scholarly journals Fast and Quantitative Phospholipidomic Analysis of SH-SY5Y Neuroblastoma Cell Cultures Using LC-MS/MS and 31P NMR

2019 ◽  
Author(s):  
Martin Jakubec ◽  
Espen Bariås ◽  
Fedor Kryuchkov ◽  
Linda Veka Hjørnevik ◽  
Øyvind Halskau
Keyword(s):  
Plasma Membrane ◽  
Fatty Acid ◽  
Cell Cultures ◽  
Membrane Fraction ◽  
31P Nmr ◽  
Neuroblastoma Cell ◽  
Elution Profile ◽  
Extraction Techniques ◽  
Lipid Species ◽  
Lipid Separation

Phospholipid quantification by <sup>31</sup>P NMR and lipid separation and detection by LC-MS/MS. Fatty acid quantification using scripted reconstruction of elution profile for each fatty acid associated with each lipid species. The combination of NMR and and MS data is complementary and allows better quantification and more complete description than each approach would allow by itself. Datasets exists for both whole-cell and plasma membrane fraction, and we also describe and document the extraction techniques.

scholarly journals Fast and Quantitative Phospholipidomic Analysis of SH-SY5Y Neuroblastoma Cell Cultures Using LC-MS/MS and 31P NMR

2019 ◽  
Author(s):  
Martin Jakubec ◽  
Espen Bariås ◽  
Fedor Kryuchkov ◽  
Linda Veka Hjørnevik ◽  
Øyvind Halskau
Keyword(s):  
Plasma Membrane ◽  
Fatty Acid ◽  
Cell Cultures ◽  
Membrane Fraction ◽  
31P Nmr ◽  
Neuroblastoma Cell ◽  
Elution Profile ◽  
Extraction Techniques ◽  
Lipid Species ◽  
Lipid Separation

Phospholipid quantification by <sup>31</sup>P NMR and lipid separation and detection by LC-MS/MS. Fatty acid quantification using scripted reconstruction of elution profile for each fatty acid associated with each lipid species. The combination of NMR and and MS data is complementary and allows better quantification and more complete description than each approach would allow by itself. Datasets exists for both whole-cell and plasma membrane fraction, and we also describe and document the extraction techniques.

scholarly journals Fast and Quantitative Phospholipidomic Analysis of SH-SY5Y Neuroblastoma Cell Cultures Using LC-MS/MS and 31P NMR

2019 ◽  
Author(s):  
Martin Jakubec ◽  
Espen Bariås ◽  
Fedor Kryuchkov ◽  
Linda Veka Hjørnevik ◽  
Øyvind Halskau
Keyword(s):  
Plasma Membrane ◽  
Fatty Acid ◽  
Cell Cultures ◽  
Membrane Fraction ◽  
31P Nmr ◽  
Neuroblastoma Cell ◽  
Elution Profile ◽  
Extraction Techniques ◽  
Lipid Species ◽  
Lipid Separation

Phospholipid quantification by <sup>31</sup>P NMR and lipid separation and detection by LC-MS/MS. Fatty acid quantification using scripted reconstruction of elution profile for each fatty acid associated with each lipid species. The combination of NMR and and MS data is complementary and allows better quantification and more complete description than each approach would allow by itself. Datasets exists for both whole-cell and plasma membrane fraction, and we also describe and document the extraction techniques.

scholarly journals The Ca2+-activated polyphosphoinositide phosphodiesterase of human and rabbit neutrophil membranes

1984 ◽  
Vol 221 (2) ◽  
pp. 477-482 ◽  
Author(s):  
S Cockcroft ◽  
J M Baldwin ◽  
D Allan
Keyword(s):  
Fatty Acid Composition ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Phospholipase C ◽  
Acid Composition ◽  
Membrane Fraction ◽  
Plasma Membranes ◽  
Degradation Products ◽  
Diacylglycerol Kinase ◽  
Plasma Membrane Fraction

Addition of Ca2+ to a plasma-membrane fraction derived from human or rabbit neutrophils led to the specific breakdown of polyphosphoinositides. The degradation products were identified as diacylglycerol and inositol bis- and tris-phosphate, thus demonstrating the presence of a Ca2+-activated phospholipase C. The newly generated diacylglycerol resembled the polyphosphoinositides in its fatty acid composition, and in the presence of MgATP2- it was converted into phosphatidate. These results therefore demonstrate the presence in neutrophil plasma membranes not only of polyphosphoinositide phosphodiesterase but also of diacylglycerol kinase.

scholarly journals Heat stress elicits remodeling in the anther lipidome of peanut

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Zolian S. Zoong Lwe ◽  
Ruth Welti ◽  
Daniel Anco ◽  
Salman Naveed ◽  
Sachin Rustgi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Heat Stress ◽  
Fatty Acid ◽  
Membrane Lipids ◽  
Fatty Acid Desaturase ◽  
Susceptible Genotype ◽  
Unsaturated Lipid ◽  
Lipid Species ◽  
Lipid Unsaturation ◽  
Fatty Acid Amount

AbstractUnderstanding the changes in peanut (Arachis hypogaea L.) anther lipidome under heat stress (HT) will aid in understanding the mechanisms of heat tolerance. We profiled the anther lipidome of seven genotypes exposed to ambient temperature (AT) or HT during flowering. Under AT and HT, the lipidome was dominated by phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TAG) species (> 50% of total lipids). Of 89 lipid analytes specified by total acyl carbons:total carbon–carbon double bonds, 36:6, 36:5, and 34:3 PC and 34:3 PE (all contain 18:3 fatty acid and decreased under HT) were the most important lipids that differentiated HT from AT. Heat stress caused decreases in unsaturation indices of membrane lipids, primarily due to decreases in highly-unsaturated lipid species that contained 18:3 fatty acids. In parallel, the expression of Fatty Acid Desaturase 3-2 (FAD3-2; converts 18:2 fatty acids to 18:3) decreased under HT for the heat-tolerant genotype SPT 06-07 but not for the susceptible genotype Bailey. Our results suggested that decreasing lipid unsaturation levels by lowering 18:3 fatty-acid amount through reducing FAD3 expression is likely an acclimation mechanism to heat stress in peanut. Thus, genotypes that are more efficient in doing so will be relatively more tolerant to HT.

scholarly journals Unusual tolerance to high temperatures in a new herbicide-resistant D1 mutant from Glycine max (L.) Merr. cell cultures deficient in fatty acid desaturation

Planta ◽  
2001 ◽  
Vol 212 (4) ◽  
pp. 573-582 ◽  
Author(s):  
Miguel Alfonso ◽  
Inmaculada Yruela ◽  
Susana Almárcegui ◽  
Elena Torrado ◽  
María A. Pérez ◽  
...  
Keyword(s):  
Glycine Max ◽  
Fatty Acid ◽  
High Temperatures ◽  
Cell Cultures ◽  
Fatty Acid Desaturation ◽  
Herbicide Resistant ◽  
Glycine Max L
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