scholarly journals In Vitro Antiamoebic Activity of Cnestis ferruginea and Dianthus basuticus on Entamoeba histolytica Trophozoites

2021 ◽  
Vol 5 (3) ◽  
pp. 555-558
Keyword(s):  
Entamoeba Histolytica ◽  
Antiamoebic Activity

scholarly journals Antiprotozoal Activity Against Entamoeba histolytica of Flavonoids Isolated from Lippia graveolens Kunth

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2464
Author(s):  
Ramiro Quintanilla-Licea ◽  
Javier Vargas-Villarreal ◽  
María Julia Verde-Star ◽  
Verónica Mayela Rivas-Galindo ◽  
Ángel David Torres-Hernández
Keyword(s):  
Entamoeba Histolytica ◽  
Inhibitory Concentration ◽  
Public Health Problem ◽  
In Vitro Tests ◽  
Antiprotozoal Activity ◽  
Subsequent Work ◽  
Antiamoebic Activity ◽  
Work Up ◽  
First Time

Amebiasis caused by Entamoeba histolytica is nowadays a serious public health problem worldwide, especially in developing countries. Annually, up to 100,000 deaths occur across the world. Due to the resistance that pathogenic protozoa exhibit against commercial antiprotozoal drugs, a growing emphasis has been placed on plants used in traditional medicine to discover new antiparasitics. Previously, we reported the in vitro antiamoebic activity of a methanolic extract of Lippia graveolens Kunth (Mexican oregano). In this study, we outline the isolation and structure elucidation of antiamoebic compounds occurring in this plant. The subsequent work-up of this methanol extract by bioguided isolation using several chromatographic techniques yielded the flavonoids pinocembrin (1), sakuranetin (2), cirsimaritin (3), and naringenin (4). Structural elucidation of the isolated compounds was achieved by spectroscopic/spectrometric analyses and comparing literature data. These compounds revealed significant antiprotozoal activity against E. histolytica trophozoites using in vitro tests, showing a 50% inhibitory concentration (IC50) ranging from 28 to 154 µg/mL. Amebicide activity of sakuranetin and cirsimaritin is reported for the first time in this study. These research data may help to corroborate the use of this plant in traditional Mexican medicine for the treatment of dyspepsia.

scholarly journals In Vitro Study of Antiamoebic Activity of Methanol Extracts ofArgemone mexicanaon Trophozoites ofEntamoeba histolyticaHM1-IMSS

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Joel H. Elizondo-Luévano ◽  
Rocío Castro-Ríos ◽  
Eduardo Sánchez-García ◽  
Magda E. Hernández-García ◽  
Javier Vargas-Villarreal ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Methanolic Extract ◽  
Parasitic Infection ◽  
In Vitro Study ◽  
Argemone Mexicana ◽  
Traditional Use ◽  
Antiparasitic Activity ◽  
Antiamoebic Activity ◽  
Amoebicidal Activity

Infections caused by parasites in humans represent one of the main public health concerns. Amoebiasis, a parasitic infection caused byEntamoeba histolytica(E. histolytica), is considered endemic in Mexico, whereArgemone mexicana(A. mexicana) has been used in traditional medicine to treat intestinal parasitic diseases. The objective of this work was to evaluate the potential biological activity ofA. mexicanaonE. histolytica. For this purpose, a methanolic extract was prepared fromA. mexicanaleaves, and a differential fractionation was carried out with solvents of different polarities. The inhibitory capacities of the extract and its fractions were evaluated in vitro using HM1-IMSS, a strain ofEntamoeba histolytica.A. mexicanaextract was found to have a growth-inhibiting activity forE. histolytica, showing IC50 = 78.39 μg/mL. The extract was characterized phytochemically, and the methanolic extract fractions were analyzed by liquid chromatography (HPLC) and mass spectrometry (MS). Berberine and jatrorrhizine were present in the active fractions, and these compounds may be responsible for the antiparasitic activity. The identification of amoebicidal activity ofA. mexicanaonE. histolyticagives support to the traditional use. Further studies with berberine and jatrorrhizine will be carried out to understand the mechanism involved.

scholarly journals Activity of Extracts and Procesterol from Calotropis gigantea against Entamoeba histolytica

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Shailendra Singh ◽  
Neelam Bharti ◽  
Manoj Chugh ◽  
Fehmida Naqvi ◽  
Amir Azam
Keyword(s):  
Entamoeba Histolytica ◽  
Chromatographic Separation ◽  
Chloroform Extract ◽  
Root Bark ◽  
Inhibitory Effects ◽  
Calotropis Gigantea ◽  
Growth Inhibitory ◽  
Antiamoebic Activity

Extracts from the root bark of Calotropis gigantea were subjected to bioactivity-guided fractionation using growth inhibitory effects against Entamoeba histolytica. The n-hexane soluble portion of the chloroform extract showed in vitro antiamoebic activity against the HK-9 strain of Entamoeba histolytica. Chromatographic separation of the chloroform extract afforded the known compound, procesterol, which showed activity against E. histolytica.

scholarly journals In vitro and in vivo Antiamoebic Potential of Actinopyga lecanora (Jaeger)

2015 ◽  
Vol 18 (2) ◽  
pp. 118-120 ◽  
Author(s):  
Vijai Lakshmi ◽  
Sheela Ghosal
Keyword(s):  
Entamoeba Histolytica ◽  
Symptomatic Relief ◽  
Pharmaceutical Journal ◽  
Climatic Conditions ◽  
Isolation And Characterization ◽  
Histolytica Infection ◽  
Antiamoebic Activity

Human amoebiasis, due to Entamoeba histolytica infection, is mainly associated with morbidity thus affecting the quality of life and pace of development in the countries with warm climatic conditions. So far, the available drugs provide only symptomatic relief and they are not devoid of side effects. This leads to obtain novel molecules from natural sources having antiamoebic activity. The methanol extract of Actinopyga lecanora (Jaeger) displayed antiamoebic activity. It showed MIC 125 ?g/ml in our in-vitro studies, but when it was tested in rats, it revealed 88% inhibition of trophozoites at the dose of 900 mg/kg body weight against Entamoeba histolytica. Further work is in progress for the isolation and characterization of active molecules.Bangladesh Pharmaceutical Journal 18(2): 118-120, 2015

scholarly journals In vitro study of antiamoebic activity of methanol extract of fruit of Pimpinella anisum on trophozoites of Entamoeba histolytica HM1-IMSS

2013 ◽  
Vol 12 (16) ◽  
pp. 2065-2068
Author(s):  
Y Quintilde ones Gutieacute rrez ◽  
M J Verde Star ◽  
C Rivas Morales ◽  
Or ◽  
A ay Caacute rdenas ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Methanol Extract ◽  
In Vitro Study ◽  
Vitro Study ◽  
Pimpinella Anisum ◽  
Antiamoebic Activity

Bioactive chemical compounds identified in extracts of Reseda sphenocleoides leaves and evaluation of antiamoebic activity in vitro

2021 ◽  
Vol 19 ◽  
Author(s):  
Mansour Abdulnabi H. Mehdi ◽  
Mohammad Mohsin ◽  
Fadel Y. S. Alarabi ◽  
Gozif Mohammed N. Omar ◽  
Mazahar Farooqui ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Aqueous Extract ◽  
Ethanolic Extract ◽  
Chemical Compounds ◽  
Aqueous Extracts ◽  
Histolytica Infection ◽  
Phytochemical Compounds ◽  
Ft Ir ◽  
Antiamoebic Activity

Background: Entamoeba histolytica is a causative agent of amoebiasis, estimated to cause more than 100,000 deaths per year. Metronidazole is used to treatment of E. histolytica infection. However, this drug has several untoward side effects, the current study was designed to analysis of the bioactive chemical products in extracts of Reseda sphenocleoides leaves and evaluation of anti-amoebic activity in vitro. Methods: Bioactive chemical compounds were tests by GC-MS and FT-IR. Entamoeba histolytica was cultured under xenic conditions in Locke's egg (LE) medium and different concentrations of extracts of R. sphenocleoides were added to cultivated parasites. Results: 11 and 18 bioactive phytochemical compounds were showed in the ethanolic and aqueous extracts of R. sphenocleoides leaves respectively by GC-MS analysis. FT-IR analysis of extracts of R. sphenocleoides leaves proved the presence many functional groups for various phytocompounds. The ethanolic extract of R. sphenocleoides leaves was showed inhibits the growth of E. histolytica in vitro at concentrations 5 and 10 mg/ml after 96 hrs of incubation. While the highest concentration 20 mg/ml used in this study inhibited the E. histolytica in all times of incubation. The aqueous extract showed there was no growth of E. histolytica at the concentrations of 5 and 10 mg/ml after 72 and 48 hrs respectively. However, the higher concentfrations (15 and 20 mg/ml) of the aqueous extract were on the growth of the E. histolytica parasite during the period of incubation. It was found that the minimum inhibitory concentration (MIC) for ethanolic and aqueous extracts of R. sphenocleoides leaves was <15 mg/ml, <10 mg/ml respectively. Conclusion: The results specify that the extracts of R. sphenocleoides has a higher capacity in reducing of E. histolytica number in vitro. Moreover, these results showed that the extracts of R. sphenocleoides contains various bioactive compounds and therefore has various medicinal properties that can be used for the treatment of various diseases.

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