scholarly journals Создание новых форм редиса и редьки (Raphanus sativus L.) с прогнозируемым комплексом хозяйственно ценных признаков при использовании методологии ускоренной селекции

Author(s):  
A.A. Kochetov ◽  
N.G. Sinyavina

В Агрофизическом научноисследовательском институте (ФГБНУ АФИ) при использовании оригинальной методологии ускоренной селекции получены новые высокопродуктивные формы Raphanus sativus L., предназначенные для выращивания в открытом грунте Ленинградской области (дайкон) и в условиях интенсивной светокультуры (линии редиса и гибриды F1 редиса и дайкона). Цель исследований усовершенствование разработанной авторами методологии ускоренной селекции при создании новых высокопродуктивных форм Raphanus sativus L., адаптированных к конкретным условиям выращивания. При проведении исследований изучено внутривидовое разнообразие корнеплодных культур вида Raphanus sativus L. (более 60 образцов разных экологогеографических групп и происхождения) при выращивании их в интенсивной светокультуре. Растения культивировали под лампами ДНаТ400 и ДНаЗ400 при облучении 70 80 Вт/м2 ФАР. Корнеобитаемой средой служил торф с минеральными добавками, увлажняемый ежедневно раствором 1 н Кнопа при чередовании с водой. Оценена реакция различных сортов лобы и дайкона (35 образцов) на действие длинного дня (18часовой фотопериод) и низких яровизирующих температур, провоцирующих преждевременное цветение. Выделены наиболее резистентные к этим факторам образцы, при гибридизации которых получены трансгрессивные формы дайкона с комплексной устойчивостью к раннему стеблеванию. На их основе создан высокопродуктивный сорт дайкона Петербургский, обладающий устойчивостью к действию длинного дня и пониженных температур, предназначенный для весенних посадок в открытый грунт Ленинградской области. При селекции редиса разработана и реализована стратегия получения высокопродуктивных форм, адаптированных для выращивания в интенсивной светокультуре. Выявлены сорта, способные обеспечивать урожай корнеплодов товарного качества до 3 кг/м2 за 25 суток вегетации, а также сорта редиса источники хозяйственно ценных признаков, реализуемых в этих условиях (максимальная длина/диаметр корнеплода, короткий вегетационный период, устойчивость к стеблеванию, компактная розетка листьев, неопушенный лист и др. Подобраны комбинации скрещивания, в потомстве которых получены гетерозисные и трансгрессивные гибриды, на 160 230 превышающие по массе корнеплодов родительские сорта. На их основе созданы высокопродуктивные скороспелые линии редиса для светокультуры с урожайностью 4,5 5 кг/м2 за 23 25 суток от посева. Показана перспективность работы с гибридным потомством в комбинации скрещивания редис дайкон. Получены гетерозисные гибриды F1 с массой корнеплода 100 150 г (товарная урожайность до 7 кг/м2), формирующиеся за 42 дня от посева.New highly productive forms of Raphanus sativus L. were obtained at the Agrophysical Research Institute (FSBSI ARI) agrophysical Research Institute), using the original methodology of accelerated breeding. They are intended for cultivation in the open ground of the Leningrad Region (daikon) and under conditions of intensive light culture (small radish lines and F1 hybrids of small radish and daikon). The aim of the research is to improve the methodology of accelerated selection developed by the authors for the creation of new highly productive forms of Raphanus sativus L. adapted to specific growing conditions. At first stage, the intraspecific diversity of root crops of the species Raphanus sativus L. (more than 60 cultivars of different groups and origin) was studied in intense light culture. Plants were grown under DNAT400 and DRIKZ400 lamps with irradiation intensity of 70 80 W/m2 of PAR. Root medium was the peat with mineral additives, moisturize daily with a Knop solution by alternating with water. The reactions of different varieties of the East Asian radishes (35 cultivars) on long day conditions (18hour photoperiod) and low spring temperatures were evaluated. The most resistant to these stressors cultivars were identified, and hybridization of them produced transgressive forms of daikon with complex resistance. The revealing of these forms and the subsequent stabilizing selection were performed in the open ground in the Leningrad region at sowing in the first decade of may. As a result of the research, a highly productive variety of daikon Peterburgskyi, adapted to spring sowing in the open ground of the Leningrad region, was obtained. When breeding new forms of radish for light culture, the collection of cultivars of different origin was studied and varieties were identified with a yield of commercial root crops up to 3 kg/m2 for 25 days of vegetation, as well as varieties that are sources of economically valuable traits implemented in conditions of intensive light culture (the maximum length/diameter of the root crop, early maturity, resistance to bolting, compact rosette, glabrous leaf type and etc). Combinations of crosses were selected, in the progeny of which heterotic and transgressive hybrids are obtained, that are 160 230 higher than the parent varieties by weight of root crops. On their basis, highly productive early ripening lines of radish for light culture were created with a yield of 4.5 5 kg/m2 for 23 25 days from sowing. Moreover, the prospectivity of selection work with hybrid offspring in a crossbreeding combination of small radish and daikon in light culture is shown. Heterotic F1 hybrids with a root mass of 100 150 g (yield up to 7 kg/m2) formed 42 days after sowing, were obtained.

2013 ◽  
Vol 21 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Piotr Kamiński

ABSTRACT The aim of this study was to obtain new broccoli lines with cytoplasmic male sterility trait for the development of the modern F1 hybrids. CDT70 cauliflower line obtained in the Research Institute of Horticulture, formerly Research Institute of Vegetable Crops, Skierniewice, Poland, with the reliable cytoplasmic male sterility from Raphanus sativus (Ogu-INRA) was selected as a source of this trait. Three broccoli lines: BMi, BCr1 and BCr2 were used as donors of commercial characters in all cross combinations with sterile components. Selected fertile broccoli genotypes were characterized by good quality, uniformity and high level of self-compatibility. The breeding procedure included three consecutive back crosses of male sterile genotypes with fertile broccoli lines that lasted from 2008 to 2012. In each generation, self-compatibility level, the stability of the male sterility trait and ability for the generative propagation of back-crossed genotypes were tested in comparison with donor broccoli lines in the greenhouse. The agronomical and morphological characters of the back-crossed progeny were also evaluated in the field. As a result, three CMS broccoli lines of Bc3 generation with good quality and high seeding index, suitable for the breeding purposes, were obtained.


2020 ◽  
Vol 24 (6) ◽  
pp. 613-624
Author(s):  
A. B. Kurina ◽  
I. A. Kosareva ◽  
A. M. Artemyeva

Radish and small radish (Raphanus sativus L.) are popular and widely cultivated root vegetables in the world, which occupy an important place in human nutrition. Edaphic stressors have a significant impact on their productivity and quality. The main factor determining the phytotoxicity of acidic soils is the increased concentration of mobile aluminum ions in the soil solution. The accumulation of aluminum in root tissues disrupts the processes of cell division, initiation and growth of the lateral roots, the supply of plants with minerals and water. The study of intraspecific variation in aluminum resistance of R. sativus is an important stage for the breeding of these crops. The purpose of this work was to study the genetic diversity of R. sativus crops including 109 accessions of small radish and radish of various ecological and geographical origin, belonging to 23 types, 14 varieties of European, Chinese and Japanese subspecies on aluminum tolerance. In the absence of a rapid assessment methodology specialized for the species studied, a method is used to assess the aluminum resistance of cereals using an eriochrome cyanine R dye, which is based on the recovery or absence of restoration of mitotic activity of the seedlings roots subjected to shock exposure to aluminum. The effect of various concentrations on the vital activity of plants was revealed: a 66-mM concentration of AlCl3 · 6Н2О had a weak toxic effect on R. sativus accessions slowing down root growth; 83 mM contributed to a large differentiation of the small radish accessions and to a lesser extent for radish; 99 mM inhibited further root growth in 13.0 % of small radish accessions and in 7.3 % of radish and had a highly damaging effect. AlCl3 · 6Н2О at a concentration of 99 mM allowed us to identify the most tolerant small radish and radish accessions that originate from countries with a wide distribution of acidic soils. In a result, it was possible to determine the intraspecific variability of small radish and radish plants in the early stages of vegetation and to identify genotypes that are contrasting in their resistance to aluminum. We recommend the AlCl3 · 6Н2О concentration of 83 mM for screening the aluminum resistance of small radish and 99 mM for radish. The modified method that we developed is proposed as a rapid diagnosis of aluminum tolerance for the screening of a wide range of R. sativus genotypes and a subsequent study of contrasting forms during a longer cultivation of plants in hydroponic culture (including elemental analysis of roots and shoots, contrasting in resistance of accessions) as well as reactions of plants in soil conditions.


Author(s):  
A.B. Kurina ◽  
A.M. Artemyeva ◽  
N.G. Sinyavina ◽  
A.A. Kochetov ◽  
G.G. Panova

Описаны биологические особенности редиса (Raphanus sativus L.) коллекции ВИР в условиях интенсивной светокультуры. Результаты свидетельствуют о высоком разнообразии сортовой реакции редиса на выращивание в ИС. Определена амплитуда изменчивости основных морфологических, фенологических признаков и продуктивности в зависимости от сортотипа. Выделены источники хозяйственно ценных признаков для селекции.The present study describes biological features of small radish (Raphanus sativus L.) grown under the intense light culture conditions. Results, obtained using regulated system, demonstrated high variability of reaction to intense light conditions among the varieties of small radish. Amplitude of variability of the main morphological, phenological traits and productivity depending on variety type, was assessed. Samples of small radish, which can be potential sources of economic-valuable traits for future breeding (such as resistance to bolting under the intense light culture, compact rosette, high productivity and marketability) were identified.


2020 ◽  
Author(s):  
A.B. KURINA ◽  

The catalogue contains characteristics of morphological, biological and economically important traits of 118 radish accessions from the VIR collection, grown in two periods, in spring and summer, in open ground. The range of economically valuable traits variability was determined. The catalogue aims to help breeders and scientists to familiarize themselves with the genetic diversity of the VIR radish collection.


2021 ◽  
pp. 76-83
Author(s):  
T. I. Mokryanskaya ◽  
V. F. Gorokhovsckyi

Relevance. One of the most important stages in the selection of agricultural crops for heterosis is the determination of the combinational ability, in particular, the evaluation of the SCS constants of lines of promising hybrid combinations. Purpose: on the basis of the best hybrid combinations, create promising cucumber hybrids for open and protected ground, for various purposes, characterized by a complex of economically useful features, including high fruit yield. Objective: to evaluate the effects of true, hypothetical, competitive heterosis of hybrids and SCA line constants in the studied F1 hybrid combinations.Materials and methods.The research work was carried out in the Pridnestrovian Research Institute of Agriculture in 2014-2018 in greenhouses (spring-summer and summer rotations) and in open ground when growing in a spread and on a trellis. Much attention was paid to the degree and nature of the manifestation of heterosis in F1 hybrids, which were evaluated by early and total yield, the yield of standard fruits, the yield of gherkins and greens. The degree of manifestation of true, hypothetical and competitive heterosis of hybrids is established and the SCA constants in the studied F1 combinations are evaluated. The standards were the F1 Zubrenok hybrids of selection the Pridnestrovian Research Institute of Agriculture and Ajax of the Dutch selection. As testers, JL-6 and L.96 were used. In the studies, selection methods were used-pair and return crosses – backcrosses), self-pollination (incucht), selection (individual, group and mass).Results.With positive effects, all three types of heterosis (true, hypothetical and competitive) were observed in the spring-summer and summer rotations of film greenhouses and open ground when grown in a spread and on a trellis for most economically valuable traits in hybrid combinations 43x57 (seven traits); 71/55x41/86; 65x52 (six); 65x41/86, 65x96, 95x68 (five). High SCA constants were observed in film greenhouses (spring-summer and summer rotations) and in open ground (in the spread and on the trellis) lines in combinations 71/55x41/86 (early, general and standard fruit yield; gherkins yield fractions of 5.1-7.0 and 7.1-9.0 cm (+1.0; +0.9; +10.6; +1.2), (+1.8; +4.9; +39.8; +2.6), (+2.0; +4.4; +36.8; +2.6), (+1.1; +1.5; +12.9; +2.3); 65x52 (gherkins yield fractions of 5.1-7.0 cm) (+12.2; +7.2; +12.5; +7.0); 43x57 (yield of gherkins of the fraction 5.1-7.0 cm) (+1.0; +2.0; +6.7; +2.0).


2015 ◽  
pp. 8-12 ◽  
Author(s):  
G. F. Monakhos ◽  
◽  
A. A. Mironov ◽  
S. M. Tiukhanova ◽  
◽  
...  

Author(s):  
O.P. Ovchinnikova

The aim of the research was to reveal the adaptive features of the original collection material of radish Raphanus sativus L. in terms of "total root yield" and "vitamin C content". Methods. Field, biometric, statistical. The following indicators were used to assess the parameters of adaptive capacity and ecological plasticity: general and specific adaptive capacity of the genotype (GACi and SACi); relative stability (Sgi); coefficient of ecological plasticity (bi); breeding value of the genotype (BVGi). Results. According to the results of research in 2015–2017 for the agro-climatic zone of the Left Bank Forest-Steppe of Ukraine promising varieties as valuable genetic sources for adaptive selection of radish Raphanus sativus L. Among the analyzed varieties were selected 2 samples Lʹodyana burulʹka and Bila tsylindrychna indicators of adaptive potential and higher indicators of «total yield» (Xmed = 21,03–22,10 t/ha, SCG = 12,93–19,31) and vitamin C content in roots (Xmed = 22,47–25,43 mg/100 g, SCGi = 18,26–20,20). Another variety Ilka had a high adaptive potential and the level of manifestation in terms of «total root yield» (Xmed = 22,10 t/ha, SCGi = 11,83). There were 2 samples of 18 dney and Lʹodyana burulʹka, which showed a low dependence on environmental conditions (bi<1) and are valuable genetic sources for adaptive selection. According to the indicator "vitamin C content" in root crops, 5 samples were isolated that had better adaptive characteristics than the standard ruby Rubin: Duet (Xmed = 26,20 mg/100 g, SCGi = 20,76), Bohynya (Xmed = 22,77 mg/100 g, SCGi = 20,63), Snyezhka (Xmed = 23,29 mg/100 g, SCGi = 18,84), Bila (Xmed = 23,30 mg/100 g, SCGi = 18,57) ), Frenchpop (Xmed = 26,27 mg/100 g, SCGi = 20,85). Stable sources on the basis of "vitamin C content" in roots (bi<1) were samples: Duet), Bohynya, Bila, Bila tsylindrychna. Conclusions. There are two high-yielding varieties of sowing radish Lʹodyana burulʹka and Bila tsylindrychna, which in terms of the manifestation of the signs of "total root yield" and "vitamin C content in roots" exceeded the standard standard Ruby. in terms of adaptive potential. Key words: radish, yield, genotype, biochemical composition, adaptive ability.


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