Sortase-Modified Cholera Toxoids Show Specific Golgi Localization
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Cholera toxoid is an established tool for use in cellular tracing in neuroscience and cell biology. We use a sortase-labelling approach to generate site-specifically <i>N</i>-terminally modified variants of both the A2-B<sub>5</sub> heterohexamer and B<sub>5</sub> pentamer forms of the toxoid. Both forms of the toxoid are endocytosed by GM1-positive mammalian cells, and while the heterohexameric toxoid was principally localized in the ER, the B<sub>5</sub> pentamer showed an unexpected localization in the <i>medial/trans</i> Golgi. This study suggests a future role for specifically-labelled cholera toxoids in live-cell imaging beyond their current applications in neuronal tracing and labelling of lipid-rafts in fixed cells.
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2015 ◽
Vol 166
(1-4)
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pp. 101-103
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2006 ◽
Vol 174
(4)
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pp. 481-484
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2014 ◽
Vol 55
(suppl 1)
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pp. i129-i130
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