM TGA reviparin group; 180 ± 14 nM TGA heparin group). Interestingly, the D-dimer levels were found to be higher in the ELISA positive samples in all groups for both studies (p<0.05). Conclusion: These studies suggest a potential pathologic role of NPAs. The results of the thrombin generation studies strongly suggest that the generation of NPAs may result in a reduction of the antithrombotic potential of both LMWH and heparin in treated patients. While the exact mechanism of this process is not clear, dosage adjustment may be useful in those patients who generate NPAs. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Comparative treatment results of non-penetrating surgeries for treating primary open-angle glaucoma

2012 ◽  
Vol 93 (6) ◽  
pp. 996-999
Author(s):  
S N Bulgar ◽  
R F Akhmetshin ◽  
D E Malinin
Keyword(s):  
Intraocular Pressure ◽  
Aqueous Humor ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Hypotensive Effect ◽  
Deep Sclerectomy ◽  
Open Angle ◽  
Flow Reduction ◽  
Aqueous Humor Flow ◽  
Hypotensive Drugs

Aim. To assess the effectiveness of combined non-penetrating surgery: non-penetrating deep sclerectomy and ab externo trabeculectomy in patients with primary open-angle glaucoma depending on type of aqueous humor retention. Methods. Results of surgeries on 72 patients (83 eyes) with primary open-angle glaucoma were analyzed. Patients were divided into 2 groups. Standard ophthalmic examination was added by fluorescein lymphography of the anterior eye to determine the surgery type as well as for hypotensive effect long-term prognosis. Non-penetrating deep sclerectomy and ab externo trabeculectomy were conducted in the first group (67 eyes). In the control group (16 eyes) standard non-penetrating deep sclerectomy was performed. A cytostatic was used as a subconjunctival injections in the post-surgical period. Results. Intraocular pressure was fully compensated in 52 eyes out of 67 (77.6%) in the long-term period after non-penetrating deep sclerectomy and ab externo trabeculectomy in the first group. In 15 eyes (22.4%) topical hypotensive drugs were required for complete compensation of intraocular pressure. Complete compensation of intraocular pressure was registered in patients with moderate aqueous humor flow reduction (trabecular and mixed with prevalence of trabecular forms). Topical hypotensive drugs were required in severe aqueous humor flow reduction (mixed form with prevalence of intrascleral). In the second group, compensation of intraocular pressure was achieved only in early post-surgical period in 12 eyes (75%) with further decrease of hypotensive effect after 4-6 months. There was no complete compensation of intraocular pressure at late post-surgical period. To normalize the intraocular pressure, topical hypotensive drugs were required in 15 eyes (93.8%), surgery was repeated in 1 (6.2%) case. Conclusion. Combined surgery (non-penetrating deep sclerectomy and ab externo trabeculectomy) is more effective compared to non-penetrating deep sclerectomy and is indicated in patients with open-angle glaucoma and moderate aqueous humor flow reduction.

scholarly journals Application of aqueous humor polymerase chain reaction for virus in Posner-Schlossman syndrome

2020 ◽  
Author(s):  
Shuya Tao ◽  
Qin Jiang ◽  
Li Tang ◽  
Xiyan Ding ◽  
Xiumiao Li ◽  
...  
Keyword(s):  
Anterior Chamber ◽  
Aqueous Humor ◽  
Antiviral Treatment ◽  
Control Group ◽  
Case Group ◽  
Viral Detection ◽  
Detection Technology ◽  
Significant Difference ◽  
Positive Rate ◽  
The Difference

Abstract Background To study the effectiveness and safety of aqueous humor PCR viral detection in the diagnosis of PSS. Methods 20 patients (case group) with PSS who suffered from recurrences and poor intraocular pressure (IOP) control by use of drugs underwent anterior chamber puncture. The concentration of different viruses in aqueous humor were detected by PCR. 20 patients (control group) with POAG were given the same treatment and we compared the difference of positive rate of the viruses between two groups. Finally, the effectiveness and safety of aqueous humor PCR viral detection in the diagnosis of PSS were observed. Besides, the advantage of PCR viral detection technology in treatment was evaluated. Results No complications and long-term sequelae related to anterior chamber puncture were found. The total positive rate of aqueous humor viral detection was 30% (6/20) in case group ,and was found none in control group (0/20) (P = 0.027). Then, we divided the case group into two groups. Six virus positive patients were given local and systemic antiviral treatment for 3 months and then interrupted the treatment. Fourteen patients left were given no antiviral treatment. All PSS patients were observed for 9 months from the first time of treatment. There is significant difference between two groups in recurrence rate (P = 0.013). Conclusions Viral infection is a risk factor of PSS. The detection of virus in aqueous humor by PCR is effective and safe for diagnosis of PSS, and helpful for targeted treatment on virus-positive patients.

scholarly journals THE EFFECT OF N-ACETYLCYSTEINE AND DEXAMETHASONE PARENTERAL ADMINISTRATION ON THE BIOCHEMICAL PROCESSES IN THE AQUEOUS HUMOR OF THE ANTERIOR CHAMBER OF THE EYES IN RABBITS WITH EXPERIMENTAL IMMUNOGENIC UVEITIS

2021 ◽  
Vol 19 (5) ◽  
pp. 519-525
Author(s):  
V. G. Marmysh ◽  
◽  
I. A. Kurstak ◽  
Keyword(s):  
Anterior Chamber ◽  
Aqueous Humor ◽  
Horse Serum ◽  
Pharmacological Action ◽  
Albumin Level ◽  
Parenteral Administration ◽  
Control Group ◽  
Albumin Concentration ◽  
Intramuscular Injections ◽  
The Impact

Objective. To evaluate the impact and compare the efficiency of parenteral administration of N-acetylcysteine (NAC) and dexamethasone when used as monotherapy or combination therapy in the treatment of experimental immunogenic uveitis (EIU) in rabbits, as well as to explore the changes of biochemical parameters in the aqueous humor of the eyes in experimental animals. Material and Methods. An experimental study was performed on 45 rabbits (90 eyes). Of these 5 healthy intact rabbits (10 eyes) served as a control group. Acute immunogenic uveitis was caused in 40 rabbits by injecting normal horse serum subcutaneously (5 ml) and then intravitreally (0.07 ml). The animals with experimental uveitis were divided into 8 groups (5 animals each). The first 4 groups – control-1, experiment-1, control-3, experiment-3 – received daily intramuscular injections of placebo, NAC, dexamethasone or a combination of NAC and dexamethasone respectively for 3 days, and thereafter they were withdrawn from the experiment. The remaining 4 groups – control-2, experiment-2, control-4, experiment-4 received, respectively, daily intramuscular injections of placebo, NAC, dexamethasone, a combination of NAC and dexamethasone for 7 days, and after that they were also withdrawn from the experiment. The drugs in the aforementioned groups were used from the moment of the horse serum intravitreal injection. When withdrawing animals from the experiment, aqueous humor was taken from the anterior chamber of their eyes, followed by the evaluation of protein (albumin) concentration and the number of leukocytes. Results. A significant elevation of albumin and the number of leukocytes in the aqueous humor of the eyes in the rabbits with experimental immunogenic uveitis was noted. NAC effectively reduced the level of albumin and the number of leukocytes in the aqueous humor. Dexamethasone showed more efficacy in reducing the investigated aqueous humor biochemichal parameters than NAC. Nevertheless, a synergism of the pharmacological action of NAC and dexamethasone was detected, since their combination had the greatest potency in reduction of albumin level and the number of leukocytes in the aqueous humor of the eyes in the rabbits with experimental immunogenic uveitis, even though the dosage of dexamethasone in the groups with combined (NAC and dexamethasone) therapy was reduced by 50% (1 mg / kg body weight). Conclusion. Parenteral administration of NAC significantly reduces inflammation in EIU. Combination of NAC and dexamethasone showed synergy of action in reducing the intensity of inflammatory process in rabbits with EIU, which is an objective rationale for including NAC in the complex therapy of uveitis, which in turn will reduce a single or course dose of dexamethasone and lower the risks of side effects caused by glucocorticoids.

scholarly journals Matrix Metalloproteinase and Their Inhibitors Concentration and Activity Variations in Aqueous Humor and Plasma of Glaucoma Patients

2017 ◽  
Vol 68 (8) ◽  
pp. 1829-1833
Author(s):  
Iulia Maria Ciotu ◽  
Alina Constantin ◽  
Liliana Voinea ◽  
Valeriu Atanasiu
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinase ◽  
Aqueous Humor ◽  
Open Angle Glaucoma ◽  
The Body ◽  
Plasma Samples ◽  
Open Angle ◽  
Aqueous Humor Outflow ◽  
Cataract Patients

Matrix metalloproteinases (MMPs) are a group of endopeptidases with the role of reorganizing the extracellular matrix of cells through the body. In aqueous humor MMPs have an important role in turnover regulation by acting on the constant remodeling of the trabecular mesh keeping at a constant level the trabecular resistance for a good aqueous humor outflow and consequently of the intraocular pressure.In this paper, we aimed to assess the levels of MMP-2/TIMP-1 and MMP-9/TIMP-2 ratio in aqueous humor samples and in the plasma of patients with open angle glaucoma and cataract patients that serves as control, and to evaluate the gelatinolytic activity MMP-2 and MMP-9 in aqueous humor samples. Aqueous humor and plasma samples were collected from 30 patients, 14 from open angle glaucoma and 16 from cataract patients. Levels of MMP-2, -9 and TIMP -1 and -2 were determined by zymography and immunoassays, using specific kits. The data obtained suggest the presence of a direct correlation between the levels and activity of MMP influencing the accumulation of abnormal matrix and may have an impact on the pathogenesis of open angle glaucoma.\

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