Expression of connexin 32 and connexin 43 in adenomatous polyps and hyperplastic polyps

Background: Aberrant DNA methylation is a common molecular feature in colorectal cancer (CRC). Hypermethylation of miR-200b promoter, as an epigenetic factor, is involved in CRC tumorigenesis. The methylation status of miR-200b has been examined in CRC and adjacent normal tissues. Objectives: This study aimed to investigate miR-200b methylation in a series of colorectal adenomatous polyps, hyperplastic polyps, and adenocarcinoma tissues as precursors of CRC in the Iranian population for the first time. Materials and Methods: In this cross-sectional study (2017-2018), the methylation status of the miR-200b promoter was investigated using methylation-specific PCR in 131 fresh samples, including 30 adenocarcinoma specimens, 17 tumor-adjacent normal tissues, 78 primary lesions (55 adenomatous polyps and 23 hyperplastic polyps) and 6 healthy individuals. Results: Methylation of miR-200b was detected in adenocarcinoma samples (86%) and adenomatous polyps (85%); however, most of the hyperplastic polyps were unmethylated (69.6%). Neither control individuals nor tumor-adjacent normal tissues exhibited methylation in the miR-200b promoter. Aberrant methylation of miR-200b was significantly more common in tumor tissues and adenomatous polyps than in hyperplastic polyps (P<0.0001) and tumor-adjacent normal samples (P<0.0001). Conclusion: Methylation status of the miR-200b promoter was significantly altered during CRC development and may be identified as an attractive biomarker for the early detection of the disease.

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Su1035 Hyperplastic Polyps Predict the Subsequent Occurrence of Adenomatous Polyps on Follow-up Colonoscopy

Gastroenterology ◽

10.1016/s0016-5085(13)61403-1 ◽

2013 ◽

Vol 144 (5) ◽

pp. S-381

Author(s):

Amnon Sonnenberg ◽

Robert M. Genta

Keyword(s):

Adenomatous Polyps ◽

Hyperplastic Polyps

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Gastric polyps: a retrospective analysis of 26,000 digestive endoscopies

Arquivos de Gastroenterologia ◽

10.1590/s0004-28032007000100004 ◽

2007 ◽

Vol 44 (1) ◽

pp. 14-17 ◽

Cited By ~ 66

Author(s):

Drausio Jefferson Morais ◽

Ademar Yamanaka ◽

José Murilo Robilotta Zeitune ◽

Nelson Adami Andreollo

Keyword(s):

Hyperplastic Polyp ◽

Gastric Polyp ◽

Adenomatous Polyps ◽

Fundic Gland ◽

Digestive Endoscopy ◽

Gastric Lesions ◽

Hyperplastic Polyps ◽

Gastric Polyps ◽

Fundic Gland Polyps ◽

Upper Digestive Endoscopy

BACKGROUND: Gastric polyps are small gastric lesions, asymptomatic in most cases and are generally discovered inadvertently during upper digestive endoscopy. AIM: To retrospectively review the characteristics and frequency of gastric polyps, derived from the gastric mucosal epithelium in a large series of endoscopies. METHODS: One hundred and fifty three patients in a series of 26,000 consecutive upper digestive endoscopies done over a 5-year period, being that each patient had only one examination were analyzed and their histological and Yamada classification, as well as their location, size, histopathological findings and treatment studied. All patients had at least one gastric polyp, as confirmed by histological examination. RESULTS: The polyps were classified as hyperplastic, adenomatous and fundic gland polyps. The most of them measure less than 1 cm (hyperplastic polyps - 60,5%; adenomatous polyps - 73,6%; fundic gland polyps - 72%). Hyperplastic polyps were the most frequent and accounted for 71.3% of the cases, whereas fundic gland polyps accounted for 16.3% and adenomatous polyps for 12.4%. Hyperplastic and adenomatous polyps were primarily single, whereas fundic gland polyps tended to be multiple. A carcinoma was detected in one hyperplastic polyp (0.9%) and in two adenomatous polyps (10.5%). High grade dysplastic foci were found in four adenomatous polyps (21%). CONCLUSIONS: The digestive endoscopy is the safest and efficient method for the diagnosis of the gastric polyps, that in most of the patients does not show characteristic symptoms. The histopathological definition is not possible to the endoscopic glance being needed the pathologist's aid, once the conduct to be adopted will depend on the result of the biopsy.

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Expression of connexin 32 and connexin 43 in the cerebral cortex of patients with refractory epilepsy

LaboratoriumsMedizin ◽

10.1515/labmed-2015-0064 ◽

2017 ◽

Vol 41 (1) ◽

pp. 33-40 ◽

Cited By ~ 1

Author(s):

Lichao Sun ◽

Lizhi Zhang ◽

Junqiang Feng ◽

Jiqing Qiu ◽

Weihong Lin

Keyword(s):

Connexin 43 ◽

Brain Injuries ◽

Refractory Epilepsy ◽

Control Group ◽

Connexin 32 ◽

Cx43 Expression ◽

Surgical Treatments ◽

The Brain ◽

Experimental Group ◽

Junction Proteins

AbstractBackground:This study aimed to examine the expression of the gap junction proteins connexin 32 (CX32) and connexin 43 (CX43) in the epileptic foci of the brain in patients with refractory epilepsy, in order to provide insight into the pathogenesis and treatment of refractory epilepsy.Methods:The experimental group consisted of 30 patients with refractory epilepsy who received surgical treatments. The control group consisted of six patients with traumatic brain injuries who underwent emergency surgery. Expression of CX32 and CX43 was assessed by immunohistochemistry and immune electron microscopy of surgically extracted brain epileptic foci and compared with that in brain tissues of the control group.Results:The expression of CX32 and CX43 was significantly higher in the experimental group than that in the control group (p<0.001). Significantly more colloidal gold particles-labeled CX32 and CX43 were observed on the membranes of nerve cells in the experimental group than in the control group.Conclusions:Brain epileptic foci show increased CX32 and CX43 expression, suggesting that gap junctions formed by CX32 and CX43 may contribute to the incidence and progression of epilepsy.

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A Case of Rapidly Progressing Gastric Hyperplastic Polyps to Adenomatous Polyps

The American Journal of Gastroenterology ◽

10.14309/00000434-201010001-00113 ◽

2010 ◽

Vol 105 ◽

pp. S42-S43

Author(s):

Supriya Tigadi ◽

Micheal Tadros ◽

Marcy Coach ◽

John Birk ◽

Joe Anderson

Keyword(s):

Adenomatous Polyps ◽

Hyperplastic Polyps

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Prevalence and Risk Factors of Asymptomatic Colorectal Polyps in Taiwan

Gastroenterology Research and Practice ◽

10.1155/2014/985205 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 9

Author(s):

Fu-Wei Wang ◽

Ping-I Hsu ◽

Hung-Yi Chuang ◽

Ming-Shium Tu ◽

Guang-Yuan Mar ◽

...

Keyword(s):

Risk Factors ◽

Body Mass Index ◽

Alcohol Consumption ◽

Body Mass ◽

High Body Mass Index ◽

Colorectal Polyps ◽

Adenomatous Polyps ◽

Hyperplastic Polyps ◽

Current Smoking ◽

Asymptomatic Subjects

Purpose. To investigate the prevalence and risk factors of hyperplastic and adenomatous colorectal polyps in a Taiwanese general population.Methods. From January 2009 to December 2011, consecutive asymptomatic subjects undergoing a routine health check-up were evaluated by colonoscopy. The colorectal polyps were assessed, and medical history and demographic data were obtained from each patient. Logistic regression analysis was conducted to search the independent risk factors for asymptomatic hyperplastic and adenomatous colorectal polyps.Results. Of the 1899 asymptomatic subjects, the prevalences of hyperplastic polyps and adenomatous polyps were 11.1% and 16.1%, respectively. Multivariate analysis revealed that high body mass index (BMI>25: OR, 1.32, 95% CI, 1.05–1.71) and current smoking (OR, 1.87, 95% CI, 1.42–2.71) were independent predictors for hyperplastic colorectal polyps. Age over 60 years old (OR, 3.49, 95% CI, 1.86–6.51), high body mass index (BMI>25: OR, 1.75, 95% CI, 1.21–2.71), heavy alcohol consumption (OR, 2.01, 95% CI, 1.02–3.99), and current smoking (OR, 1.31, 95% CI, 1.04–1.58) were independent predictors for adenomatous colorectal polyps.Conclusion. High BMI and smoking are common risk factors for both adenomatous and hyperplastic polyps. Old age and alcohol consumption are additional risk factors for the development of adenomatous polyps.

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