scholarly journals Identification a potential therapeutic target in castrate resistance prostate cancer by using microarray

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Massar Ibrahim Shekhan
Keyword(s):  
Prostate Cancer ◽  
Cell Line ◽  
Lncap Cell ◽  
Early Stage ◽  
Cancer Cell Line ◽  
Mrna Levels ◽  
Line Model ◽  
Cell Line Model ◽  
Number Of Patients ◽  
Significant Difference

The androgen receptor (AR) has a crucial role in the proliferation and progression of prostate cancer. anti-androgen therapy is used in the early stage of the disease and the patients respond well,howevera significant number of patients eventually will develop resistance,entering a castrate-resistant state,with very poor prognosis,which is a major clinical challenge at the moment. Enzalutamide,have shown potential in the treatment of CRPC patients,but response is just 50% and the development of resistance to these are challenging clinic. This study aims to investigate the whole genomic expression of LNCaP prostate cancer cell line model,compared to resistant to Enzalutamide model. TROP-2 demonstrates high expression in Enzalutamide resistant cells in our gene microarray,which mightsuggest TROP-2 may serve as a biomarker of resistance. The preliminary data reveal an increase in expression of TROP-2 in the LNCaP-Enz-R cell line model,compared with parental LNCaP cell,at both the protein and mRNA levels. siRNA againstTROP-2 led a decreases in a proliferation and cell cycle progression of the LNCaP-Enz-R cell line,whereas no significant difference was noticed in the parental,androgen sensitive LNCaP cell line. In addition,Knockdown of TROP-2 decrease migration of LNCaP-Enz-R cell line significantly,while no effect was observed in parental LNCaP cell

Vitamin D-mediated growth inhibition of an androgen-ablated LNCaP cell line model of human prostate cancer

2002 ◽  
Vol 186 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Eddy S Yang ◽  
Carol A Maiorino ◽  
Bernard A Roos ◽  
Selena R Knight ◽  
Kerry L Burnstein
Keyword(s):  
Prostate Cancer ◽  
Vitamin D ◽  
Growth Inhibition ◽  
Cell Line ◽  
Lncap Cell ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Line Model ◽  
Lncap Cell Line ◽  
Cell Line Model

scholarly journals A dock derived compound against laminin receptor (37 LR) exhibits anti-cancer properties in a prostate cancer cell line model

Oncotarget ◽  
2017 ◽  
Vol 9 (5) ◽  
pp. 5958-5978 ◽  
Author(s):  
Charles Samuel Umbaugh ◽  
Adriana Diaz-Quiñones ◽  
Manoel Figueiredo Neto ◽  
Joseph J. Shearer ◽  
Marxa L. Figueiredo
Keyword(s):  
Prostate Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Cancer Cell Line ◽  
Prostate Cancer Cell Line ◽  
Laminin Receptor ◽  
Line Model ◽  
Cell Line Model ◽  
Anti Cancer

scholarly journals Antiproliferative effect of rosehip tea phenolics in prostate cancer cell lines

2020 ◽  
Vol 45 (4) ◽  
pp. 423-428
Author(s):  
Ali Mert Özgönül ◽  
Aycan Aşık ◽  
Burak Durmaz ◽  
Ramin Aslaminabad ◽  
Cumhur Gündüz ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Lncap Cell ◽  
Prostate Cancer Cell ◽  
Cancer Cell Line ◽  
Prostate Cancer Cell Line ◽  
Antiproliferative Effect ◽  
Natural Sources

AbstractObjectivesRecently, phenolic compounds (quercetin, kaempferol, ellagic acid (EA), and myricetin) as natural sources have been suggested to be used for treatment and chemoprevention of prostate cancer. Since rosehip includes the above molecules in high concentration, we set out to investigate possible anti-proliferative effect of rosehip tea on the prostate cancer cell line.MethodsThe flavonol content of rosehip tea prepared at different temperatures and time intervals was determined first and then the antiproliferative effect of tea samples was established by adding tea samples to the prostate cancer cell line (VCaP and LNCaP).ResultsQuercetin was more effective in LNCaP cell than in VCaP cell (IC50 = 20 and 200 μM, respectively). The boiled fruit shredded at minute 7 showed the highest levels of quercetin, EA and kaempferol and the boiled fruit at minute 7 had the highest levels of kaempferol and EA. The tea samples were prepared in concentrations relevant to their IC50 values, added to the VCaP and LNCaP cell lines. The antiproliferative effect of rosehip tea on VCaP cells was slightly greater than that of LNCaP cells.ConclusionEach of the flavonols exhibits an antiproliferative effect. Our data clearly indicated that rosehip as a natural source of all flavonols had an antiproliferative effect on androgen-sensitive prostate cancer. Now that it is important to use natural sources in cancer, rosehip seems to be a promising natural product to be used to treat the prostate illness.

scholarly journals Human-derived alleles in SOST and RUNX2 3′UTRs cause differential regulation in a bone cell-line model

2021 ◽  
Author(s):  
Juan Moriano ◽  
Núria Martínez-Gil ◽  
Alejandro Andirkó ◽  
Susana Balcells ◽  
Daniel Grinberg ◽  
...  
Keyword(s):  
Cell Line ◽  
Homo Sapiens ◽  
Bone Cell ◽  
Genetic Differences ◽  
Luciferase Reporter ◽  
Mineral Density ◽  
Line Model ◽  
Cell Line Model ◽  
Significant Difference ◽  
The Impact

AbstractThe inquiry into the phenotypic features that set apart human species, such as a light, gracile skeleton and a rounded skull characteristic of Homo sapiens, can now benefit from the examination of ancient genomes. These have added a new layer of analysis allowing the identification of genetic differences between species like ours and our closest extinct relatives. Most of these genetic differences are non-coding changes with unknown functional consequences, and dissecting their putative regulatory effect remains challenging. Here we focus on the three prime untranslated regions (3’UTR), known to play a critical role in messenger RNA regulation and a plausible locus for divergent regulation between Homo species. We report a set of genes with derived 3’UTR changes in either the Homo sapiens or the Neanderthal/Denisovan lineages and experimentally evaluate the impact of 3’UTR variants in four genes: E2F6, GLI3, RUNX2 and SOST. We performed a luciferase reporter assay in a bone cell-line model and found a statistically significant difference for the 3’UTR variants of SOST (Homo sapiens-derived) and RUNX2 (Neanderthal/Denisovan-derived). The differential expression caused by these variants in our experimental model points to species differences in bone mineral density. Thus, this study adds insights into the functional effects of regulatory variants that emerged in recent human evolution.

Threshold Effect of Free Radical Quenching in a Progressive Breast Cancer Cell Line Model

2015 ◽  
Vol 4 (1) ◽  
pp. 1-11
Author(s):  
Sriram Sridharan ◽  
Anirudda Datta ◽  
Rola Barhoumi ◽  
Donika Shpati ◽  
Usha Venkatraj ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Free Radical ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Threshold Effect ◽  
Line Model ◽  
Cell Line Model
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