scholarly journals Ameliorative Effect and In-Vivo Antioxidant Properties of Methanol Extract of Burkea africana Stem Bark On Acetaminophen-Induced Hepatotoxicity in Rats

2020 ◽  
Vol 4 (2) ◽  
pp. 36-42
Author(s):  
Terhemen Swem ◽  
◽  
Patrick Aba ◽  
Samuel Udem ◽  
Keyword(s):  
Methanol Extract ◽  
Antioxidant Properties ◽  
Stem Bark ◽  
Ameliorative Effect

scholarly journals Evaluation of Hepatoprotective and in vivo Antioxidant Potentials of Methanol Extract of Tephrosia pumila against Thioacetamide Induced Liver Toxicity in Wistar Rats

2021 ◽  
Vol 70 (2) ◽  
Author(s):  
Ramesh C ◽  
Pinkey Rawal ◽  
Soma Pramanik ◽  
Shabana S
Keyword(s):  
Biochemical Parameters ◽  
Methanol Extract ◽  
Liver Toxicity ◽  
Antioxidant Properties ◽  
Total Duration ◽  
Hepatoprotective Activity ◽  
Oral Toxicity ◽  
Standard Drug ◽  
Protein Ions

The objective of the current investigation was performed to assess the hepatoprotective potentials and in vivo antioxidant properties of methanol extract of Tephrosia pumila against thioacetamide induced liver damage in rats. The acute oral toxicity study of methanol extract was determined as per OECD guidelines and the extract was proved to be safe up to the dose of 2000mg/kg. The total duration of the study was 21 days and animals were divided into six groups. Hepatotoxicity was induced in the animals of all groups except normal control by single dose administration of Thioacetamide(100mg/kg) at first day of the study followed by animals were treated daily with standard drug sylimarin and methanol extract of Tephrosia pumila (100mg/kg, 200mg/kg and 400mg/kg) to respective groups for 21 days. Variations in biochemical parameters like alanine transferase (ALT), aspartate transferase (AST), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, albumin, total protein, ions and others parameters like clotting time and weight of the liver were considered to determine beneficial effect of the extract. At the end of the study liver samples were collected and subjected to histopathological evaluation. There were significant variations in the above mentioned biochemical parameters in toxic control animals treated with Thioacetamide alone while in the animals treated with methanol extract and standard drug silymarin, all the parameters were normal possibly due to their beneficial property in protecting the liver against thioacetamide induced hepatotoxicity. The results obtained in the above study suggesting that, the methanol extract of Tephrosia pumila possess significant hepatoprotective activity.

Anti-Snake Venom, Anti-Arthritic and Cytotoxic Activities of Tectona grandis L. f. Stem Bark (Lamiaceae)

2020 ◽  
Vol 35 (2) ◽  
pp. 87-94
Author(s):  
Adebayo Gbolade ◽  
Oluwasegun Adedokun ◽  
Ogochukwu Ume ◽  
Johnkennedy Onyechege ◽  
Cynthia Mkparu
Keyword(s):  
Snake Venom ◽  
Methanol Extract ◽  
Tectona Grandis ◽  
Stem Bark ◽  
Cytotoxic Activities ◽  
Standard Drug ◽  
Snake Bites ◽  
Vacuum Liquid Chromatography ◽  
Proteinase Inhibition

Tectona grandis L. f. (Lamiaceae) is famous for timber production and has been used in traditional medicine for treating bronchitis, liver-related  roubles, urinary discharge amongst other diseases. Methanol extract of  the stem bark was investigated for in vivo antiophidian assay against Bitis arietans and Naja nigricollis. Also, the extract and chromatographic fractions were subjected to cytotoxicity tests using tadpole model and  antiarthritic assay by proteinase inhibition. Chromatography of crude methanol extract afforded three highly polar vacuum liquid chromatography (VLC) fractions (BVLC-1, BVLC-2 and BVLC-3). BVLC-2 further gave four semi-pure uncharacterized isolates (I, II, III, IV). Only BVLC-2 and BVLC-3 were cytotoxic at 10 - 80 mg/ml, with BVLC-3 being most potent (100% mortality, LC50 40 mg/ml). Concentration-dependent proteinase enzyme inhibition (24 - 71%) at 200 - 1000 μg/ml of BVLC-2 was observed, and this was less (IC50 659.24 μg/ml) than the activity of the standard drug, acetyl salicylic acid. However, neutralization of B. arietans and N. nigricollis snake venoms using methanol extract was not dose-dependent, but the extract atthe least dose, 50 mg/kg offered better protection (75%) on Naja nigricollis envenomed-mice in 48 h. Its activity was comparable to that of the positive antivenin tested at 0.2 mg/kg. These findings justify the folkloric use of T. grandis in the treatment of snake bites, arthritic conditions, and oxidative stress-induced diseases. Keywords: Tectona grandis, methanol extract, anti-snake venom activity, tadpole cytotoxicity, anti-arthritic activity

In vitro antioxidant and in vivo antidiabetic potential of the methanol extract of Ficus glumosa Del (Moraceae) stem bark in alloxan-induced diabetic mice

2010 ◽  
Vol 21 (4) ◽  
pp. 389-394 ◽  
Author(s):  
Ifeanyi Innocent Madubunyi ◽  
Samuel Okwudili Onoja ◽  
Isaac Uzoma Asuzu
Keyword(s):  
Methanol Extract ◽  
Stem Bark ◽  
Diabetic Mice

scholarly journals Antimalarial Activity ofCroton macrostachyusStem Bark Extracts againstPlasmodium berghei In Vivo

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Jackie K. Obey ◽  
Moses M. Ngeiywa ◽  
Paul Kiprono ◽  
Sabah Omar ◽  
Atte von Wright ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Antimalarial Activity ◽  
Stem Bark ◽  
Significant Suppression ◽  
Innovative Drug ◽  
Croton Macrostachyus ◽  
Different Doses

There is an increasing need for innovative drug and prophylaxis discovery against malaria. The aim of the present study was to testin vivoantiplasmodial activity ofCroton macrostachyusH. (Euphorbiaceae) stem bark extracts from Kenyan folkloric medicine. Inbred Balb/c mice were inoculated with erythrocytes parasitized withPlasmodium berghei(ANKA). Different doses (500, 250, and 100 mg/kg) ofC. macrostachyusethyl acetate, methanol, aqueous, and isobutanol extracts were administrated either after inoculation (Peters’ 4-day suppressive test) or before inoculation (chemoprotective test) of the parasitized erythrocytes. All the extracts showed significant suppression of parasitemia compared to control (p<0.001): for the ethyl acetate extract in the range of 58–82%, for the methanol extract in the range of 27–68%, for the aqueous extract in the range of 24–72%, and for the isobutanol extract in the range of 61–80%. Chemoprotective effect was significant (p<0.001) and the suppression caused by the ethyl acetate extract was between 74 and 100%, by the methanol extract between 57 and 83%, and by the isobutanol extract between 86–92%. The study showed that it is possible to inhibit the growth of the parasites by various stem bark extracts ofC. macrostachyusin Balb/c mice supporting the folkloric use of the plant against malaria.

scholarly journals IN VITRO AND IN VIVO ANTI-TRYPANOSOMAL ACTIVITIES OF METHANOL EXTRACT OF AZADIRACHTA INDICA STEM-BARK

2017 ◽  
Vol 14 (6) ◽  
pp. 72-77
Author(s):  
Everlyne N Wanzala ◽  
Nicholas K Gikonyo ◽  
Grace Murilla ◽  
Mercy Githua ◽  
Ahmed Hassanali
Keyword(s):  
Azadirachta Indica ◽  
Methanol Extract ◽  
Stem Bark

In vivo Antioxidant and Hepato-Protective Properties of Stem Bark Methanol Extract of Vitex doniana

2020 ◽  
Vol 4 (1) ◽  
pp. 36-40
Author(s):  
Simon C. Mailafiya ◽  
Sherifat O. Kolawole ◽  
Abdulazeez K. Adeniyi ◽  
Bala A. Muhammed ◽  
Abdulfatai Ismail ◽  
...  
Keyword(s):  
Liver Damage ◽  
Methanol Extract ◽  
Stem Bark ◽  
Negative Control ◽  
Albino Rats ◽  
Albumin Concentration ◽  
Adult Rats ◽  
Control Drug ◽  
Vitex Doniana

Abstract The harmful effects that accompany the use of orthodox antioxidant medicine have necessitated the hunt for inherent antioxidants from plants extracts. In the present study, the in vivo antioxidant and hepato-protective activities of Vitex doniana against carbon tetrachloride (CCl4) induced liver damage in albino rats were investigated. The hepato-protective activities of the methanol extract of Vitex doniana stem bark were compared with Silymarin, a known hepatoprotective drug. Twenty-five (25) male albino adult rats were grouped into five (5) each. Group 1 and 2 was used as the normal and negative control respectively. Group 3-5 were treated with 200 mg/kg, 400 mg/kg methanol extract of Vitex doniana stem bark and 100 mg/kg Silymarin respectively. Results indicated that elevated levels of serum ALT, AST and ALB, and reduced serum SOD, GST and CAT in CCl4-hepatotoxic rats was an evidence of impairment in liver function. Administration of methanol extract of Vitex doniana stem bark (200 and 400 mg/kg body weight) and standard control drug Silymarin (100 mg/kg) have no significant (P>0.05) effect on CCl4- induced elevations of the ALT and AST levels while the reduction in albumin concentration, total proteins, SOD, GST and CAT due to CCl4 was reversed. In conclusion, Vitex doniana exhibited significant antioxidant and hepatoprotective properties in CCL4 induced liver damage in rat, and thus could be used and incorporated in the development of new and effective antioxidant drugs.

scholarly journals In vivo antiplasmodial and in vitro antioxidant properties of stem bark extracts of Haematostaphis barteri

2015 ◽  
Vol 5 (6) ◽  
pp. 446-450 ◽  
Author(s):  
Johnson Nyarko Boampong ◽  
Akua Afryie Karikari ◽  
Elvis Ofori Ameyaw
Keyword(s):  
Antioxidant Properties ◽  
Stem Bark

scholarly journals Biopotential of Bersama abyssinica Fresen Stem Bark Extracts: UHPLC Profiles, Antioxidant, Enzyme Inhibitory, and Antiproliferative Propensities

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 163 ◽  
Author(s):  
Kouadio Ibrahime Sinan ◽  
Annalisa Chiavaroli ◽  
Giustino Orlando ◽  
Kouadio Bene ◽  
Gokhan Zengin ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Hct116 Cell ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Stem Bark ◽  
Scientific Data ◽  
Water Extracts ◽  
Bersama Abyssinica

In this study, ethyl acetate, methanol, and water extracts of Bersama abyssinica (Melianthaceae) stem bark were screened for enzyme inhibitory and antioxidant properties. The water extract possessed the highest concentration of phenols (230.83 mg gallic acid equivalent/g extract), while the methanol extract was rich in flavonoids (75.82 mg rutin equivalent/g extract), and the ethyl acetate extract possessed the highest amount of saponins (97.37 mg quillaja equivalent/g). The aim of this study was to investigate the antiproliferative effects against the human colon cancer HCT116 cell line challenged with serotonin (5-HT) as a stimulating-proliferation factor. The level of HCT116 cell-deriving pool of kynurenic acid (KA) was also assessed. The UHPLC results confirmed the presence of 58, 68, and 63 compounds in the ethyl acetate, methanol, and water extracts, respectively. Mangiferin, vitexin and its isomer isovitexin were tentatively identified in all extracts and KA (m/z 190.05042 [M−H]+) was also tentatively identified in the methanol and water extracts. The methanol extract (1464.08 mg Trolox equivalent [TE]/g extract) showed the highest activity in the CUPRAC assay, whereas the water extract (1063.70 mg TE/g extract) showed the highest activity with the FRAP technique. The ethyl acetate extract was the most active acetylcholinesterase (4.43 mg galantamine equivalent/g extract) and α-glucosidase (mmol acarbose equivalent /g extract) inhibitor. The water extract was able to inhibit 5-HT-stimulated viability of HCT116 cells, and blunt 5-HT-induced reduction of cell-deriving KA. The scientific data generated in this study provide baseline data regarding the biological properties of B. abyssinica stem bark, highlighting its potential use for the development of new pharmaceutic and cosmetic agents.

scholarly journals Evaluation of Pharmacological and Phytochemical Profiles of Piptadeniastrum africanum (Hook.f.) Brenan Stem Bark Extracts

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 516 ◽  
Author(s):  
Kouadio Ibrahime Sinan ◽  
Annalisa Chiavaroli ◽  
Giustino Orlando ◽  
Kouadio Bene ◽  
Gokhan Zengin ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Ethyl Acetate ◽  
Type Ii Diabetes ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Stem Bark ◽  
Type Ii ◽  
Water Extracts ◽  
Skin Hyperpigmentation ◽  
Phytochemical Profiles

The stem bark (SB) of Piptadeniastrum africanum (PA) has been extensively used in African traditional medicinal systems. However, there is a dearth of scientific information regarding its possible activity in the management of type II diabetes, Alzheimer’s disease, and skin hyperpigmentation disorders. This study therefore attempted to elucidate the in vitro inhibitory action of ethyl acetate, methanol, and water extracts of P. africanum stem bark (PA-SB) on α-amylase, α-glucosidase, acetylcholinesterase, butyrylcholinesterase, and tyrosinase. Cell viability, catecholamine, and 3-hydroxykynurenine levels of hypothalamic HypoE22 cells exposed to PA-SB extracts were also investigated. The phytochemical profiles of the extracts were determined by high performance liquid chromatography (HPLC) and antioxidant properties were investigated. Saponin (867.42 mg quillaja equivalent/g) and tannin (33.81 mg catechin equivalent/g) contents were higher in the methanol extract. Multiple dihydroxy-trimethoxy(iso)flavone isomers, loliolide, eriodictyol, naringenin, luteolin, chrysoeriol, apigenin, and liquiritigenin, were characterized from PA-SB extracts using HPLC. The methanol extract of PA-SB showed highest inhibitory activity against acetylcholinesterase (4.88 mg galantamine equivalent (GALAE)/g extract), butyrylcholinesterase (5.37 mg GALAE/g extract), and tyrosinase (154.86 mg kojic acid equivalent/g extract) while α-glucosidase was effectively inhibited by the ethyl acetate extract (15.22 mmol acarbose equivalent/g extract). The methanol extract of PA-SB also showed potent antioxidant properties (493.87, 818.12, 953.07, and 732.19 mg Trolox equivalent/g extract, for 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP) assays, respectively). PA-SB extracts exhibited antioxidant activity and promising inhibition against key enzymes related to type II diabetes, Alzheimer’s disease, and skin hyperpigmentation disorders. Additionally, all extracts were able to contrast hydrogen peroxide-induced oxidative stress, in HypoE22 cells, thus restoring basal catecholamine and 3-hydroxykinurenine levels, whereas only methanol and water extracts stimulated basal dopamine release. Overall, data from the present study contribute to the biological assessment of P. africanum that appears to be a promising source of natural compounds with protective and neuromodulatory effects.

scholarly journals Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of Entada abyssinica Stend ex A. Satabie

2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Gerald N Teke ◽  
Paul K Lunga ◽  
Hippolyte K Wabo ◽  
Jules-Roger Kuiate ◽  
Gerard Vilarem ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Antioxidant Properties ◽  
Stem Bark
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