Methylation Status of miR-200b Promoter in Colorectal Polyp and Adenocarcinoma Tissues

2021 ◽  
Keyword(s):  
Methylation Status ◽  
Colorectal Polyp ◽  
Cross Sectional Study ◽  
Adenomatous Polyps ◽  
Aberrant Methylation ◽  
Hyperplastic Polyps ◽  
Molecular Feature ◽  
Cross Sectional ◽  
Normal Tissues ◽  
Specific Pcr

Background: Aberrant DNA methylation is a common molecular feature in colorectal cancer (CRC). Hypermethylation of miR-200b promoter, as an epigenetic factor, is involved in CRC tumorigenesis. The methylation status of miR-200b has been examined in CRC and adjacent normal tissues. Objectives: This study aimed to investigate miR-200b methylation in a series of colorectal adenomatous polyps, hyperplastic polyps, and adenocarcinoma tissues as precursors of CRC in the Iranian population for the first time. Materials and Methods: In this cross-sectional study (2017-2018), the methylation status of the miR-200b promoter was investigated using methylation-specific PCR in 131 fresh samples, including 30 adenocarcinoma specimens, 17 tumor-adjacent normal tissues, 78 primary lesions (55 adenomatous polyps and 23 hyperplastic polyps) and 6 healthy individuals. Results: Methylation of miR-200b was detected in adenocarcinoma samples (86%) and adenomatous polyps (85%); however, most of the hyperplastic polyps were unmethylated (69.6%). Neither control individuals nor tumor-adjacent normal tissues exhibited methylation in the miR-200b promoter. Aberrant methylation of miR-200b was significantly more common in tumor tissues and adenomatous polyps than in hyperplastic polyps (P<0.0001) and tumor-adjacent normal samples (P<0.0001). Conclusion: Methylation status of the miR-200b promoter was significantly altered during CRC development and may be identified as an attractive biomarker for the early detection of the disease.

Endoscopic and Histopathological Characteristics of Polypoid and Non-Polypoid Colorectal Lesions in Patients of a Social Security Hospital of Lambayeque, Peru

2021 ◽  
Vol 15 (8) ◽  
pp. 2284-2287
Author(s):  
Jhon Franksis Munoz Chumpen ◽  
Mario J. Valladares-garrido
Keyword(s):  
Social Security ◽  
Transverse Colon ◽  
Cross Sectional Study ◽  
Hyperplastic Polyps ◽  
Sectional Study ◽  
Cross Sectional ◽  
Size And Shape ◽  
Intestinal Polyps ◽  
Paris Classification

Objective: To describe the endoscopic and histopathological characteristics of polypoid and non-polypoid colorectal lesions at the Luis Heysen Inchaustegui hospital, Peru, 2017-2018. Materials and methods: Descriptive-analytical cross-sectional study. We study epidemiological, endoscopic and histopathological variables. location, Size and shape of the lesions taking into account the Paris classification. Results: Endoscopically, of a total of 81 colorectal lesions, the majority were non-polypoid (71.6%). Lesions smaller than 10mm represented 90.1% in non-polypoid lesions and 56.5 % in polypoid lesions. Histopathologically, non-adenomatous lesions predominated (53.0%). Conclusions: The most frequent lesions were non-polypoid, the main location being the rectum; while in polypoid lesions, the transverse colon. Regarding size, lesions smaller than 10mm predominated in both non-polypoid and polypoid lesions. Histopathologically, the most frequent were non-adenomatous lesions (hyperplastic polyps); for its part, the predominant adenoma subtype was tubular. Keywords: Adenomas; Polyps; Intestinal Polyps; Colon (source: DeCS BIREME).

scholarly journals Prevalence and Serotype Determination of Streptococcus agalactiae Isolated From Non-Pregnant Women in Tehran, Iran

2020 ◽  
Author(s):  
Leila Goudarzi ◽  
Mohammad Bagher Khalili ◽  
Mahmood Vakili ◽  
Maryam Sadeh
Keyword(s):  
Pregnant Women ◽  
Streptococcus Agalactiae ◽  
Group B Streptococcus ◽  
Cross Sectional Study ◽  
Chi Square ◽  
Cross Sectional ◽  
Specific Pcr ◽  
Pcr Method ◽  
Group B ◽  
Tehran Area

Consequence of Streptococcus agalactiae, Group B Streptococcus (GBS) relating infant’s diseases are well documented. Although many women carry this bacterium in their vagina, they may transfer to their infant during delivery and may result in different neonatal invasive diseases. The aim of this study was to determine the prevalence of GBS and serotyping the isolated species among un-selective non-pregnant women who attended two gynecology clinics in Tehran. In this cross-sectional study, a total of 560 vaginal samples collected from non-pregnant women. Following inoculation of the specimen on Blood Agar, the standard technology was applied for the final identification of GBS. Detected GBS species were further confirmed using specific PCR directed on dlts gene. Capsular serotyping was done by using the multiplex PCR method. The chi-square method was used for statistical analysis. Fifty (8.9%) out of 560 non-pregnant women were carriers of GBS. The most common types were III (36%), followed by type II (32%), Ia (26%), and Ib (6%), respectively. Results represent that the prevalence rate of GBS in non-pregnant women was reliable and similar to what obtained from pregnant women. In addition, the serotype III was found the most dominant types, as well as other investigations in the Tehran area. Therefore, vaccine designation based on type III is recommended.

scholarly journals AN UPWARD TREND IN DNA P16INK4A METHYLATION PATTERN AND HIGH RISK HPV INFECTION ACCORDING TO THE SEVERITY OF THE CERVICAL LESION

2013 ◽  
Vol 55 (5) ◽  
pp. 329-334 ◽  
Author(s):  
Fernanda Nahoum Carestiato ◽  
Larissa Alves Afonso ◽  
Natalia Moyses ◽  
Gutemberg Leao Almeida Filho ◽  
Luis Guillermo Coca Velarde ◽  
...  
Keyword(s):  
High Risk ◽  
Methylation Status ◽  
Hpv Infection ◽  
Cross Sectional Study ◽  
Methylation Pattern ◽  
Low Grade ◽  
Tobacco Exposure ◽  
Upward Trend ◽  
Cross Sectional ◽  
Risk Of Cancer

SUMMARY High-risk human papillomavirus (hr-HPV) infection is necessary but not sufficient for cervical cancer development. Recently, P16INK4A gene silencing through hypermethylation has been proposed as an important cofactor in cervical carcinogenesis due to its tumor suppressor function. We aimed to investigate P16INK4A methylation status in normal and neoplastic epithelia and evaluate an association with HPV infection and genotype. This cross-sectional study was performed with 141 cervical samples from patients attending Hospital Moncorvo Filho, Rio de Janeiro. HPV detection and genotyping were performed through PCR and P16INK4A methylation by nested-methylation specific PCR (MSP). HPV frequency was 62.4% (88/141). The most common HPV were HPV16 (37%), HPV18 (16.3%) and HPV33/45(15.2%). An upward trend was observed concerning P16INK4A methylation and lesion degree: normal epithelia (10.7%), low grade lesions (22.9%), high grade (57.1%) and carcinoma (93.1%) (p < 0.0001). A multivariate analysis was performed to evaluate an association between methylation, age, tobacco exposure, HPV infection and genotyping. A correlation was found concerning methylation with HPV infection (p < 0.0001), hr-HPV (p = 0.01), HSIL (p < 0.0007) and malignant lesions (p < 0.0001). Since viral infection and epigenetic alterations are related to cervical carcinoma, we suggest that P16INK4A methylation profile maybe thoroughly investigated as a biomarker to identify patients at risk of cancer.

scholarly journals Multiple gene promoter methylation and clinical stage in adjacent normal tissues: Effect on prognosis of colorectal cancer in Taiwan

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chih-Hsiung Hsu ◽  
Cheng-Wen Hsiao ◽  
Chien-An Sun ◽  
Wen-Chih Wu ◽  
Tsan Yang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Tumor Tissue ◽  
Clinical Stage ◽  
Methylation Status ◽  
Aberrant Methylation ◽  
Event Free Survival ◽  
Free Survival ◽  
Normal Tissues ◽  
Colorectal Cancer Patients

AbstractThis study provide an insight that the panel genes methylation status in different clinical stage tended to reflect a different prognosis even in matched normal tissues, to clinical recommendation. We enrolled 153 colorectal cancer patients from a medical center in Taiwan and used the candidate gene approach to select five genes involved in carcinogenesis pathways. We analyzed the relationship between DNA methylation with different cancer stages and the prognostic outcome. There were significant trends of increasing risk of 5-year time to progression and event-free survival of subjects with raising number of hypermethylation genes both in normal tissue and tumor tissue. The group with two or more genes with aberrant methylation in the advanced cancer stages (Me/advanced) had lower 5-year event-free survival among patients with colorectal cancer in either normal or tumor tissue. The adjusted hazard ratios in the group with two or more genes with aberrant methylation with advanced cancer stages (Me/advanced) were 8.04 (95% CI, 2.80–23.1; P for trend <0.01) and 8.01 (95% CI, 1.92–33.4; P for trend <0.01) in normal and tumor tissue, respectively. DNA methylation status was significantly associated with poor prognosis outcome. This finding in the matched normal tissues of colorectal cancer patients could be an alternative source of prognostic markers to assist clinical decision making.

Obesity and colorectal adenomatous polyps: A cross-sectional study in Korean adults

Obesity ◽  
2013 ◽  
Vol 22 (2) ◽  
pp. 518-525 ◽  
Author(s):  
Ji Young Lee ◽  
Sang Mi Kwak ◽  
Seung-Kwon Myung ◽  
Sun Ha Jee
Keyword(s):  
Cross Sectional Study ◽  
Adenomatous Polyps ◽  
Sectional Study ◽  
Cross Sectional ◽  
Korean Adults

scholarly journals Methylation Degrees of NIS and TIMP-3 Promoters in Well-Differentiated Thyroid Tumors

2020 ◽  
Author(s):  
Noman Arab ◽  
Maryam Zarkesh ◽  
Zahra Nozhat ◽  
Sara Sheikholeslami ◽  
Marziyeh Salehi ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Thyroid Cancer ◽  
Cpg Islands ◽  
Cross Sectional Study ◽  
Methylation Pattern ◽  
Mrna Levels ◽  
Cross Sectional ◽  
Normal Tissues ◽  
Well Differentiated ◽  
Promoter Dna

Abstract Background The aim of this study was to investigate the DNA methylation pattern in CpG islands of NIS and TIMP-3 promoters in thyroid cancers and the matched non-tumoral tissues as well as multinodular goiter (MNG) tissues. This cross-sectional study was performed on total 64 patients including 28 papillary thyroid cancer (PTC), 9 follicular thyroid cancer (FTC) and 27 MNG cases. Method: The bisulfite sequencing PCR technique was used to evaluate the promoter methylation pattern of the NIS and TIMP-3 genes. Results NIS mRNA levels were decreased in both of PTC and FTC tumoral tissues compared to their adjacent normal tissues (P = 0.04 and P = 0.03, respectively). TIMP-3 expression was also reduced in both PTC and FTC tumoral tissues compared to the adjacent non-tumoral tissues (P = 0.02 and P = 0.03, respectively). Moreover, a significant reduction in TIMP-3 expression was observed in FTC tissues compared to the MNG samples (P = 0.05). The methylation on NIS promoter was not a common event in PTC samples, but it was frequent in FTC (P < 0.05). A significant hyper-methylation was shown in TIMP-3 promoter in both PTC and FTC tissues compared to the non-tumoral and MNG samples (P < 0.05). Conclusion Aberrant promoter DNA methylation suggests potential utility to differentiate benign and malignant PTC and FTC tissues for early diagnosis, and contribution for personalized clinical management and surveillance. According to the reversibility of DNA methylation, these events may be potential targets for demethylating treatments.

scholarly journals Clinical profile and colonoscopic findings in patients with lower gastrointestinal haemorrhage: a descriptive cross-sectional study

2020 ◽  
Vol 11 (4) ◽  
pp. 40-45
Author(s):  
Subash Bhattarai ◽  
Ramesh Raj Acharya ◽  
Bishnu Jwarchan ◽  
Dipesh Karki
Keyword(s):  
Invasive Procedure ◽  
Gastrointestinal Haemorrhage ◽  
Colorectal Polyp ◽  
Cross Sectional Study ◽  
Clinical Profile ◽  
Gi Tract ◽  
Hemodynamic Stability ◽  
Cross Sectional ◽  
Carcinoma Of Colon ◽  
The Common

Background: Most of the bleeding in the lower gastrointestinal tract are usually located in the rectum, colon and terminal ileum. Colonoscopy is an invasive procedure used for both diagnostic and therapeutic purposes for detection of lower gastrointestinal (GI) tract pathologies and haemorrhage. Aims and Objective: The purpose of the study was to understand the clinical profile and colonoscopic findings in patients with lower gastrointestinal haemorrhage. Materials and Methods: Seventy-two patients presenting with lower GI haemorrhage were included in the study. All patients underwent colonoscopy after achieving hemodynamic stability and bowel preparation. Clinical profile and colonoscopic findings were studied. Results: The common aetiologies of lower GI haemorrhage were haemorrhoids followed by nonspecific colitis, colorectal polyp and carcinoma of colon. Rectum followed by sigmoid harbored majority of pathologies that presented with lower GI haemorrhage. Conclusions: The diagnosis of the pathological lesion and management of underlying cause not only prevents another episode of lower GI haemorrhage but also help in reducing morbidity and mortality. Colonoscopy or at least sigmoidoscopy is strongly recommended for evaluation, diagnosis and management of lower GI haemorrhage.

scholarly journals Tuberculosis in captive Asian elephants (Elephas maximus) in Peninsular Malaysia

2013 ◽  
Vol 141 (7) ◽  
pp. 1481-1487 ◽  
Author(s):  
B. L. ONG ◽  
Y. F. NGEOW ◽  
M. F. A. ABDUL RAZAK ◽  
Y. YAKUBU ◽  
Z. ZAKARIA ◽  
...  
Keyword(s):  
Close Contact ◽  
Cross Sectional Study ◽  
Peninsular Malaysia ◽  
High Seroprevalence ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Asian Elephants ◽  
Specific Pcr ◽  
Latent Tb ◽  
Polymerase Chain

SUMMARYA cross-sectional study was conducted from 10 January to 9 April 2012, to determine the seroprevalence of tuberculosis (TB) of all captive Asian elephants and their handlers in six locations in Peninsular Malaysia. In addition, trunk-wash samples were examined for tubercle bacillus by culture and polymerase chain reaction (PCR). For 63 elephants and 149 elephant handlers, TB seroprevalence was estimated at 20·4% and 24·8%, respectively. From 151 trunk-wash samples, 24 acid-fast isolates were obtained, 23 of which were identified byhsp65-based sequencing as non-tuberculous mycobacteria. TheMycobacterium tuberculosis-specific PCR was positive in the trunk-wash samples from three elephants which were also seropositive. Conversely, the trunk wash from seven seropositive elephants were PCR negative. Hence, there was evidence of active and latent TB in the elephants and the high seroprevalence in the elephants and their handlers suggests frequent, close contact, two-way transmission between animals and humans within confined workplaces.

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