scholarly journals Assessment of bacteriological quality and Escherichia coli O157: H7 in ready-to-eat street foods

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 98-105
Author(s):  
S.R. Latchumaya ◽  
M.N.S.S. Hameed ◽  
P. Arkappan ◽  
R.J. Sreedharam ◽  
P. Sandrasaigaran
Keyword(s):  
Microbiological Quality ◽  
Total Coliform ◽  
Colony Count ◽  
Laboratory Service ◽  
The Public ◽  
E Coli ◽  
Bacteriological Quality ◽  
Legal Enforcement ◽  
Night Markets

Ready-to-eat (RTE) street foods are usually prepared well in advance (4-8 hrs) before the sale. Owning to minimal cooking procedures, cross-contaminations, and abused holding temperature and time, there is a high probability for deteriorating microbiological quality in these foods. Thus, this study was aimed to assess the bacteriological quality of RTE foods sampled from night markets and street stalls based on the guidelines provided by the Public Health Laboratory Service. A total of fifty samples (category 2, 3 and 4) were evaluated for aerobic colony count, total coliform, and E. coli. The pathogenic strain E. coli O157: H7 were examined using the multiplex PCR technique. It was noticed that category 3 and 4 RTE street foods were largely unsatisfactory for the coliform (>104 CFU/ mL) and E. coli count (>100 CFU/mL). In opposite, category 2 RTE street foods were unsatisfactory for the aerobic colony count (>105 CFU/mL) and E. coli (>100 CFU/ mL). However, there was no E. coli O157: H7 or Shiga-toxin producing bacteria reported in this study. The statistical analysis showed that in overall, category 3 and 4 RTE street foods were significantly at worsening bacteriological quality compared to category 2. Strict legal enforcement and amending the existing rules are needed to improve the quality of RTE street foods sold in the night markets and street stalls.

Microbiological Quality of Non-Dairy Creamers, Fillings and Toppings

1986 ◽  
Vol 49 (8) ◽  
pp. 621-622 ◽  
Author(s):  
DONALD W. WARBURTON ◽  
KARL F. WEISS
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Colony Count ◽  
Bacteriological Quality

A study conducted in 1984–1985, in the province of Ontario, Canada, assessed the bacteriological quality of three types of non-dairy substitutes including creamers, fillings and toppings. All sample units tested contained acceptable levels of aerobic colony count (ACC), yeast/mold and aerobic sporeformers. Escherichia coli, Staphylococcus aureus and Salmonella were not detected in any of the 79 lots tested, indicating that good hygienic practices were used during the manufacture of these products.

scholarly journals Bacterial Contamination of Drinking Water in Guadalajara, Mexico

2018 ◽  
Author(s):  
Francesca Rubino ◽  
Yahaira Corona ◽  
José Guadalupe Jiménez Pérez ◽  
Charlotte D. Smith
Keyword(s):  
Drinking Water ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
E Coli ◽  
Chlorine Residual ◽  
Piped Water ◽  
Regulatory Limits ◽  
Access To Water

In many regions where drinking water supply is intermittent and unreliable, households adapt by storing water in cisterns or rooftop tanks. Both intermittent supply and stored water can be vulnerable to contamination by microorganisms with deleterious health effects. The Guadalajara Metropolitan Area is a rapidly growing urban center with over five million residents where household storage is nearly ubiquitous. This pilot study was conducted in July 2018 to examine the microbiological quality of drinking water in Guadalajara. Samples were tested for free available chlorine residual, total coliform bacteria, and E. coli. A survey on access to water and public perspectives was also conducted. Water exiting rooftop tanks exceeded regulatory limits for total coliform levels in half of the homes studied. Piped water arriving at two homes had total coliform levels that far exceeded regulatory limits. No E. coli were detected in any of the samples. Only 35% of homes had a chlorine residual between the recommended 0.2 and 1.5 mg/L. Many homes reported unpleasant odors and colors. Only 7% of residents drank the piped water. Future studies are needed, especially during April and May when many homes reported a higher disruption to water service.

scholarly journals Evaluation of Physical Chemical and Biological Characteristics of Underground Wells in Badra City, Iraq

2019 ◽  
Vol 16 (3) ◽  
pp. 0560
Author(s):  
Kadhim Hashim Yaseen Al-Araji
Keyword(s):  
Total Coliform ◽  
Plate Count ◽  
The Public ◽  
E Coli ◽  
Quality Of Water ◽  
Advance Method ◽  
Pollution Detection ◽  
Private Wells ◽  
Positive Results

The quality of groundwater should be improved by keeping safe water sources from contaminants in protective way by doing regular measuring and checkup before it supplied for usage. Private Wells do not receive the same services that wells supplying the public do. Well owners are responsible for protecting their drinking water. This work was carried out in Badra city, Iraq from December 2017 to May 2018, six wells water were investigated to determine the general characteristics of wells as well as studying the effect of environmental factors on the quality of water. The average of six wells were eleven parameters that is out of permissible limits were EC, Sal., Alk., TH, TDS, Na, Ca, Cl, SO4, Fe, Zn (4402-5183 /cm, 2.76-3.9 ppt, 302-366mg/L, 3164-4248 mg/L, 604-675 mg/L, 375-524 mg/L, 635-871 mg/L, 631-1107 mg/L, 2430-4570 g/L, 114-392 g/L). Respectively, microbiological investigations involved the total coliform, total plate count, as well as the detection for the presence of E. coli, Salmonella and Cholera.  Results shows that there is a significant relation between the increasing of the TDS and Turbidity, TDS gives an indication for the significant increasing of chemical ions. Wells number 3, 4 and 5 showed gave positive results for E.coli growth which as a source of microbial pollution. Detection for the presence of chemical and microbial contaminate is an important alarm since this water has a direct effect on the human and animal's health. Advance method of rapid detection for the well water quality is highly recommended to avoid any health issue and prevent the outbreak of health risk and ecological contaminants.

scholarly journals Bacterial Contamination of Drinking Water in Guadalajara, Mexico

2018 ◽  
Vol 16 (1) ◽  
pp. 67 ◽  
Author(s):  
Francesca Rubino ◽  
Yahaira Corona ◽  
José Guadalupe Jiménez Pérez ◽  
Charlotte Smith
Keyword(s):  
Drinking Water ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
E Coli ◽  
Chlorine Residual ◽  
Piped Water ◽  
Regulatory Limits ◽  
Access To Water

In many regions where drinking water supply is intermittent and unreliable, households adapt by storing water in cisterns or rooftop tanks. Both intermittent supply and stored water can be vulnerable to contamination by microorganisms with deleterious health effects. The Metropolitan Zone of Guadalajara is a rapidly growing urban center with over five million residents where household storage is nearly ubiquitous. This pilot study was conducted in July 2018 to examine the microbiological quality of drinking water in Guadalajara. Samples were tested for free available chlorine residual, total coliform bacteria, and Escherichia coli. A survey on access to water and public perspectives was also conducted. Water exiting rooftop tanks exceeded regulatory limits for total coliform levels in half of the homes studied. Piped water arriving at two homes had total coliform levels that far exceeded regulatory limits. No E. coli were detected in any of the samples. Only 35% of homes had a chlorine residual between the recommended 0.2 and 1.5 mg/L. Many homes reported unpleasant odors and colors. Only 7% of residents drank the piped water. Future studies are needed, especially during April and May when many homes reported a higher disruption to water service.

scholarly journals The effect of container-biofilm on the microbiological quality of water used from plastic household containers

2003 ◽  
Vol 1 (3) ◽  
pp. 101-108 ◽  
Author(s):  
P. Jagals ◽  
C. Jagals ◽  
T. C. Bokako
Keyword(s):  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Human Consumption ◽  
Microbiological Contamination ◽  
Supply Source ◽  
E Coli ◽  
Quality Of Water ◽  
Storage Containers

Studies in Southern Africa have shown that even when microbiologically safe water is supplied to developing communities at communal standpipes, contamination by high numbers of pathogenic microorganisms may occur during the processes of fetching water from the supply source and storage during use at home, rendering such waters unsafe for human consumption. This study investigated the occurrence of biofilm in PVC storage containers as one possible reason for this deterioration, using heterotrophic bacteria and total coliform counts as well as turbidity as indicators. A second objective was to determine whether biofilm in water-storage containers could contribute to hazardous microbiological contamination indicated by Escherichia coli and Clostridium perfringens. Results indicated that increased microbiological contamination is associated with biofilm. The biofilm harbours heterotrophic bacteria, total coliforms and C. perfringens. E. coli could not be associated directly with the levels of biofilm in containers but rather appears to be introduced intermittently from the ambient domestic environment. When dislodged with the biofilm, these bacteria contributed substantially to the deterioration of the microbiological quality of supplied water stored in plastic containers.

Microbiological Quality of Dry Dessert Mixes Sold in Canada

1987 ◽  
Vol 50 (2) ◽  
pp. 136-140 ◽  
Author(s):  
D.W. WARBLTRTON ◽  
P.I. PETERKIN ◽  
G.A. JARVIS ◽  
K.F. WEISS ◽  
G. RIEDEL
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Cereus ◽  
Microbiological Quality ◽  
Colony Count ◽  
Practical Consideration ◽  
Bacteriological Quality

A study done in 1977–1978, assessed the bacteriological quality of five types of dry desserts including starch-, gelatin- and rennet- based products. One hundred and ninety-seven lots were randomly selected across Canada and analyzed for aerobic colony count, aerobic sporeformers, Bacillus cereus, coliforms, Escherichia coli, Staphylococcus aureus and Salmonella. Micro-biological and practical consideration do not warrant the establishment of standards or guidelines for such products at this time.

scholarly journals Sources of microbiological contamination in sachet water from Ghana

2020 ◽  
Vol 10 (2) ◽  
pp. 202-208
Author(s):  
Asli Aslan ◽  
Haresh Rochani ◽  
Oghenekpaobor Oyibo ◽  
J. Edward Dotherow ◽  
Kendall W. Anderson ◽  
...  
Keyword(s):  
Drinking Water ◽  
Quantitative Polymerase Chain Reaction ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Primary Sources ◽  
Source Tracking ◽  
E Coli ◽  
Sewage Contamination ◽  
Sachet Water

Abstract Sachet water is one of the primary sources of drinking water in rapidly growing countries. A study to assess the microbiological quality of sachet water in 21 different brands was conducted in Ghana. Culturable total coliform was positive in 87% of the samples collected, where Escherichia coli colonies were absent. The analysis of quantitative polymerase chain reaction results indicated the presence of E. coli genes in 44.6% of the samples, with the highest concentration up to 3,166 CCE/100 ml. Microbial source tracking analyses showed that the source of E. coli genes did not originate from sewage contamination because the human-associated HF183 marker was not detected. Of the 175 samples tested, 71% did not mention any water treatment before filling the packages. These results suggest non-human sources of contamination, such as biofilm formation in the pipelines used to fill these packages due to poor disinfection. Our study shows an urgent need for increased regulation and standardized manufacturing of sachet water to ensure safe drinking water.

scholarly journals A survey of the microbiological quality of private water supplies in England

2000 ◽  
Vol 124 (3) ◽  
pp. 417-425 ◽  
Author(s):  
M. RUTTER ◽  
G. L. NICHOLS ◽  
A. SWAN ◽  
J. DE LOUVOIS
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Water Quality ◽  
Uv Light ◽  
Microbiological Quality ◽  
Light Treatment ◽  
Water Supplies ◽  
The Public ◽  
E Coli

Results from statutory testing of private water supplies in nine Public Health Laboratories in England were compiled, and the effects of supply class, source, treatment and location on water quality were examined. A total of 6551 samples from 2911 supplies was examined, over a 2-year period, of which 1342 (21%) samples, and 949 (33%) supplies on at least one occasion, failed current regulations for Escherichia coli. Total coliforms, including E. coli, were detected in 1751 (27%) samples from 1215 (42%) supplies. The percentage of samples positive for E. coli was highest in summer and autumn, and lowest in winter. Samples taken from larger supplies and from boreholes were less frequently contaminated than those from other sources. Chlorination, filtration or UV light treatment improved the bacteriological quality of supplies, but still resulted in a low level of compliance with the regulations. The public health implications of the study are discussed.

scholarly journals Bacterial Contamination of Drinking Water in Guadalajara, Mexico

2018 ◽  
Author(s):  
Francesca Rubino ◽  
Yahaira Corona ◽  
José Guadalupe Jiménez Pérez ◽  
Charlotte Smith
Keyword(s):  
Drinking Water ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
E Coli ◽  
Chlorine Residual ◽  
Piped Water ◽  
Regulatory Limits ◽  
Access To Water

In many regions where drinking water supply is intermittent and unreliable, households adapt by storing water in cisterns or rooftop tanks. Both intermittent supply and stored water can be vulnerable to contamination by microorganisms with deleterious health effects. The Guadalajara Metropolitan Area is a rapidly growing urban center with over five million residents where household storage is nearly ubiquitous. This pilot study was conducted in July 2018 to examine the microbiological quality of drinking water in Guadalajara. Samples were tested for free available chlorine residual, total coliform bacteria, and E. coli. A survey on access to water and public perspectives was also conducted. Water exiting rooftop tanks exceeded regulatory limits for total coliform levels in half of the homes studied. Piped water arriving at two homes had total coliform levels that far exceeded regulatory limits. No E. coli were detected in any of the samples. Only 35% of homes had a chlorine residual between the recommended 0.2 and 1.5 mg/L. Many homes reported unpleasant odors and colors. Only 7% of residents drank the piped water. Future studies are needed, especially during April and May when many homes reported a higher disruption to water service.

scholarly journals Physicochemical and Bacteriological Quality of Public Swimming Pools in the Tamale Metropolis, Ghana

J ◽  
2020 ◽  
Vol 3 (2) ◽  
pp. 236-249
Author(s):  
Umar Farouk Mustapha ◽  
Seth Mensah Abobi ◽  
Gerard Quarcoo
Keyword(s):  
Staphylococcus Aureus ◽  
Heterotrophic Bacteria ◽  
Total Coliform ◽  
Faecal Coliform ◽  
Health Concern ◽  
Swimming Pools ◽  
E Coli ◽  
Bacteriological Quality ◽  
Tamale Metropolis

The study focused on the physicochemical and bacteriological quality of public swimming pools in the Tamale Metropolis. Physicochemical properties such as pH, temperature, and conductivity, and bacteria counts—including total coliform, faecal coliform, Escherichia coli (E. coli), Staphylococcus aureus, and total heterotrophic bacteria—were analyzed for their conformity with required health standards. The results obtained were analyzed using Student t test and compared with World Health Organization (WHO) and Environmental Protection Agency (EPA) standards for safe recreational and drinking water. The highest and the lowest temperatures were recorded in April (32.53 °C) and February (28.16 °C), respectively. The lowest and the highest mean pH values were 4.04 and 6.13, which were below acceptable standards. The conductivity level varied from 469.1563 µS cm−1 to 928.1563 µS cm−1. While the pH did not conform to acceptable standards, temperature and conductivity were within the EPA and/or the WHO acceptable limits. The total coliform (TC) expressed in colony-forming units per 100 mL ranged from 0 to 397 (cfu/100 mL), faecal coliform (FC) 0 to196 cfu/100 mL, E. coli 0 to 52 cfu/100 mL, Staphylococcus aureus (S. aureus) 8 to 27 cfu/100 mL, and Total Heterotrophic Bacteria (THB) 44 to 197 (cfu/mL). TC, FC, E. coli, S. aureus, and THB counts in most of the samples complied with the bacteriological standards. However, the bacterial loads increased and exceeded the WHO and/or EPA standards as the number of bathers increased. Besides, there were positive correlations between physicochemical parameters such as temperature, pH, and bacterial loads. Most parameters studied met the acceptable standards of recreational water stipulated by the WHO and EPA. However, the presence of pathogenic organisms in the recreational waters at any point in time should be treated as a public health concern, and hence a call for routine monitoring and inspection of public swimming pools in the Tamale Metropolis.

Sign in / Sign up

Export Citation Format

Share Document