Molecular characterization and expression analysis of hemoglobin cDNA from small radish (Raphanus sativus L var.sativus)

2003 ◽  
Vol 46 (3) ◽  
pp. 161-166 ◽  
Author(s):  
Hye Jin Kim ◽  
Chung Sun An
Keyword(s):  
Molecular Characterization ◽  
Expression Analysis ◽  
Raphanus Sativus ◽  
Raphanus Sativus L ◽  
Small Radish

scholarly journals Molecular Characterization and Expression Profiles of Myrosinase Gene (RsMyr2) in Radish (Raphanus sativus L.)

2014 ◽  
Vol 13 (9) ◽  
pp. 1877-1888 ◽  
Author(s):  
Yan PAN ◽  
Yuan-yuan XU ◽  
Xian-wen ZHU ◽  
Zhe LIU ◽  
Yi-qin GONG ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Raphanus Sativus ◽  
Expression Profiles ◽  
Raphanus Sativus L

scholarly journals Создание новых форм редиса и редьки (Raphanus sativus L.) с прогнозируемым комплексом хозяйственно ценных признаков при использовании методологии ускоренной селекции

2019 ◽  
Author(s):  
A.A. Kochetov ◽  
N.G. Sinyavina
Keyword(s):  
Raphanus Sativus ◽  
Intraspecific Diversity ◽  
F1 Hybrids ◽  
Leningrad Region ◽  
Early Maturity ◽  
Root Crops ◽  
Raphanus Sativus L ◽  
Open Ground ◽  
Small Radish ◽  
Research Institute

В Агрофизическом научноисследовательском институте (ФГБНУ АФИ) при использовании оригинальной методологии ускоренной селекции получены новые высокопродуктивные формы Raphanus sativus L., предназначенные для выращивания в открытом грунте Ленинградской области (дайкон) и в условиях интенсивной светокультуры (линии редиса и гибриды F1 редиса и дайкона). Цель исследований усовершенствование разработанной авторами методологии ускоренной селекции при создании новых высокопродуктивных форм Raphanus sativus L., адаптированных к конкретным условиям выращивания. При проведении исследований изучено внутривидовое разнообразие корнеплодных культур вида Raphanus sativus L. (более 60 образцов разных экологогеографических групп и происхождения) при выращивании их в интенсивной светокультуре. Растения культивировали под лампами ДНаТ400 и ДНаЗ400 при облучении 70 80 Вт/м2 ФАР. Корнеобитаемой средой служил торф с минеральными добавками, увлажняемый ежедневно раствором 1 н Кнопа при чередовании с водой. Оценена реакция различных сортов лобы и дайкона (35 образцов) на действие длинного дня (18часовой фотопериод) и низких яровизирующих температур, провоцирующих преждевременное цветение. Выделены наиболее резистентные к этим факторам образцы, при гибридизации которых получены трансгрессивные формы дайкона с комплексной устойчивостью к раннему стеблеванию. На их основе создан высокопродуктивный сорт дайкона Петербургский, обладающий устойчивостью к действию длинного дня и пониженных температур, предназначенный для весенних посадок в открытый грунт Ленинградской области. При селекции редиса разработана и реализована стратегия получения высокопродуктивных форм, адаптированных для выращивания в интенсивной светокультуре. Выявлены сорта, способные обеспечивать урожай корнеплодов товарного качества до 3 кг/м2 за 25 суток вегетации, а также сорта редиса источники хозяйственно ценных признаков, реализуемых в этих условиях (максимальная длина/диаметр корнеплода, короткий вегетационный период, устойчивость к стеблеванию, компактная розетка листьев, неопушенный лист и др. Подобраны комбинации скрещивания, в потомстве которых получены гетерозисные и трансгрессивные гибриды, на 160 230 превышающие по массе корнеплодов родительские сорта. На их основе созданы высокопродуктивные скороспелые линии редиса для светокультуры с урожайностью 4,5 5 кг/м2 за 23 25 суток от посева. Показана перспективность работы с гибридным потомством в комбинации скрещивания редис дайкон. Получены гетерозисные гибриды F1 с массой корнеплода 100 150 г (товарная урожайность до 7 кг/м2), формирующиеся за 42 дня от посева.New highly productive forms of Raphanus sativus L. were obtained at the Agrophysical Research Institute (FSBSI ARI) agrophysical Research Institute), using the original methodology of accelerated breeding. They are intended for cultivation in the open ground of the Leningrad Region (daikon) and under conditions of intensive light culture (small radish lines and F1 hybrids of small radish and daikon). The aim of the research is to improve the methodology of accelerated selection developed by the authors for the creation of new highly productive forms of Raphanus sativus L. adapted to specific growing conditions. At first stage, the intraspecific diversity of root crops of the species Raphanus sativus L. (more than 60 cultivars of different groups and origin) was studied in intense light culture. Plants were grown under DNAT400 and DRIKZ400 lamps with irradiation intensity of 70 80 W/m2 of PAR. Root medium was the peat with mineral additives, moisturize daily with a Knop solution by alternating with water. The reactions of different varieties of the East Asian radishes (35 cultivars) on long day conditions (18hour photoperiod) and low spring temperatures were evaluated. The most resistant to these stressors cultivars were identified, and hybridization of them produced transgressive forms of daikon with complex resistance. The revealing of these forms and the subsequent stabilizing selection were performed in the open ground in the Leningrad region at sowing in the first decade of may. As a result of the research, a highly productive variety of daikon Peterburgskyi, adapted to spring sowing in the open ground of the Leningrad region, was obtained. When breeding new forms of radish for light culture, the collection of cultivars of different origin was studied and varieties were identified with a yield of commercial root crops up to 3 kg/m2 for 25 days of vegetation, as well as varieties that are sources of economically valuable traits implemented in conditions of intensive light culture (the maximum length/diameter of the root crop, early maturity, resistance to bolting, compact rosette, glabrous leaf type and etc). Combinations of crosses were selected, in the progeny of which heterotic and transgressive hybrids are obtained, that are 160 230 higher than the parent varieties by weight of root crops. On their basis, highly productive early ripening lines of radish for light culture were created with a yield of 4.5 5 kg/m2 for 23 25 days from sowing. Moreover, the prospectivity of selection work with hybrid offspring in a crossbreeding combination of small radish and daikon in light culture is shown. Heterotic F1 hybrids with a root mass of 100 150 g (yield up to 7 kg/m2) formed 42 days after sowing, were obtained.

scholarly journals Genetic diversity of VIR Raphanus sativus L. collections on aluminum tolerance

2020 ◽  
Vol 24 (6) ◽  
pp. 613-624
Author(s):  
A. B. Kurina ◽  
I. A. Kosareva ◽  
A. M. Artemyeva
Keyword(s):  
Genetic Diversity ◽  
Root Growth ◽  
Raphanus Sativus ◽  
Aluminum Tolerance ◽  
Soil Conditions ◽  
Acidic Soils ◽  
Aluminum Resistance ◽  
Aluminum Ions ◽  
Raphanus Sativus L ◽  
Small Radish

Radish and small radish (Raphanus sativus L.) are popular and widely cultivated root vegetables in the world, which occupy an important place in human nutrition. Edaphic stressors have a significant impact on their productivity and quality. The main factor determining the phytotoxicity of acidic soils is the increased concentration of mobile aluminum ions in the soil solution. The accumulation of aluminum in root tissues disrupts the processes of cell division, initiation and growth of the lateral roots, the supply of plants with minerals and water. The study of intraspecific variation in aluminum resistance of R. sativus is an important stage for the breeding of these crops. The purpose of this work was to study the genetic diversity of R. sativus crops including 109 accessions of small radish and radish of various ecological and geographical origin, belonging to 23 types, 14 varieties of European, Chinese and Japanese subspecies on aluminum tolerance. In the absence of a rapid assessment methodology specialized for the species studied, a method is used to assess the aluminum resistance of cereals using an eriochrome cyanine R dye, which is based on the recovery or absence of restoration of mitotic activity of the seedlings roots subjected to shock exposure to aluminum. The effect of various concentrations on the vital activity of plants was revealed: a 66-mM concentration of AlCl3 · 6Н2О had a weak toxic effect on R. sativus accessions slowing down root growth; 83 mM contributed to a large differentiation of the small radish accessions and to a lesser extent for radish; 99 mM inhibited further root growth in 13.0 % of small radish accessions and in 7.3 % of radish and had a highly damaging effect. AlCl3 · 6Н2О at a concentration of 99 mM allowed us to identify the most tolerant small radish and radish accessions that originate from countries with a wide distribution of acidic soils. In a result, it was possible to determine the intraspecific variability of small radish and radish plants in the early stages of vegetation and to identify genotypes that are contrasting in their resistance to aluminum. We recommend the AlCl3 · 6Н2О concentration of 83 mM for screening the aluminum resistance of small radish and 99 mM for radish. The modified method that we developed is proposed as a rapid diagnosis of aluminum tolerance for the screening of a wide range of R. sativus genotypes and a subsequent study of contrasting forms during a longer cultivation of plants in hydroponic culture (including elemental analysis of roots and shoots, contrasting in resistance of accessions) as well as reactions of plants in soil conditions.

scholarly journals Isolation and molecular characterization of the FLOWERING LOCUS C gene promoter sequence in radish (Raphanus sativus L.)

2016 ◽  
Vol 15 (4) ◽  
pp. 763-774 ◽  
Author(s):  
Yuan-yuan XU ◽  
Jing WANG ◽  
Shan-shan NIE ◽  
Dan-qiong HUANG ◽  
Yan WANG ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Raphanus Sativus ◽  
Gene Promoter ◽  
Promoter Sequence ◽  
Flowering Locus C ◽  
Raphanus Sativus L ◽  
Flowering Locus

scholarly journals Биологические особенности редиса (Raphanus sativus L.) при выращивании в условиях интенсивной светокультуры

2019 ◽  
Author(s):  
A.B. Kurina ◽  
A.M. Artemyeva ◽  
N.G. Sinyavina ◽  
A.A. Kochetov ◽  
G.G. Panova
Keyword(s):  
Raphanus Sativus ◽  
Culture Conditions ◽  
High Variability ◽  
Light Conditions ◽  
High Productivity ◽  
Biological Features ◽  
Raphanus Sativus L ◽  
Intense Light ◽  
Compact Rosette ◽  
Small Radish

Описаны биологические особенности редиса (Raphanus sativus L.) коллекции ВИР в условиях интенсивной светокультуры. Результаты свидетельствуют о высоком разнообразии сортовой реакции редиса на выращивание в ИС. Определена амплитуда изменчивости основных морфологических, фенологических признаков и продуктивности в зависимости от сортотипа. Выделены источники хозяйственно ценных признаков для селекции.The present study describes biological features of small radish (Raphanus sativus L.) grown under the intense light culture conditions. Results, obtained using regulated system, demonstrated high variability of reaction to intense light conditions among the varieties of small radish. Amplitude of variability of the main morphological, phenological traits and productivity depending on variety type, was assessed. Samples of small radish, which can be potential sources of economic-valuable traits for future breeding (such as resistance to bolting under the intense light culture, compact rosette, high productivity and marketability) were identified.

scholarly journals Molecular characterization of Turnip mosaic potyvirus (TuMV)-infecting radish (Raphanus sativus L.) crop in India

3 Biotech ◽  
2017 ◽  
Vol 7 (6) ◽  
Author(s):  
Nehanjali Parmar ◽  
Ajay Kumar Thakur ◽  
Pardeep Kumar ◽  
P. D. Thakur ◽  
Sat Vrat Bhardwaj
Keyword(s):  
Molecular Characterization ◽  
Raphanus Sativus ◽  
Raphanus Sativus L
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